FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365708669
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCATG40632891.1110726500169457No Hit
GTCATACA31562010.8630369656345225No Hit
GGATATGC31155690.8519264824974658No Hit
GTCTCCAC31154480.8518933960518175No Hit
GACCACTT30656850.8382861167559579ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
ACTCCGTT30280350.8279910367670282No Hit
ATCTGTCT29947050.8188772249202548No Hit
TTCGTTCG29594270.8092307486427126No Hit
ATACCGAA29499010.8066259430125786No Hit
TTAGGATC29034860.7939341465268902No Hit
TGAGCTCC28040010.7667307990448539No Hit
CGCCTCAT27422090.7498342895448289No Hit
CTTCCTGT26786020.7324414833600786No Hit
CCGCTGTA26552010.726042674148367No Hit
AGGATCTT25830440.706311941432266No Hit
AGAAGTAG25755960.7042753476538451No Hit
CAGTACCG25520780.6978445457632835No Hit
TCGTTCTG25452120.6959670950540142No Hit
TATACTAT25128700.6871234436064189No Hit
GCCGGTTG24830750.6789762481676365No Hit
TCGGATTG24544940.6711610109521358No Hit
ACGAGTTC24328930.6652543967996558No Hit
GCTGTAGC23777250.6501691651176035No Hit
CTATGGAG23616070.6457618318038832No Hit
CGTTCATA23561930.6442814184423942No Hit
CACTGCTT23326120.6378333897247593Clontech Universal Primer Mix Long (100% over 8bp)
CATTAAGT23201040.6344131809464981No Hit
CAGGCGCA22817090.6239143868913866No Hit
CTCAGAAC22815260.6238643470603645No Hit
GAGTCCTA22731470.621573179059641No Hit
CTCCAATG22661140.619650063586543No Hit
GACGCCTC21771180.5953148460913297No Hit
GCACTCTC21652250.592062803958306No Hit
CAATACAT21205220.5798391396622867No Hit
GCCTTAGG21085090.5765542845253143No Hit
TGCTGCCG21048800.5755619645975634No Hit
CGGAGCTG20754030.5675017236192451No Hit
GATCGATT20580080.5627452052551699RNA PCR Primer, Index 42 (100% over 8bp)
AAGGCCTG20366320.556900115485094No Hit
CAGAGGAT20026150.5475984491907137No Hit
ACGTGGTA19997770.546822421647325No Hit
GAGCTTAG19992500.5466783178716499No Hit
CTTAACAC19872420.5433948299431753No Hit
CTGGATGC19803490.5415099963080175No Hit
TTCGCATA19730450.5395127781343352No Hit
TTGAGAGT19720660.539245078710453No Hit
CCGGTAGG19643580.537137390090143No Hit
TCATTGCG19597610.5358803785972052No Hit
GGCCGCTC19381150.5299614595682445No Hit
TTGTCACA19263740.526750980573556No Hit
GGACCGGC19226360.5257288555005515No Hit
CAATCAGC19203730.5251100569344174No Hit
TTACAATT19059170.5211571837253878No Hit
GACTCGGT19053810.5210106189744165No Hit
GATTGGAT19024980.520222286554547No Hit
CGATGCTC18779580.5135120272470216No Hit
CCGACCGC18699640.5113261343006337No Hit
CCACATAT18639270.5096753667603106No Hit
GTGGTACC18509690.5061321092172414No Hit
GATGGCGT18417620.5036145314892713No Hit
GATATTGT18324090.5010570312731635No Hit
GAAGCGAT18210430.4979490929158149No Hit
TGCCTAGA18155630.4964506324021539No Hit
TGTGAACA17941760.49060253477338267No Hit
GAACAAGT17877090.48883418730224304No Hit
TGTCCTGC17693970.4838269228996593No Hit
CTTGCCGA17686340.4836182868828849No Hit
CAAGACGC17633840.4821827179601258No Hit
CTAATGAT17545720.479773149703487No Hit
GGTGAGAT17526910.47925880586659No Hit
CTGTAAGG17451310.4771915866178168No Hit
ACCTCGCA17252810.4717637688812895No Hit
TGACGGAA17160960.46925220687071No Hit
ATATGGTT17078400.46699467219903396No Hit
TAATTAGA17039590.4659334449629905No Hit
TATCAAGA17018560.4653583970687881No Hit
GTCCATCC16922370.4627281613605938No Hit
TAGATCCG16909290.46237049961755217No Hit
CCGAAGGT16863950.4611307149516874No Hit
TACACACA16840580.46049168169978494No Hit
TGGCCTAT16812930.45973561540046515No Hit
CGAACTGC16660970.4555803953337513No Hit
CTCACGCC16398900.44841430871303734No Hit
GACTTATC15974490.43680916954145266No Hit
GGTCGTAT15896000.43466292564150294No Hit
TAGCATGG15803650.4321376915459447No Hit
CTGTAGCA15706980.42949433063617093No Hit
CGCACCGA15656580.4281161844703222No Hit
