FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352040583
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTGTAC35890321.0194938235288629No Hit
ACGCTATT34331810.9752230753464012No Hit
ACACTGTG33533530.9525472806071338No Hit
TGCTTCTT30016950.8526559564298869No Hit
CGCCGGTA29210770.8297557557447858No Hit
CCATTGAC26438090.7509955180366237No Hit
CATACGTT25683770.7295684429655657No Hit
ATGGCGCT24650450.7002161452505037No Hit
TGTTGCGT24214070.687820415295699No Hit
TAGCCACC23015810.6537828623014182No Hit
TTGCGTCG22767990.6467433329980595No Hit
CAGATAGT22231630.6315075895667404No Hit
CCGAATAG22187090.6302423945252926No Hit
GCCGGTTG21946100.6233968769447243No Hit
CTCGTATC21561640.6124759769529186No Hit
GCCTACAT21398370.6078381593863No Hit
TCCGGTCT21386460.6074998461185936No Hit
CTAGTCCG21378880.607284530033857No Hit
GTTAATGG21320260.6056193811041383No Hit
AGTCTACC21109940.5996450698980919No Hit
TCAATAAC21109110.5996214930708713No Hit
TTGACCGA20948950.59507201759179No Hit
GTAATCTT20762470.589774901037475No Hit
GGCGTGTC20499280.5822987743432978No Hit
TTATCGTC20417520.5799763148329975No Hit
GTCCATCC20072730.5701822735590686No Hit
CTCGAACA20047600.5694684354047898No Hit
TGTCGGCT19978930.5675178080249913No Hit
TAGGTCTC19964990.5671218309509504No Hit
TGCTAACT19900290.5652839746603874No Hit
TCCGCGAT19531080.5547962633614887No Hit
TCAAGGTT19479680.5533362044227724No Hit
TGTATATT19463410.5528740417976186No Hit
GCAGTTAA19088310.5422190202429019No Hit
CCTGGCCA18922860.5375192779975597ABI Solid3 GAPDH Forward Primer (100% over 8bp)
TACATGTC18919520.5374244025723591No Hit
ATGTCAAC18911830.5372059618478703No Hit
CCGCTGTA18841250.5352010793596487No Hit
GATCTTCC18608150.528579683666755No Hit
GCACTCTC18457150.5242904054615771No Hit
ACTGAGGC18387170.5223025664629126No Hit
GCAGTGGT18300170.5198312604771479Clontech Universal Primer Mix Long (100% over 8bp)
CGTTCATA18158370.5158033157785107No Hit
GCAGATCT18087120.5137794013936172No Hit
AATTATTG17772420.5048400911209717No Hit
GTCTCCAC17706680.5029726927818433No Hit
CCGCGTAC17682940.5022983387117047No Hit
TACAGCCT17665470.5018020891074367No Hit
TTGTGGCT17596850.4998528820184348No Hit
GTTAAGCC17486830.4967276741499999No Hit
TCATTGCG17351010.4928695962306141No Hit
AGCTCACG17058570.48456259942053326No Hit
GATCGATT17054040.484433921074378RNA PCR Primer, Index 42 (100% over 8bp)
TGAGCTCC16834640.47820168505970234No Hit
GAAGGTGA16744270.47563465147425915No Hit
CAGCTAAC16716180.47483673210483235No Hit
ATCTCAGT16681570.4738536068155529No Hit
GCGGAGTA16671470.47356670807467677No Hit
ACCGAACT16556420.4702986189521223No Hit
GGTTCAAT16545560.4699901317911407No Hit
CTCTGAGA16522970.4693484444093198No Hit
AAGGCCTG16483880.46823806106468124No Hit
ACGGACGG16463060.46764665197705346No Hit
GCCTGGTC16438920.4669609355805435No Hit
TAACTATG16350660.46445383826670916No Hit
GTGAGTCA16227660.4609599229075246No Hit
CTCTCGAA15885930.4512528034303363No Hit
ATACCGAA15601660.44317788213639No Hit
AGGTAGCC15589790.4428407051013206No Hit
CAGAGTGC15577110.4424805193553494No Hit
TTAGCAGT15541590.4414715447735752No Hit
CAGGAATC15502900.44037252375530805No Hit
CACACATG15486400.4399038277924906ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CACAGGCA15353350.4361244339832263No Hit
GACAGTCG15283310.43413489063560606No Hit
GTATCTAG15247900.4331290406935839No Hit
AGAAGAGC15112620.4292863019147994No Hit
GTGTTAAG15027420.4268661263977057No Hit
AGATGACT14833870.42136818072477744No Hit
TCTCGAGT14827150.42117729364173906No Hit
AGCCACGC14798260.42035664961956953No Hit
CGACTCTG14725120.4182790482425715No Hit
ATGCGGCA14624240.41541346953172154No Hit
CAATCGTA14622980.41537767820365185No Hit
AGACCATC14595130.4145865762300479No Hit
GTGGTCGT14470920.41105828983359005No Hit
TTAGGATC14395040.4089028565209483No Hit
GTAGAATG14390020.40876025932498805No Hit
GAGTCCTA14354660.40775582967376234No Hit
CATTGTGA14245420.4046527783417516No Hit
ATGAATTG14177950.40273623794106717No Hit
TAAGACTG14115750.40096939619032507No Hit
AAGCTCCA14054050.3992167573475471No Hit
