FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22263096
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAT4153261.865535682907714No Hit
GGTACC3344901.5024415292464264No Hit
AACATA3324381.4932244823451328No Hit
TCATGT3244181.4572007415320851No Hit
CCACTT3207231.4406037686761983No Hit
CTGAGG3138881.4099027376965No Hit
GGCTAC2929911.3160388833610563No Hit
CATGAG2860891.2850369059182065No Hit
GAGCAC2830891.2715616911502337No Hit
TTCTAC2826461.2695718511028296No Hit
ACTAGC2799311.257376781737814No Hit
GCACAT2771791.2450155180573268No Hit
GCTGTC2769101.2438072404664653No Hit
GTGGTG2663221.1962487158120327No Hit
GACACG2555241.1477469261238418No Hit
TTGAAT2541891.1417504555520939No Hit
ACCAGG2511901.1282797325223768No Hit
GTTGGC2343521.0526478437680005No Hit
GAAGAT2255681.0131924149273757No Hit
GATCAG2255051.0129094354172483No Hit
GGACTA2248051.0097652186380546No Hit
ACTTGA2232031.0025694539519572No Hit
CCATGA2194550.9857344189685029No Hit
TCAATG2134840.9589142498419807No Hit
CGTGAA2029620.9116521799124434No Hit
GCTTCT2020720.9076545328646114No Hit
TTAGTA2019710.9072008673007564No Hit
AAGGCG1972040.8857887510344473No Hit
CCTGCT1947920.874954678360997No Hit
CGTTGC1946390.8742674424078304No Hit
TCGTGG1921860.8632492084658845No Hit
AATTGC1905210.8557704642696595No Hit
AGGACT1895130.8512427921076207No Hit
TCCTCC1854620.8330467604326011No Hit
CACATT1853070.8323505410029224No Hit
GACTAA1777870.7985726693178702No Hit
CAGGAC1766720.7935643811624402No Hit
GCCAAT1741050.7820340890593114No Hit
ACACGA1739830.7814860969920805No Hit
TAGCTT1722010.7734818194199046No Hit
ATTGCT1719500.7723543931176509No Hit
GATTCA1709120.7676919688079322No Hit
GAGAAG1682590.7557753872147881No Hit
GAGATA1681910.7554699490133807No Hit
CAGCCT1669160.7497429827369921No Hit
ACCTGC1647400.7399689602919558No Hit
GTAAGA1622600.7288294494170982No Hit
TGACCA1618470.7269743615173738No Hit
TTACGT1611910.7240277812214437No Hit
CGAGCC1585260.7120572987692279No Hit
ATTCGA1545680.6942789987520155No Hit
CCTTAT1545140.6940364448861919No Hit
TCCACG1533720.6889068797978501No Hit
CTATTA1492290.6702976082032795No Hit
CGCTTG1491600.6699876782636162No Hit
GTCTCA1450810.6516658779174289No Hit
GCGTAT1449550.651099918897174No Hit
ACAGTG1426400.6407015448345549No Hit
CATTAA1422810.6390890108006541No Hit
TGCGGA1413890.6350823802763101No Hit
CCGCTC1403270.6303121542484478No Hit
CGATGT1396800.6274059995968216No Hit
CATAAC1390730.624679514475435No Hit
CAAGCT1386120.6226088231394232No Hit
AAGAGG1381970.6207447517631869No Hit
GACCGA1374090.6172052620174661No Hit
AGGCTT1367910.6144293677752636No Hit
AATGTA1352990.607727694297325No Hit
CACCTA1351700.6071482600623023No Hit
AACTAG1327510.5962827452210601No Hit
AAGTTG1326930.5960222244022125No Hit
ATGCCG1313660.5900616877365125No Hit
TTAGGC1312880.5897113321525452No Hit
TGTGTG1301910.584783895285723No Hit
AGATGC1294430.5814240750702417No Hit
CTGGAG1281780.5757420261764132No Hit
GAGCCA1279950.5749200380755669No Hit
TAGAGC1270450.5706528867323755No Hit
GAATAC1239810.556890200716019No Hit
TACGGC1226090.5507275358287994No Hit
ATGGCC1211350.5441067136394686No Hit
CAAGGA1202070.539938380537909No Hit
