FastQCFastQC Report
Wed 20 Sep 2023
EGAF00001836114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338745944
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGCT56451751.6664922783547778No Hit
GTAATCTT48049341.4184476847935337No Hit
TGTCGGCT43664311.2889987547718063No Hit
AACAGTGA38768941.1444842569096563No Hit
CCATTGAC35126931.0369697592600546No Hit
TGTATATT32805330.9684346213160857No Hit
GTCATACA32537620.9605316484615976No Hit
GGCGTGTC31709860.9360956363214786No Hit
CATACGTT31522980.9305788174986975No Hit
GGATATGC31278090.923349505846777No Hit
GCACTCTC31084960.9176481829698306No Hit
CTCTGAGA30265470.8934563065941832No Hit
CGCCGGTA29601640.8738596143899512No Hit
GCCTACAT29584850.8733639626988419No Hit
TGCTCAGC29360010.8667265400526832No Hit
GTCCATCC27894720.8234702287682595No Hit
TTGTGGCT26694350.7880345277285445No Hit
TCCGGCGA26028000.7683634434896732No Hit
TTGCGTCG25814960.7620743644977783No Hit
GTAGCACA24727480.729971249486016No Hit
ACTTGTCC24618160.7267440521738026No Hit
TAGGTCTC24071760.7106139697424687No Hit
ACACTGTG23181780.684341182842325No Hit
CACAGGCA22744360.671428260702658No Hit
ACCATAAG22363620.6601885689294039No Hit
CTCTCGAA22041990.6506938426988221No Hit
GCTTCTCA21447270.6331373225239267No Hit
TAGCCACC20660920.6099237604450845No Hit
CAGAGTGC20507690.6054003114499283No Hit
ACTGAGGC20220010.5969078112415717No Hit
ACCGAACT20169460.5954155424514839No Hit
ATGCGGCA20120980.5939843814041357No Hit
CAGATAGT19818060.5850419865101028No Hit
AGAAGTAG19729030.582413763159331No Hit
GGAACCTG19724220.5822717688392455No Hit
TACGAGCG19597660.578535635544023No Hit
GCAGTTAA19474330.5748948539439929No Hit
TTGTGTAC19205450.5669573419305649No Hit
TTACCTAC18977140.5602174826335338No Hit
CAATCGTA18888050.5575874880438421No Hit
AAGGCCTG18845230.5563234138679458No Hit
CTCGTATC18708230.5522790850006458No Hit
AGAACACA18645930.5504399485887276No Hit
TCCGTATG18327630.5410435261182049No Hit
GTTAATGG18307550.5404507514929832No Hit
TCCTCCTA18126210.5350974770638139No Hit
ACGCTATT17992290.5311440717944065No Hit
GTTAAGCC17964640.5303278258587798No Hit
CGTTCATA17946540.5297935021179175No Hit
GAGTCCTA17843770.5267596650544693No Hit
GCGCCTAA17729730.5233931302805502No Hit
AATTCATT17671450.5216726668762711No Hit
TACAGCCT17644220.5208688196130845No Hit
CGGACATC17575840.5188501976572744No Hit
AGGTAGCC17243860.509049932712995No Hit
TGATTCGC17189560.5074469614904084No Hit
TAACTATG17020090.5024440971609095No Hit
CGTTGTCT17008070.5020892589639391No Hit
ATGAATTG16969880.5009618653913683No Hit
TGCTAACT16855390.49758204632554953No Hit
TCCGCGAT16470420.48621748220843647No Hit
GTCTCCAC16401190.4841737677012599No Hit
AGCTCACG16368700.4832146418260878No Hit
GACGATGT16363510.4830614296595091No Hit
AATGCGAA16310650.48150096817100196No Hit
AGGCGGTT16221480.47886861192941693No Hit
CAAGAGAG16180540.47766003657301354No Hit
TTAGGATC16091460.4750303371898085No Hit
TCCGGTCT15782970.4659235122827035No Hit
AGACCATC15676250.4627730686570228No Hit
CCGAATAG15623220.4612075886582423No Hit
ATACCGAA15611000.4608468463315386No Hit
GAAGGTGA15598570.46047990466861505No Hit
AGAAGAGC15534740.4585956016642372No Hit
TGCTTCTT15496260.4574596471035532No Hit
GCAGATCT15470670.4567042137041794No Hit
TTATGACG15408560.45487068621550786No Hit
GCCTGGTC15156370.44742587382832255No Hit
GCATAACG14960660.4416483876778167No Hit
GGCAACAC14940030.44103937669582843No Hit
AAGCTCCA14782250.4363816087492401No Hit
GCTGCATG14581410.4304526816710756No Hit
TGAGCTCC14564750.429960867664293No Hit
