FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349820895
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATCTC35666151.019554592357898Illumina PCR Primer Index 7 (100% over 8bp)
GGCCGCTC33953330.9705918224238721No Hit
CGTGTGGT32605320.9320575318978588No Hit
ATTGACCT30340300.8673095413583No Hit
GAGTGAAC29335860.8385965623923065No Hit
GCGAGATA29251990.8361990498023282No Hit
TGTCCTGC29124590.8325571861566473No Hit
CACTGCTT27517200.786608244198792Clontech Universal Primer Mix Long (100% over 8bp)
GTTCAGGA26921400.7695766715135754No Hit
GACGCCTC26886480.7685784464075538No Hit
GTGTCGGC26716480.763718816739063No Hit
TAACCTCT26605210.7605380461907514No Hit
AGTGTCTC26044090.744497837957907No Hit
TAGGATCA25742070.73586427706098No Hit
GTGCGGTG24849150.7103392151575165No Hit
TCACCGAG24288110.694301293809222No Hit
TTCTTGCC24263580.6936000778341157No Hit
CAGGCGCA23925400.6839328451206438No Hit
GAGTATAA23619670.6751932299527162No Hit
TCACGCCG23382260.6684066141903845No Hit
CGCACCGA22193450.6344232239186285No Hit
GCACCACT22187950.6342660006058243No Hit
TCTGACCG22032620.6298257283916674No Hit
CGCCTCAT21659660.6191642726201361No Hit
ATATACAA21595130.6173196143700908No Hit
CTGACTGG21556300.6162096177816937No Hit
GATGGCGT21500030.6146010803614232No Hit
GCATCCGT21497970.6145421930842639No Hit
TCCGACTT21450830.6131946463632483No Hit
CTATCCTG21299840.6088784376359222No Hit
GGTGTACG20974980.5995919712000051No Hit
TGATCCAT20697180.5916507646005537No Hit
GGTAATTA20350600.5817434090093446No Hit
CTTATGTA20262940.5792375552638158No Hit
GGATTGTG20122020.5752092081292057No Hit
GGCTCTTA19926910.5696317825726219No Hit
GAACAAGT19843390.5672442751025493No Hit
TACTGTTG19756570.5647624336447941No Hit
GGTCCGTG19577840.5596532476997979No Hit
ACACTAGC19453360.5560948553401877No Hit
TGGCTGTG19277300.5510619941670436No Hit
ATTCTGGC18805430.537573091510157No Hit
CCACTACA18740890.5357281473995428No Hit
AGCTTCAC18504100.5289592549924726No Hit
CTGAACTA18403480.5260829259498636No Hit
TGCAGTGT18204430.5203928713291983No Hit
GATTACGC18189320.5199609360098402No Hit
AGGTTATC18042040.5157507815535147No Hit
CGTGATCC17915000.5121192088883084No Hit
TCCTTACA17827540.5096190723541543No Hit
AATTAACA17687920.5056278870934796No Hit
TTACAATT17512540.5006144644390096No Hit
ATCCTCCG17321800.49516195995096296No Hit
ATAATATG17235090.49268326295946385No Hit
CCGGTAGG16841850.48144208195453847No Hit
TTAAGGAC16829800.4810976199692131No Hit
TTAATACT16741880.4785843338488972No Hit
TTCCGGTT16643180.4757628900354851No Hit
GTAGCTTC16363070.46775564964465605No Hit
GCCATGCG16233460.4640506108132849No Hit
GGCACTCC16223630.46376960987421867No Hit
TCTAGTGA16082000.4597209666392283No Hit
AGAGCCGC16063830.4592015579858373No Hit
TTACAGCG16024580.4580795552535534No Hit
GGCGTTGA15921140.45512261353056116No Hit
TAGGCATT15853760.45319648501842635No Hit
ATCGCGTG15646570.447273739894811No Hit
ATAACTGA15542260.4442919283023389No Hit
CGGCACTC15500220.44309017047137794No Hit
CGTCGTGG15390760.4399611406860074No Hit
TCGATTGC15347230.438716789630305No Hit
AACCAACG15332910.4383074372958768No Hit
ACTCTTGT15293070.43716856879003757No Hit
GACCACTT15234560.4354959986023705ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GACTTATC15231520.4354090969894751No Hit
CCGAAGGT15229260.4353444925009411No Hit
GCGTACGA15144290.4329155352484019No Hit
CAGTGGTC14991940.42856044948372796No Hit
CTTGCCGA14760340.42193991871183106No Hit
CCAGCGTG14758860.42189761134765835No Hit
GGAATATC14640010.41850015848824584RNA PCR Primer, Index 37 (100% over 8bp)
AGATGGAC14584780.4169213505671238RNA PCR Primer, Index 16 (100% over 8bp)
GTTGCAAC14579170.41676098278806356No Hit
GGCCTAAG14563130.4163024624358131No Hit
GAGGTATA14217280.40641597466612167No Hit
TACTCCGG14137640.40413938109671804No Hit
AATCGCGG14066080.4020937628668522No Hit
AAGTGAGA14016290.4006704630951219No Hit
TGAGCGTA14009060.4004637859039266No Hit
TTCACGGT13999500.4001905032002162No Hit
TACACACA13902370.39741393949609555No Hit
