FastQCFastQC Report
Sun 14 May 2023
EGAF00001836166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278727488
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGT23304110.8360894064384493No Hit
CAGATC21267110.7630072711020163No Hit
GCACAT21262330.7628357774314674No Hit
CCTCGG20825940.7471792663664375No Hit
TCGTGG20379230.7311525011842391No Hit
GTGGTG19706970.7070336026563695No Hit
ACAGCC19667480.7056168066208095No Hit
CCGCTC19541550.701098773580595No Hit
TGACCA19141470.6867449686196719No Hit
GCCAAT19046650.6833430795315028No Hit
CTGTTC18818030.6751408027614413No Hit
AAGCGC18727210.6718824230210119No Hit
GAGTCG18664990.6696501351169211No Hit
TTCTGT18565610.6660846453723287No Hit
GGTACC18244480.6545633561624177No Hit
GGCTAC18148390.6511159028563412No Hit
TGTGTG17718560.6356947471216042No Hit
CGTCTC17629100.6324851605594063No Hit
GAAGAT17606210.631663928316966No Hit
AGCCTA17601040.6314784424850124No Hit
GTACTT17537730.6292070482836626No Hit
ATTCAT17518560.6285192797346202No Hit
CCTGCT17331840.6218202633821318No Hit
ATACCT17321170.6214374521970363No Hit
CAGCCT17165140.6158395113150806No Hit
CTCCGC16764080.6014505465639615No Hit
GTAAGA16501970.5920467377799494No Hit
CTTAGC16487030.5915107303661418No Hit
GGCCGT16471250.5909445859893087No Hit
TAGCTT16419850.5891004908708537No Hit
CCTTAT16409980.5887463815553061No Hit
ACGCTA16298700.5847539515011881No Hit
TTGAAT16239750.5826389824888746No Hit
TGCGAC16094430.5774252878854919No Hit
ATCCGG16055910.5760432928667588No Hit
GGCCAG15945180.5720705953479551No Hit
GCTTCT15649050.561446239561417No Hit
CTGAGG15585970.5591830971475623No Hit
CAGGAC15514000.556601005208356No Hit
TCTCTG15383970.5519358750867083No Hit
GTCTGC15273640.5479775285026787No Hit
TTAGCG14925610.5354911389292182No Hit
TGTGAT14843510.5325456095668613No Hit
ACTTGA14818900.5316626683048928No Hit
CGATGT14778890.5302272160541267No Hit
TGATAA14764860.5297238570169297No Hit
AGTTCC14762490.529638827728394No Hit
CACCTA14728630.5284240210997776No Hit
CGTCAG14715640.527957974492993No Hit
TCATGT14664700.5261303829495281No Hit
ACCTGC14664400.5261196197484476No Hit
ACCAGG14610840.5241980295822133No Hit
TTAGTA14507290.5204829313426023No Hit
TCCTCC14487010.5197553389495622No Hit
CGAGAG14477710.5194216797160673No Hit
CGTTGC14338900.514441546576131No Hit
ATGGCC14123490.5067132094269798No Hit
TAAGTC13917330.4993167376444766No Hit
AGGCTT13905850.49890486581646365No Hit
GGCGTT13766990.49392293880968063No Hit
TACGGC13712380.4919636774396646No Hit
ATGCCG13702520.49160992689748634No Hit
ACGTAA13667200.49034273935694495No Hit
GAGATA13585220.4874015152750202No Hit
GCATAA13562570.48658889359344426No Hit
CTCGTC13534320.48557535882503267No Hit
CGAATT13460750.4829358631467306No Hit
GCGCAG13446600.4824281988291015No Hit
CACATT13425870.48168446163444056No Hit
GACTAA13387700.48031502368363466No Hit
AACCTT13363430.4794442807162242No Hit
CCAGCG13346610.4788408239089788No Hit
GCTGTC13324980.47806479711107647No Hit
CTGTAT13298770.4771244521100122No Hit
GTTACA13285650.47665374144942607No Hit
CTATTA13257720.4756516874288337No Hit
CAGTAG13140580.4714490161802772No Hit
GCGTAT12985770.46589484564938205No Hit
CATGAG12931630.46395244662772556No Hit
TTCGTT12876870.4619878036571693No Hit
AGTCAA12866250.4616067863389204No Hit
CTACCG12832670.46040202536464575No Hit
GGCAGG12810490.45960626603142923No Hit
AATGTA12704850.45581618415762426No Hit
CAAGCT12644260.4536423763127374No Hit
CCACTT12629500.45311282681957793No Hit
CATACG12584550.45150014052435333No Hit
ACTAAT12530260.4495523599021565No Hit
AAGCAT12485500.4479464903009494No Hit
GATCAG12308440.4415940490232524No Hit
GCCTGG12304610.4414566388227917No Hit
CTAGTT12263400.43997813376770356No Hit
TATGGT12189110.43731280640680836No Hit
