FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001836090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304453570
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGA35163381.154966913345769No Hit
AACATA31432291.0324165356313608No Hit
GGTACC29915540.9825977734470317No Hit
TGTGAT28801130.9459941625910316No Hit
CTTAGC27954640.9181905799298068No Hit
TTACGT27765920.9119919336140483No Hit
CATGAG26408550.8674081240039327No Hit
AGGACT26245520.862053284512315No Hit
CAGATC25876440.84993058219025No Hit
CCTGCT25534740.8387071959773703No Hit
TTCTAC25132830.8255061683132834No Hit
GTACTT24878890.8171653234350315No Hit
TCGTGG24821970.8152957444381421No Hit
ATGGCC24411670.8018191410926795No Hit
GCTCAA24207900.7951261665284464No Hit
CCGTCG24149210.7931984505880486No Hit
AAGCGC24136660.7927862366665629No Hit
GCTGTC24119250.7922143924934104No Hit
GAGTCG24034900.7894438551007958No Hit
TAGAGC23941610.786379676874868No Hit
GGTAAT23462720.7706501848541306No Hit
AACTAG23435260.7697482410864815No Hit
CACATT23204750.7621769716807723No Hit
CTGTAT23018970.756074891813553No Hit
GAAGAT22478600.7383260442634981No Hit
ACTAAT22350310.7341122654597217No Hit
CAAGGA22181580.7285702053025688No Hit
CGAATT22092940.7256587597248408No Hit
ACCAGG21959160.7212646578589964No Hit
ACTAGC21814280.71650596838132No Hit
AACCTT21609650.7097847464886026No Hit
TAGCTT21571730.7085392363768308No Hit
TACGGC21424190.703693177255238No Hit
CGTACT21139660.6943475814719465No Hit
GGCCGT21112900.6934686297158545No Hit
CCTTAT20934370.687604681396904No Hit
CGTTGC20530120.6743267947227553No Hit
CAGGAC20210970.6638440797393179No Hit
ATTGAA20166510.6623837585481425No Hit
GAATAC19959740.6555922467915223No Hit
GAGATA19842520.6517420702276541No Hit
TGGCAT19797080.6502495602202989No Hit
GCACAT19726660.6479365638576681No Hit
GATCAG19460770.6392032124964079No Hit
CCTCGG19363820.636018818895768No Hit
CTGAGG19362830.6359863016222802No Hit
CCGGAA19332410.634987134491476No Hit
TTGAAT19075290.6265418401892939No Hit
TACAGT19045880.6255758472465933No Hit
GTCGAG18770140.6165189654369959No Hit
TGATAA18700900.6142447270367039No Hit
ATGCCG18443300.6057836667837397No Hit
CGAAGC18063330.5933032744533099No Hit
CAGCCT18042510.5926194263381441No Hit
GTAAGA17993480.5910090001572326No Hit
GGACTA17992170.5909659722498902No Hit
GTCTCA17912510.5883494813347073No Hit
CATACG17785040.5841626360301835No Hit
CTGGAG17720090.5820293058150049No Hit
GAGAAG17669820.5803781509279067No Hit
CAGTCC17341640.5695988389953844No Hit
ACACGA17296830.5681270217984306No Hit
CTGTTC17281970.5676389342388068No Hit
CGTGAA17102130.5617319580125141No Hit
CTATTA17053660.560139925440848No Hit
GCGCAG16980330.5577313480016017No Hit
GTGGTG16979300.5576975168988821No Hit
TTAGTA16863800.5539038349919825No Hit
GAGCAC16861630.553832559756156No Hit
CCAGCG16831260.5528350349118915No Hit
TGCATA16314130.5358495221455278No Hit
CAACGG16105910.5290103840792539No Hit
CCACTT15915670.522761812252686No Hit
ATTCAT15830410.5199613852450473No Hit
CAGTAG15675020.5148574871367085No Hit
GGCTAC15591590.512117167816426No Hit
TCTTCA15150720.49763647048053994No Hit
ACGAAC15140040.49728567807564217No Hit
ACAGCC14946590.4909316714532203No Hit
AGTTAG14871960.4884803945639396No Hit
GACTTG14856520.4879732564804545No Hit
TCATGT14749050.48444332579184407No Hit
ATCCGG14612440.47995627050784784No Hit
AAGAGG14589390.47919917641300774No Hit
GCCATA14518840.47688191010537334No Hit
GGCCAG14432480.4740453527938595No Hit
CGAGCC14370450.4720079321126043No Hit
GCTTCT14350420.471350032124767No Hit
TCAATG14172050.46549133912274376No Hit
AAGGCG14153820.46489256145033875No Hit
AATCGG14119720.46377252203020647No Hit
CCATGA14070190.46214567298389697No Hit
TCCAGA13833030.45435597946839645No Hit
