FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21793508
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC2403551.1028743055041894No Hit
TTCCAT2363751.08461198628509No Hit
GGCTAC2263661.0386854654147464No Hit
GACTAA1950800.89512895308089No Hit
TGCGAC1929180.8852085676156405No Hit
CGTCAG1926890.8841577959821797No Hit
GAAGAT1847260.8476193919767299No Hit
GCTGTC1844270.846247423774089No Hit
TCTTCA1840760.8446368524057715No Hit
CTATTA1784250.8187071122281002No Hit
AGTCAA1679050.7704358564027416No Hit
AATCGG1672770.767554264324954No Hit
CTACCG1672680.7675129676232023No Hit
TTCACA1660860.7620893341264747No Hit
TACGAA1651390.7577440033977091No Hit
CATAAC1648020.7561976713432276No Hit
AGTGCA1644760.7547018130353313No Hit
ACAGTG1619050.7429047219015865No Hit
ATCCAC1607050.7373984950013555No Hit
AGTTCC1600480.734383835773479No Hit
ACCAGG1599400.7338882753524582No Hit
GTACGC1595710.7321951105806371No Hit
ACTTGA1591610.7303138163897249No Hit
TATTCT1577650.7239082390957895No Hit
TAGCTT1515140.6952253854680026No Hit
AGATGC1475270.676930946591985No Hit
GCCAAT1471830.6753524948805856No Hit
AACATA1445270.6631653793414075No Hit
TGACCA1437360.659535858109672No Hit
TTGAAT1435390.6586319191935507No Hit
ACCGCT1431120.6566726201215518No Hit
ACTAAT1418140.6507167180244686No Hit
CAAGCT1411670.6477479440207606No Hit
CGATGT1409970.6469678952098946No Hit
GGATTC1401090.6428932873037236No Hit
AGAACG1364640.626168123094272No Hit
TAAGTC1355660.6220476299639324No Hit
GTTGGC1353900.6212400500185652No Hit
CTTAGC1346800.6179821991025952No Hit
TGCGGA1344620.6169819012157198No Hit
GTAAGA1336520.6132651980580639No Hit
GAACCG1326370.6086078478049518No Hit
CAGATC1316370.6040193253880926No Hit
TATGGT1308680.6004907516495279No Hit
GGAGGC1300920.5969300582540452No Hit
TACTCG1294800.5941218825349274No Hit
TCGTGG1287620.5908273234396225No Hit
GTCTCA1285160.5896985469250752No Hit
AGGCTT1282360.5884137606483546No Hit
AAGCAT1282360.5884137606483546No Hit
GCTCAA1279940.5873033382234746No Hit
GATCAG1278470.5866288254281964No Hit
ACCTGC1276410.5856835898103233No Hit
TCCAGA1275210.5851329671203003No Hit
GCACAT1271010.5832057877052194No Hit
ATGTGA1265220.5805490332258579No Hit
TCGGAC1258990.5776903837601546No Hit
CGAAGC1251480.5742444034250934No Hit
AAGTTG1245410.5714591703180598No Hit
CACCTA1232250.5654206748174732No Hit
GCATAA1229000.563929405031994No Hit
GACCGA1223740.5615158422407259No Hit
GACCAT1191510.5467270344911889No Hit
AGGTGT1191310.5466352640428517No Hit
TGGCAT1186850.5445887830449325No Hit
ATTCGA1185040.543758260487481No Hit
TAATGA1184910.5436986096960618No Hit
TTAGTA1182300.5425010053452616No Hit
TCTCTG1178270.5406518308112672No Hit
GACACG1175630.5394404608932164No Hit
CGTGAA1154130.5295751376969692No Hit
CAGCCT1153990.5295108983831333No Hit
CATGAG1153140.5291208739777001No Hit
CGAATT1151920.5285610742428434No Hit
TTAGGC1147190.5263907031396688No Hit
CAGGAC1145560.5256427739857209No Hit
ATACCT1140460.5233026275531227No Hit
GGCGTT1137940.5221463199040742No Hit
TCCACG1119770.513808974672641No Hit
CTCCGC1097000.5033609091294527No Hit
CCTGCT1095840.502828640529097No Hit
GTCTGC1095350.5026038029306709No Hit
ACACGA1094440.5021862473907368No Hit
CGTACT1092230.5011721839366108No Hit
CCTGAC1090960.5005894415896698No Hit
GTTACA1090360.5003141302446582No Hit
GAGATA1089220.4997910386891362No Hit
ATTGCT1088690.49954784700104266No Hit
CTATGC1085430.4980519886931466No Hit
GGCCAG1083840.49732241362886603No Hit
TCAAGC1081660.4963221157419907No Hit
CGAGCC1081230.4961248092780658No Hit
TGTGTG1064140.48828302446765337No Hit
AGTAGG1059730.48625948608181846No Hit