TCCACG12186000.4372012278889408No Hit
AGAGGT12127890.4351163958396525No Hit
GGACTA12109920.43447168009493203No Hit
CCGACT12054680.432489816002647No Hit
GGTGGT12047880.4322458501114896No Hit
CCATGA12039450.4319434041611282No Hit
TAGAGC11942830.42847693586647617No Hit
GCTCAA11928800.4279735768292792No Hit
ACAGTG11882780.42632250178353415No Hit
GTACGC11865150.4256899843333715No Hit
TTAGGC11861880.4255726654415944No Hit
GAGAAG11829790.42442136169935274No Hit
CGTACT11808330.4236514340487293No Hit
AACATA11797820.4232743632375433No Hit
AGACGG11724480.42064311934673626No Hit
GTCTCA11708210.4200593950748051No Hit
GAACCG11648070.41790173203153896No Hit
ACCGCT11567720.41501898800881815No Hit
CGAGCC11556900.4146307952231823No Hit
GACCGA11513670.41307981794748566No Hit
AGTTAG11510240.4129567586817989No Hit
TCCAGA11499300.41256426061573087No Hit
ATTGCT11438760.4103922466376908No Hit
GAGCAC11424200.4098698726119183No Hit
GGATCG11354000.40735128355908695No Hit
AGGTCA11336020.4067062090409971No Hit
GATTCA11158030.40032040183995055No Hit
TTCTAC11149660.40002010852980524No Hit
CTGGAG11112390.39868296018224075No Hit
AGAACG11048300.39638358165808174No Hit
CCTGAC11014100.39515657673490745No Hit
GAGCCA11002910.3947551093346057No Hit
TTCACA10970610.3935962713516078No Hit
GTTGGC10937080.39239330424417995No Hit
CATTAA10902510.39115302470633967No Hit
GGTAAT10901710.39112432283679177No Hit
CAACGG10892950.39081003736524184No Hit
CGCTTG10891510.3907583740000556No Hit
AAGGCG10889810.3906973825272662No Hit
TCAAGC10873470.3901111468417496No Hit
ATTCGA10794210.38726750911628777No Hit
GAGTGA10721990.3846764478428479No Hit
AGATGC10713590.38437507821259453No Hit
GTCGAG10674830.382984472632997No Hit
CTTCAC10659640.38243949588495557No Hit
ATCAGC10609960.3806571097860287No Hit
ATGTGA10371890.3721157921819322No Hit
AGGTGT10327050.37050705239377035No Hit
TCTCGC10200020.3659495542829274No Hit
CTATGC10050450.36058338099757137No Hit
GGAGGC10047830.3604893823748019No Hit
TCAATG9877440.3543762429344608No Hit
TGCGGA9813320.3520757880901937No Hit
GATCCT9801040.35163521439263284No Hit
GAATAC9781850.3509467282968517No Hit
TGACAC9746490.3496781056628329No Hit
TACTCG9654820.34638923018600876No Hit
AACTCT9542530.34236056402158654No Hit
AATCGG9533350.342031210068524No Hit
TAATGA9528440.34185505234417357No Hit
CCGGAA9429240.33829602052022945No Hit
ACACGA9356420.33568343284462854No Hit
AGGACT9333010.334843544386982No Hit
ACGAAC9295860.3335107013198497No Hit
TGGCAT9237290.33140936569557145No Hit
TCTTCA9074040.3255523904409457No Hit
ACTAGC9000280.3229060780686259No Hit
AATTGC8937250.32064472952161793No Hit
GACCAT8554160.30690048051521923No Hit
CGTGAA8304440.2979411919358309No Hit
AACAAT8214940.2947301702801555No Hit
TACAGT8182360.29356128664281583No Hit
TTCCAT8175790.29332557253915337No Hit
TGCATA8172390.2932035895935747No Hit
ATCCAC8147970.29232746502562384No Hit
TATTCT8048190.2887476243462575No Hit
CAAGGA7989100.28662763250677303No Hit
GACACG7817130.2804578068740748No Hit
AGTAGG7410920.26588407383774076No Hit
AAGTTG7368680.2643686151256097No Hit
CATAAC7367070.26431085261314446No Hit
GGATTC7344300.2634939256511364No Hit
GCGGCT7252560.26020253876072674No Hit
AGTGCA7236460.25962491363607454No Hit
CCGTCG7119430.2554261888945808No Hit
AAGAGG7043870.25271529731577824No Hit
ATTGAA7004230.25129311967967793No Hit
TATTGG6990760.25080985195116456No Hit
CGAAGC6902160.24763111989873063No Hit
TATAAG6852640.24585447417371337No Hit
TCGGAC6637250.23812685457130084No Hit
CAGTCC5679540.20376677021535816No Hit
GCCATA5611360.2013206533831353No Hit
AACTAG5329500.1912082671946586No Hit
GACTTG4962180.17802980379172362No Hit
TACGAA4412790.15831915365304766No Hit
CGACAA4315160.15481644924809138No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)