GGCCAG1194370.536479742080796No Hit
ATCCAC1175420.527967898085693No Hit
CAGTAG1167090.5242262801184525No Hit
CAGATC1143670.5137066291229216No Hit
GCCTGG1136860.5106477553705918No Hit
AACCTT1098920.4936061004273619No Hit
GCGGCT1078970.48464508260665995No Hit
ACAGCC1077550.4840072557743092No Hit
AGTCAA1071030.4810786424314031No Hit
TATTCT1068850.48009944349159706No Hit
TACAGT1043240.46859610181800415No Hit
GATCCT1031170.463174573743023No Hit
CGAATT1013830.4553858996071346No Hit
TATTGG993950.4464563239542245No Hit
TTCGTT979020.43975015873803No Hit
AACTCT964830.43337638215277874No Hit
CTTAGC951200.427254142909863No Hit
CCTCGG949630.42654894000367244No Hit
CGAGAG936150.42049407683459655No Hit
GAGTCG928440.4170309466392275No Hit
CTGTTC925050.4155082473704466No Hit
GCCATA912270.40976780587929007No Hit
TGCATA902680.4054602288917948No Hit
AGTTAG900360.4044181456164048No Hit
GGATCG897200.4029987563275117No Hit
CAGTCC887010.3984216750446568No Hit
TCCAGA868350.39004009145897767No Hit
ATCCGG867210.3895280332977947No Hit
GTACGC860590.38655450257232865No Hit
CAACGG852340.38284881851113606No Hit
GGATTC828430.37210907234106166No Hit
CATACG811050.3643024312521493No Hit
CTCGTC789990.3548428304850323No Hit
CCGTCG784250.3522645727260934No Hit
ACGAAC781550.3510518033969759No Hit
GTACTT768740.3452978866910514No Hit
AGACGG738880.3318855562586623No Hit
TATGGT725380.3258217096130745No Hit
GGCCGT696960.31305618948954805No Hit
GTTACA686780.3084835999449492No Hit
TCGGAC682370.3065027433740572No Hit
CTTCAC670950.3013731782857155No Hit
GGCGTT660630.2967377044055328No Hit
CGTCAG654200.2938495167069306No Hit
ACGTAA652390.29303651208259623No Hit
AGCCTA648740.29139702761915953No Hit
ACCGCT630450.283181638348952No Hit
CGAAGC629870.2829211175301045No Hit
TAAGTC627140.281694872986219No Hit
AGTGCA619300.2781733501935221No Hit
ACGCTA610280.2741218022866182No Hit
TACGAA589930.2649811149356765No Hit
GGTAAT587790.26401988294889445No Hit
AAGCGC587320.2638087712508629No Hit
AGTTCC585680.26307212617688036No Hit
TCAAGC585470.26297779967350454No Hit
AATCGG585360.26292839055268863No Hit
TTAGCG585260.2628834731701287No Hit
GAGTGA580320.2606645544716692No Hit
GACTTG574100.2578706932764428No Hit
CCGACT555710.24961038662367532No Hit
TTCCAT552710.24826286514687806No Hit
AAGCAT537290.24133660475613994No Hit
GGCAGG534250.2399711163263187No Hit
CCTGAC531830.23888411566836887No Hit
TAATGA524180.23544793590253577No Hit
GCATAA522970.23490443557356083No Hit
GGTGGT520480.23378599274781908No Hit
AACAAT513080.23046210643838574No Hit
AGTAGG492310.22113276608069246No Hit
GGAGGC488370.219363021207832No Hit
GAACCG470390.21128687582356023No Hit
TGACAC467300.20989892870245896No Hit
TGGCAT453550.2037227886004714No Hit
GTCGAG449660.2019755024188909No Hit
TACTCG410130.1842196610929585No Hit
TCTCTG381310.17127447143919247No Hit
CTATGC352220.15820800485251468No Hit
CTCCGC347240.15597111920103116No Hit
CGACAA340110.1527685098245096No Hit
TCTCGC322840.14501127785641316No Hit
AGGTGT310780.139594241519688No Hit
CGTCTC257540.11568022704479197No Hit
AGAGGT241280.10837666064055063No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)