FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19245107
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGC3841841.9962684541062827No Hit
ACTAAT3543321.8411537020812614No Hit
GAGCAC3517801.8278931886427028No Hit
CTGTAT3391991.7625207279959525No Hit
AGGACT3347111.7392005147074527No Hit
TGATAA3236861.6819132260475351No Hit
GCTTCT3139001.6310639374465414No Hit
ATTGAA2992111.554738043285496No Hit
TCAATG2817321.4639149577084711No Hit
TGTGTG2765221.4368431414800655No Hit
GACCGA2726001.416463935482406No Hit
GTCTGC2705091.405598836109355No Hit
ATTCAT2661221.3828034315423656No Hit
CGTTGC2610211.3562979930431147No Hit
TCTTCA2254011.1712119865064923No Hit
AACATA2171471.1283231628693986No Hit
TTCGTT2024471.0519401113228417No Hit
CGTACT1982751.0302618738362952No Hit
ACCAGG1954571.0156191908935606No Hit
AAGTTG1928351.0019949486381134No Hit
AATGTA1919070.9971729437513649No Hit
CATTAA1913290.9941695829490582No Hit
TTCTGT1839150.9556455051146248No Hit
GCGCAG1804690.9377396550718061No Hit
AAGCGC1781460.9256690544770678No Hit
TATAAG1767870.9186075193034781No Hit
GCTGTC1751660.9101845991295345No Hit
GTAAGA1736080.9020890348907906No Hit
GACACG1735680.9018811898525688No Hit
GACTAA1730670.8992779307488392No Hit
GAGATA1728760.8982854706913296No Hit
CTAGTT1704580.8857212381308143No Hit
GCTCAA1675540.8706316883559027No Hit
CTTAGC1633530.8488027632166452No Hit
ATACCT1594850.8287041480205851No Hit
GACCAT1594040.8282832618181859No Hit
GGACTA1587270.8247654845462797No Hit
TCCAGA1579290.8206189760337523No Hit
ATCAGC1538820.7995902542916492No Hit
GGCCGT1517320.7884185834872209No Hit
GGTAAT1478060.7680185929857392No Hit
CATGAG1448480.752648452409228No Hit
GGTACC1422250.7390190140278254No Hit
CCAGCG1408210.7317236531862359No Hit
ATGTGA1404900.7300037354949495No Hit
CGAATT1371630.7127162244408409No Hit
TGCGAC1362730.7080916723404032No Hit
TTCACA1351000.7019966165945454No Hit
CACATT1347870.7003702291704588No Hit
GAAGAT1326850.6894479724118967No Hit
TTGAAT1280570.6654003014896201No Hit
TTACGT1280140.6651768680735316No Hit
CCGCTC1271250.6605575120990493No Hit
GCGTAT1248750.6488662286990662No Hit
CTACCG1245280.647063172992491No Hit
CAGGAC1234900.6416695942506321No Hit
TACTCG1234760.6415968484872544No Hit
TGCGGA1206800.627068480315542No Hit
TCCTCC1194310.6205785189970624No Hit
GTTGGC1175020.6105551920288103No Hit
TGCATA1156780.6010774582858905No Hit
TATTCT1154460.5998719570642034No Hit
GTCGAG1152620.5989158698883825No Hit
AGAACG1120690.5823246397123175No Hit
CGTCTC1087990.5653333078376753No Hit
GGCTAC1047840.5444708621261497No Hit
ATCCGG1035070.5378354092809149No Hit
CTGTTC1023740.5319481985732789No Hit
AACCTT1018140.529038368038172No Hit
CCGGAA1001370.5203244648107178No Hit
GCGGCT993780.5163806052104569No Hit
CTCCGC946830.4919847938491586No Hit
AGGTCA941630.48928280835227367No Hit
GAGAAG927530.4819562707549509No Hit
AATCGG925420.48085988817833025No Hit
ACGAAC912550.47417247407353985No Hit
ATCCAC898160.4666952488235061No Hit
ACACGA890430.4626786434598675No Hit
ACCGCT877940.4561886821413879No Hit
GATCAG874670.45448954895392374No Hit
CGAGCC850770.4420708079201638No Hit
TAGCTT844160.43863616866354654No Hit
TAAGTC840290.43662526791874945No Hit
ACTTGA819830.42599399421369805No Hit
CTATTA818110.4251002605493438No Hit
TGACCA808460.42008599900223986No Hit
AGGTGT804570.41806470600553164No Hit
TCTCGC800330.41586154860037927No Hit
GTCTCA800060.41572125319957953No Hit
CATACG790810.4109148366906975No Hit
AAGCAT789750.41036404733940945No Hit
AGAGGT765050.3975296162292057No Hit
