FastQCFastQC Report
Thu 8 Sep 2022
EGAF00001836160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28420621
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTG6693392.3551174339223624No Hit
CGTGAA6482672.2809740856823644No Hit
GGAGGC5186601.8249425302846127No Hit
GAGAAG5068481.7833811583497772No Hit
TAAGTC4768881.6779647425719517No Hit
AATCGG4638041.6319277471101001No Hit
TATTCT4575451.6099050052424964No Hit
GTACGC4556791.6033393499740909No Hit
CGTCAG4385491.5430662123814958No Hit
TTACGT4326731.522391083572734No Hit
CAGCCT4291331.5099353388513221No Hit
TTGAAT4271721.5030354192471727No Hit
CTCGTC3814301.342088900872363No Hit
ACGCTA3776061.328633881715674No Hit
ATTCAT3709641.305263526789228No Hit
AAGGCG3685251.2966817297904927No Hit
ACCGCT3623711.2750284379781849No Hit
AAGCAT3595511.2651060650645176No Hit
CTATTA3510721.2352720934563675No Hit
TCAAGC3481681.2250541604984633No Hit
GGATTC3435481.2087983580654342No Hit
TGGCAT3332381.1725218812073108No Hit
TAATGA3110141.094325138074921No Hit
CACATT3045711.0716549789675602No Hit
CATACG2873751.0111496156259216No Hit
GAGATA2857681.0054952704939135No Hit
TGATAA2843231.0004109340186478No Hit
ATCCAC2781390.9786520850476842No Hit
GTAAGA2776730.9770124305165604No Hit
TAGAGC2675870.941524113776402No Hit
TCCACG2609740.918255797436657No Hit
AGTCAA2605890.9169011472339046No Hit
CTGGAG2568940.9039000238594365No Hit
CTATGC2494650.8777605528042474No Hit
AACCTT2488100.8754558881735905No Hit
CGAATT2453990.8634540392343996No Hit
ACGAAC2385900.8394960827914352No Hit
CTGTTC2339280.8230925003362876No Hit
CGAGCC2304120.8107212013418004No Hit
GAAGAT2269790.798641943819595No Hit
GATCCT2241500.7886879037583309No Hit
CTTCAC2214730.7792686866342576No Hit
CCTGCT2209320.7773651392064937No Hit
GGTACC2087100.7343611527700257No Hit
TGCATA2025650.7127395281053148No Hit
AGTTCC2024950.7124932280684507No Hit
GGACTA1985190.6985033859745711No Hit
CGAGAG1975350.6950411111706531No Hit
ATCCGG1876820.6603726216960566No Hit
ATACCT1855370.6528252848521501No Hit
CACCTA1777510.6254296836089542No Hit
ATCAGC1755420.617657158159915No Hit
GGATCG1753760.6170730752153516No Hit
TTAGCG1738940.6118585515777435No Hit
GACCAT1715410.6035793517671553No Hit
CCTGAC1621030.5703710696539671No Hit
TTCTGT1607230.5655154403557895No Hit
GCATAA1602920.5639989358430979No Hit
TACGGC1599780.5628941042491648No Hit
ACTAAT1577320.554991391637783No Hit
CAGTAG1561080.5492772307825364No Hit
ACAGCC1548340.5447945701116101No Hit
CCGACT1524750.5364942588692907No Hit
GTCTGC1493620.5255409443727497No Hit
GTTACA1480690.5209914308346746No Hit
CCATGA1475330.5191054762666868No Hit
AGACGG1474860.5189401033847924No Hit
CAGGAC1454720.5118536994670173No Hit
AGTAGG1441800.5073077045008975No Hit
CTAGTT1431640.503732835394413No Hit
ACACGA1430230.5032367167487297No Hit
GGTGGT1421200.5000594462731831No Hit
CCTTAT1414930.497853301657272No Hit
TATAAG1362320.479342094600959No Hit
TCGTGG1353980.47640760559032114No Hit
GCACAT1330820.46825859294207545No Hit
AACTAG1320840.4647470581307847No Hit
CCTCGG1317520.4635788922416579No Hit
AACTCT1307570.4600779131462328No Hit
TTAGTA1286550.45268187489640005No Hit
GACTAA1259070.44301283916350737No Hit
TATTGG1258020.4426433891082112No Hit
GTACTT1257970.44262579624843523No Hit
ATGGCC1241740.4369151539651438No Hit
TATGGT1241040.43666885392827975No Hit
GAGCCA1237160.4353036480096617No Hit
