FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27280774
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAC3793001.3903564466316096No Hit
GACTAA3194551.1709895034503053No Hit
ACCGCT3167751.1611657352536993No Hit
TTCCAT2539030.9307030658294373No Hit
ATACCT2411070.8837982382757908No Hit
GACCAT2398120.8790513055091473No Hit
CTTCAC2347020.8603201654029318No Hit
AAGCAT2256880.8272785808789737No Hit
TCGGAC2250730.8250242460129613No Hit
TAAGTC2233540.8187231051435712No Hit
AGCCTA2232450.8183235563624405No Hit
ATTCAT2212690.8110803601100175No Hit
ATTGCT2196160.8050211478603942No Hit
CTATTA2179580.7989436076850311No Hit
TATTCT2135830.7829066726625864No Hit
ATTCGA2111460.7739736416569413No Hit
AGTGCA2085130.7643221559622905No Hit
CGAGAG2059100.7547806378220794No Hit
AACATA2051770.7520937639086046No Hit
GGAGGC2048240.7507998123513651No Hit
AGATGC1996290.7317570975075708No Hit
TACTCG1935880.7096132976285789No Hit
TTCTGT1912930.701200779713948No Hit
TGACAC1908370.6995292728864657No Hit
GCCAAT1904380.6980667044124188No Hit
TGACCA1881560.6897018391047116No Hit
TGATAA1871740.686102234489388No Hit
TTGAAT1863280.6830011494541907No Hit
GGTGGT1827660.6699443351570596No Hit
GATCAG1822080.6678989386444827No Hit
TCTCGC1816370.6658058895249819No Hit
GTACGC1812600.6644239639241908No Hit
CTCCGC1794150.657660959326154No Hit
TATGGT1785060.6543289424266334No Hit
TAATGA1783960.6539257280603549No Hit
GGCGTT1782160.6532659227337171No Hit
TCTTCA1766930.6476832365533324No Hit
GTTACA1761800.6458027913724149No Hit
CGATGT1753030.6425880731976299No Hit
GATTCA1733090.6352788964125431No Hit
CGTCAG1727260.6331418602712665No Hit
AGTCAA1705780.6252681833733896No Hit
AACCTT1699290.6228892186123458No Hit
TGTGTG1695140.621368000775931No Hit
ACCTGC1686770.6182999060070656No Hit
GTAAGA1686180.6180836364833343No Hit
GGATTC1674580.6138315577116691No Hit
AGGTGT1673740.6135236485592381No Hit
AGACGG1669140.6118374793911638No Hit
AATCGG1621090.5942243427550846No Hit
TCAAGC1615370.5921276280504358No Hit
GACCGA1606430.5888505949281351No Hit
GCACAT1602940.5875713057114875No Hit
TTCACA1597930.5857348475523458No Hit
CTAGTT1597820.5856945261157179No Hit
TGCGAC1593610.5841513147684153No Hit
AATTGC1586720.5816257266014521No Hit
CATTAA1577310.5781764109771959No Hit
TCCTCC1573190.5766661898962251No Hit
ATCCAC1561940.5725424066047393No Hit
ACGCTA1554190.5697015781150492No Hit
GCATAA1552910.5692323832161067No Hit
CATAAC1550690.5684186233132535No Hit
GGATCG1543160.565658437696819No Hit
CACCTA1543070.565625447430487No Hit
CCGACT1541080.5648959959860377No Hit
AGAACG1539620.5643608205544315No Hit
GTCTCA1539530.5643278302880996No Hit
TATTGG1538300.5638769633148971No Hit
GAACCG1532780.5618535603132082No Hit
ACTTGA1521160.5575941503712467No Hit
CAGCCT1505010.5516742303572472No Hit
CAAGCT1500010.5498414377832536No Hit
GAGCAC1495340.5481296095191434No Hit
TGCGGA1483330.5437272417564105No Hit
AACTAG1482230.543324027390132No Hit
TCGTGG1481040.5428878227575215No Hit
GGTACC1472970.5399296955430957No Hit
TCCAGA1472000.5395741337837409No Hit
CGAATT1468590.5383241692482772No Hit
TTCGTT1464950.5369898962544097No Hit
GATCCT1463870.5365940130584271No Hit
ACGTAA1457060.5340977495726478No Hit
GTACTT1454090.5330090707836955No Hit
TCTCTG1444240.5293984694129279No Hit
ATGTGA1429950.524160348236454No Hit
GCTCAA1415440.5188415841867243No Hit
ATCCGG1411860.5175293047037449No Hit
CGTACT1411640.5174486618304891No Hit
AACAAT1406030.5153922685624682No Hit
ACTAGC1401640.5137830766825018No Hit
GCGTAT1398590.5126650732123655No Hit
GCGGCT1398360.5125807647539619No Hit
TGTGAT1385420.5078374975724662No Hit