AAGGCG763720.39683853147711784No Hit
TGGCAT744800.38700746116922086No Hit
TCAAGC734060.3814268218929622No Hit
GCCAAT719780.3740067540284395No Hit
CAGCCT708060.3679168944085372No Hit
GGATTC702670.36511618251849676No Hit
CAAGGA694940.36109957715485813No Hit
AGTCAA686730.35683355774535314No Hit
TAGAGC678450.35253116545415936No Hit
TCCACG656820.34129194501230886No Hit
CGATGT642490.33384589651800844No Hit
TAATGA631280.32802103932183907No Hit
ACAGTG621670.32302756227855733No Hit
CTATGC608010.3159296542232787No Hit
GAATAC607370.3155971021621236No Hit
AACTAG602840.3132432571042603No Hit
TACAGT598380.31092578492808587No Hit
CCTTAT596120.309751460462132No Hit
CGTGAA596100.3097410682102209No Hit
TTAGGC574300.2984135136271261No Hit
CAGATC562390.2922249276140683No Hit
GCACAT559210.29057255956020406No Hit
CGAGAG551600.286618307708032No Hit
AACTCT542740.28201454011141636No Hit
CAGTAG538270.27969187180928634No Hit
TTAGTA534540.2777537168278669No Hit
GTACTT531910.2763871357015578No Hit
CGTCAG518690.2695178571883233No Hit
GAGCCA508320.26412947457241986No Hit
ACAGCC507990.2639580024158868No Hit
GTACGC499210.2593958038269156No Hit
TGTGAT496810.25814873359758406No Hit
CCTGCT494590.25699519363545237No Hit
TATTGG485490.25226671901590364No Hit
TCTCTG465270.2417601523337854No Hit
AGTTCC458390.23818521767636833No Hit
GGATCG457840.2378994307488132No Hit
GATCCT450320.233991944030241No Hit
CCATGA437810.2274915904598504No Hit
AGACGG436470.22679530958180696No Hit
CCTCGG435360.22621853960074112No Hit
GAGTCG429900.2233814548290119No Hit
CATAAC424070.2203521133969273No Hit
TTAGCG421950.21925053469435113No Hit
CTTCAC411860.2140076436052031No Hit
ATGCCG410520.2133113627271597No Hit
TACGGC410210.2131502828225377No Hit
ACGCTA408060.21203311574209488No Hit
ATGGCC403510.20966887843232046No Hit
CCACTT402470.20912848133294348No Hit
TCGTGG397570.20658237961472492No Hit
AGCCTA387830.2015213529340211No Hit
CAACGG381650.1983101470934924No Hit
AGTAGG370270.19239695575607868No Hit
GCATAA369760.19213195333234573No Hit
GTGGTG369090.191783812893324No Hit
GGCGTT368590.1915240065955466No Hit
CCTGAC361170.18766848113652992No Hit
GGAGGC359480.1867903358500423No Hit
CTCGTC358440.18624993875066528No Hit
GTTACA348640.18115773531422819No Hit
TATGGT346430.18000939147805206No Hit
AAGAGG343000.17822712027529905No Hit
GGTGGT332490.17276599189601805No Hit
GGCCAG328280.1705784228687323No Hit
ACGTAA328240.1705576383649101No Hit
TTCTAC327210.17002243739148865No Hit
AACAAT327050.16993929937619986No Hit
TGACAC322240.16743996279158124No Hit
CACCTA321760.16719054874571496No Hit
AGGCTT317370.16490944945122935No Hit
CCGACT317140.16478993855425172No Hit
CCGTCG317010.1647223889168296No Hit
AGTTAG310410.16129294578616787No Hit
ACCTGC307690.15987959952625883No Hit
AGATGC306670.1593495946787929No Hit
CAAGCT303750.15783232589977286No Hit
GAGTGA300920.15636182225435274No Hit
CTGAGG293260.15238158977240293No Hit
GATTCA284880.14802723622165365No Hit
GGCAGG273640.1421867906476176No Hit
TCATGT265870.13814940078015675No Hit
AATTGC245630.12763244184612743No Hit
GCCATA245340.12748175419341654No Hit
CAGTCC241090.1252734006623086No Hit
ATTGCT240510.12497202535688681No Hit
CGCTTG229280.11913677590880632No Hit
ATTCGA220670.11466291146107943No Hit
TCGGAC218240.11340025285388124No Hit
GACTTG210920.10959668865442006No Hit
GAACCG205220.10663489685975765No Hit
TTCCAT203090.1055281220312259No Hit
CGAAGC201020.10445252395842747No Hit
AGTGCA195710.10169338107603144No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)