FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365211625
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGTCT42173431.1547669108287557No Hit
CCGGTCTT38740441.0607668909772519No Hit
TTGCGTCG35806350.9804274439511611No Hit
GAACAGAC33579370.9194496478582794No Hit
GGCTGAGT33014530.9039835465259355No Hit
GCCTACAT32589270.892339338869621No Hit
TGTCGGCT31830940.8715752134122237No Hit
GCAGATCT31827260.8714744499165381No Hit
GTAATCTT31327020.8577771860356307No Hit
TACATGTC30006670.8216241747507352No Hit
TCTACCGT29733230.8141370089191438No Hit
TCCGGCGA29644440.8117058157718829No Hit
GTCCGACT29420400.8055712903443311No Hit
CGCCGGTA28679330.7852797675868066No Hit
CTCTGAGA28102550.7694867325211786No Hit
TAGGTCTC28038970.7677458240821332No Hit
TTCCGGTT27593550.7555496077103241No Hit
TAGCTGGT27214910.7451819202085914No Hit
GGCACGTT27045370.7405396802470349No Hit
TATATAGC26884020.7361216938261481No Hit
TGATTCGC26247590.7186953591633344No Hit
ATAGTTGC26210420.7176775930941409No Hit
CGAGGAGT25856760.7079938925821433No Hit
ATAGGTAT25690370.7034379039823828No Hit
GTATCTAG25611830.7012873700282678No Hit
CGAGCCTT25470940.697429606738285No Hit
ATGAATTG25452960.69693728944143No Hit
GTTCGGAC25222570.6906288922210513No Hit
ATGTGCAT24390970.6678585327068929No Hit
AACGCTGG24001330.6571896499734914No Hit
ACACTAGC23980140.6566094384317586No Hit
ACCTGAAC23972780.6564079114403875No Hit
AGTCATGA23879070.6538420018804166No Hit
AGAAGAGC23747460.6502383378404234No Hit
TCCTCCTA23439160.6417966569382889No Hit
TAGTCGTG23294570.6378375825249265No Hit
ACTTGTCC23257660.6368269356157543No Hit
TAGCGCTG23156850.6340666182244336No Hit
AATGGTCT23046720.6310511063277353No Hit
TTAGAGAT22598730.6187845197972546No Hit
GTCAGATG22581190.6183042503096664No Hit
TCGAGCAA22565160.6178653267129709No Hit
AAGACACT22539690.6171679228447342No Hit
TGCAGTGT22449580.614700586269673No Hit
TTGACCGA22274990.6099200703153959No Hit
CAGCGTCT22271930.6098362832782226No Hit
GGTAATTA22009100.602639633938268No Hit
GGTCCGTG21888300.599331962666851No Hit
GTTATCCG21616780.5918973690938781No Hit
TCGGCAGC21523340.5893388525077755No Hit
CTTATGTA21311610.5835413919258456No Hit
AGACCATC21290650.5829674781025932No Hit
GGCGTGTC21214180.5808736236148014No Hit
TGATAAGG20935670.5732476341627953No Hit
GCAGTGGT20929620.5730819767853775Clontech Universal Primer Mix Long (100% over 8bp)
TCGTGAAG20511720.5616392961204343No Hit
GGCTCTTA20182330.5526201418150367No Hit
AGCCATTG20065970.5494340438916752No Hit
CTCTATAG19950020.5462591723360394No Hit
TCCTTACA19762650.5411287222853325No Hit
ATCGGCGC19422850.5318245277652375No Hit
ATGCTAGG19366790.5302895273391147No Hit
TCACGCCG19218630.5262327013823834No Hit
TTAATACT19184210.5252902341211072No Hit
TACTGTTG19181130.5252058994562399No Hit
AACGGATA19029240.5210469409345883No Hit
AGTTACAT18955900.5190387901808985No Hit
ATCGCTAA18910570.5177975920125762No Hit
TTGTACTC18815180.5151856817263142No Hit
GTCCAGAG18622730.5099161342413456No Hit
GTACTATA18557350.5081259393098453No Hit
GGAATATC18421030.5043933089479284RNA PCR Primer, Index 37 (100% over 8bp)
AATCTGTA18233560.49926012075875187No Hit
TCTATGAA17961570.49181265793497125No Hit
GCATCCGT17960720.4917893837579787No Hit
AACCTTCT17960080.4917718596717725No Hit
ACCGAACT17880270.4895865513590922No Hit
GCGCAATC17782210.4869015327756886No Hit
ATGGCGCT17730430.4854837246760697No Hit
