FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001836102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408059200
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGTT34789190.8525525217909558No Hit
GTTGGTCG34013040.8335319973180362No Hit
GTATGCCT33969990.8324770033367707No Hit
TGCTGCCG32076640.7860780984719864No Hit
GGCGCCAT31788620.7790198088904747No Hit
GCCGTCCT31604010.7744957104263304No Hit
TGGCCTAT30375450.7443883142445997No Hit
CGTCCGAA30124300.7382335700310151No Hit
GCTCACGT29900580.7327510322031705No Hit
AGCTGTGG29610370.7256390739382913No Hit
AAGACACT28612730.7011906605708191No Hit
GAGTAATT28242930.6921282500186248No Hit
CAAGCACT26950150.6604470625830762No Hit
CCGGTCTT26677450.653764208722656No Hit
TAATGATT26092290.6394241325768417No Hit
TTGTACTC25906680.6348755278645843No Hit
CCAACAAT25737810.6307371577457388RNA PCR Primer, Index 36 (100% over 8bp)
CTCTATAG25519740.6253930802197328No Hit
GCCTGTGA25191270.6173435129020496No Hit
ATAACGCG25012900.6129723334261303No Hit
GTTCGGAC24777140.607194740371005No Hit
CCTTACTG24687630.6050011861024086No Hit
TCTCTATA24368140.5971716848927803No Hit
GGTCAACT24348840.596698714304199No Hit
CTGGCCTC24178640.5925277508753631No Hit
AGCCATTG24157810.5920172857271689No Hit
CTCTCTGC23971600.5874539772660438No Hit
ATCATGGA23577790.5778031716966557No Hit
GTCTGGCG23465400.5750489144712336No Hit
ATTATCGT23282660.5705706426910605No Hit
CGTTCGCG23037050.564551663092022No Hit
ACATTCCT23035090.5645036308457204TruSeq Adapter, Index 27 (100% over 8bp)
CAGCGTCT23028050.5643311068590048No Hit
GGAGAACC22615670.554225220262158No Hit
AACGAGAT22484240.551004364072664No Hit
CAGATTCA22458180.5503657312468386No Hit
TGGCTTCC22412640.5492497167077718No Hit
CCGTACAC22394790.5488122801789544No Hit
TCCTGGAA22368070.5481574732293746No Hit
GGCCTGCT22312680.5468000721463945No Hit
TCTGCGCA22305650.5466277932221599No Hit
CCAGCCAA22180490.5435605912083346No Hit
CGTGCCAC22173720.5433946839086093No Hit
AACTACCT21871650.5359920815411097No Hit
AGCATAGT21843970.5353137485933414No Hit
TCTACCGT21803720.5343273721067923No Hit
AGCACAAC21759830.5332517928771119No Hit
TGTACGCC21734950.5326420774240601No Hit
CGACAGTT21448130.5256131953402841No Hit
GTCCTCAA21408500.524642012727565No Hit
CTGTTCGC21192750.5193547896971812No Hit
CAATATGG20818130.5101742590290821No Hit
TACGTCAG20815180.5101019655971486No Hit
CCGTGGCG20678480.506751961480099No Hit
TAAGCAAC20556380.5037597485854994No Hit
CGCGCGCT20379620.49942802416904214No Hit
AGGATTGG20282850.4970565545391453No Hit
TGTAGACG20131920.4933578265114474No Hit
GTCATTAG20082080.4921364351054945No Hit
GAGACCAT20069860.4918369687535534No Hit
TTCACTTG20013440.49045432623501684No Hit
CCGACCGC19899320.4876576732003592No Hit
TAGCTGGT19663580.48188057027019604No Hit
ACGCGACC19637730.4812470837564745No Hit
CAACCGGT19588520.48004113128683285No Hit
ACGTGCGC19565810.4794845943921862No Hit
TCGTCTGG19529440.47859330214831575No Hit
CATTAAGT19469900.4771342001356666No Hit
GCCACGAC19435830.47629927226245605No Hit
AGGCGTGC19154480.4694044393558582No Hit
GCGCTGGC19114130.46841561224449785No Hit
AACGCTGG18947940.4643429188705952No Hit
CGCTGAAG18872710.4624993138250528No Hit
ACTAAGTG18692170.4580749557907284No Hit
AACTCTAC18628040.4565033700992405No Hit
CGAAGCAA18594660.4556853515372279No Hit
AACTAGTC18567810.45502735877539335No Hit
CACGGCAA18533270.45418091296556967No Hit
CTGATCCT18377980.45037533769609905No Hit
TCGAGCAA18129580.4442879856648251No Hit
GATATATG18043040.4421672149531245No Hit
CATCCTCA18014250.4414616800699506No Hit
ATCTGTCT18012410.4414165885734227No Hit
TCAGGTAC17984950.44074364700023916No Hit
ACTCGGAA17756380.4351422538690464No Hit
TTGAGAGT17729750.4344896524817968No Hit
GTCAACGT17599840.43130604578943443No Hit
GTCCAGAG17541570.42987806671188883No Hit
AAGTTACG17460690.4278960013645079No Hit
TATCTACT17448830.42760535726188753No Hit
CAATTAAG17310880.42422472033469655No Hit
GATGTCCA17227870.4221904566788348No Hit
CTGTAGCA17161600.4205664276163851No Hit
