FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22057575
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAAC6584352.9850742885380646No Hit
ATTGCT5017682.2748103542660516No Hit
TGTGTG4744972.1511748231616576No Hit
CAAGCT4077441.8485440942624018No Hit
AGATGC3931771.782503289686196No Hit
ACCTGC3632691.6469126819244635No Hit
AGGCTT3562641.6151548844331256No Hit
AATTGC3478561.5770364602636509No Hit
AAGTTG3360421.5234766287771888No Hit
CACCTA3316021.503347489467904No Hit
GCACAT3178661.4410740981272874No Hit
TTCTAC3101471.4060793174226993No Hit
CCTGCT2944641.3349790264795653No Hit
TTAGTA2806381.2722976120448417No Hit
GACCGA2801611.270135089646074No Hit
CGCTTG2628261.191545308131107No Hit
GAGCAC2398011.0871593998887004No Hit
GATTCA2385011.0812657329738196No Hit
ACTAGC2378791.078445840034546No Hit
GTGGTG2332531.0574734529974397No Hit
ATTCGA2317281.0505597283472912No Hit
TCGTGG2282761.0349097758933157No Hit
TTCGTT2272051.0300543010734406No Hit
CCATGA2265021.0268671873494708No Hit
CATTAA2235871.0136517726903342No Hit
TACTCG2184250.9902493814483233No Hit
TGCGAC2087890.9465637088392537No Hit
AATGTA2076830.9415495583716704No Hit
GACTAA1963550.8901930515933869No Hit
TCATGT1959320.8882753430510834No Hit
CGTACT1890620.8571295802009061No Hit
CTTAGC1885860.8549715913920728No Hit
TTCACA1875820.8504198670978111No Hit
CTATTA1816830.8236762200740562No Hit
TCTTCA1734800.786487181841159No Hit
GCCTGG1730530.7845513389391172No Hit
GGTACC1722100.7807295226243138No Hit
CTCCGC1660990.7530247545344401No Hit
AATCGG1658580.7519321593602197No Hit
TCCAGA1644710.7456440701210355No Hit
CCAGCG1636110.7417451827773451No Hit
AAGAGG1631960.7398637429545178No Hit
TGTGAT1602340.7264352495684588No Hit
AGAACG1584130.7181795822976913No Hit
GTCGAG1574470.7138001344209416No Hit
AGGTCA1548810.702166942648954No Hit
ATGTGA1535260.6960239282876743No Hit
AGGTGT1522770.6903614744594544No Hit
AGGACT1504770.6822010125773118No Hit
TCAATG1485090.6732789075861694No Hit
AGAGGT1476260.669275747673985No Hit
CGTTGC1463500.6634908869175329No Hit
AAGCGC1452990.6587260838963486No Hit
GGTAAT1439020.652392658757819No Hit
GGCCGT1432410.649395955811099No Hit
CGTCTC1422640.6449666384450693No Hit
GCTTCT1400230.6348068634018019No Hit
CCACTT1350450.6122386527077432No Hit
GCGCAG1331840.6038016418395947No Hit
CTACCG1288060.5839535851062503No Hit
ACCGCT1281210.5808480760011017No Hit
GCTGTC1245410.5646178240355071No Hit
AGTCAA1185410.5374162844283653No Hit
CTGAGG1181260.535534844605538No Hit
TATTCT1149840.5212903050312647No Hit
AACTAG1135830.5149387455329971No Hit
GCGTAT1107910.5022809624358072No Hit
TCTCGC1101870.4995426741153549No Hit
GAGCCA1086340.49250200894703977No Hit
ATCCAC1053470.47760009883226057No Hit
GCATAA1053460.47759556524232605No Hit
TACGGC1051010.47648483570836775No Hit
TCCTCC1027060.4656268878151837No Hit
GCCAAT1025830.46506925625323725No Hit
GAGATA1024940.4646657667490646No Hit
GCTCAA1023580.4640491985179695No Hit
GTAAGA1023440.4639857282588861No Hit
ACCAGG1020410.46261205050872545No Hit
TATTGG998500.4526789549621842No Hit
CCGGAA983740.4459873762188273No Hit
TCTCTG970760.4401027764838156No Hit
CGAGAG966140.4380082579340657No Hit
GATCCT954150.4325724836025719No Hit
GGATCG950780.43104466379463746No Hit
GGATTC921820.41791538734425704No Hit
GTTACA899260.40768760845197166No Hit
CCGCTC879330.3986521637124661No Hit
AACATA877770.3979449236826804No Hit
