FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348480467
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCGAT32291470.9266364418640429No Hit
AACCTTCT30598090.8780431874249067No Hit
CTCTCTGC25882010.7427104945884958No Hit
CTGTTCGC25764190.739329530340649No Hit
CCGGTCTT24441040.7013604007825208No Hit
CAATTAAG24189580.6941445013616789No Hit
CCAACAAT24036270.6897451156136105RNA PCR Primer, Index 36 (100% over 8bp)
GCCGTCCT23567850.6763033292193102No Hit
TGGCTTCC22956630.6587637521732316No Hit
TGGCCTAT22907740.6573608040992438No Hit
CCGTACAC22141390.6353696145614957No Hit
GGCGCCAT21965290.6303162466778949No Hit
CGACAGTT21536490.6180113963173723No Hit
GGAGAACC21091270.6052353574239212No Hit
TCGGTAAT20883850.5992832304141741No Hit
TTGAGAGT20288710.582205085256615No Hit
CGAACGAG20224930.5803748535495391No Hit
CATTAAGT20218830.5801998078704365No Hit
CAACCGGT20212730.580024762191334No Hit
TCGTCTGG20050340.5753648166455194No Hit
CCTTACTG20041610.575114300452312No Hit
ATATGGTT19867110.5701068461894595No Hit
TCTACCGT19503930.5596850281998732No Hit
GTCCAGAG19222540.55161025711091No Hit
GTATGCCT19181350.550428268336773No Hit
GTTCGGAC18731830.5375288365875611No Hit
GTTGTGTG18585760.5333372099733785No Hit
CAAGCACT18405380.5281610231542763No Hit
GTCTGGCG18365000.527002278150643No Hit
ACGAGTTC18274120.5243943844921443No Hit
CTCACGCC18002640.5166039908916903No Hit
GGTGAGAT17812520.5111483049062833No Hit
GAGACCAT17532670.5031177256772903No Hit
GCTCACGT17499150.5021558353226151No Hit
TTGTACTC17319670.49700547491518365No Hit
CGTTCGCG17293510.4962547872159503No Hit
CGTCCGAA17238160.49466646289819166No Hit
AAGACACT17152920.49222041475283035No Hit
TCTGCGCA17102310.49076810953653827No Hit
TCCTGGAA16947290.4863196536062952No Hit
TAGCTGGT16945390.4862651311816567No Hit
GCCTGTGA16898140.48490924456893586No Hit
TATCTACT16882830.4844699086104014No Hit
CTCTATAG16878130.48433503734945355No Hit
GAGCCGTC16849760.48352093146156166No Hit
TAATGATT16785080.48166487334281494No Hit
TATAACGG16783420.4816172379612887No Hit
AGCTGTGG16675520.47852093816208063No Hit
ATATTACA16482850.4729920773436062No Hit
CTTCCTGT16421910.4712433423133584No Hit
ATTATCGT16361440.46950809440920543No Hit
CTATGGAG16335140.4687533892681566No Hit
TCGGCCAG16136410.46305063061109825No Hit
CAGCGTCT16132690.4629438814428586No Hit
TTCACTTG16092690.4617960409241531No Hit
AGCACAAC16087250.4616399346136092No Hit
GATTATCT16015820.4595901784073309No Hit
ATCTGTCT15900920.4562930065173495No Hit
CTGATCCT15892390.45604822952673557No Hit
CCAGATTC15844620.45467742098727154No Hit
TATAATCC15810470.4536974521444268No Hit
ACTCGGAA15740090.4516778267517645No Hit
GATATATG15725280.4512528387997139No Hit
TCAGGTAC15679930.4499514746116315No Hit
ACGTGCGC15651950.4491485601687971No Hit
ATCATGGA15622060.44829083634119443No Hit
TCGAGCAA15461840.44369316114351964No Hit
GACGCTCA15414240.44232723092626025No Hit
TGTAGACG15388870.44159921307727124No Hit
GTCAACGT15388280.4415822824296204No Hit
ATGCTAGG15317210.43954285678801047No Hit
ACGCGACC15257860.4378397484183812No Hit
CAATATGG15243370.4374239431904802No Hit
ACTTCTAT15218830.43671974303225436No Hit
AACTACCT15212790.43654641911392983No Hit
CTGGCCTC15177210.4355254149725414No Hit
CCGACCGC15082070.4327952762988004No Hit
CTCACCTT14934400.42855773606386954No Hit
CATCGAGC14800350.42471103552555783No Hit
CTAACATA14638700.42007232502933944No Hit
TCTCTATA14620910.4195618229586452No Hit
TTCGGAAG14554930.41766846002304053No Hit
ACAGCACG14430970.4141113022555723No Hit
AGACAGAG14350130.41179151656726865No Hit
ATAGGAGG14283270.40987290114025243No Hit
AGGTGTAA14252840.40899968146564725No Hit
GCAACAGC14249860.4089141673470037No Hit
TGTACGCC14232880.4084269090468133No Hit
TGCCAGGT14168850.40658950333649546No Hit
TATTAGAG14067180.40367197969807583No Hit
ATGCAACT14013160.40212182107756417No Hit
GGCACGTT13964840.400735229730968No Hit
CGCGCGCT13959780.4005900279053517No Hit
TTCGTTCG13948090.4002545715137601No Hit
