FastQCFastQC Report
Thu 23 Mar 2023
EGAF00001836170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412234598
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGTAC42903281.0407491318814535No Hit
CAGCTAAC42462791.0300637114403484No Hit
TTGACCGA37334810.9056690093731531No Hit
TCCGGTCT36719110.8907333391749909No Hit
ATGCGGCA35399950.8587331139052041No Hit
AGCCACGC34692620.8415746802503947No Hit
CATACGTT34684710.8413827992186139No Hit
TTATCGTC34584800.8389591792584086No Hit
GGTCGTAT34201930.8296715066113883No Hit
TGAGCTCC32255790.782461980544389No Hit
GAGTCCTA30728200.7454056537001293No Hit
TTAGCAGT30543230.7409186455524046No Hit
TGCAATCG29747430.7216141038215331No Hit
GTTCTAAT29132840.706705360038703No Hit
GATCTTCC29100480.7059203701286616No Hit
ACGCTATT28511090.6916229287479649No Hit
ATGGCGCT27873800.6761635276425779No Hit
TGGAAGAT27497370.6670320767205474No Hit
CCATTGAC26798270.6500732866677047No Hit
GCTGCATG25925200.6288943268172751No Hit
TAACTATG25817590.6262839200119733No Hit
CAGGAATC25785560.6255069352524361No Hit
GCCTACAT25765400.6250178933307291No Hit
GATCGATT25681220.6229758522112208RNA PCR Primer, Index 42 (100% over 8bp)
ACGGACGG25673980.6228002240607665No Hit
ACACTGTG25538300.6195088943019771No Hit
GTCTCCAC25281650.6132830704326278No Hit
GTGTTAAG25131030.6096293256782878No Hit
TACATGTC25030500.6071906657383473No Hit
GTGGTCGT25005150.6065757246314392No Hit
GGCGTGTC24998340.606410527434672No Hit
CAACAGCA24912130.6043192425105474No Hit
CCTGGCCA24819130.6020632455502922ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GTAGAATG24817780.6020304972073207No Hit
GCACTCTC24752530.6004476606303676No Hit
ATTGCCAG24658390.5981640095138254No Hit
GATCGGCG24646950.5978864976296823No Hit
CTACAAGC24507010.5944918286552939No Hit
AAGCTCCA24488990.5940546989217047No Hit
CGCCGGTA24474430.5937015019782498No Hit
TCCTCCTA24114960.5849814672760679No Hit
GCCATCGC24066510.5838061656338704No Hit
ACTGAGGC24024340.5827832044315698No Hit
CGTTCATA23907410.5799467127696061No Hit
TCCGGCGA23683600.574517522665577No Hit
ATATTCGG23672590.5742504417351209No Hit
TATACTAT23449320.5688343509682805No Hit
TGTATATT23412630.567944323780412No Hit
CTCGTATC23380320.5671605467719621No Hit
CTGTCATT23033450.558746163270847No Hit
AATGCGAA23012220.5582311652550813No Hit
TCAATAAC22978370.557410030877612No Hit
GAAGGTGA22718060.5510954226117625No Hit
ATGTCAAC22679950.5501709490186945No Hit
GCAGTTAA22595490.5481221156502735No Hit
CAATCGTA22582880.5478162218688883No Hit
TGGCGCGA22477200.5452526330650199No Hit
GTCATACA22396240.5432887028080065No Hit
CCGAATAG22194480.5383944023058443No Hit
TAGCCACC22136660.5369918029053933No Hit
GCAGTGGT21845760.5299351414458424Clontech Universal Primer Mix Long (100% over 8bp)
CTTCTAGA21380530.5186495772972457No Hit
CGACTCTG21244180.5153419946571296No Hit
AGGTAGCC21135090.5126956859647185No Hit
CTCTCGAA20756950.5035227538082575No Hit
AACAGTGA20689870.5018955250330541No Hit
CTAGTCCG20430100.4955940161043931No Hit
TCGTCGAC20403770.4949553021262907No Hit
TGTCGGCT20385420.4945101672421974No Hit
TGCTAACT20172500.4893451471048046No Hit
CACATTAT20153530.4888849722409762No Hit
CCACATAT20152880.4888692045202863No Hit
TTGTGTAC20116890.4879961579546994No Hit
AGTCATGA19732300.4786667614929303No Hit
CATTGTGA19666720.477075919765473No Hit
TACGTACC19657230.4768457110433996No Hit
GATACGAG19534150.473860032485677No Hit
CGACCTCG19486190.4726966172790766No Hit
AATGGTCT19401290.4706371103766501No Hit
ACCGAACT19214260.46610013068335426No Hit
TGATTCGC19210320.4660045540379413No Hit
TACGAGCG19186190.46541920772986645No Hit
TCCGCGAT19149700.4645340321483642No Hit
CCGCTGTA19110890.4635925779330147No Hit
TTGCGTCG19091650.46312585340059204No Hit
AAGGCCTG18824670.45664944406243163No Hit
GCAGATCT18668110.45285160659901724No Hit
GCTTCTCA18623220.45176266355013706No Hit
ATACCGAA18527590.44944286796616717No Hit
GCGGAGTA18494740.4486459916205287No Hit
CGTGTTAG18359200.4453580579861955No Hit
TGCTCAGC18272770.44326143629506803No Hit
CAGATAGT18248530.4426734216034919No Hit
