FastQCFastQC Report
Thu 23 Mar 2023
EGAF00001836174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413440070
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGCCGC45826811.1084269117891743No Hit
CCAAGACG45504011.1006192505723984No Hit
ACAAGTGT43717441.0574069417122534No Hit
CCTTGGTT42930691.0383775815440432No Hit
ACTGGTTA39362950.9520835752567476No Hit
CCTGGTGC38930630.9416269206804266No Hit
CTGATTAC38927460.9415502469317983No Hit
CTATCGCT38319080.9268351758938121No Hit
GAGCGTTA37921970.9172301562352193No Hit
TTAGGTCA37764560.913422832963433No Hit
CGATCTAA37674370.9112413801594025No Hit
GTCCGCGG35918770.8687781520547827No Hit
CCAGGATA35811290.8661785007921462No Hit
ACTGTCAT35200290.8514000590218553No Hit
GTGTAGAT34069380.8240463968574696Illumina Single End PCR Primer 1 (100% over 8bp)
TTAAGTGG33818720.8179836076362893No Hit
CTATTGGA33790690.8173056375498389No Hit
CATCAGTG33522330.810814733076066No Hit
TGCTATAC33420080.8083415814050147No Hit
CGGTCGGA32664370.7900629951034983No Hit
GGCTACGG31929690.7722930677715878No Hit
GTCACAAT31429410.76019264412373No Hit
CGGTTGTT31112250.7525213992925263No Hit
TTGATGAG30372890.7346382753853539No Hit
TCATCATG29433110.7119075323298972No Hit
CACGATAC29377280.7105571552365497No Hit
ACCTATAA29317410.7091090614414806No Hit
TCCAGATC28883300.6986091115938521No Hit
CCATAAGA28878990.6985048643204806No Hit
AGCGTTAT28073790.679029248422873No Hit
CAACTCGA27618430.6680153183991092No Hit
ACGATAGA27030620.6537977801716219No Hit
AGAGATCG26773710.647583820310402No Hit
GTGTGTGT26734290.646630356849543No Hit
TCTCTCAG26613240.643702483893252ABI Solid3 Adapter A (100% over 8bp)
AGTCTGAT26269080.6353781818970764No Hit
TACCTGCA25564830.6183442741773917No Hit
CACGCCGT25294320.611801367003445No Hit
CTGCCTTA25023680.6052553154801856No Hit
AACGCGCC24667450.596639072743965No Hit
CGACGCCT24656310.5963696261951581No Hit
TCAACTTA24516320.5929836457312906No Hit
AGCAGGAA24432020.5909446561384338Illumina Paired End Adapter 2 (100% over 8bp)
CCTATACC23992970.5803252210169179No Hit
CTCATAGG23506700.5685636614757733No Hit
AATCAGCT23494150.5682601108305733No Hit
CCGTTACT23436430.5668640197356778No Hit
TGGTTCCA23250470.5623661489801896No Hit
CAGCACAA23103500.5588113411455257No Hit
AGACTCAA23026720.5569542400667646No Hit
CATGACTA22875950.5533075204829566No Hit
TCTCATTC22869920.5531616710494461No Hit
GCGTCGCT22772850.55081380960486No Hit
GCTAGTCT22719510.5495236588945044No Hit
ATCTAACC22708120.5492481655200958No Hit
CGAGTTCT22191280.5367472001443885No Hit
GCGCTCAT22144840.5356239418206368No Hit
TTCGACAA22057500.5335114228284645No Hit
ATTACGAC22016630.5325228877791163No Hit
CTCGGCCT21826360.5279207697502566No Hit
TTGACTCT21709570.5250959347022169No Hit
TCTCTGCC21601640.5224853991534977No Hit
CAAGGTCC21288930.5149217878180022No Hit
TTGCTCTA21264600.5143333107504553No Hit
CCTGTGCG21123650.5109241104762777No Hit
CATGTATT21056520.5093004168657382No Hit
ACAGTGCC21055250.5092696989916822No Hit
AAGAACGT20724330.5012656368793669No Hit
TGGTTAGT20662100.49976046105061855No Hit
GCACACCA20646880.4993923303079936No Hit
TGCGCCTG20598930.4982325491576083No Hit
ACCAGGCC20499770.4958341362509928No Hit
TGAGATAA20412120.4937141191950746No Hit
TGGCACAG20304660.49111495167848634No Hit
CGGTATAT20256870.489959040496486No Hit
CGTAGTTC20116460.48656290136560787No Hit
GCGGCTCC20072290.4854945482183186No Hit
GCTGACAA19959260.48276065742732677No Hit
GACATCTA19917370.481747451329524No Hit
GATGGTAC19769050.4781599906366115No Hit
TAGGTGAA19354280.4681278232175222No Hit
CGCGGTCG19298870.4667876047911853No Hit
AGTGCTCA19277610.46627338274202595No Hit
AACGGCCG19264470.4659555615884063No Hit
AGTGGCAA19203570.46448255487185847No Hit
GTATATCA19201110.46442305410793877No Hit
CGACTTAC19115690.4623569747363868No Hit
GACAAGGC18936500.4580228520181897No Hit
AACAATAG18828330.4554065115168929No Hit
AATCCAAG18687100.4519905387980415No Hit
CATATCTC18397330.44498178417974826No Hit
CTGTGTTG18384270.444665898010321No Hit
ATGTAATA18380840.44458293556306727No Hit
AATGTAGG18247770.4413643312318518No Hit
