FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001836126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412453531
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGGTAC51998411.260709536755063No Hit
GTCGAAGC48415281.1738359926902893No Hit
GCTGGAAT45652371.1068488100784377No Hit
GCGCTGGC38865920.9423102744633794No Hit
CAACAGCA38711950.938577247869409No Hit
GTTGCCTT38598080.9358164520114146No Hit
CACATTAT37996900.9212407494215391No Hit
TGTTGCGT37790810.916244065322355No Hit
CTCCTTCA37229900.9026447151448923No Hit
ACTCTTGT36949950.8958572838596938No Hit
CTGTTCGC36415890.8829089161076912No Hit
AACACGTA34582040.8384469376745376No Hit
TCTCGAGT34005740.8244744545537663No Hit
TTATGACG33816110.8198768457142872No Hit
CAATCGTA33606630.8147979705379221No Hit
CGACTCTG33594260.8144980579642559No Hit
GCCATGCG33255840.8062930124363513No Hit
GATGTCCA33100530.8025274973339966No Hit
GAACGCGC33009950.8003313711478446No Hit
CACAGGCA32670770.7921078993017518No Hit
CGCTGAAG32644620.7914738884851492No Hit
TTATCGTC31774480.7703772088691367No Hit
AACAGTGA31449370.7624948663610787No Hit
ACACTGTG31042660.7526341191633537No Hit
TAGAGTAG30909760.749411937995992No Hit
CTAGCGGC30848230.7479201335774235No Hit
GCGAGATA30658780.7433268888659363No Hit
TCTAGTGA30556790.740854125455408No Hit
TTCTTGCC30259550.7336474954314308No Hit
CCGTACAC28882070.7002502786186597No Hit
CAACGTAA28462670.690081860397505No Hit
AGCTGTGG28446750.6896958775217759No Hit
TAGCCACC28399980.6885619315985441No Hit
CGACAGTT28264170.6852691970286466No Hit
TCCGATGC28261500.6852044624634333No Hit
TAATACCA27884540.6760650086421494No Hit
TGTGGAGC27799650.6740068373908527No Hit
TGAGCGTA27663100.6706961614058773No Hit
TGCTCAGC27326080.6625250590956876No Hit
GGTCAACT27197480.6594071320970216No Hit
GGCCAATA27182700.6590487886985746No Hit
TAATAGGC27039140.6555681541734698No Hit
TTGTGTAC26872850.6515364272636085No Hit
ACTACATA26854340.6510876494351069No Hit
CGTTGTCT26854270.6510859522742212No Hit
GTCTGGCG26850590.650996730101942No Hit
TCTCTATA26732110.6481241640770437No Hit
TCGCACGC26675750.646757707112465No Hit
GATCGGCG26656840.6462992312217591No Hit
GCGGAGTA26555290.6438371356796555No Hit
TGAGGCGG26493670.642343149197091No Hit
GAGACCAT26459120.641505479074199No Hit
CTTAGATT26296260.6375569130477392No Hit
GCAGTTAA26196870.6351471870415385No Hit
AGTGGATG26112180.633093864821344No Hit
ATTGACCT26044320.6314485885683931No Hit
CGGCCAAG25799040.625501736824748No Hit
TCGTCTGG25548690.6194319621426638No Hit
CTCGTATC25534710.6190930148686253No Hit
CGAATCGT25506820.6184168174814341No Hit
AGAGCCGC25450610.617053997290182No Hit
AGGCGTGC25309030.6136213681729883No Hit
AGGCCGAC25210010.6112206128743265No Hit
ATCTCAGT25014800.606487716067098No Hit
AGCACAAC24970910.6054235961917368No Hit
ATATACAA24968700.6053700143980583No Hit
GCAACAGC24864910.6028536097076109No Hit
TTCACGGT24730140.5995860900994444No Hit