CGTAATGT17725650.48535284165721726No Hit
CTATCCTG17617680.4823964735514648No Hit
GCGTACGA17582270.4814268987193384No Hit
ATAGGAGG17135960.46920631291514886No Hit
TAACCTCT17109290.4684760513852756No Hit
AGGCGGTT17067740.4673383548511086No Hit
GCGCGAGG17059470.467111910799663No Hit
CTGACTGG16822680.4606282727172225No Hit
GGCAACAC16490450.45153135527928495No Hit
TCTGACCG16479740.4512381006491784No Hit
TCAATAAC16293120.4461281866369944No Hit
AGTCTACC16050920.4394964152633422No Hit
ATCATCTC16044250.4393137814274121Illumina PCR Primer Index 7 (100% over 8bp)
ACGGACGG15829660.4334380100852485No Hit
AATTAACA15737840.4309238513423553No Hit
TAGGATCA15718730.43040059308079254No Hit
CTAACATA15661690.42883875889766654No Hit
GAGTATAA15598370.42710496961864236No Hit
CGGAGAGA15364290.42069553508873103No Hit
GATACACC15217170.41666718577208484No Hit
GACGATGT15056730.41227411641127254No Hit
TACGAGCG14819130.40576829940722725No Hit
CAGATAGT14811650.40556348664969255No Hit
ATTAATCA14702140.4025649512115065No Hit
GCGCCTAA14660130.4014146592403788No Hit
CGATGGCA14558910.39864311548133224No Hit
TACGTACC14084420.3856509222563767No Hit
GAAGGTGA13536390.37064510200079204No Hit
GTAATTCC13510970.3699490672017902No Hit
GTGCGGTG13418790.36742505116040597No Hit
AGGCCAGA13390440.36664878890424146No Hit
TGGCGCGA13087880.3583642771502687No Hit
TCGGTAAT12917280.3536930129209332No Hit
AACTCTAC12378420.33893827996302145No Hit
GCCTCATT12292570.33658758808677025No Hit
CGTGCCAC12244480.33527081729668384No Hit
GGATCTCT11715130.32077648130724207No Hit
ATATTACA11631150.31847699262037454No Hit
TTCGGAAG11539330.3159628338774813No Hit
GCACACCA11517650.3153692054572469No Hit
AGCGCTTC11507640.3150951177964283No Hit
AGGAATAC11359340.31103445844583943No Hit
AACTTGAA11222560.3072892326469619No Hit
ATTATCGT10562490.28921560204990737No Hit
CTCTCTGC10484170.2870710920004258No Hit
CATATCTC10111100.2768559188114562No Hit
AGACAGAG10077130.2759257731732937No Hit
GATATATG10058830.2754246938333357No Hit
CCGAATAG10011120.2741183279694342No Hit
TATTAGAG9949400.27242834890592543No Hit
AACGTCGA9857350.2699078924445519No Hit
ACTAAGTG9820930.2689106624138813No Hit
AGGTGTAA9679370.26503455359615125No Hit
GCTCCAGA9572400.26210556687509606No Hit
GGATCAGA9367610.2564981331029646No Hit
CATCGAGC9276790.2540113557447685No Hit
AGCATAGT8571310.23469433646861595No Hit
GATTGAGG7813690.21394965179435346No Hit
AACTAGTC7337010.20089749333691118No Hit
CACGCCGT7268230.1990142016974405No Hit
TGCATCAA7018600.1921789866354884No Hit
CCTTGTAG6777480.18557678715730913No Hit
CGGTCGGA6506590.1781594438566954No Hit
ACAAGTGT6470390.1771682377306582No Hit
TTAGGTCA6065540.1660828841360129No Hit
GTGTAGAT5994300.16413223429018722Illumina Single End PCR Primer 1 (100% over 8bp)
TATGCTTA5960370.1632031839074126No Hit
GACATCTA5815750.15924328805250929No Hit
CCTTGGTT5700130.15607745235382361No Hit
CCTGGTGC5675900.15541400140261144No Hit
CTGCCTTA5654360.1548242063762346No Hit
TGACGACC5427110.14860178670380494No Hit
TCTCATTC5409610.14812261247160466No Hit
TATGCCGC5142200.14080055638973704No Hit
TGGCACAG4826980.13216939630549823No Hit
ACCTATAA4785330.13102896163286148No Hit
TGTAGCAC4761560.13037810611861No Hit
ACGATAGA4741770.12983622851545318No Hit
GTCGACCG4465830.12228060922211882No Hit
TCGTGGAG3777350.10342907348581798No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)