FastQCFastQC Report
Wed 20 Sep 2023
EGAF00001836116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338718575
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCTCC54747591.6163149599929678No Hit
GTGTGTGT39704041.1721837221356992No Hit
CGGTTGTT38324301.1314496112296175No Hit
CTCATAGG32583310.9619581683703057No Hit
GGTTAATG32523340.9601876720224157No Hit
AATCCAAG32239110.9517963400737618No Hit
GTCACAAT31647500.934330217939775No Hit
CTTCTGCT30635960.9044664881458007Illumina Single End Adapter 1 (100% over 8bp)
GCGTCGCT30464000.8993897072222862No Hit
AGTCTGAT28116650.8300888134050517No Hit
CCATAAGA28042720.8279061755027755No Hit
GTGATGTC27813700.8211448102602581No Hit
TCTCTCAG25648260.757214451554657ABI Solid3 Adapter A (100% over 8bp)
CGTAGTTC25595500.7556568162817761No Hit
CGGTATAT25451860.7514161276806269No Hit
CGGTCGGA25062970.7399349149954354No Hit
GTGTAGAT24521480.7239484873246175Illumina Single End PCR Primer 1 (100% over 8bp)
TTAGGTCA24450540.7218541232939469No Hit
CACGCCGT23256490.6866021445679499No Hit
GGCTACGG23025810.6797917710890228No Hit
TGCGGATT22904970.6762242076626592No Hit
CTCGGCCT22823500.6738189660841599No Hit
CAACTCGA22656430.6688865527968166No Hit
TTATACGT22655490.6688588011448737No Hit
ACTATTAC22526690.6650562343680149No Hit
TCTGTGTT21620080.6382903565297533No Hit
CGACGCCT21595070.6375519854498679No Hit
CTCAATTC21433240.6327742728605893No Hit
GCGCTCAT21263430.6277609664601358No Hit
CCTTGGTT20952760.6185890454930025No Hit
TTGACTCT20821060.6147008619175964No Hit
TCCAGATC20298350.5992688768249571No Hit
CTGCCTTA19954350.5891129531352097No Hit
AGACTCAA19948020.5889260723301047No Hit
ACAAGTGT19790740.5842826895454434No Hit
GTGCCACG19693790.5814204314009056No Hit
TAGGTGAA19627550.5794648256299496No Hit
TGGTTCCA19469860.5748093384013557No Hit
ATGTAATA19319150.5703599219499551No Hit
CTATCGCT19245290.5681793506600575No Hit
CCGTTACT19038470.5620733967719367No Hit
ACTGGTTA18905380.5581441761792957No Hit
CACCGGAG18880350.5574052146387307No Hit
ACCTATAA18835600.556084058868044No Hit
CACGATAC18644450.5504407309224184No Hit
TGGCCGCA18613650.5495314214757783No Hit
CAGCACAA18519540.5467530087477488No Hit
GACATCTA18391580.5429752413194345No Hit
ACCAGGCC18129890.5352493585567311No Hit
CTGTGTTG17674890.5218163780950011No Hit
CCTGGTGC17456550.5153703188554096No Hit
TTCGACAA17398090.5136444022888322No Hit
TCATCATG17236200.5088649183175148No Hit
CATGTATT17120390.5054458557520797No Hit
CCTTGTAG16974740.501145825852627No Hit
ACGCCTCG16865630.49792456761487025No Hit
AGCAACTA16649270.49153696398256286No Hit
ACAGTGCC16597880.49001977526623686No Hit
CATCAGTG16398710.484139672588077No Hit
GCATCGAA16262300.4801124355226164No Hit
TTAAGTGG16204740.47841308968662255No Hit
CCAGGATA15848560.46789757544297655No Hit
CATTCTTC15811900.46681526101720283No Hit
CACACCAC15755040.46513658130499635No Hit
TATGCCGC15451190.4561660074296191No Hit
CCTGTGCG15301990.451761170759531No Hit
ATTACGAC15230040.44963698846453876No Hit
TGCTATAC15030610.44374920979754356No Hit
TTGCTCTA15029680.4437217533759405No Hit
CTCTAGTT14920270.4404916382279891No Hit
TTCCTTAT14919190.44045975335128873No Hit
AAGGTGTT14914080.4403088906476416No Hit
ACTGTCAT14872440.43907955151263844No Hit
ACAACCTT14582600.4305225953433466No Hit
AACCGTTC14557220.4297733007408879No Hit
ACGATTCT14290900.4219107263308486No Hit
CCTCCATC14272940.421380492640535No Hit
TGGTTAGT14208160.4194679904991924No Hit
GACGCAAG14170700.41836205764623335No Hit
CCAAGACG14118780.416829221721897No Hit
GAGAAGCT13796190.4073053861897004No Hit
TATGAGGT13715690.40492878195416354No Hit
GCTTATAC13617030.4020160394215168No Hit
AACGCGCC13413170.3959974737139822No Hit
CCACCAGG13297130.39257162085073133No Hit
AGTGGCAA13242650.39096320595940154No Hit
AATGTAGG12922660.38151613031555764No Hit
AGTGCTCA12728270.37577714773983095No Hit
GAGCGTTA12571730.3711556120003162No Hit
CCATGCGG12279010.3625136294931567No Hit
CTGATTAC12166720.35919848800733767No Hit
ATCTAACC12163900.3591152330515089No Hit
CTGCGCTT12044920.3556025824683515No Hit