TTGACCGA14387610.42473157995952265No Hit
CTAGTCCG14307440.42236490955593553No Hit
AGTCTACC14112160.4166001172843563No Hit
TTAGCAGT13974550.41253778082136977No Hit
ACGGACGG13842350.4086351510676686No Hit
GTTCTAAT13840120.40856932002114243No Hit
TAAGACTG13676160.4037291144657957No Hit
GCGACAAG13656150.40313840628598047No Hit
TGCAATCG13529330.3993945976221046No Hit
CTAGCGGC13423860.39628105480725695No Hit
CCTGGCCA12930920.38172914625363014ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GTGGTCGT12545550.37035277387705046No Hit
CTGTCATT12351240.3646166166346777No Hit
CGACTCTG12333310.36408731140408873No Hit
CACATTAT12284070.362633714663754No Hit
ACATACTC12257370.3618455133443605No Hit
AAGCCTGT12193160.35994999249348947No Hit
TGTTGCGT11865520.35027784716442245No Hit
ATCTCAGT11810940.348666610160209No Hit
GTAGAATG11675490.3446680382983419No Hit
GTGAGTCA11623030.3431193850693014No Hit
TCAATAAC11346110.33494452704059535No Hit
TACATGTC11100130.3276830378816285No Hit
TAGCATGG11034240.32573792234099785No Hit
GCCGGTTG10921900.3224215726698118No Hit
GATCGGCG10276460.3033677651945554No Hit
CCGCTGTA10112160.298517522618662No Hit
CAACAGCA10107700.2983858605256097No Hit
TATAGGTG9982890.2947013883655534RNA PCR Primer, Index 39 (100% over 8bp)
TGGAAGAT9874520.29150223566957306No Hit
CGGAGCTG9802090.28936405508666396No Hit
GCGCAATC9789880.289003607966447No Hit
GAACGCGC9728520.2871922209642752No Hit
AACGTCGA9599730.28339025662252654No Hit
GACTAAGA9535110.28148263230570225No Hit
GATCGATT9328110.27537185803175257RNA PCR Primer, Index 42 (100% over 8bp)
CAGCTAAC9263210.2734559679333017No Hit
ATATTCGG9192900.2713803711255654No Hit
GATCTTCC9153280.2702107630254017No Hit
CTACAAGC8851080.2612896229984085No Hit
CATTCCAG8840750.2609846746976843No Hit
GTATCTAG8831890.26072312175049983No Hit
CTGTAAGG8696250.2567189409653861No Hit
CCGGCTGA8464460.2498763498109958No Hit
ATGTCAAC8304270.24514743710112144No Hit
CGTATGAC8107170.23932891724896935No Hit
CGAATCGT8097420.23904109092447173No Hit
CTCCAATG7940840.2344187477562831No Hit
TCATTGCG7913570.23361371966714972No Hit
CAGGAATC7582980.22385448842451675No Hit
AGTCATGA7516520.22189254611414624No Hit
AAGTGGAG7394850.21830076879090246No Hit
CATTGTGA7286430.21510014006248884No Hit
TCGTTCTG7275140.21476685193904493No Hit
TCGTCGAC7266370.21450795585024038No Hit
TCGTGTCA7011580.2069863897765223No Hit
CTTCTAGA6930980.20460702549400855No Hit
AGATGACT6875940.20298220899140862No Hit
AATGGTCT6841180.20195607124376372No Hit
TGAGTAAG6586490.19443745723491232No Hit
CGTACACT6109150.18034607080048168No Hit
TGGCGCGA5998960.17709319052392847No Hit
GCGGAGTA5862870.17307572544691488No Hit
GAACAGAC5509600.16264696589252742No Hit
GACAGTCG5501550.16240932467076272No Hit
TGCTCGAG5488000.16200931988133266No Hit
AGCCACGC5416400.15989564143681673No Hit
CTTGCTTG5094980.15040711454245487No Hit
CAATACAT4982610.14708987925180886No Hit
GTGGCGCT4971510.14676220005161153No Hit
AATTATTG4952970.14621488722533604No Hit
AACACGTA4611720.136140965867919No Hit
CACACATG4474720.13209663700061897ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
GTCGAAGC4288430.12659723536055092No Hit
CGATGGCA4251930.12551973168422645No Hit
AGGATCTT4241050.12519854702673575No Hit
CGTGTTAG4060860.1198792213435329No Hit
ACGCATTA3927540.11594352846332531No Hit
GTCCGACT3709300.10950094209836501No Hit
GCGCTCTC3655390.10790948392875813No Hit
CGACCTCG3552250.10486472422530319No Hit
CAGTACCG3543310.10460080962622538No Hit
GCCATCGC3484630.10286853796247963No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)