FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26983618
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGC6519362.4160436899158593No Hit
TATTCT5934862.1994307805572997No Hit
AATCGG5848112.1672816447371885No Hit
ACCGCT5184621.9213954185091118No Hit
AACATA5164271.9138538056683134No Hit
GACTAA4997711.8521274648936996No Hit
CGTCTC4950601.8346687238160575No Hit
TACTCG4888381.8116102888797194No Hit
AGGTGT4583981.6988011022094962No Hit
GTAAGA4163341.5429139265164515No Hit
GGATTC4062971.5057172837237764No Hit
GTCGAG4047111.4998396434458863No Hit
AGAGGT3952761.4648739839112754No Hit
TCCAGA3859101.4301640350823228No Hit
TCTCGC3683981.365265399176641No Hit
ACCAGG3682091.3645649742002723No Hit
AGTCAA3659271.3561079911522613No Hit
GGTAAT3653701.3540437757457136No Hit
CCGCTC3440661.275092168885581No Hit
ATCCAC3314201.2282266966572088No Hit
GACACG3264651.2098637032291222No Hit
ATGTGA3260891.208470265180896No Hit
CGAATT3186071.180742330402098No Hit
GGCCGT3112981.1536555253635743No Hit
TCCTCC3095141.147044106539012No Hit
GCGTAT2922831.0831868432172438No Hit
CATGAG2863061.0610363665836064No Hit
AATTGC2852651.057178470285193No Hit
GAGATA2801451.0382039947348796No Hit
GCTGTC2733671.0130850503442497No Hit
AAGCGC2663640.9871322666960376No Hit
GGACTA2554840.9468115061516214No Hit
AACTCT2551200.9454625395304662No Hit
TGCGAC2492940.9238716616874728No Hit
TAATGA2474540.9170527095365788No Hit
TGCGGA2457700.9108118859376084No Hit
TTCTAC2372910.8793891167596577No Hit
GTACGC2356340.8732483538715972No Hit
GTTGGC2298540.8518279498323761No Hit
CTGGAG2282090.8457316583713866No Hit
TGGCAT2234000.8279097339726645No Hit
ACGCTA2220940.8230697603264321No Hit
CTTAGC2173500.8054887228243447No Hit
CACATT2162230.8013121146319222No Hit
GCCTGG2074060.7686367335914702No Hit
TAAGTC2071300.7676138907688361No Hit
CTTCAC2044390.7576411732481537No Hit
CTGAGG2019120.7482762318974424No Hit
TTGAAT1988330.7368656049014628No Hit
AACCTT1976790.7325889359981304No Hit
CCTGAC1920040.7115576569457809No Hit
AGTTCC1919320.7112908283833547No Hit
TTAGCG1901240.7045904667046502No Hit
TTCACA1895380.7024187786826808No Hit
CCAGCG1804430.6687131429150828No Hit
AAGCAT1796060.6656112608768772No Hit
TACAGT1768270.6553124195576738No Hit
TCTCTG1744270.6464181341434644No Hit
GGCAGG1735500.643168014015022No Hit
GTACTT1712870.6347814440598737No Hit
TTACGT1699880.6299674120794327No Hit
TTCTGT1664480.6168483410934739No Hit
TAGAGC1632430.6049707641132482No Hit
ATTGCT1621320.6008534511569205No Hit
ACGAAC1599320.5927003561938952No Hit
AGAACG1592950.5903396646068737No Hit
TCAAGC1574270.583416945792814No Hit
CATACG1565530.5801779435211394No Hit
CTGTTC1563620.5794701066402586No Hit
CTCGTC1527730.5661694439937595No Hit
AAGGCG1504150.5574308085742986No Hit
GCGGCT1502130.5566822062186028No Hit
CAGCCT1444510.5353285093199881No Hit
TCTTCA1430620.5301809416365144No Hit
GAGAAG1393560.5164466825760726No Hit
CGTGAA1384400.5130520303096494No Hit
ATCCGG1377850.5106246315820214No Hit
CCACTT1368500.5071595662227356No Hit
TGTGAT1349980.5002961426447706No Hit
CGCTTG1349860.5002516712176995No Hit
CTATGC1349390.5000774914616712No Hit
CCGGAA1339060.4962492427813053No Hit
GCGCAG1335300.49485580473307916No Hit
CAAGGA1324600.49089043581924413No Hit
CGTACT1316810.48800349901188195No Hit
GATTCA1313880.4869176550008972No Hit
CTACCG1275750.4727868590490719No Hit
AGGTCA1257080.4658678461872681No Hit
CGTCAG1255830.46540460215527807No Hit
TGCATA1248380.4626436677246172No Hit
TGTGTG1246320.46188024155989754No Hit
ATTCGA1230640.456069308422614No Hit
GGAGGC1176700.43607940195417827No Hit
GTGGTG1110020.4113681123116997No Hit
GTCTCA1021350.3785074336584516No Hit
GAGCAC992020.3676378756918364No Hit
TCATGT986200.3654810114788906No Hit
GACCGA982240.3640134543855461No Hit
CTAGTT979060.3628349615681633No Hit
GCTCAA921000.34131820277028824No Hit
ATTCAT854170.31655132384397083No Hit
TATAAG838020.31056621095065906No Hit
TGACAC809860.30013024939798655No Hit
CATTAA779510.28888268430126757No Hit
AGGACT769510.28517673204534694No Hit
GTCTGC753990.2794250941441581No Hit
AATGTA731250.2709977587141947No Hit
AAGTTG726680.2693041385332389No Hit
TGATAA690380.25585153184424714No Hit
ATCAGC673460.24958106062722948No Hit
ACTAAT649440.24067936330850812No Hit
ATACCT648850.24046071212540884No Hit
GACCAT640920.23752189198646378No Hit
TTCGTT620220.22985057081670812No Hit
TCAATG587200.21761351646765828No Hit
CGTTGC458600.1699549704565192No Hit
GCTTCT452650.16774992886424644No Hit
ACTAGC450000.16676785151642748No Hit
CTATTA434850.16115333384870775No Hit
CTGTAT398260.14759325454429426No Hit
GATCAG362650.13439635856096094No Hit
GGTACC340490.12618396836184087No Hit
GAAGAT333850.1237232160639096No Hit
GATCCT323280.1198060245294015No Hit
ACACGA312890.11595554013549998No Hit
GCATAA306870.11372455687743577No Hit
CCTCGG301430.11170851885021497No Hit
TGACCA301290.11165663551863207No Hit
CGAGAG294400.1091032344143028No Hit
CAGATC290960.1078283868382661No Hit
CGATGT287380.1065016559306465No Hit
GCCAAT282340.10463385599366253No Hit
CAAGCT275630.10214716202993979No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)