FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001836168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29037377
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGC5430221.8700793807925558No Hit
ACCAGG5107631.7589846355612628No Hit
AAGCGC5052991.7401675089316777No Hit
AACATA4995321.720306899621133No Hit
CCGCTC4985161.7168079609945486No Hit
CGTCTC4799361.6528214652446052No Hit
TCCTCC4733401.6301059148696524No Hit
CATGAG4689451.6149702502398893No Hit
GACTAA4618161.5904191346208716No Hit
CTCCGC4297841.480106140440991No Hit
GTGGTG4276071.4726089067893426No Hit
TCATGT4214091.4512640036322841No Hit
TGCGAC4120761.419122670756384No Hit
GCTGTC4100231.4120524729213662No Hit
TCCAGA4028891.3874841381161942No Hit
CTGAGG3950091.360346700736778No Hit
GGCCGT3785061.303513054915394No Hit
TGTGAT3749291.2911944491404992No Hit
CCAGCG3673251.2650075108368086No Hit
TTCTAC3621481.2471787654924893No Hit
TTCACA3615081.2449747096647195No Hit
GCTCAA3603361.2409385324301159No Hit
GCGTAT3565081.2277555235102675No Hit
GATTCA3561821.2266328325729972No Hit
ATTCGA3480461.198613772862473No Hit
CCACTT3473701.1962857388943913No Hit
CTACCG3458361.1910028925822054No Hit
AGAGGT3421791.1784087798288392No Hit
GCGCAG3414111.1757639128355155No Hit
AGAACG3332361.1476105434729866No Hit
CGTACT3327671.145995383811699No Hit
GTTGGC3324121.1447728215947328No Hit
AGGTCA3180431.095288324424069No Hit
GGTAAT3179221.0948716201191313No Hit
GACACG3157621.0874329317004081No Hit
TCTTCA3154111.0862241448323655No Hit
TGCGGA3143001.0823980416688463No Hit
ATTGCT3139851.081313232941116No Hit
GTCGAG3119881.0744358899910278No Hit
TACTCG3111011.0713812063672281No Hit
ATGTGA3072611.0581568714006089No Hit
CCGGAA2864390.9864492925790095No Hit
TCTCGC2845410.9799128895147795No Hit
AATTGC2843230.9791621329984453No Hit
AGGTGT2752740.9479988498961183No Hit
GCCTGG2747680.9462562682572879No Hit
CTGTTC2722100.9374469326206702No Hit
ATCCGG2629490.9055535560253944No Hit
CGCTTG2506680.8632597909928297No Hit
GTAAGA2417500.8325476505677493No Hit
TAAGTC2252950.775879308933448No Hit
AACCTT2164290.745346248044374No Hit
GAGATA1954520.6731048744519865No Hit
AAGCAT1926540.6634690178799552No Hit
GCGGCT1913800.6590815692478009No Hit
CACATT1901190.6547388904996481No Hit
CATACG1888240.650279121285645No Hit
TCAAGC1853200.6382119156286051No Hit
AGTCAA1848650.63664496968855No Hit
CGAATT1800070.6199148084208845No Hit
GGACTA1691170.5824114209764883No Hit
TAATGA1688250.5814058205050684No Hit
ACGAAC1655270.5700480453175919No Hit
CTATGC1505220.5183732676680817No Hit
TTACGT1497410.5156836307907564No Hit
TGCATA1412370.48639723897926457No Hit
CTGGAG1377290.4743162579733011No Hit
AATCGG1258930.43355500050848256No Hit
GTACTT1255610.432411646547827No Hit
ACCGCT1230670.42382271649398634No Hit
GCTTCT1218330.4195730213510676No Hit
TGTGTG1160180.39954710785344005No Hit
ATCCAC1154540.3976047836552179No Hit
TATTCT1128650.3886886890644427No Hit
TGGCAT1125340.387548778941018No Hit
AATGTA1116170.3843907802002915No Hit
