FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401164434
Sequences flagged as poor quality0
Sequence length6
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAT25790330.6428867520194973No Hit
GGCTAC25514080.6360005483437248No Hit
ACCGCT24679760.6152030915083564No Hit
GCCAAT24574380.612576238500744No Hit
TATGGT23314580.5811726569958093No Hit
AACCTT22963390.5724183914070509No Hit
CTGAGG22536670.5617813567191752No Hit
CTATTA21980830.5479256917376678No Hit
AAGCAT21683090.540503797502647No Hit
TACTCG21640250.5394359062249272No Hit
GCGTAT21469940.5351905149198745No Hit
CAACGG21385110.5330759206834372No Hit
GCATAA21345270.5320828117080787No Hit
CGTGAA21208480.5286729879947433No Hit
CATAAC21169570.5277030615331169No Hit
TTAGGC20963110.5225565434846101No Hit
CTACCG20749910.5172420145301315No Hit
ACTTGA20524210.5116158926491474No Hit
CCAGCG19723750.49166247873309726No Hit
TATTCT19152460.4774216848944291No Hit
AGGACT19075900.4755132405381679No Hit
CAAGGA19070850.4753873569958597No Hit
ACTAGC19007120.4737987316193638No Hit
ATTCGA18939550.47211438489584545No Hit
ACGCTA18849810.4698773969578769No Hit
CACCTA18740090.4671423588861818No Hit
TGGCAT18535460.46204145804211544No Hit
GGTGGT18493400.4609930101630096No Hit
AGCCTA18407750.4588579754305936No Hit
TGACCA18354960.4575420561833754No Hit
CGATGT18224500.4542900231280224No Hit
AATCGG18179940.45317925666361536No Hit
TAGAGC18154160.4525366274119903No Hit
CAAGCT18061670.4502310890301906No Hit
TTCTGT17957680.44763888515600564No Hit
GTCTCA17777820.4431554368551026No Hit
ATACCT17724670.4418305437316011No Hit
AATTGC17697720.4411587493820551No Hit
GCTGTC17693390.44105081359231363No Hit
CGTTGC17667220.4403984626413816No Hit
CTGTTC17440560.4347484104236419No Hit
AGATGC17303810.43133958380767123No Hit
CAGTAG17169030.4279798642369179No Hit
GTACTT17076540.4256743258551181No Hit
ATGGCC16988170.4234714885018945No Hit
TTAGTA16870780.42054525701049567No Hit
AACATA16789200.41851167693494984No Hit
AGGCTT16756500.41769654983921134No Hit
CGTACT16731990.4170855784289193No Hit
AGACGG16706680.4164546650713309No Hit
GGCCGT16695750.4161822082163944No Hit
ACCTGC16504470.41141408861783596No Hit
CTGTAT16485030.4109294992985345No Hit
TGTGTG16446580.40997103945660346No Hit
GAGAAG16400010.4088101688496144No Hit
AAGCGC16366880.40798432295720416No Hit
GACTAA16353070.40764007509200084No Hit
GACACG16318060.40676736562344407No Hit
AAGGCG16270770.4055885472638883No Hit
TGTGAT16211250.4041048663850395No Hit
CTAGTT16130700.4020969615666378No Hit
GAGTGA16086860.4010041428547976No Hit
CCACTT15990300.3985971498161275No Hit
CTCCGC15956570.39775634746324495No Hit
TCCTCC15805120.39398108756570377No Hit
CACATT15623670.3894580046445493No Hit
CCGCTC15459250.38535943592646604No Hit
ATTGCT15452550.38519242211785903No Hit
CCGACT15444620.3849947475652839No Hit
TTGAAT15373240.38321542731776664No Hit
CGAATT15297940.38133839152849724No Hit
TTACGT15290230.38114620101142865No Hit