CTACAAGC15554470.4253240712759806No Hit
GGCCTAAG15426110.42181417362025947No Hit
CCATTGAC15330560.41920143818083794No Hit
TTGGAACG15035650.4111373690187257No Hit
AGCGAAGA15033390.4110755711946221No Hit
AAGCAGGA14947300.40872151160299675No Hit
AGCCACGC14734790.40291060204536744No Hit
TTACAGCG14717740.40244438394759524No Hit
CGCGATTA14618520.3997312954044302No Hit
GATACGAG14585100.3988174532444567No Hit
GTCTTGTA14572640.3984767448867886No Hit
GTAGAATG14538120.397532824139862No Hit
ACAGGCTG14506970.3966810532456916No Hit
CGGATGCG14380310.3932176406788979No Hit
CCGCGTAC14364500.3927853293518727No Hit
CATCCTCA14190850.38803701423878467No Hit
CTCTCGAA14147770.3868590273970235No Hit
CGGCACTC14051850.3842361746147177No Hit
TGGAAGAT14029170.3836160088400858No Hit
GCGGAAGT13986880.38245962389259086No Hit
AGATGGAC13960000.3817246126041382RNA PCR Primer, Index 16 (100% over 8bp)
CCAGCCAA13462250.36811405200788394No Hit
AGATGACT13444930.36764045098422315No Hit
TCATTCAA13401690.36645808907526883No Hit
TCAAGGTT13271670.36290280009741854No Hit
CTCGAACA13060720.3571345474449226No Hit
TTAACCAG12668710.346415359379955No Hit
ACCATAAG12597480.3444676341538953No Hit
GTAGCTTC12388550.3387546167247132No Hit
AACCAACG12018420.32863371909841166No Hit
CATTCCAG12003560.3282273847328459No Hit
CAATATGG11978180.3275333896993292No Hit
TCACCGAG11946230.32665974346919296No Hit
AAGCCTGT11539770.3155454321483421No Hit
CTCACCTT11505870.31461846478678906No Hit
AGAGTATT11426950.3124604628937577No Hit
CACACATG11367960.31084743030797557ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
ATGTCAAC11105270.3036643903018881No Hit
ATTGAGTA11014770.30118974292075096No Hit
TAAGTCCT10808950.29556176586013605No Hit
GACAGTCG10742080.2937332612150903No Hit
CTCTTAAT10739930.2936744712496821No Hit
CTGGCCTC10596550.289753864161202No Hit
GAGTGGCA10551960.28853458762280526No Hit
GAGAGCGG10248170.28022770223147214No Hit
GATTATCT10091380.2759404098238645No Hit
GTTGTGTG9915180.2711223670773853No Hit
ACCACCAA9466450.25885221769243866No Hit
TCTCTGCC9465330.2588215922220865No Hit
TCTGCGCA9460390.2586865120224974No Hit
AATGCGAA9373880.2563209678794899No Hit
GACAAGGC9230610.25240336864970514No Hit
CGCGTGAA8917930.24385339358745142No Hit
TGGCTTCC8825140.24131612805711203No Hit
GGTTGCAG8544250.23363542415780142No Hit
ACTTCTAT8394990.22955403334997235No Hit
GACTAAGA8340840.22807334654678366No Hit
CTGATCCT8320440.22751552547965442No Hit
TAAGACTG8254790.22572038072195658No Hit
CGCTACAA8144900.22271552988534707No Hit
GGATACTA8021300.21933579047862276No Hit
TTGATGAG8001810.21880285260615467No Hit
CCAACAAT7978920.2181769445558317RNA PCR Primer, Index 36 (100% over 8bp)
AACTACCT7978610.2181684678631449No Hit
ATAACGCG7810370.21356808470952598No Hit
AGGTCTTG7765000.21232747971856253No Hit
TAACATTA7711260.21085800402505633No Hit
TCGGCCAG7649830.20917825166457837No Hit
AAGTTACG7579160.20724583917369485No Hit
TATCACAT7467970.20420544091614082No Hit
CACGATAC7465110.2041272365900629No Hit
CGGACATC7329920.20043057825353325No Hit
AAGCGCAC7328650.2003958511576875No Hit
CAAGGTCC6772490.18518811759422635No Hit
TTCGACAA6324110.17292753866876479No Hit
GCGCTCAT6132500.16768812226324337No Hit
CGGTTGTT6101010.16682705435128747No Hit
TATTGACC6074900.16611309807370195No Hit
CCAGGATA5601000.15315469593092965No Hit
GTCCGCGG5372410.1469040921203867No Hit
GTCACAAT5231150.14304145467221616No Hit
TACAAGAA4962420.1356932558795865No Hit
TGCCGTAG4910540.1342746403422009No Hit
GGCTACGG4730750.12935843202557498No Hit
TGAGATAA4447310.1216080004928732No Hit
CGACGCCT4355690.11910272764138385No Hit
AGTAACCG4335420.11854846131634905No Hit
TCTGATAT4078450.11152182996241743No Hit
AACGGCCG3972930.1086364731485214No Hit
CATCAGTG3960610.10829959297464725No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)