CCAATCTA13935130.3998826711856995No Hit
GCTCCAGA13895750.398752622195034No Hit
ACCTGAAC13766550.39504509731961535No Hit
TGTACCTA13724950.39385134318016163No Hit
ACTAAGTG13715010.39356610481126336No Hit
CGCGTGAA13712150.3934840342141759No Hit
TTGGAACG13673030.39236144618688196No Hit
ATGTTGTG13669660.39226474062318106No Hit
GCGCTGGC13359960.38337758540710404No Hit
GAGAGCGG13325040.38237552063427416No Hit
TACGTCAG13128480.37673503232535555No Hit
ACCAGCGG13016510.37352193975336934No Hit
GAGTAATT12988110.3727069729850884No Hit
CAGAGGAT12863300.3691254236065977No Hit
TAAGCAAC12749970.3658733044569755No Hit
ATAACGCG12707770.36466233270974124No Hit
TGAGGCGG12687350.3640763601249421No Hit
GTCCTCAA12679700.36385683562573967No Hit
GGTTGCAG12678270.36381580032719596No Hit
TGATAAGG12665890.36346054368665665No Hit
AACTAGTC12643040.3628048397903461No Hit
CACGGCAA12579300.360975755923789No Hit
TGCTGCCG12559710.36041360102975295No Hit
CTGTAGCA12507700.3589211213953062No Hit
TCGCGGAT12495180.3585618473129514No Hit
TAATTGAT12424810.35654250888041883No Hit
GGATACTA12345120.3542557236070279No Hit
GGATCAGA12329030.35379400475837863No Hit
CTACGGCC12193830.34991430380515415No Hit
GTCAGATG12159600.348932039281272No Hit
AGTTCGTC11986370.34396102895488834No Hit
GGAAGGCG11974910.3436321726462792No Hit
TGCATCAA11862760.34041391479195876No Hit
AATTGCTA11844790.3398982474389303No Hit
ATCCTAAC11811830.33895242685151705No Hit
CCGTGGCG11759980.3374645385791451No Hit
CTCTTAAT11603490.33297389950983963No Hit
TATATAGC11503240.3300971242098341No Hit
TAGAGTAG11487340.3296408576036487No Hit
AGCATAGT11428410.3279498015594659No Hit
GGCCAATA11418940.32767805031666236No Hit
AGGAATAC11360230.32599330739533244No Hit
TGCCTGAC11169070.32050777755643906No Hit
CACTCTCG11168760.3204988817924191No Hit
CGAACTGC11138330.3196256621178139No Hit
GATTGGAT11117850.3190379677722367No Hit
GTTGGTCG11005330.3158090923931183No Hit
GGCCTGCT10949820.3142161767132848No Hit
AGCCATTG10938870.3139019553712891No Hit
CTCCTTCA10901750.3128367593699305No Hit
ACCGTTCA10838200.31101312774583717No Hit
AGCGCTTC10744200.30831570252687934No Hit
GGTGCGGA10712920.3074180912412517No Hit
GTCATTAG10653840.30572273079512374No Hit
CCAGCCAA10649520.3055987640191036No Hit
CATCCTCA10475320.3005999185601413No Hit
TTGTCACA10455140.3000208330184544No Hit
GCCACGAC10284510.29512443232578656No Hit
TAGTCGTG10227700.29349421182909513No Hit
CTGCTCAG10128330.2906426890205011No Hit
CGCTACAA10108630.2900773775650387No Hit
CGCATATA10082460.28932640290567563No Hit
AAGCGCAC10079930.2892538019928675No Hit
GAGGTGCG10031030.2878505669587501No Hit
AGGCCGAC9998040.2869038854909478No Hit
GCCTCATT9919390.2846469440710432No Hit
CAGATTCA9886400.2837002626032408No Hit
GAGTGGCA9882320.28358318287033285No Hit
GGTCAACT9870690.28324944823951925No Hit
TAACATTA9562640.27440964144483887No Hit
CGTGCCAC9552470.274117802992958No Hit
GGATCTCT9502610.27268701978639165No Hit
ATTGAGTA9334560.26786465480718036No Hit
AACTCTAC9271600.26605795383073794No Hit
CCTTAGCC9203650.26410805974958707No Hit
AACGAGAT9129300.2619745111854433No Hit
CTCAGAAC8995580.2581372803314109No Hit
AAGTTACG8882150.2548822915804919No Hit
ACCACCAA8857260.2541680478177275No Hit
TGCCTAGA8690070.24937036140966837No Hit
CATAATAA8500390.24392730166996707No Hit
GATGTCCA8103550.23253957588389018No Hit
CGCTGAAG8014220.22997616104549126No Hit
GTGGCGAA7977020.22890866936309517No Hit
AGTTCCGG7889230.22638944638466635No Hit
CGAAGCAA7882050.22618340901155876No Hit
AGGCGTGC7791900.22359646344252632No Hit
CAACGTAA7750610.22241160506709262No Hit
AGAGTATT7580320.21752496101883378No Hit
ACATTCCT7455340.21393853331813859TruSeq Adapter, Index 27 (100% over 8bp)
AACGCTGG7155650.20533862519186763No Hit
CTTAGATT7001750.20092230879614842No Hit
AGATGCGA6847480.1964953748756311No Hit
CGGCCAAG6337210.18185266033863529No Hit
ACATCCAG5338100.15318218682254003No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)