CCACCAGG17627410.42635949631103726No Hit
TATGAGGT17441970.4218742029528004No Hit
GGACGTTG17413780.4211923628979649No Hit
GAGTTCCT17375680.42027082667628224RNA PCR Primer, Index 1 (100% over 8bp)
TTCAGTAA17364450.41999920327025875No Hit
CCTTGTAG17205590.41615680840998315No Hit
GCATCGAA16788870.40607747575120134No Hit
TTCCGAGC16684100.40354337207808616No Hit
AGCAACTA16675840.4033435849602096No Hit
TTCTATGA16664390.4030666403476567No Hit
GACGCAAG16531120.39984319855596No Hit
AGGTCTTG16425470.39728781005672725No Hit
TGAATTGA16271600.39356610983545937No Hit
AACAGAAT16210310.392083670070973No Hit
CTCAATTC16106230.38956625563651825No Hit
GATTGAGG16081010.3889562518698297No Hit
ACACGAAT16006700.3871588934280124No Hit
CTTCGACG15917720.38500670725989383No Hit
CTGCGCTT15866880.38377702480555403No Hit
TGACGACC15866800.38377508982136155No Hit
TATGCTTA15805950.382303292469934No Hit
CATTCTTC15741750.3807504676554452No Hit
GTGATGTC15597300.3772566118228453No Hit
AGGACCAG15466410.3740907358108758No Hit
CACACCAC15361150.3715447803595815No Hit
GTCGACCG15339010.3710092734843045No Hit
TGCCGTAG15260760.3691166170710062No Hit
ATGGAGAG15236670.3685339449560368No Hit
TGTAGCAC15050540.3640319623591395No Hit
GTGCCACG15037860.36372526736462674No Hit
CACTTGCT15025060.36341566989382523No Hit
CTTCTGCT14894200.3602505195009279Illumina Single End Adapter 1 (100% over 8bp)
TATCACAT14440990.3492885921773378No Hit
AGAATGTA14398690.3482654692855484No Hit
TAAGTCCT14267460.34509136959076075No Hit
TTCCTTAT14222290.3439988291410651No Hit
AATATTCG14107390.3412197080945734No Hit
CTTCGCAA14099950.34103975456467006No Hit
AGTAACCG14058720.3400425120864555No Hit
TCTGATAT13985830.3382794996140553No Hit
TCCACAGG13607170.32912073568485994No Hit
ACCGACAC13494890.326404985370673No Hit
ACGATTCT13263350.3208046573715025No Hit
ATTCCACA13100700.3168705926351067No Hit
ACAACCTT12940070.3129853862495718No Hit
TACAAGAA12911200.31228709882909994No Hit
ACGCCTCG12780000.30911372475338444No Hit
TGTGTCGA12767520.308811867219353No Hit
ATTAGTGC12608400.30496318366045166No Hit
GTAGTGAC12380930.2994612979820751RNA PCR Primer, Index 43 (100% over 8bp)
GCCACTGT12262930.2966071962981237No Hit
AACCGTTC12180170.2946054551509727No Hit
GCACGTCC12084260.2922856509771779No Hit
ACTATTAC11939280.2887789758743027No Hit
ATGAGACG11846300.2865300404965585No Hit
CACCACGG11712600.28329619816482715No Hit
GAGAATTC11651960.281829480146905Illumina Small RNA Adapter 2 (100% over 8bp)
AAGATGGC11607050.280743228395835No Hit
ATACGTTA11495250.27803908798680305No Hit
ACGACGAT11455230.27707111214450014No Hit
GGTTAATG11254920.27222615359948055No Hit
TACCTCGC10965290.26522078520352416No Hit
GGTCTCGC10910280.26389024169814984No Hit
CCTCCATC10766060.2604019489451035No Hit
AAGGTGTT10584610.256013162923468No Hit
CACCGGAG10403210.2516255862669528No Hit
TATACGGA10339120.25007542205572864No Hit
TCAATCGG10180610.24624149275129523No Hit
GCTTATAC10119530.2447641323203143No Hit
CTAGGCAC10110650.24454934907494574No Hit
GCCAACTG10019520.24234516020665342No Hit
TCTGTGTT9903750.23954499620706818No Hit
GTTCACTG9837530.23794331304171848No Hit
CCATGCGG9570280.23147925647361658No Hit
ATACATGT9311220.2252132939122229No Hit
ATTAGGCT9211360.22279794989392296No Hit
CCTAACCT9107810.22029335472974354No Hit
ACTTAAGG8995550.21757808816160468No Hit
GAGAAGCT8849910.2140554494391412No Hit
GCGATCCA8805110.2129718582913359No Hit
CGCCAGCC8766680.21204234025985919No Hit
TTGCAGAA8515350.20596334554606668No Hit
CGTATCCA8450850.20440326454085594No Hit
TCGGAGCT8344400.20182852619969804No Hit
TCTTAGTA8272840.20009768283949836No Hit
TATTGACC8218760.198789633525362No Hit
ATAGCCTA8194700.19820768702946476No Hit
TGCGGATT8186830.19801733295952664No Hit
TTATACGT8165100.19749174287823626No Hit
GTAAGAGA7773340.1880161252875175No Hit
AAGCATCC7761000.1877176539758229No Hit
CTCTAGTT7409900.17922549210094707No Hit
TGGCCGCA7332990.1773652466728733No Hit
GCAAGCAC7163210.1732587264703201No Hit
GCTACCTC6526140.1578497217262952No Hit
CGGCTTGA5486450.13270242528741832No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)