GAAGCGAT17128440.4197538004289574No Hit
CTGCTCAG17087700.418755415880833No Hit
ATGTTGTG17015920.4169963573912805No Hit
TAATTGAT16989250.4163427757541063No Hit
ACAGCACG16980510.4161285911455985No Hit
AGACAGAG16877030.4135926845908633No Hit
TATAACGG16852360.41298811544991515No Hit
CAGAGGAT16834170.4125423467967393No Hit
GCTCCAGA16776540.41113005171798606No Hit
CGGCCAAG16766260.4108781274873842No Hit
TGCCTGAC16695140.4091352431215863No Hit
CGCGTGAA16678880.4087367715272686No Hit
CTACGGCC16638650.40775088516568186No Hit
CTATGGAG16634160.4076408521116544No Hit
ACTTCTAT16450980.4031517975823116No Hit
GAGAGCGG16394830.4017757717507656No Hit
GGATACTA16336490.400346077235852No Hit
ACCAGCGG16308910.399670194912895No Hit
ATATTACA16263540.3985583464360073No Hit
CGCATATA16237820.39792804573454044No Hit
GTTGTGTG16196100.3969056450632653No Hit
GGTTGCAG16169310.3962491226763175No Hit
CTAACATA16125160.3951671718221278No Hit
GATTATCT16083790.39415334833769217No Hit
GGATCTCT16038590.39304566592298373No Hit
GGTGAGAT15992450.3919149476350491No Hit
CTTAGATT15932580.39044775856052255No Hit
TGCCAGGT15904360.3897561922387732No Hit
ATAGGAGG15900130.3896525308092551No Hit
CTTCCTGT15817360.38762414865294054No Hit
GGCACGTT15628610.3829985943216082No Hit
AATTGCTA15628480.38299540850935354No Hit
GTCAGATG15575440.381695597109439No Hit
CTCAGAAC15536010.3807293157463427No Hit
GCCTCATT15464880.3789861863180637No Hit
GACGCTCA15365440.37654928500570506No Hit
ATTGAGTA15263290.37404597176096016No Hit
ACCGTTCA15228540.37319437963903274No Hit
TGTACCTA15037770.36851932268651216No Hit
CATAATAA14462490.3544213682720546No Hit
CTCACGCC14416450.35329310060893127No Hit
CCAGATTC14344730.35153551249426557No Hit
AGTTCGTC14333710.35126545364006007No Hit
GGCCAATA14326080.3510784709669577No Hit
ACCTGAAC14310420.35069470312150786No Hit
GAGGTGCG14226670.34864230484204256No Hit
TTGGAACG14151450.3467989448589812No Hit
CGAACTGC14127280.3462066288420896No Hit
AGGAATAC14091720.3453351866591906No Hit
TAGAGTAG14054890.34443262154118814No Hit
TATAATCC14028400.3437834510286743No Hit
CCTTAGCC13987140.3427723232315311No Hit
TTGTCACA13965390.34223931233507293No Hit
GGATCAGA13901200.34066625626869823No Hit
AGATGCGA13895710.34053171696655776No Hit
GGTGCGGA13871050.33992739288809076No Hit
CAACGTAA13745080.3368403408132937No Hit
CGAACGAG13727100.33639971847222166No Hit
TTCGGAAG13605150.333411181514839No Hit
TCGCGGAT13560500.33231697753659273No Hit
CCAATCTA13534140.33167099283633356No Hit
AACCTTCT13355110.32728363923665976No Hit
TGAGGCGG13173300.3228281582672318No Hit
TGCATCAA13164540.32261348353376174No Hit
AGGCCGAC13122880.3215925532373734No Hit
AGGTGTAA13110540.3212901461356587No Hit
TCGGTAAT13106170.32118305383140483No Hit
CTCCTTCA13089190.32076693773844583No Hit
CATCGAGC13081600.320580935315268No Hit
GAGTGGCA13046490.31972052094401987No Hit
ATCCTAAC12923080.31669620486439226No Hit
ACATCCAG12908910.3163489513286307No Hit
CACTCTCG12807300.3138588714578669No Hit
AAGCGCAC12650650.31001996769096246No Hit
AGTTCCGG12526430.3069758015503633No Hit
GAGCCGTC12298960.3014013652920949No Hit
GGAAGGCG12255950.30034735156075393No Hit
TATTAGAG12239330.29994005771711557No Hit
TTCGTTCG12087050.29620824625446507No Hit
ACGAGTTC12004550.294186480785141No Hit
AGCGCTTC11573420.28362110203617513No Hit
CTCTTAAT11467530.2810261354234876No Hit
GTGGCGAA11203580.27455771123405626No Hit
CGCTACAA11123650.2725989268223826No Hit
ATGCAACT10803350.2647495755517827No Hit
TCGGCCAG10791880.26446848888592633No Hit
GCAACAGC10744230.263300766163341No Hit
ATGCTAGG10649060.26096850653042497No Hit
GATTGGAT10484370.2569325725286919No Hit
TAGTCGTG10475210.25670809529597666No Hit
CTCACCTT10372420.25418909805243944No Hit
TATATAGC9900440.24262263906805678No Hit
TGATAAGG9488960.23253880809451177No Hit
ACCACCAA9175950.22486810737265572No Hit
AGAGTATT8800120.21565792414433982No Hit
TAACATTA8434280.206692558334673No Hit
TGCCTAGA8288970.20313155542137024No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)