FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350448187
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGCC53576401.5287966092402698No Hit
TACTGCAC35836441.0225888256628362No Hit
GCGACAAG34850300.9944494305516267No Hit
GGAACCTG33478530.9553061263233187No Hit
GCCATCGC33327100.9509850881323008No Hit
CATACGTT31337800.8942206341047501No Hit
CGCGCGCT30781810.8783555213541452No Hit
GTGGACAC30200010.8617539231270156No Hit
GTGGTCGT30120180.8594759829646372No Hit
GCCTGGTC29809000.8505964963088823No Hit
TCCGTATG29793010.8501402234390786No Hit
GGTTCAAT28636220.8171313495766493No Hit
CAGAGTGC28121190.8024350258658922No Hit
CGTACACT27694260.7902526258467988No Hit
CGTTCGCG27009040.7706999494336091No Hit
TCGATTGC26859050.7664200014822734No Hit
TGGCTGTG26280720.7499174193188222No Hit
ACTGAGGC25819280.7367502802917911No Hit
CCTGGCCA25786340.7358103410590622ABI Solid3 GAPDH Forward Primer (100% over 8bp)
ACCTAGGT25605470.7306492357456539No Hit
TTAAGGAC25557110.7292692885296621No Hit
TTGTGGCT25062500.7151556472455085No Hit
GGACACAT25026590.7141309593934353No Hit
GGCGCCAT24886400.7101306533510473No Hit
TACTCCGG24413490.6966362191509925No Hit
GGCCTGCT24378310.695632361767647No Hit
CTTGCTTG24085140.6872667884568054No Hit
CTGCTCAG23516160.6710310075024014No Hit
CATTGTGA23436130.6687473603622894No Hit
TATGGTGG22957350.6550854263657526No Hit
TACTCAAT22658510.6465580602361627No Hit
TGTACGCC22489720.6417416563778656No Hit
TCCGCGAT22035800.6287890997136191No Hit
GTGGCGAA22011790.6281039770366967No Hit
GGAGAACC21467090.6125610231791555No Hit
ATGGTCAA21338840.6089014237074651No Hit
CGTGTTAG21239920.6060787525204118No Hit
GAGGTGCG21211350.6052635107511628No Hit
AATTCATT21204770.6050757511837263No Hit
CGTGTGGT21194560.6047844099704245No Hit
CTGTCATT21046950.600572375054119No Hit
TGTCTTAA20985020.5988052093989004No Hit
AGGTTATC20542970.5861913618631447No Hit
GCACCACT20032190.5716163114292271No Hit
CATCTCCG20011230.5710182201627426No Hit
CACTCTCG19992860.5704940342579087No Hit
TACAGCCT19985650.5702882977106113No Hit
ACAGCGGA19752980.5636490851641929No Hit
CCGGCTGA19731050.5630233150557004No Hit
GTTACCAA19595310.5591499892678857No Hit
TCCTGGAA19383770.5531137189190252No Hit
GGTGTACG19229900.548723055599657No Hit
TCCGACTT19191310.5476218942459531No Hit
GGTTGGCC19017570.5426642426887488No Hit
ATAAGCCA19003770.5422704612251283No Hit
TGTGAGTC18966880.5412178091821602No Hit
CGACCTCG18846280.5377765016087813No Hit
GAAGGCAG18650620.5321933652919711No Hit
GAGACTGA18564930.5297482106819973TruSeq Adapter, Index 15 (100% over 8bp)
ATCATGGA18441870.5262367072824948No Hit
TTATGCTA18423940.5257250767286749No Hit
ATTCTGGC18370410.5241976041382688No Hit
AATCGCGG18356730.5238072468612885No Hit
ACCAGCGG18304390.5223137307883975No Hit
AGCTCACG18267580.521263361536523No Hit
ATTGCCAG18223040.5199924175952436No Hit
CGCGTCGG18041010.5147982118109802No Hit
GTTCTAAT17964700.5126207144567136No Hit
AGCAAGCT17905360.5109274541631457No Hit
GTGTTAAG17858090.5095786099758022No Hit
ATAACTGA17711560.5053973927392582No Hit
GAGTAATT17700490.5050815115217018No Hit
CGCTTACC17691660.504829548454762No Hit
TGGAGTTA17419600.49706634664370514No Hit
GGAGTGCA17305000.49379624840233516No Hit
CACGGCAA17274930.49293820430008384No Hit
ATGCAACT16930520.4831105032938864No Hit
GCTCACGT16835490.48039883282375206No Hit
