FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403037044
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGGTT51649251.2815013103361288No Hit
TTCGCATA48281951.197953158866459No Hit
TAACCTCT44652151.1078919584374483No Hit
ACGCCTCG44341861.100193162393281No Hit
GTTGCAAC42093461.044406727040207No Hit
CCGAAGGT41063931.0188624249635971No Hit
CCTGGTGC40661461.0088764942410604No Hit
GCATCCGT40601861.0073977219821015No Hit
GTGTAGAT39610790.9828076746215914Illumina Single End PCR Primer 1 (100% over 8bp)
TGTTGCGT39028560.968361607971698No Hit
CTGCTCAG38702060.9602606156470322No Hit
TAACGGTC38114190.9456746114880695No Hit
GCGCTCAT38046850.9440037973283667No Hit
TACATGTC37221750.9235317337232157No Hit
TACTGCAC36688760.9103073910000193No Hit
CGAGGAGT36686910.9102614895121155No Hit
GCGCTGGC36532800.906437771511643No Hit
GTAGTGAC36298070.9006137411031628RNA PCR Primer, Index 43 (100% over 8bp)
ATTCGATC36245740.8993153492858588No Hit
AATTGCTA36211800.8984732430699348No Hit
ACTACATA35981190.8927514365155973No Hit
CTATCGCT35534070.8816576671795955No Hit
GAAGCGAT35377040.8777614992630802No Hit
TTCCGGTT35248160.874563778311157No Hit
ATAATATG34221130.8490815052722549No Hit
TCTCGAGT33762070.8376914852521596No Hit
CGGACATC33726880.8368183645173817No Hit
ATATACAA33690310.8359110037537889No Hit
TGTAGCAC33596310.8335787119359679No Hit
TCATCATG33410710.8289736761765254No Hit
TATGCTTA33356850.827637322588144No Hit
AGACTCAA32999090.8187607191759773No Hit
GAGTCCTA32974070.8181399325665955No Hit
ACAAGTGT32770020.8130771225088679No Hit
GGTCGTAT32467750.8055773156176681No Hit
TACAAGAA32321380.8019456395179397No Hit
ATGGCGCT32217010.7993560512517058No Hit
GTAATCTT32154710.7978102876320222No Hit
TCGCACGC31853170.7903285932198332No Hit
AAGACACT31852180.7903040297209007No Hit
GAGTGAAC31755590.7879074758200142No Hit
AAGAACGT31507300.7817469999110057No Hit
AACGCTGG31182940.7736991044426179No Hit
CGTAATGT31124970.7722607751162447No Hit
TGCGCCTG30897860.7666258092146984No Hit
AACACGTA30667520.7609107017964334No Hit
TGCTCGAG30641010.7602529458805777No Hit
AGGCGGTT30420760.7547881876585022No Hit
CAATCGTA30262290.7508562910162669No Hit
GCGCCTAA30147000.7479957599133245No Hit
CAGAGTGC30128180.7475288053174587No Hit
TCTATGAA29858620.7408405863556303No Hit
TCGGCAGC29718340.7373600129917587No Hit
TAATAGGC28928350.717759085192179No Hit
TGCTATAC28555350.7085043527661442No Hit
CTCGTATC28508970.7073535900585853No Hit
CGGCACTC27533160.6831421679442449No Hit
GAAGGTGA27247830.6760626698125545No Hit
GACGCAAG27242740.6759363786917809No Hit
CAGATAGT27215300.6752555479738979No Hit
GAGTATAA27079150.6718774465803199No Hit
CTCCAATG27039530.6708944103907233No Hit
ACATTCCT27019320.6703929676498918TruSeq Adapter, Index 27 (100% over 8bp)
AACCAACG26952230.6687283563939597No Hit
AGAAGAGC26709470.6627050887163612No Hit
AAGGCCTG26547940.6586972685319715No Hit
GTTATCCG26125820.6482237895730497No Hit
TAGGTCTC26010870.6453716944192356No Hit
GCAACAGC25641200.6361995846714279No Hit
GTCGAAGC25609710.6354182669124577No Hit
GTGTCGGC25540430.6336993182194935No Hit
CTGTTCGC25379020.6296944754289137No Hit
CTCTGAGA25252870.6265644901861677No Hit
CAACAGCA24948980.619024488478533No Hit
CGATGGCA24931580.6185927663760853No Hit
CTTCTAGA24784140.6149345418482178No Hit
ATATGGTT24755250.614217734288464No Hit
TTGTGTAC24612630.6106791017452976No Hit
AGGTTATC24439610.6063861961036019No Hit
TCGTTCTG24362080.6044625515862011No Hit
TCCTTACA24289490.6026614764473114No Hit
ATGCGGCA23913840.5933409932413062No Hit
CAGGAATC23734380.5888883007984745No Hit
CGTCCGAA23690040.5877881537856853No Hit
GACCACTT23353420.5794360679163774ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TGTCCTGC23348400.5793115136086597No Hit
AATGCGAA23040690.5716767315314073No Hit
GTCCGACT22999970.5706664025652193No Hit
TGAGCGTA22827050.5663759780850318No Hit
CACACATG22384210.5553884024615862ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CTCTATAG22288510.5530139308981237No Hit
CGAGAGGA22283580.5528916096357633No Hit
GCCTGTGA21792360.5407036480745924No Hit
TGCAGTGT21599920.5359289008679807No Hit
AACCTTCT21376890.530395166355974No Hit
GGCACGTT21197980.5259561203014381No Hit
AATTATTG21033200.5218676623680278No Hit
CATCCTCA20646410.5122707777699957No Hit
ACATACTC20306780.5038440089392875No Hit
GTATATCA20111130.4989896164482588No Hit
GAGGTATA20106570.4988764754834794No Hit
GAGGTGCG20076890.4981400667478099No Hit
GCCATGCG20016120.4966322648992037No Hit
TAGCTGGT19988000.495934562283064No Hit
AAGCAGGA19689460.48852730271612455No Hit
TCGTGAAG19327600.4795489716821167No Hit
CTGTAGCA19036050.4723151453046088No Hit
ATGTGCAT18896910.4688628571819319No Hit
CACATTAT18748060.4651696482767971No Hit
AGTGGATG18132630.4498998360061414No Hit
CACCACGG18042280.44765810658337396No Hit
CGACCTCG17832500.44245312597121966No Hit
AATATTCG16630740.4126355194288295No Hit
GAGACTGA16352360.4057284620219674TruSeq Adapter, Index 15 (100% over 8bp)
AGCGAAGA16099430.39945286021897286No Hit
TATGGTGG16059270.3984564257572314No Hit
TATATAGC15999510.3969736836398592No Hit
CGCGTCGG15495710.38447359196094144No Hit
GGACACAT14063670.34894236669719125No Hit
CGAGCCTT13299470.3299813304506074No Hit
GATCTTCC10668490.26470246739900166No Hit
TGACGACC10054050.24945721862727832No Hit
CGTGCAGG8572140.2126886381193288No Hit
GTGGACAC8240100.20445018944710205No Hit
TGGCGCGA7326890.18179197443697012No Hit
GCGCAATC5747250.14259855478693914No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)