FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001836108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339917858
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCCT38595691.1354416689693307No Hit
GCGGCTCC32346070.9515848973136327No Hit
TGGCCGCA31313540.9212090292708305No Hit
ATTACGAC28356880.8342274267920339No Hit
CGGTTGTT27077250.7965821554453311No Hit
ACCAGGCC24492820.7205511397403546No Hit
AACCGTTC24284680.7144278956947299No Hit
GTCACAAT23563090.6931995317527566No Hit
CGGTATAT23356540.6871230637138224No Hit
GCGTCGCT23057950.6783388826838277No Hit
TTAAGTGG22900710.673713059229739No Hit
CCTTGTAG22540840.6631260897154747No Hit
CTATCGCT21983190.6467206556708768No Hit
ACAGTGCC21725870.6391505914937837No Hit
GGTTAATG21241740.6249080329283553No Hit
GTGATGTC21094810.620585518045951No Hit
CGTAGTTC21047180.6191842971662878No Hit
CTCATAGG20239510.5954235567111628No Hit
TCAACTTA20191210.594002625187171No Hit
TCTTAGTA20186310.5938584727137225No Hit
CGAGTTCT20110720.5916347001692391No Hit
ACAAGTGT19736590.5806282175383678No Hit
TTAGGTCA19539810.5748391718801664No Hit
CGCGGTCG19329700.568657972656441No Hit
TCAATCGG19282740.5672764624211064No Hit
CTGTGTTG19029570.5598284865633626No Hit
GGACGTTG18976080.5582548710930039No Hit
CGGCTTGA18779720.552478181361098No Hit
ACTGTCAT18774450.5523231439049607No Hit
CGGTCGGA18647750.5485957728057935No Hit
AGTGGCAA18484630.5437969663835667No Hit
ACGATTCT18164690.5343846924335467No Hit
CCTGTGCG18086580.5320867843312899No Hit
ACTATTAC18012490.529907140095005No Hit
CCATGCGG17988110.5291899079924185No Hit
GCTAGTCT17828450.5244928908677696No Hit
GCATCGAA17811070.5239815908701095No Hit
TGCGGATT17781880.5231228539925666No Hit
GTGTAGAT17728990.5215668898454874Illumina Single End PCR Primer 1 (100% over 8bp)
TTCGACAA17463440.5137547083507451No Hit
ACTGGTTA17456890.5135620147382783No Hit
CCTATACC17257050.507682947331352No Hit
CATGACTA17160160.5048325528104499No Hit
TTCCTTAT17085350.5026317269862297No Hit
AGTGCTCA16964530.49907733885520067No Hit
ATAGCCTA16676800.4906126467765633No Hit
ACACGAAT16629430.48921907480365445No Hit
TATGCCGC16501170.48544581026396083No Hit
CATGTATT16388730.4821379522814009No Hit
ATCTAACC16262840.478434410468661No Hit
TCTCTCAG16164260.47553429805385516ABI Solid3 Adapter A (100% over 8bp)
GTTCACTG16149450.4750986045575752No Hit
CTGCGCTT16133030.4746155466771622No Hit
CTTCTGCT16126500.4744234414421381Illumina Single End Adapter 1 (100% over 8bp)
TCTGTGTT16044730.47201786026787684No Hit
ACCTATAA16022260.47135681821106323No Hit
TTATACGT15980360.4701241674687183No Hit
CAAGGTCC15960730.46954667500876046No Hit
CACCACGG15822440.4654783391815796No Hit
GCCAACTG15787960.4644639764704566No Hit
ACGCCTCG15710010.4621707753877409No Hit
GGCTACGG15690270.4615900468518485No Hit
GTAGTGAC15666150.4608804636560166RNA PCR Primer, Index 43 (100% over 8bp)
GCGCTCAT15634300.4599434725786016No Hit
GACGCAAG15557930.457696753313855No Hit
GCACACCA15528230.4568230128115246No Hit
ACGATAGA15464570.45495020741158No Hit
CCTGGTGC15308690.45036439362359126No Hit
CCTTGGTT15303420.4502093561674538No Hit
TGGTTAGT15255220.4487913665306752No Hit
CGACGCCT15065620.44321354837438404No Hit
CTATTGGA14931950.43928112773645456No Hit
GTGCCACG14893760.4381576210097205No Hit
GTGTGTGT14701530.4325024312197214No Hit
AATCAGCT14487680.426211205414221No Hit
TTGACTCT14442970.4248958876411842No Hit
CATTCTTC14432690.42459346163566375No Hit
CATCAGTG14384510.42317606037632777No Hit
GCTACCTC14215480.41820338841979876No Hit
GTAAGAGA14208480.4179974563148724No Hit
CCAGGATA14095150.41466341553611463No Hit
CCATAAGA14071270.4139608928695944No Hit
CTGCCTTA14054790.4134760698568535No Hit
CACGCCGT13827170.40677974618209084No Hit
AACGCGCC13802950.40606722109904564No Hit
TGGTTCCA13532940.3981238314345933No Hit
TCCAGATC13456520.39587564122624No Hit
TATGAGGT13283170.3907758797420994No Hit
CCACCAGG13260470.3901080713446953No Hit
ATGAGACG13140130.3865678042722898No Hit
GAGAATTC13062410.3842813695301645Illumina Small RNA Adapter 2 (100% over 8bp)
CCGTTACT13034710.38346646677209884No Hit