TGTAGACG24574760.595818877836204No Hit
GCCACGAC24550060.5952200224950916No Hit
CTGAACTA24486760.5936853041512693No Hit
TAATGATT24426610.5922269580473054No Hit
ATAATATG24364070.5907106660216712No Hit
CAAGAGAG24193280.5865698359120121No Hit
CGAGAGGA24140750.5852962378930391No Hit
GCCTGTGA24047970.5830467723647637No Hit
TACGTCAG24032260.5826658809716918No Hit
AATTGCTA23449280.5685314402121097No Hit
CCATCTCC23438060.5682594095672805No Hit
GCTTCTCA23398860.567308999471264No Hit
CCAATCTA23372520.5666703820751142No Hit
CCTTACTG23266930.5641103361047477No Hit
CTTGCGAT23189670.5622371553899971No Hit
TGCTAACT23165540.5616521197875258No Hit
ATACTTAG23141110.5610598106384013No Hit
TAACGGTC22895030.5550935627703476No Hit
TCGTCGAC22889110.5549500314497247No Hit
GGTAGCGA22620890.5484469958386657No Hit
CAGCTAAC22445830.5442026389149763No Hit
AGTTCCGG22172220.5375689219157151No Hit
CAGGCTAG22168900.5374884279994199No Hit
AGGATTGG21831460.5293071427239158No Hit
GAGGTATA21695800.5260180449273448No Hit
TTAGCAGT21431200.5196027767792344No Hit
CCAGATTC21332680.5172141440583279No Hit
GGTGCGGA21237740.5149123089941494No Hit
GAGTGAAC21189190.5137352066941088No Hit
AATTATTG21000680.5091647524288015No Hit
ACATTCCT20955390.5080666893357255TruSeq Adapter, Index 27 (100% over 8bp)
ATTCGATC20939910.507691374328421No Hit
CGTCCGAA20926860.5073749750490074No Hit
CATAATAA20626420.5000907605273963No Hit
CGAACGAG20474850.4964159223066513No Hit
CTTCTAGA20077160.4867738664116322No Hit
AAGTGGAG20006430.48505900656236595No Hit
TCCAACCA19987550.4846012580263254No Hit
CGTGATCC19969690.4841682395489057No Hit
TAACTATG19765210.47921059015009376No Hit
TGCTCGAG19563670.4743242215084831No Hit
GGCCGTCA19479970.4722949019922441No Hit
CGTCGTGG18946630.45936399075220913No Hit
AGAACACA18656310.45232513720436546No Hit
CTACGGCC18647440.4521100826749862No Hit
TAGGCATT18636290.45183974919104275No Hit
GATTACGC18289380.44342886229285305No Hit
CAAGCACT17973330.4357661808937211No Hit
GTCATTAG17741360.4301420321699222No Hit
GACCTGTG17547100.42543216826042884No Hit
GCAGCTGG17446860.4230018338720441No Hit
TCTCCACG17429650.4225845747457063No Hit
GTGTCGGC17406160.4220150560427618No Hit
CAGGAATC17336990.4203380186360921No Hit
ACTCATAG17072580.41392735706752865No Hit
TTGAAGGC16869580.40900559049887253No Hit
TCGCAACA16801070.4073445548948397No Hit
TGCAATCG16784230.4069362664760409No Hit
AGCGTGCG16584490.4020935391143493No Hit
TGCCTGAC16521530.4005670641234006No Hit
ATGCGGCA16479620.3995509496559505No Hit
TATCCGAC16276030.39461487844554305No Hit
GGAAGAAT16049050.3891117130477421No Hit
TGTATATT15995460.3878124151639279No Hit
CTTACAAG15239520.36948453230722855No Hit
AGTGTCTC14509950.35179599420134433No Hit
GCATAACG14405560.34926504241759054No Hit
CAGTGGTC13815100.3349492478947889No Hit
TATAATCC13778750.3340679364919778No Hit
AGAACGGT7688990.18642075826961463No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)