CTAATGAT13749420.39304170209729755No Hit
CGCTTACC13354210.38174420656033137No Hit
ATAGGTAT13324880.38090577751223237No Hit
GGACACAT13254610.37889703529573326No Hit
TTAGAGAT13007840.37184285404106576No Hit
TAACGGTC12979360.37102872314130925No Hit
GCAGCTGG12802810.36598185480029716No Hit
TAATAGGC12755330.3646245888199446No Hit
TGACGGAA12433280.3554184492038419No Hit
TCGGCAGC12415840.35491990837196846No Hit
GGCTGAGT12371010.35363839544233056No Hit
ACTCCGTT12353890.3531490021486567No Hit
GTGGTACC12206640.34893970527403745No Hit
TACTGCAC12008710.3432816670370705No Hit
CTGGATGC11852230.3388085208575091No Hit
GACTCGGT11753140.33597592848191643No Hit
GTTATCCG11749790.3358801651913903No Hit
GTACTATA11709520.33472900468109545No Hit
CGGAGAGA11542730.32996113625516854No Hit
GTTACCAA11458290.3275473296127723No Hit
ACCTAGGT11420180.32645791498532417No Hit
ACGTGGTA11379700.3253007514030859No Hit
ATGTGCAT11351060.32448204673422953No Hit
TGTGAGTC11283760.3225582051066446No Hit
GACCTGTG11240820.3213307198244976No Hit
GGAAGAAT11207200.3203696565924114No Hit
TCTCCACG11161500.3190632737932936No Hit
CCATCTCC10916540.31206083330156703No Hit
CGAGCCTT10721250.3064782622547461No Hit
TCGGATTG10641530.3041993818007927No Hit
GCATGTAT10617460.3035113154118481No Hit
GTTGCCTT10513450.30053807963643797No Hit
AATCTGTA10418880.2978346962379134No Hit
AACGGATA10395240.29715892185342446No Hit
AGGCCAGA10350380.29587655134208035No Hit
TGTGAACA10341810.2956315688346747No Hit
ACCTCGCA10293520.29425114814825454No Hit
TCTATGAA10268000.29352163197684344No Hit
CGAGGAGT10201960.29163380878091916No Hit
TTAACCAG10186560.29119358350506763No Hit
GATATTGT10125880.28945898157398514No Hit
GGAGTGCA10061660.2876231850015706No Hit
ACAGCGGA10054840.2874282280936935No Hit
GGTAGCGA10026770.2866258174772551No Hit
TAGATCCG10023640.28653634311924103No Hit
TTATGCTA9947160.2843500814895577No Hit
GCGGAAGT9900350.28301196816731033No Hit
ACAGGCTG9878300.2823816456132502No Hit
CGCGTCGG9828820.2809672075191506No Hit
GGCCGTCA9791830.27990980927540077No Hit
GGACCGGC9708390.277524588689878No Hit
CTTACAAG9676620.2766164096630077No Hit
TCGCAACA9579130.2738295549784126No Hit
CCTACTCG9553600.27309975294643274No Hit
CGAGAGGA9493170.27137229752956865No Hit
ATAGTTGC9358070.2675103212459622No Hit
AGCAAGCT9326410.26660528668534794No Hit
TATCAAGA9269210.26497016423218517No Hit
CGATGCTC9149880.2615589900654734No Hit
ATTCGATC9078130.2595079404848015No Hit
TACTCAAT9047430.2586303485387858No Hit
GCTGGAAT8899250.25439446663127424No Hit
GTCTTGTA8814550.25197322761409097No Hit
CTTAACAC8811990.25190004730849486No Hit
CGCGATTA8560400.24470808125969717No Hit
GGCGGAAC8541250.24416065827057015No Hit
CGTAATGT8527560.24376931515197228No Hit
CATCTCCG8523660.24365782953016574No Hit
GCGCGAGG8515570.2434265683300593No Hit
CAGGCTAG8365240.2391292264002698No Hit
AGTTACAT8347610.23862525421759043No Hit
ATTAATCA8193400.2342169983871318No Hit
ATAAGCCA8145380.2328442959360675No Hit
TGTCTTAA8129150.23238034423301102No Hit
ATGGTCAA8056150.23029356208124735No Hit
ATCGGCGC8029610.22953488813182527No Hit
TAATTAGA7960050.22754644201570637No Hit
TGTGGAGC7764970.2219698740408288No Hit
TCATTCAA7694280.21994912568044284No Hit
CGCAAGTG7582170.21674434284435756No Hit
TCCAACCA7360830.21041710501598251No Hit
CTTGCGAT7356080.21028132124583354No Hit
AGTGGATG7247270.2071708723974307No Hit
ATCGCTAA7054920.20167234435781775No Hit
CCGACACA7035570.201119204157316No Hit
AAGCAGGA6819830.19495204824743245No Hit
GATACACC6768850.19349473106802267No Hit
TGGAGTTA6714760.1919485112517364No Hit
CAAGACGC6364970.1819493944179635No Hit
AGCGAAGA6224000.1779196179805097No Hit
CGGATGCG6112060.1747196947740929No Hit
ACTCATAG5861830.1675666057626432No Hit
GGTTGGCC5429260.15520113514088402No Hit
TCGTGAAG5376460.15369179133796454No Hit
TCGCACGC5369060.15348025451710082No Hit
GCCTTAGG4962740.14186516788827036No Hit
TTCGCATA4806060.13738630449733427No Hit
ACTACATA4531020.12952399541485365No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)