TAGGTGAA13008460.38269422137862497No Hit
ACTTAAGG12743690.374904986604146No Hit
CACTTGCT12648090.37209254242829454No Hit
GCACGTCC12403620.36490051075810204No Hit
GGTCTCGC12313140.36223869120756813No Hit
CGATCTAA12271190.3610045695216166No Hit
CACGATAC12234340.35992048408353994No Hit
TAAGTCCT12219930.359496558136113No Hit
TATTGACC12133300.3569480012432886No Hit
ACAACCTT12046890.35440591650233333No Hit
TACAAGAA12025950.3537898853198822No Hit
TTCCGAGC12009990.3533203601206501No Hit
CCAAGACG11969270.3521224236474213No Hit
GACAAGGC11933910.3510821723288219No Hit
CGTATCCA11891610.34983775403762396No Hit
AGTCTGAT11816260.34762104202245236No Hit
TTGCAGAA11792930.346934699735605No Hit
AACGGCCG11745000.34552465319430203No Hit
TCTCTGCC11724440.34491980118326115No Hit
GAGAAGCT11698000.34414196620408216No Hit
CTTCGACG11581000.3406999581645987No Hit
CTCTAGTT11578050.34061317249180834No Hit
TCATCATG11565060.34023102134280925No Hit
AAGGTGTT11528460.3391542906227657No Hit
TGCTATAC11438200.3364989432241009No Hit
GCTGACAA11350630.33392273259147215No Hit
CAGCACAA11337820.33354587683945686No Hit
GCAAGCAC11210050.3297870275471082No Hit
ACCGACAC11190860.3292224793908886No Hit
ATGGAGAG11123160.3272308217475294No Hit
AATATTCG11106900.32675247088665754No Hit
GAGCGTTA11051510.32512295955924736No Hit
TTCAGTAA11023010.3242845217034758No Hit
GAGTTCCT11012600.3239782712445782RNA PCR Primer, Index 1 (100% over 8bp)
ATTAGGCT10945160.3219942625079733No Hit
TGACGACC10913670.32106786222452605No Hit
ATGTAATA10811820.3180715500978475No Hit
AATCCAAG10705860.31495432640670495No Hit
AGCGTTAT10699350.31476280954912345No Hit
AGACTCAA10671610.31394673003617246No Hit
GATTGAGG10638620.31297620144452665No Hit
ATACATGT10598540.31179709304946257No Hit
AAGCATCC10532380.3098507404691871No Hit
CAACTCGA10508320.3091429224056831No Hit
TGAGATAA10335410.30405610522528065No Hit
AGCAACTA10278270.3023751108716389No Hit
GACATCTA10276900.3023348070168176No Hit
TCGGAGCT10276480.30232245109052197No Hit
CACACCAC10238200.3011962966652961No Hit
TCTGATAT10183280.29958061220778814No Hit
TTGATGAG10015510.2946450080301459No Hit
GTCCGCGG9868830.29032984786577465No Hit
CCTCCATC9815560.28876270454728503No Hit
ATTCCACA9764450.28725910599260135No Hit
TACCTCGC9747310.286754866524253No Hit
ATTAGTGC9697630.28529333695671855No Hit
AGAGATCG9679210.2847514413320409No Hit
AATGTAGG9640310.2836070472060929No Hit
AGGACCAG9621770.2830616213167594No Hit
AGGTCTTG9562100.28130619721662287No Hit
AGAATGTA9533770.28047276056911374No Hit
TGCCGTAG9526250.28025153065067854No Hit
CTCAATTC9495640.2793510189747077No Hit
GCGATCCA9484750.27903064745718653No Hit
GCCACTGT9393930.2763588254901277No Hit
CTAGGCAC9388200.2761902553528094No Hit
AACAGAAT9332870.2745625091577272No Hit
CTGATTAC9211490.27099164645830404No Hit
CATATCTC9038420.2659001222583604No Hit
CGACTTAC8958910.2635610277351183No Hit
CGCCAGCC8922720.262496358752649No Hit
CACCGGAG8878470.2611945736607931No Hit
TATACGGA8868690.2609068570913388No Hit
ACGACGAT8842050.2601231383377334No Hit
GCTTATAC8827920.2597074496745034No Hit
AGCAGGAA8625590.2537551292759676Illumina Paired End Adapter 2 (100% over 8bp)
TGCGCCTG8300390.2441881120585315No Hit
GATGGTAC8226850.2420246482019194No Hit
AACAATAG8144430.2395999447607722No Hit
TGTAGCAC8043830.23664040622425903No Hit
TATCACAT8043770.2366386410919311No Hit
AAGATGGC8033540.2363376860300173No Hit
ATACGTTA7951470.23392327919411637No Hit
GTCGACCG7935880.23346463897757322No Hit
TTCTATGA7923950.23311367183303447No Hit
CCTAACCT7850440.2309510905425863No Hit
TGTGTCGA7788300.22912300182828288No Hit
TGAATTGA7006330.20611832638695904No Hit
TTGCTCTA6773940.1992816746921252No Hit
TCTCATTC6759020.19884274511991074No Hit
AAGAACGT6661620.19597734697422106No Hit
GTATATCA6397960.18822076714780897No Hit
TGGCACAG6305080.1854883423041575No Hit
TCCACAGG6136220.180520671555891No Hit
AGTAACCG6040390.17770146103944912No Hit
TACCTGCA5865760.17256404339897904No Hit
TATGCTTA5592700.16453092617452303No Hit
CTTCGCAA5227810.15379627392215445No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)