TGAGATAA11971080.3534226016391336No Hit
TTGATGAG11905960.3515000616662372No Hit
ATGAGACG11894750.35116910845530097No Hit
CGGCTTGA11875990.350615256337802No Hit
AATCAGCT11760680.3472109552893578No Hit
CATATCTC11749990.3468953540560921No Hit
TGTAGCAC11370780.33569992433984464No Hit
CCTATACC11288640.33327490232857765No Hit
ACGATAGA11167240.32969080600318423No Hit
GCTGACAA11117850.3282326633548219No Hit
ATTCCACA11085940.3272905833404619No Hit
ACGACGAT11056980.3264355962763483No Hit
AGGACCAG11026320.3255304200544656No Hit
TACCTGCA10997700.32468547082190574No Hit
ATTAGTGC10888880.32147277426400367No Hit
GCACACCA10883000.32129917882419057No Hit
GATGGTAC10833920.31985018831636264No Hit
GCCACTGT10797650.31877938787384186No Hit
CCTAACCT10646120.3143057625345761No Hit
CGATCTAA10512440.31035912335188587No Hit
ACTTAAGG10434020.3080439270270312No Hit
TCAATCGG10318820.3046428735123251No Hit
AGCGTTAT10294420.30392251148316857No Hit
TCAACTTA10247870.3025482142513147No Hit
GTCCGCGG10241020.3023459814685392No Hit
GACAAGGC9983600.29474616206093807No Hit
AGAGATCG9877690.2916193775319231No Hit
GCTAGTCT9852800.2908845492161155No Hit
CGACTTAC9820590.28993361229156095No Hit
TGCCGTAG9803300.28942315903401516No Hit
GTAGTGAC9576190.28271818278640315RNA PCR Primer, Index 43 (100% over 8bp)
GCGATCCA9357960.27627537108054967No Hit
GTAAGAGA8939240.2639134862916804No Hit
GCACGTCC8833930.26080441558305445No Hit
CAAGGTCC8813730.2602080502966216No Hit
GATTGAGG8784110.25933357803007995No Hit
TGTGTCGA8746020.2582090456657123No Hit
GAGAATTC8499260.25092394180035743Illumina Small RNA Adapter 2 (100% over 8bp)
ACACGAAT8489760.2506434729775301No Hit
TCTCTGCC8487130.25056582739815786No Hit
AAGATGGC8463350.2498637696500701No Hit
TCGGAGCT8416470.2484797298170022No Hit
CGCCAGCC8414860.24843219773229147No Hit
TGCGCCTG8351620.2465651610632809No Hit
TTGCAGAA8332990.24601514694019955No Hit
CTAGGCAC8229980.24297397920973185No Hit
GGTCTCGC8191360.2418337996373538No Hit
CGTATCCA8172850.24128732827835025No Hit
TAAGTCCT8042560.2374407721808584No Hit
CTATTGGA8006670.23638119049125073No Hit
ATGGAGAG7853180.23184970000538055No Hit
GAGTTCCT7816050.23075350975363543RNA PCR Primer, Index 1 (100% over 8bp)
TACCTCGC7733730.22832317359625168No Hit
CGCGGTCG7659780.22613994523329584No Hit
TCTGATAT7645710.22572455614517156No Hit
TTCTATGA7236380.21363989264539152No Hit
TGGCACAG7205300.21272231674923642No Hit
AAGAACGT6913910.20411959987727274No Hit
GCAAGCAC6913440.20410572405130128No Hit
TCTTAGTA6896560.2036073752376881No Hit
ATACGTTA6779540.20015258980113507No Hit
TTCAGTAA6776990.20007730606448143No Hit
GTCGACCG6696920.19771339673355673No Hit
AGAATGTA6615450.19530815515505756No Hit
ATAGCCTA6595200.19471031371692563No Hit
CTTCGCAA6454580.19055878467840154No Hit
TCCACAGG6367920.18800031855353666No Hit
GCTACCTC6331540.1869262705772779No Hit
TACAAGAA6313850.18640400810613944No Hit
TCTCATTC6302110.18605740768719284No Hit
AGGTCTTG6299860.18599098086073373No Hit
CACCACGG6247400.18444220249804724No Hit
AGCAGGAA6171510.18220169944916662Illumina Paired End Adapter 2 (100% over 8bp)
AACGGCCG6021410.17777029204849484No Hit
CACTTGCT5925300.17493283325250172No Hit
AAGCATCC5769440.17033137317609465No Hit
ATTAGGCT5755850.16993015514428167No Hit
CTTCGACG5728690.16912830954133531No Hit
GTTCACTG5675820.16756742673471628No Hit
TTCCGAGC5599340.16530950509578637No Hit
GGACGTTG5409730.15971164262249274No Hit
AACAGAAT5371760.15859065302220288No Hit
AATATTCG5328470.15731260088112972No Hit
TATGCTTA5215910.15398948817613559No Hit
CGAGTTCT5079800.14997110802086952No Hit
AGTAACCG4931770.14560081330054014No Hit
TATCACAT4865270.14363753154074882No Hit
GTATATCA4795700.14158361406663333No Hit
TGAATTGA4755320.1403914739544473No Hit
ATACATGT4685520.13833076618251597No Hit
GCCAACTG4584370.13534451129525446No Hit
AACAATAG4408440.13015052392683218No Hit
TGACGACC4236130.12506340994142406No Hit
TATTGACC3742620.11049349744105413No Hit
TATACGGA3530020.10421690041651835No Hit
CATGACTA3494910.10318034669341651No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)