CCTACTCG16735980.47755932605238444No Hit
GTTGCAAC16457550.4696143570005115No Hit
CGCAAGTG16444410.46923940856341195No Hit
GTAGCACA16385120.46754757501427735No Hit
TAGGTGAA16305730.4652821902029129No Hit
GTTGGTCG16273590.46436507888111855No Hit
CCAGCGTG16240030.4634074480174155No Hit
TGATCCAT16069560.45854310554615596No Hit
TAAGCAAC16011960.4568994959588705No Hit
GTCCTCAA16010170.4568484185081545No Hit
TCGTGTCA15999970.4565573626437394No Hit
TGAGTAAG15954530.45526073730265865No Hit
CGTATGAC15740090.44914171577666057No Hit
AAGCTCCA15503880.4424014897243569No Hit
ACATCCAG15497730.4422260001590478No Hit
GTGAGTCA15198570.433689502865084No Hit
CCACTACA15115700.43132481664115446No Hit
AAGTGAGA14974240.42728827129015795No Hit
TGCTTCTT14965830.4270482928764588No Hit
GGCACTCC14877950.4245406468603018No Hit
TATAGGTG14831430.42321320383945943RNA PCR Primer, Index 39 (100% over 8bp)
GCATGTAT14821450.4229284256505513No Hit
GTTCAGGA14808670.4225637497733723No Hit
GGCGGAAC14539980.4148967105371272No Hit
CCGACACA14205060.4053398056243903No Hit
ATATTCGG14195900.4050784260441901No Hit
CAATTAAG14088290.4020077866746105No Hit
GCCGTCCT13560250.38694022406228057No Hit
ACGCATTA13197600.376592046686776No Hit
TTACCTAC12993330.370763224978533No Hit
ATGTAATA12979170.370359170954992No Hit
GCGTCGCT12860950.3669857764166433No Hit
GAGCCGTC12473330.355925082871095No Hit
CACTTGCT12469580.3558180770385895No Hit
GGATTGTG12364700.35282533791507387No Hit
TAATTGAT12273650.35022723630183883No Hit
CGAAGCAA12272210.35018614606215664No Hit
GATCTTCC11744610.3351311388008408No Hit
AGCTTCAC11635410.3320151289582788No Hit
TGCCAGGT10912680.31139210887114677No Hit
CTAGTCCG10899900.3110274329939678No Hit
ATCGCGTG10842030.30937611898674194No Hit
AGGTAGCC10715020.3057519027770002No Hit
AACGAGAT10476880.298956604389567No Hit
GGAAGGCG10475970.29893063764087896No Hit
TCGCGGAT10454740.2983248419544542No Hit
ACATACTC10418550.2972921643335538No Hit
CGTGCAGG10145430.2894987155405087No Hit
CCGTGGCG10021520.2859629574856382No Hit
CTTCGACG9860230.2813605652923523No Hit
ATCCTAAC9777530.2790007299994963No Hit
ACGTGCGC9739450.2779141214390132No Hit
ACTCGGAA9301890.2654283955533775No Hit
ACGCTATT9225010.26323463331257013No Hit
TGTACCTA9189360.2622173645315506No Hit
AGTTCGTC9050480.2582544391933179No Hit
ACAGCACG8945500.2552588465809355No Hit
GTCAACGT8798010.25105023585127006No Hit
TATCTACT8795800.25098717374731344No Hit
GACGCTCA8626010.2461422350003483No Hit
ACGCGACC8450320.241128940410241No Hit
TTCCGAGC8209130.23424661061236993No Hit
TCGGAGCT8105660.23129410568187644No Hit
CACACCAC7653830.21840118693494626No Hit
GTATATCA7587600.21651132125845468No Hit
TTCACTTG7455450.21274043572095863No Hit
TTCTATGA6956140.1984926804600647No Hit
AACAGAAT6559770.18718230663867008No Hit
GCGATCCA6482300.18497170881354852No Hit
CCTTAGCC6389340.1823191055629573No Hit
AACAATAG6153990.1756034195149082No Hit
CAGATTCA5908060.16858583434475008No Hit
AATCAGCT5151860.1470077515338951No Hit
AGAGATCG5066540.14457315483272853No Hit
TTGACTCT4919330.14037253387189016No Hit
AGAATGTA4700140.1341179716247184No Hit
CTGTGTTG4586970.13088867827414385No Hit
GGTCTCGC4586530.13087612292312986No Hit
CACCACGG4558160.13006658813161445No Hit
CGCATATA4414920.12597925067878865No Hit
TCATCATG4264180.12167790156095172No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)