GAAGAT873330.39593200975175197No Hit
AGTAGG871930.39529730716091865No Hit
CGAATT863420.39143922212663906No Hit
ACAGCC855040.3876400737615082No Hit
CGATGT845940.38351450692109174No Hit
TGCGGA842010.3817328060768239No Hit
ACAGTG823010.37311898520122905No Hit
GGACTA818480.37106526896088987No Hit
TTACGT815550.3697369271100744No Hit
TAATGA814370.36920196349780066No Hit
TGACCA807830.3662369956806222No Hit
GGCTAC804580.36476357895190203No Hit
CAACGG802640.3638840625046044No Hit
ACACGA771950.3499704749955514No Hit
CACATT762840.3458403745652004No Hit
CATGAG755490.3425081859633255No Hit
CTGGAG749950.33999657713959947No Hit
GACACG747670.33896291863452804No Hit
TAAGTC740970.3359254133783972No Hit
TATGGT734920.33318259146801044No Hit
ACGTAA718830.3258880452633619No Hit
CAGATC716760.3249495921469155No Hit
AAGCAT715030.32416528108824294No Hit
TTAGGC713520.32348070900812986No Hit
GAGTGA698440.31664405538686824No Hit
TCAAGC694310.31477168274391No Hit
CCTCGG672010.30466177718992227No Hit
TGGCAT671810.30457110539123183No Hit
GAGTCG649350.29438866239829176No Hit
CAGTCC627920.28467317916860757No Hit
ACTTGA608840.27602308957353655No Hit
TAGCTT604390.2740056420526735No Hit
AGTGCA599420.27175244785521524No Hit
CGTCAG598070.2711404132140546No Hit
GTACGC590720.2678082246121797No Hit
GATCAG580140.26301168646145373No Hit
GAGAAG551660.2501000223279304No Hit
CGAGCC543970.24661369166828176No Hit
ACGCTA540660.24511307339995445No Hit
TCCACG536660.24329963742614497No Hit
CAGGAC534140.24215717276264503No Hit
CTATGC531470.24094670425012724No Hit
CCGTCG511880.23206540156839542No Hit
GGAGGC509780.23111334768214548No Hit
TTCCAT508330.23045597714163954No Hit
GCGGCT506540.22964446454335985No Hit
TCGGAC483910.21938495052153287No Hit
GTTGGC475670.2156492724154854No Hit
TTGAAT472790.2143435985143426No Hit
TGCATA465310.2109524732433189No Hit
GAACCG462630.20973747114086658No Hit
AGACGG461580.20926144419774162No Hit
CTCGTC445680.20205303620184903No Hit
AACCTT437190.19820401834743845No Hit
AGTTAG431910.19581028286201No Hit
TACGAA408030.1849840700983676No Hit
CGACAA397850.1803688755450225No Hit
TTCTGT397680.1802918045161356No Hit
ATCCGG385790.17490136608398701No Hit
CTGTTC382880.17358209141304065No Hit
AGCCTA382270.1733055424270347No Hit
ATCAGC374660.16985548048686222No Hit
GGCGTT370560.16799670861370755No Hit
CCGACT367580.1666456988132195No Hit
CATACG367390.16655956060446356No Hit
CGTGAA356890.16179929117321373No Hit
TATAAG350650.158970331054071No Hit
ACGAAC349380.15839456513238648No Hit
CAGCCT344930.15637711761152348No Hit
ACTAAT343080.1555384034736366No Hit
ATACCT339390.1538655087877974No Hit
CGAAGC323130.14649389155426196No Hit
AAGGCG314370.14252246677161928No Hit
GTCTCA312230.14155227852563124No Hit
GGTGGT307020.1391902781697444No Hit
CTAGTT304050.13784380195919088No Hit
CCTTAT299190.13564047725101241No Hit
GACTTG298390.1352777900562505No Hit
AGTTCC295740.13407638872360175No Hit
GACCAT286360.1298238813650186No Hit
CTGTAT280540.12718533202312585No Hit
AACAAT279730.12681811123842943No Hit
GAATAC279370.12665490200078658No Hit
GCCATA277410.12576631837361996No Hit
ATGGCC259660.1177191962398405No Hit
ATTCAT257340.11666740337503102No Hit
ATGCCG253890.11510331484762036No Hit
TAGAGC248240.11254183653461453No Hit
TGATAA230280.1043995090122101No Hit
GGCCAG224370.10172015736090662No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)