GAGCAC15284970.3810150827079551No Hit
TGATAA15241420.3799294929520098No Hit
AGTTCC15121730.3769459283621339No Hit
AACAAT14845770.37006695364225634No Hit
GTAAGA14742080.36748222799830754No Hit
TTCTAC14737430.3673663154296475No Hit
GAGTCG14623340.3645223444708461No Hit
CTTAGC14576710.36335997821780985No Hit
CGCTTG14490330.3612067464584859No Hit
AAGTTG14423300.35953586054939257No Hit
GGTAAT14253160.3552947069081403No Hit
GGTACC14235060.35484352035056027No Hit
AACTCT14214100.3543210413313958No Hit
GCTTCT14202170.3540236570423389No Hit
ACAGTG14191770.3537644117274863No Hit
AGAACG14184640.35358667912220754No Hit
TAAGTC14061030.35050539899057953No Hit
AAGAGG14008930.3492066796729044No Hit
GTGGTG13959450.34797327023262487No Hit
TGCGGA13905280.34662295112632047No Hit
GAGATA13808090.34420025380415453No Hit
CTCGTC13786940.34367303857250714No Hit
CGAGAG13783380.34358429690703834No Hit
TCTCTG13781010.3435252188881729No Hit
GAATAC13744180.342607141489517No Hit
ACGAAC13734180.34235786714831257No Hit
GGCGTT13687990.34120646896628926No Hit
GCACAT13678630.34097314818292196No Hit
ATGCCG13520490.337031123751115No Hit
ACAGCC13456150.33542729263980564No Hit
TCATGT13455800.3354185680378635No Hit
CAGCCT13423630.33461665248220884No Hit
GATCAG13360370.3330397429997496No Hit
CCTGCT13333010.3323577284022142No Hit
AGTCAA13298150.3314887580487756No Hit
AATGTA13286010.3311861389985534No Hit
GAAGAT13248370.3302478703782599No Hit
TGACAC13180960.3285675120442008No Hit
TGCGAC13164120.3281477340536125No Hit
GGATTC13055490.3254398668851088No Hit
GTTACA13053570.32539200621159753No Hit
AGTAGG13044480.3251654158354427No Hit
CTGGAG12956010.322960085738807No Hit
AGGTGT12949490.32279755886834177No Hit
TACGGC12933110.3223892474974489No Hit
CGTCTC12914540.32192634504583223No Hit
CAGGAC12843110.32014577842660896No Hit
GGACTA12835560.3199575762989996No Hit
GTACGC12812280.3193772656326757No Hit
CCTCGG12698570.3165427670988401No Hit
TATTGG12681240.31611077466553283No Hit
GACCAT12680420.31609033416955407No Hit
TCAATG12671840.3158764567848006No Hit
ACGTAA12637240.3150139675642333No Hit
TAGCTT12581750.3136307442448899No Hit
CATTAA12550230.31284503152141346No Hit
CTATGC12475870.3109914275202173No Hit
TCGTGG12450120.3103495460916159No Hit
ATTCAT12406210.3092549824593872No Hit
GTTGGC12358020.30805372940912307No Hit
AACTAG12308960.3068307894911741No Hit
GGAGGC12277400.30604407967033287No Hit
CGAGCC12174840.3034875220269402No Hit
GGCCAG12073480.30096087730449206No Hit
TTCGTT12061750.30066847850225925No Hit
GCTCAA12047800.3003207407962791No Hit
TCAAGC12016380.29953752081621476No Hit
GACCGA11965960.298280679587862No Hit
TCTCGC11861730.29568249312948813No Hit
TGCATA11672010.2909532603281576No Hit
CATACG11498950.28663931857927366No Hit
ATCCAC11411750.284465646323971No Hit
ACACGA11214920.2795591794660441No Hit
ATTGAA11183990.27878817392869876No Hit
GTCGAG11169860.27843594928457693No Hit
TCTTCA11109360.2769278395202901No Hit
ATCCGG11085740.27633905352636523No Hit