GACACG13722030.4507101033500773No Hit
GTTGGC13330210.4378404891097188No Hit
TCCACG12595930.41372252590107583No Hit
GTCTGC12366010.40617063547653587No Hit
AACTCT12138850.39870939926899196No Hit
GAACCG11956710.39272687786186905No Hit
TAATGA11210540.3682183789140656No Hit
CTATGC10878740.35732016543606304No Hit
TCTCTG10656990.3500366246321237No Hit
CTCCGC10276970.33755459001515403No Hit
TAAGTC10123970.3325291932034169No Hit
GACTAA9797290.3217991498670881No Hit
TGTGTG9597450.31523525902488186No Hit
AATTGC9285410.30498607718740167No Hit
AGTGCA9173180.30129980082020386No Hit
TGACCA8971990.2946915682414235No Hit
CATAAC8913600.2927737060202644No Hit
TTCCAT8844270.2904965115042008No Hit
AAGTTG8774300.28819829572042793No Hit
TATAAG8716620.2863037539681338No Hit
ACCGCT8701510.2858074549758113No Hit
AAGCAT8671120.2848092732169309No Hit
GTACGC8664340.2845865791621363No Hit
GACCGA8619680.28311968882480176No Hit
TGCGAC8597840.2824023380642244No Hit
GCCAAT8525370.2800220079534623No Hit
CGCTTG8484550.2786812452223832No Hit
TTAGGC8253310.271085998433193No Hit
TCGGAC8222300.2700674523212193No Hit
ATTGCT8157070.26792492530141787No Hit
TTCGTT8153980.2678234319932593No Hit
TACGAA8081120.2654302920474869No Hit
ACAGTG7974980.2619440461808347No Hit
TATTCT7917570.2600583727758554No Hit
CGTCTC7831280.25722411466549727No Hit
GGTGGT7731420.2539441399882419No Hit
CGATGT7672750.25201708096246006No Hit
CTTCAC7651290.25131221157958505No Hit
CAAGCT7591110.24933555550030176No Hit
AGTCAA7532750.2474186786510666No Hit
CCGCTC7508110.2466093598442613No Hit
TTAGCG7492810.2461068201630876No Hit
TCAAGC7457430.24494473820753687No Hit
AATGTA7445520.24455354555376047No Hit
CTACCG7379230.24237620205931565No Hit
TTCTGT7368350.24201884050825878No Hit
GATCCT7328570.24071223733720715No Hit
CGAGAG7112140.2336034358211007No Hit
GACCAT7105890.23339815000362782No Hit
GCCTGG7017690.23050115654744993No Hit
TACTCG6880670.2260006345138275No Hit
CTAGTT6798490.22330137235703953No Hit
GCATAA6772050.2224329312348021No Hit
TCTCGC6607920.21704196143930912No Hit
CACCTA6598430.21673025545405822No Hit
TATTGG6564010.21559970540007134No Hit
GGATCG6560220.21547522008035577No Hit
ATTCGA6555990.21533628263909008No Hit
TATGGT6459650.21217192493423548No Hit
CATTAA6427160.2111047671406842No Hit
GCGTAT6354600.20872148091415055No Hit
GGAGGC6271620.20599594217272604No Hit
ACGCTA6189420.20329602310132214No Hit
AGAGGT6156370.20221047169852532No Hit
AGGCTT6099490.20034220653086773No Hit
GAGCCA6023740.19785414242309593No Hit
AGGTGT6009420.19738379155810193No Hit
ACCTGC6009380.19738247772887013No Hit
ATCAGC5949220.19540647856420273No Hit
GGCGTT5876000.19300151415534395No Hit
CGTCAG5855390.19232456364364525No Hit
CCTGAC5843830.1919448669956473No Hit
CTCGTC5798820.19046648065253433No Hit
AGATGC5793880.19030422274240372No Hit
GATTCA5777530.18976719504389455No Hit
AGAACG5749960.188861638245858No Hit
ATCCAC5700690.18724332908955543No Hit
AGACGG5616820.1844885576477228No Hit
CGACAA5612900.18435980238300376No Hit
AGCCTA5530460.1816520003362089No Hit
GGCAGG5510430.18099410034837168No Hit
TTCACA5376960.1766101806590739No Hit
AACAAT5327550.17498727310045994No Hit
AGTTCC5319200.17471301124831612No Hit
GAGTGA5309210.17438488239766742No Hit
ATGTGA5207280.171036917057665No Hit
GGATTC5183770.17026471392665884No Hit
ATACCT5057670.16612286727332512No Hit
ACGTAA4804530.15780829897970977No Hit
AGTAGG4786640.15722068885577528No Hit
TGACAC4476800.1470437676260456No Hit
GTTACA4417760.14510455567986935No Hit
TCCTCC4359870.14320311632410812No Hit
TGCGGA4226650.1388274080675093No Hit
CCGACT4124910.13548568341635805No Hit
AGGTCA4068330.13362727196793914No Hit
GCGGCT3856130.12665740789309846No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)