TTGAAT1100250.378908191328714No Hit
AGTTCC1097320.3778991470200631No Hit
TTAGCG1089230.3751130827002728No Hit
CAGCCT1068630.36801877800463867No Hit
CGTTGC1066090.3671440433479925No Hit
GAGCAC1054350.3631009784389272No Hit
ACGCTA1024410.3527901297696414No Hit
CATTAA1004590.34596444437801666No Hit
GTCTCA961390.3310870675405702No Hit
GGATTC958710.33016411916269156No Hit
CGTCAG949510.3269957889102725No Hit
GACCGA946310.32589376099638756No Hit
TCAATG946090.3258179965773079No Hit
TAGAGC939810.3236552667963088No Hit
GGCAGG939760.3236380476101543No Hit
TCTCTG924160.3182656615299653No Hit
GAAGAT900090.3099763453152122No Hit
TTCGTT897910.30922558879887807No Hit
ACTAGC849690.29261940567152467No Hit
CTTCAC847810.29197196427211725No Hit
AACTCT840240.2893649794883333No Hit
GGTACC836670.2881355295969054No Hit
GAGAAG822810.2833623711948913No Hit
CTCGTC789060.2717394205406363No Hit
AGGACT784900.2703067842525859No Hit
CCTGAC782720.2695560277362518No Hit
CAGGAC769690.2650687078244016No Hit
TGACAC735340.2532391269362932No Hit
CGAGAG724460.24949223202908444No Hit
AAGGCG715370.2463617839862051No Hit
TACAGT711430.24500491211723427No Hit
CTATTA711340.24497391758215628No Hit
CAAGGA709040.24418183501905147No Hit
AGCCTA707840.24376857455134462No Hit
GTACGC698350.24050037301922966No Hit
TCCACG654300.22533027001715752No Hit
CCTTAT649160.2235601376804799No Hit
AAGTTG640370.22053300475452725No Hit
GGCCAG613800.2113827292320515No Hit
ATGGCC587210.202225566035114No Hit
CGAGCC585390.20159878765909195No Hit
GAGCCA574470.19783811740295962No Hit
CGTGAA568720.19585791099519767No Hit
GGATCG561220.19327503307202987No Hit
ATGCCG555670.19136370340888573No Hit
CAGTAG532710.18345665312676143No Hit
GCACAT524700.18069813950481822No Hit
ACACGA517430.17819446983796092No Hit
GGAGGC507100.17463698597845115No Hit
ATTCAT499130.17189224770543152No Hit
GATCCT484940.16700544267479808No Hit
CCGACT480540.16549015429320632No Hit
GAATAC444030.15291670456322554No Hit
ATACCT440510.1517044738579521No Hit
TTCTGT433280.14921457954001838No Hit
TCGTGG427490.14722059778333285No Hit
AGACGG419180.14435876904446293No Hit
GGCGTT418470.14411425660106972No Hit
TGATAA407400.14030192878647407No Hit
ACAGCC387320.13338670362684618No Hit
CACCTA386820.13321451176530166No Hit
CCTGCT383530.13208148931633873No Hit
TTAGTA382730.1318059823378675No Hit
GGTGGT375680.12937807709008978No Hit
TATTGG364100.12539011357671873No Hit
GTCTGC357420.12308963030648395No Hit
AACAAT352930.12154334738981418No Hit
ACCTGC346370.11928419016635008No Hit
CCATGA337050.11607453386716024No Hit
CAGATC335320.11547875002621621No Hit
TGACCA333200.11474865653326743No Hit
ACTAAT328510.11313349687197988No Hit
CAAGCT320760.11046452301803981No Hit
GCCAAT312300.10755103672070655No Hit
GAGTCG311170.10716188311361594No Hit
ATCAGC310300.10686226927452847No Hit
CCTCGG298160.10268145087622756No Hit
ACTTGA293180.10096641993524415No Hit
AGGCTT290640.10009168527859799No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)