CCATGA11027950.27489849710854475No Hit
GCGCAG10985070.2738296087334602No Hit
TTCACA10907930.27190670646540915No Hit
TAATGA10884590.271324900153038No Hit
TACAGT10849350.2704464573746336No Hit
ATGTGA10838880.2701854671393925No Hit
CGTCAG10833660.2700553459332838No Hit
GGATCG10807000.2693907805396328No Hit
TTAGCG10764750.26833759644804406No Hit
ACTAAT10657110.2656544074393195No Hit
GAGCCA10378380.25870638372692834No Hit
GCGGCT10374010.25859745083982194No Hit
ACCAGG10331280.2575323015798554No Hit
GATCCT10162600.25332754199241897No Hit
TATAAG10063110.2508475115717761No Hit
TCCAGA9841380.24532035160425014No Hit
CTTCAC9774800.24366068304051103No Hit
TCCACG9773940.24363924544716742No Hit
GCCTGG9640640.2403164184789123No Hit
CCTGAC9616900.23972464119289297No Hit
CCGGAA9562300.2383636032899168No Hit
GTCTGC9303300.2319073978527219No Hit
CAGATC8858200.2208121969257125No Hit
GATTCA8691840.21666526898543553No Hit
ATCAGC8618550.21483833733874821No Hit
ACTNGN8241630.20544268887007067No Hit
CATNAN8154070.20326004273848466No Hit
AGGTCA8002070.19947107275217724No Hit
CAANGN7888420.1966380698643888No Hit
CATGAG7848370.19563972612786507No Hit
AACNTN7816780.19485226848400025No Hit
GGCAGG7746310.1930956322015326No Hit
AGANGN7697220.19187194446056002No Hit
CTANTN7466810.18612841436486866No Hit
GGCNAN7334980.1828422307247706No Hit
TATNGN7065290.1761195510168282No Hit
TTANGN7019410.17497588033938222No Hit
CACNTN6957330.17342838522918508No Hit
CCTNAN6946170.17315019506440096No Hit
GCANAN6918900.17247042393593645No Hit
AATNGN6914920.17237121274813708No Hit
GCGNAN6534570.1628900631804264No Hit
CGTNAN6458490.16099358399254307No Hit
GAGNAN6305170.1571717097931967No Hit
AAGNGN6032520.15037524488025775No Hit
ATGNCN5976300.14897382453400643No Hit
CAGNAN5819250.14505897100539078No Hit
GACNAN5807710.14477130841564087No Hit
AGAGGT5756070.14348405571766115No Hit
CTGNAN5703650.1421773596210675No Hit
AGGNCN5607630.13978382739682252No Hit
TGANAN5588160.13929849025449748No Hit
GTANGN5486270.1367586339919655No Hit
ACGNAN5344760.13323115278958156No Hit
ACCNGN5335580.13300231894435588No Hit
AACNAN5330780.13288266726057774No Hit
TCCNCN5316600.13252919624474985No Hit
GAANAN5290610.13188133223195952No Hit
GCCNAN5208910.12984476086431931No Hit
GGCNGN5196640.12953890124766143No Hit
GAGNCN5091860.12692700470052137No Hit
GGANTN5081160.12666028115543265No Hit
TCANGN5049590.12587332206025023No Hit
CTGNGN4960780.12365951663601364No Hit
ACCNCN4946680.1233080398149154No Hit
CCGTCG4837800.12059394078788152No Hit
TACNGN4729790.11790152862853241No Hit
ATTNAN4611330.11494862478262466No Hit
CCGNCN4458570.11114070994638572No Hit
CGANGN4356670.10860060440951254No Hit
GCCATA4338130.10813844978091951No Hit
AAGNAN4258190.10614575069733126No Hit
TACNCN4226500.10535580031005441No Hit
CAGTCC4130170.10295454058123207No Hit
CTANCN4077770.10164834303332083No Hit
ATTNGN4042030.10075743653785621No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)