GTCATACA14053950.3992139167659542No Hit
CGGACATC13969000.3968008427028426No Hit
AGAACGGT13695780.3890398056749042No Hit
AACAGTGA13627690.38710565366834426No Hit
TCCTCCTA13584120.3858680122683469No Hit
GCCATCGC13487360.3831194655191217No Hit
TGATTCGC13319780.3783592188858521No Hit
GGTCGTAT13235010.3759512578696076No Hit
TACGTACC13208560.37519992403830327No Hit
ACTTGTCC13197680.3748908687610031No Hit
GTAATTCC12833590.3645485952396573No Hit
AAGCCTGT12690050.36047122442130486No Hit
GAGACTGA12614950.35833794764508725TruSeq Adapter, Index 15 (100% over 8bp)
GTCGAAGC12602550.35798571552757597No Hit
TGCTCGAG12562900.3568594249260177No Hit
CGTTGTCT12441940.3534234574313269No Hit
TACGAGCG12400840.3522559783966725No Hit
CTTGCTTG12374410.3515052126816868No Hit
TTATGACG12354060.35092715432754523No Hit
GCTTCTCA12292670.3491833212877051No Hit
CACATTAT12228400.3473576794979913No Hit
AATGCGAA12170320.3457078697088739No Hit
GTAGCACA12169800.34569309868459114No Hit
TTACCTAC12102230.3437737177023139No Hit
CAACAGCA12101950.34376576407385395No Hit
CGGAGCTG12079160.3431183955288473No Hit
CCGGCTGA11842650.336400136003638No Hit
GCTGTAGC11839100.3362992953570924No Hit
AGCGTGCG11640080.33064596987103617No Hit
CAGTACCG11620370.3300860912390888No Hit
GACGATGT11603600.3296097257059707No Hit
AATGGTCT11596990.32942196326268436No Hit
AAGTGGAG11539820.3277980027660618No Hit
GTTCTAAT11448840.3252136416329023No Hit
GCGCCTAA11433730.3247844297542252No Hit
AGAAGTAG11432900.32476085292700474No Hit
GACTAAGA11397200.3237467652983633No Hit
AACACGTA11385680.323419530298869No Hit
ACGCATTA11375500.3231303590927186No Hit
CAATACAT11323530.32165410883892326No Hit
TCGTTCTG11275830.3202991514191419No Hit
TAGCGCTG11241610.31932710439807444No Hit
CGACCTCG11204350.3182687036965849No Hit
GGCAACAC11133810.31626495744100047No Hit
CCACATAT11072230.3145157272961339No Hit
TCGTCGAC11055050.3140277153784852No Hit
GAACGCGC10972870.31169332542549505No Hit
GAACAGAC10970100.3116146413153736No Hit
CTACAAGC10892940.3094228485583436No Hit
TATAGGTG10866500.30867179878519857RNA PCR Primer, Index 39 (100% over 8bp)
CGTACACT10842350.3079857983305294No Hit
ACATACTC10810710.3070870383145571No Hit
TCGTGTCA10786370.30639564075486153No Hit
TGGAAGAT10671070.30312045017832506No Hit
GATCGGCG10506780.29845365867945967No Hit
TGCTCAGC10459950.297123414319536No Hit
CGTATGAC10414000.29581816707762926No Hit
GGAACCTG10406320.2956000104112997No Hit
AGGCGGTT10334830.29356927863058335No Hit
TCCGTATG10211550.2900674096429388No Hit
ATACTTAG10201700.2897876123560448No Hit
ATTGCCAG10146910.2882312577013315No Hit
CGAATCGT9989080.2837479677733632No Hit
GTCCGACT9982820.2835701473656519No Hit
CTGTCATT9934310.2821921812349686No Hit
TGAGTAAG9840510.2795277157008912No Hit
GCGCAATC9826710.2791357154410803No Hit
ACCATAAG9810540.27867639339751915No Hit
GATACGAG9660660.2744189297061811No Hit
CTCCAATG9633580.2736497002108419No Hit
GGATATGC9602970.27278019818527566No Hit
CGTGTTAG9523420.2705205155281771No Hit
CTAGCGGC9333010.2651117641172637No Hit
AGGATCTT9327360.2649512712572687No Hit
TGCAATCG9118810.2590272383454154No Hit
AATATCAA8920290.25338811576732334No Hit
TCCGGCGA8851810.2514428854925513No Hit
TATACTAT8751570.24859548650389549No Hit
AACTTGAA8717810.24763650615815505No Hit
GCATAACG8577880.24366168033530386No Hit
CTGTAAGG8566950.2433512047672072No Hit
AGTCATGA8527590.24223315185226812No Hit
CATTCCAG8406080.23878156115881674No Hit
AATTCATT8397080.23852590881546176No Hit
TAGCATGG8389670.23831542171943282No Hit
GAGCTTAG8026120.22798848733868843No Hit
GAAGGCAG8006230.22742349565987394No Hit
AGAACACA7915310.22484083887567022No Hit
CTTCTAGA7548620.21442471023291085No Hit
CAAGAGAG7473070.2122786508395255No Hit
AACGTCGA7415130.21063281786463808No Hit
CGATGGCA7179310.20393415835241926No Hit
ATATTCGG7140430.2028297402291258No Hit
GCTGCATG7055850.20042717631790766No Hit
TGGCGCGA6852730.19465738698654525No Hit
GCGACAAG6513810.18503008785211564No Hit
CAATCAGC4644080.13191888163643906No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)