AGTCTACC18208710.44170746677599343No Hit
CACAGGCA18049440.43784389004631774No Hit
GTCCGACT17916680.43462339374047393No Hit
TGCTTCTT17798100.4317468763259895No Hit
AGAAGTAG17677640.4288247538116633No Hit
GGAACCTG17506200.424665956834608No Hit
GCGACAAG17415570.42246745140979164No Hit
CGTATGAC17224140.4178237363764407No Hit
CAGAGTGC17209320.41746423234470975No Hit
GTAATTCC17146950.4159512588994289No Hit
AGGATCTT16925770.4105858674191146No Hit
TCTCGAGT16897230.4098935431906664No Hit
CGAATCGT16835300.40839124327939114No Hit
TCGTGTCA16800270.4075414844243617No Hit
TTAGGATC16788100.407246264176982No Hit
TAAGACTG16784000.40714680624647615No Hit
ACATACTC16688270.4048245848593232No Hit
TTACCTAC16536380.40114003240455814No Hit
GCGCCTAA16495850.40015685437445986No Hit
GACGATGT16476440.39968600597662596No Hit
CTCCAATG16461800.3993308683906245No Hit
GCCTGGTC16426490.3984743172866825No Hit
GGATATGC16421420.3983513290653008No Hit
AACACGTA16339430.3963624130355016No Hit
GGCAACAC16210290.39322973080488505No Hit
CTAGCGGC16160860.39203065629149353No Hit
AGAACACA16113610.39088446428749296No Hit
TGCTCGAG16102240.39060865046557786No Hit
TCATTGCG16024060.38871215753705374No Hit
CTGTAAGG15902370.3857601976435758No Hit
ATCTCAGT15810200.3835243348497401No Hit
GTAATCTT15742380.38187915513098203No Hit
CGTACACT15693430.3806917244728692No Hit
GCTGTAGC15674700.3802373715366802No Hit
GCGCAATC15654690.3797519683197479No Hit
ATGAATTG15562430.3775139223030475No Hit
TAGCATGG15444980.3746648164645317No Hit
CGTTGTCT15305480.371280821024149No Hit
GTGAGTCA15265340.3703071036264647No Hit
AGCTCACG15125440.3669134049733497No Hit
TACAGCCT15050590.36509769129082176No Hit
AATTCATT14979690.3633777968340251No Hit
TGTTGCGT14967700.363086943032375No Hit
AAGCCTGT14877720.3609042053282485No Hit
GTTAATGG14611520.3544467172549161No Hit
AGATGACT14527430.3524068593582725No Hit
CTCGAACA14492980.35157117016170486No Hit
AGGCGGTT14430340.35005164704782976No Hit
TCGTTCTG14369320.34857142194552043No Hit
ATACTTAG14278990.34638019393025327No Hit
CTCTGAGA13969020.33886093180369103No Hit
GTTAAGCC13876570.3366182767609428No Hit
TAGGTCTC13852490.3360341433544595No Hit
CAATACAT13609930.3301501151536049No Hit
CTTGCTTG13595020.3297884278990091No Hit
GTATCTAG13542710.32851949025394517No Hit
AGACCATC13520760.3279870264552613No Hit
CACACATG13486730.3271615256320626ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
GGTTCAAT13073190.3171298591487947No Hit
GAGACTGA13025540.3159739639320618TruSeq Adapter, Index 15 (100% over 8bp)
GAACAGAC13007020.31552470518255726No Hit
GTCGAAGC12990820.3151317250668999No Hit
GCCGGTTG12907530.31311127359572083No Hit
TTGTGGCT12891850.3127309076566155No Hit
TAGCGCTG12716030.3084658605001417No Hit
GTCCATCC12589360.3053930956081469No Hit
CGGACATC12385440.3004463977572305No Hit
AATTATTG12351880.2996322982089922No Hit
GCATAACG12192520.2957665382564517No Hit
TCCGTATG11901340.2887030845479884No Hit
GAAGGCAG11878110.2881395704685612No Hit
TATAGGTG11856090.28760540860764916RNA PCR Primer, Index 39 (100% over 8bp)
CAGTACCG11352620.27539221732184643No Hit
AGAAGAGC11303460.2741996924770492No Hit
AATATCAA11256520.27306102046291614No Hit
CAAGAGAG11244730.2727750182676322No Hit
TTATGACG11207630.27187504528671314No Hit
GAACGCGC11162820.2707880428803795No Hit
CAATCAGC11111750.2695491851947856No Hit
ACGCATTA11023480.26740792872508967No Hit
ACCATAAG10941600.26542168107879194No Hit
ACTTGTCC10720670.2600623541064353No Hit
CCGGCTGA10663280.25867018565967137No Hit
TGAGTAAG10655440.25848000269011867No Hit
GAGCTTAG10368830.25152740818712166No Hit
AACTTGAA10032640.24337210046595845No Hit
AGCGTGCG9935890.24102513588633823No Hit
AAGTGGAG9543840.23151477450711208No Hit
GACTAAGA9370860.22731862016103752No Hit
AGAACGGT8917090.21631105305722056No Hit
CGGAGCTG8668370.21027759538028876No Hit
CGATGGCA8626770.20926846125613163No Hit
GACAGTCG8584700.20824792585701404No Hit
AACGTCGA7517870.18236872975906793No Hit
CATTCCAG7058380.17122240671317937No Hit
GTAGCACA6860630.16642538091865838No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)