CATTAA1047250.48053301010557825No Hit
CGAGAG1044550.4792941090530262No Hit
GAGAAG1042190.4782112177626475No Hit
CCATGA1041900.47807815061255854No Hit
CCTTAT1039110.4767979528582549No Hit
GGACTA1037160.4759031909869673No Hit
AATGTA1035370.4750818454743495No Hit
GATCCT1034560.4747101751585839No Hit
GCGCAG1032670.47384294442179753No Hit
GCCATA1032250.4736502264802894No Hit
CAGTCC1023370.4695756185741185No Hit
ACGCTA1022040.46896534509267623No Hit
TTACGT1020670.46833671752156647No Hit
TGTGAT1016180.46627647095639674No Hit
TATTGG1009890.4633902903561923No Hit
AGACGG1002120.45982500843829277No Hit
AACAAT1001250.459425806988026No Hit
AGAGGT998170.45801254208363334No Hit
AGCCTA996220.4571177802123458No Hit
TATAAG990790.45462621253999125No Hit
CTAGTT989010.4538094555497903No Hit
CTCGTC988490.45357085238411365No Hit
TTCTGT987130.4529468133354208No Hit
CTTCAC985050.4519924006727141No Hit
AGTTAG982110.45064337508215746No Hit
TCCTCC981730.4504690112303168No Hit
CCGACT981400.4503175899905605No Hit
TTCTAC974810.4472937537178503No Hit
GCCTGG973250.44657794422082026No Hit
GAGCCA971960.4459860248290455No Hit
CCACTT969780.4449857269421701No Hit
ACTAGC966470.44346692602218973No Hit
ACAGCC963360.44203989555054657No Hit
CACATT948610.43527182498567923No Hit
GCGGCT942100.4322846968923039No Hit
TGCATA936270.42960958832327495No Hit
AGGACT921240.4227130391307356No Hit
CCGGAA919120.4217402723783615No Hit
GAGTGA918780.42158426261618825No Hit
GATTCA917380.42094186947782797No Hit
AATTGC913820.4193083554974261No Hit
CGACAA911360.4181795789828788No Hit
CCGCTC908390.4168167878250716No Hit
ATGGCC906200.4158119014157794No Hit
GGATCG880820.40416623152179076No Hit
TTAGCG878150.4029410960364894No Hit
AAGGCG877680.402725435482897No Hit
AACTAG877350.40257401424314065No Hit
ACGTAA873550.4008303757247342No Hit
TGACAC871900.40007326952595246No Hit
ATCAGC868300.3984214014558831No Hit
GGTGGT863550.396241853307875No Hit
GACTTG858030.3937089889337687No Hit
AACCTT856280.3929059975108184No Hit
GCGTAT845250.3878448572850227No Hit
GAGTCG843380.38698680359307003No Hit
CTGGAG838940.38494949963998454No Hit
CAACGG834240.3827928941040607No Hit
TTCGTT818680.37565315322342785No Hit
ATTCAT816730.3747583913521403No Hit
GAGCAC814630.3737948016445999No Hit
CGTTGC805980.3698257297540167No Hit
TAGAGC800900.36749476036625217No Hit
TCATGT799980.36707261630390114No Hit
TGATAA793510.36410384230019327No Hit
AGGTCA792910.3638285309551817No Hit
TCAATG791550.36320449190648885No Hit
GCTTCT789940.36246573979737456No Hit
TCTCGC784970.3601852441561955No Hit
CGCTTG781180.35844619416020584No Hit
GTACTT764800.35093019444139056No Hit
CAGTAG742250.3405830763913731No Hit
CCGTCG740750.3398947980288442No Hit
GAATAC739630.339380883518156No Hit
AAGAGG731550.3356733574053337No Hit
AACTCT731400.3356045295690808No Hit
GGCAGG724780.33256692772912005No Hit
TACGGC720440.3305755090002032No Hit
ATTGAA720180.33045620741736487No Hit
GTGGTG714150.32768932839999876No Hit
CTGTAT704970.323477064821322No Hit
CAAGGA703000.32257312590520076No Hit
CCTCGG690890.3170164252583843No Hit
ACGAAC684080.31389164149250315No Hit
GGCCGT681140.3125426159019466No Hit
GGTAAT679220.3116616195979096No Hit
CTGTTC678370.3112715951924766No Hit
CTGAGG661670.30360876275632176No Hit
TACAGT650490.2984787946942732No Hit
ATCCGG625310.28692489524862175No Hit
CATACG614580.2820014106953318No Hit
CCAGCG613150.28134525198972093No Hit
ATGCCG601100.2758160824774057No Hit
CGTCTC598870.27479284197844606No Hit
AAGCGC533770.24492156104469276No Hit
GTCGAG406640.18658767555916192No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)