FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28484319
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA7308392.5657590760726983No Hit
TACTCG6987492.453100598964644No Hit
CTTAGC4987491.7509598877894887No Hit
GGTACC4579271.6076459472315274No Hit
AAGCGC4515401.585223083620149No Hit
GAAGAT4418941.5513588371201714No Hit
CTCCGC4411011.5485748492003617No Hit
CGAGAG4283971.503974871226516No Hit
GGTAAT4283601.5038449751949483No Hit
TATTGG4233511.486259861083567No Hit
GGCCGT4217041.4804777323270393No Hit
CAGGAC4127741.4491271495730687No Hit
CGAGCC4125611.448379369715667No Hit
ACACGA4010731.4080484072657662No Hit
CGTCTC3700021.2989673370811499No Hit
AGGTGT3605161.2656648031501123No Hit
GTCGAG3554331.2478198969755956No Hit
TCCAGA3550601.2465104045492539No Hit
TCCACG3524841.2374668321893179No Hit
TCTCGC3497221.227770268967989No Hit
GGATCG3453471.2124109409110324No Hit
GAGCCA3382191.18738664596475No Hit
AACCTT3282621.1524305706588946No Hit
ATACCT3237451.136572722697004No Hit
TTCTGT3156281.10807634193396No Hit
GATCCT3126451.097603913226783No Hit
TGATAA3051901.0714316182177288No Hit
AAGCAT2924621.0267473833585419No Hit
CAAGGA2918381.0245567043396753No Hit
GTCTCA2653070.9314142282987351No Hit
CTGTAT2627680.9225005519703665No Hit
CTAGTT2615830.9183403682566538No Hit
TAGAGC2554310.8967425199809059No Hit
ATTCAT2528170.8875655408858467No Hit
CTGTTC2441070.8569873129141687No Hit
ATTGAA2428860.8527007438724443No Hit
GTACTT2211790.7764939017850487No Hit
TGGCAT2188660.7683736444603082No Hit
GACCAT2135020.7495422305865904No Hit
ACGAAC2134270.7492789278198998No Hit
AACTCT2117640.7434406278064785No Hit
TATAAG2044320.7177001493347972No Hit
AGTTCC2041710.7167838557067135No Hit
TAAGTC2034910.7143965772887181No Hit
CTACCG2032180.7134381552179639No Hit
CCAGCG2009750.7055636471421346No Hit
ACTAAT2002150.702895512439669No Hit
TCAAGC1862490.6538650265783079No Hit
TGCATA1786370.6271415511109815No Hit
TGACAC1785660.6268922911585143No Hit
ATCAGC1774540.6229883888043803No Hit
AACATA1738510.6103393238925601No Hit
GTCTGC1729550.6071937335064953No Hit
CTATGC1720850.6041394214128833No Hit
CATACG1712830.601323837161071No Hit
ATCCGG1675930.5883693410398894No Hit
TAATGA1665680.5847708698951167No Hit
ACTAGC1641000.5761064535192153No Hit
GACACG1635220.5740772668639191No Hit
TACAGT1608860.5648230522906305No Hit
CGTACT1592620.5591216697158883No Hit
GCGTAT1574460.5527462320584179No Hit
AGAGGT1555640.5461390879662597No Hit
GCTGTC1493670.5243832580304973No Hit
GACTAA1491330.5235617533984225No Hit
AGGACT1489830.5230351478650411No Hit
AGAACG1464000.5139670005802139No Hit
TTAGCG1436250.5042247982126588No Hit
CTTCAC1414590.49662061431063176No Hit
TCCTCC1413000.49606241244524746No Hit
GAGCAC1309360.459677480792151No Hit
TCTTCA1293670.4541691869129818No Hit
CGTTGC1293060.4539550339960734No Hit
GCTCAA1287330.45194340085855655No Hit
TTCACA1234130.4332664579412974No Hit
CGTGAA1231750.432430910494999No Hit
TGCGAC1231220.4322448432065376No Hit
CCTGAC1202460.4221480597798389No Hit
TGCGGA1174240.4122408543451574No Hit
AAGTTG1159810.40717490911402865No Hit
CATAAC1135820.3987527312834827No Hit
ATGTGA1110670.3899233118404551No Hit
TCAATG1100830.3864687795414733No Hit
TGTGTG1100460.3863388835099059No Hit
GTTGGC1078340.3785732072443087No Hit
GCTTCT1073410.3768424303912619No Hit
CCGCTC1068680.3751818676093327No Hit