ATTGAA1211270.426194065217646No Hit
GTCTCA1171890.41233792885806403No Hit
AGATGC1160140.40820360681070267No Hit
GGCAGG1145100.4029116745900802No Hit
TGACAC1143050.40219036733926394No Hit
AGGCTT1140840.40141276293716455No Hit
AGCCTA1130360.39772529952811375No Hit
GAATAC1118010.3933798631634404No Hit
CAAGGA1104020.388457380998114No Hit
GGCCAG1083260.3811528256191165No Hit
CATAAC1034640.3640455287729287No Hit
CTGTAT1027390.3614945641054078No Hit
GAGTCG1018950.3585248893752181No Hit
TACAGT989420.3481345463915092No Hit
CCGCTC986650.34715990195991847No Hit
GATTCA982340.3456433974472268No Hit
ACGTAA964970.33953163796104247No Hit
GAGTGA947240.33329320988447086No Hit
TTCACA928140.32657273745003673No Hit
GGCGTT910190.3202569007904507No Hit
TGCGGA893100.314243661319012No Hit
CAAGCT875350.30799819609852996No Hit
AATTGC842460.2964256129378735No Hit
AACAAT841040.29592597572023494No Hit
CAACGG821970.2892160590016664No Hit
ACCTGC811240.28544063129373565No Hit
ATGCCG803570.2827418866040964No Hit
CGTCTC797410.28057444627969247No Hit
GCCTGG761170.26782314151404363No Hit
GTTGGC750900.2642095681160521No Hit
GTGGTG744310.26189082919757456No Hit
CCACTT713470.2510395532877343No Hit
GCGTAT706300.24851673719585507No Hit
CGCTTG676970.2381967656512502No Hit
TCCAGA657520.23135314319838404No Hit
AAGAGG652560.22960793150860426No Hit
GAGCAC639490.2250091579631564No Hit
TGTGTG637460.22429488785625057No Hit
CGTTGC636690.2240239578157001No Hit
TCCTCC617160.21715218678719228No Hit
TGTGAT616000.21674403244038898No Hit
ATTCGA602490.21199044172891227No Hit
ATTGCT588450.20705036670380988No Hit
AAGTTG582850.20507996640889725No Hit
CTTAGC562550.19793726533983896No Hit
ACCAGG547990.19281422457306616No Hit
GCGGCT518830.1825540687516997No Hit
GCTGTC514290.1809566370840384No Hit
TACTCG499810.17586174489290715No Hit
GGCTAC497230.17495395332846528No Hit
CATTAA484340.17041851407821104No Hit
GACACG481850.16954238966136595No Hit
GGCCGT464490.1634341487471368No Hit
AACATA458960.1614883784559106No Hit
GCGCAG456920.16077058977704956No Hit
ACTAGC447200.15735053783659408No Hit
ATGTGA445570.15677701060789628No Hit
TCATGT444350.15634774482936176No Hit
AGAACG442590.15572847616524635No Hit
AGGTCA431590.1518580470145251No Hit
AAGCGC431460.1518123055791075No Hit
GGTAAT424700.14943375093739156No Hit
TTCGTT423750.14909948660164746No Hit
GCCAAT420900.14809669359441513No Hit
GACCGA419520.14761113066459738No Hit
GCTCAA417000.1467244505318867No Hit
TGCGAC414420.1458166589674448No Hit
CATGAG411560.14481034738825727No Hit
TCAATG409460.14407144727766502No Hit
AATGTA400100.1407780639275968No Hit
NNNNNN394130.13867747647034173No Hit
CTACCG393560.13847691786889527No Hit
AGGACT391680.13781542634131744No Hit
AGGTGT390980.13756912630445337No Hit
TGACCA385640.13569020888037597No Hit
TCTTCA383520.13494427162587336No Hit
CTGAGG382710.13465926729750205No Hit
GCTTCT377550.13284368416861828No Hit
CTCCGC373850.13154181254519387No Hit
TTAGGC372490.13106328675928652No Hit
TAGCTT369020.12984234229083172No Hit
TTCTAC342560.12053220089736955No Hit
TCTCGC339040.11929366356913877No Hit
AGAGGT338400.11906847496400588No Hit
ACAGTG314060.11050427082504635No Hit
GATCAG313010.11013482076975024No Hit
CCGGAA300400.10569790153424163No Hit
GTCGAG297540.1046915899550541No Hit
CAGATC294310.10355509121352416No Hit
GAACCG284270.10002244497050222No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)