AGTAGG1381670.506462903141971No Hit
AAGTTG1378210.5051946106807673No Hit
AGGTCA1377690.505004000253072No Hit
GCCTGG1371120.5025957108108443No Hit
ACCAGG1357730.4976874922976892No Hit
ACAGTG1355350.49681508303246824No Hit
TTAGTA1352570.4957960503613277No Hit
GAGCCA1348270.49421984874769315No Hit
TTACGT1326490.4862362042953767No Hit
CGACAA1292450.4737585524516277No Hit
GCTGTC1272900.4665923334873124No Hit
GAGTCG1268290.4649024987340902No Hit
CCGCTC1264970.4636855244649583No Hit
TAGAGC1264760.4636085471768506No Hit
CATGAG1264350.4634582581857832No Hit
GAGAAG1261960.4625821833354141No Hit
CGTCTC1253990.4596607119724682No Hit
TCAATG1230360.4509989342677741No Hit
TACAGT1219470.4470071120416158No Hit
TGGCAT1218150.4465232548020815No Hit
GAGTGA1212470.4444412024380247No Hit
AGGCTT1207700.4426927183224347No Hit
CCGTCG1207370.4425717540125511No Hit
TTAGGC1197620.4389978084932634No Hit
AGGACT1186320.4348556972760377No Hit
CCTTAT1184450.4341702328533641No Hit
CCACTT1178110.43184625186954No Hit
CAGATC1171190.4293096669471328No Hit
CAGTCC1170030.4288844590699663No Hit
AAGCGC1159650.4250795816863554No Hit
CCATGA1156930.4240825425261028No Hit
CCTGCT1153110.42268228899957166No Hit
ACACGA1150000.42154229201854754No Hit
GACACG1149310.4212893666433365No Hit
ATCAGC1148310.42092280812853766No Hit
TTCTAC1138530.417337865853806No Hit
CGCTTG1102960.404299379482415No Hit
TTAGCG1085690.3979689139318408No Hit
CTGAGG1079070.39554229656387313No Hit
CAGTAG1077670.39502911464315493No Hit
GGCAGG1074990.3940467378234943No Hit
CCTGAC1072430.39310834802560957No Hit
TACGAA1071100.3926208252009272No Hit
CTATGC1068820.3917850717871861No Hit
AGTTAG1065770.39066706831704995No Hit
ACTAAT1056160.3871444409898341No Hit
GGTAAT1054650.386590937632488No Hit
TACGGC1031630.37815276062182107No Hit
CTACCG1015400.37220351592663753No Hit
AAGAGG1011460.37075927537833053No Hit
TCATGT1009180.36992352196458944No Hit
GGCCGT1008490.3696705965893783No Hit
GCGCAG1006600.3689778009964087No Hit
CAACGG996760.3653708652107891No Hit
AGAGGT995210.3648026995128511No Hit
GAAGAT989730.362793958851754No Hit
GAATAC989700.36278296209631006No Hit
CTTAGC989500.3627096503933503No Hit
GCTTCT987060.3618152476172414No Hit
CTCGTC985570.36126907543019127No Hit
CCAGCG963620.35322311603035894No Hit
GTGGTG955360.35019534269812136No Hit
CATACG939760.34447702986726114No Hit
AATGTA930620.34112668504200067No Hit
ACAGCC923650.3385717721938534No Hit
ATGCCG911050.33395313490738937No Hit
CTGTAT901380.33040851406928556No Hit
GTCTGC895200.32814318244782936No Hit
CACATT890780.32652299381241895No Hit
GGACTA887370.3252730292769553No Hit
TATAAG887200.3252107143294395No Hit
TAGCTT885640.32463888304635347No Hit
AGTTCC883480.32384711665438815No Hit
CCTCGG880790.32286107424957955No Hit
CAAGGA869880.31886192085312537No Hit
GAGATA862370.3161090664069868No Hit
AACTCT855820.3137081081350551No Hit
CGAGCC849230.31129248752253147No Hit
GGCCAG845280.30984458138907645No Hit
CTGGAG837210.30688645417465066No Hit
GTCGAG836980.3068021457162469No Hit
CAGGAC795370.2915496459154715No Hit
CGTGAA790160.28963987605337005No Hit
TCCACG732870.2686397387405504No Hit
GCCATA732020.26832816400297144No Hit
AAGGCG722210.26473222497279586No Hit
GTTGGC716400.26260252000181517No Hit
CCGGAA709940.26023455199621537No Hit
ATGGCC704920.2583944282519257No Hit
TGCATA703560.25789590867179946No Hit
CGTTGC697120.25553527183649555No Hit
ATTGAA674580.247273042912932No Hit
CTGTTC655620.24032309347234798No Hit
GACTTG569070.20859745401651725No Hit
ACGAAC543990.19940416646536496No Hit
CGAAGC416680.15273760194633773No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)