AATGTA1051200.3690451577936618No Hit
ATTCGA1048410.3680656715015725No Hit
GGCAGG1046190.36728629531216805No Hit
CATTAA1043820.3664542585694255No Hit
CTGAGG1033140.3627048271717502No Hit
GCGCAG1019870.358046123553103No Hit
CACCTA1007500.3537033832544847No Hit
ACGCTA995780.34958883868699825No Hit
CCGGAA986710.3464046305618189No Hit
GAGAAG972530.34142645291958706No Hit
ACCAGG951210.33394163293845996No Hit
TTAGTA944410.3315543545204644No Hit
CAAGCT935010.3282542931779412No Hit
AATTGC929630.32636553466488No Hit
GACCGA911640.32004977896785947No Hit
AAGGCG907200.3184910265890506No Hit
AGATGC876810.30782199848274416No Hit
TTGAAT871500.3059578148945741No Hit
CAACGG870620.30564887298165705No Hit
TATGGT868210.30480279342469097No Hit
GCATAA863920.30329670159922023No Hit
CCTTAT861930.30259807159160096No Hit
TTACGT834240.29287693344538096No Hit
ACCTGC823210.28900462742325No Hit
TTCGTT808310.28377367912499507No Hit
GGCTAC793840.2786936910796428No Hit
AAGAGG789620.27721217417906324No Hit
TTCTAC789520.27717706714350443No Hit
AGGTCA787480.2764608836181058No Hit
CATGAG762260.26760688925018705No Hit
GCGGCT761790.267441886183061No Hit
TCTCTG753170.26441565971789605No Hit
AGGCTT749950.26328521317290404No Hit
GCCAAT745060.26156847913408077No Hit
ACTTGA734210.25775936577595554No Hit
CCACTT728110.2556178366068713No Hit
TGTGAT724690.2544171759907618No Hit
CAGCCT721800.25340258266311366No Hit
ATTGCT717590.25192457646609No Hit
GCACAT712820.2502499708699373No Hit
CTCGTC709750.24917218487828335No Hit
GTACGC670890.23552959086015013No Hit
TCATGT660450.2318644163478158No Hit
GAGTGA647020.22714954147227462No Hit
CCATGA646780.22706528458693362No Hit
TATTCT643200.22580845271393005No Hit
ACCGCT641030.22504663004230502No Hit
CCTGCT636950.22361426299150772No Hit
GGAGGC623860.21901875203686633No Hit
CGCTTG622070.21839033610036454No Hit
AGCCTA617850.216908819199785No Hit
TTAGGC616840.2165542381406415No Hit
ACAGCC611910.21482346128759477No Hit
CGTCAG607910.21341917986524447No Hit
ATGGCC600020.21064923475965844No Hit
GAGTCG599320.21040348551074717No Hit
TCGTGG593460.20834621322700395No Hit
GTGGTG593150.20823738141677178No Hit
TGACCA583670.20490923444580156No Hit
CAGTAG582480.20449146072265234No Hit
AATCGG576610.20243067773535328No Hit
AACTAG550070.19311327049805893No Hit
GTTACA530830.18635867685655394No Hit
GGTGGT529070.18574079303071983No Hit
AGACGG524400.18410129447012583No Hit
AGTAGG516940.1814823096174425No Hit
CGATGT515150.18085389368094074No Hit
CCGACT514040.18046420558623852No Hit
CACATT510680.17928460919146427No Hit
GAATAC508730.1786000219980685No Hit
AACAAT505310.17739936138195897No Hit
ACGTAA499090.17521570377020423No Hit
TACGGC495230.17386057219763618No Hit
GATTCA489970.17201394212724552No Hit
GTAAGA487830.1712626515662881No Hit
CGAATT471020.16536115888886094No Hit
ATGCCG468190.16436762978254807No Hit
GATCAG466790.16387613128472547No Hit
GAGATA458340.16090958678001044No Hit
CCTCGG455230.15981775797413306No Hit
ACAGTG453240.15911912796651378No Hit
AGTCAA450950.15831517685221824No Hit
GGACTA425000.1492049011247206No Hit
GGATTC423000.14850276041354543No Hit
GCCTGG415360.14582058289685634No Hit
GGCCAG413570.14519216696035456No Hit
CTGGAG386350.1356360318812607No Hit
ATCCAC383700.13470569543895364No Hit
GGCGTT381370.13388770151043458No Hit
TAGCTT348620.12239014736494139No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)