FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406528229
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGTT79857801.9643851103879921No Hit
ATTGACCT51453971.2656924250148442No Hit
CCGGTAGG48157751.1846102328111636No Hit
GCTGGAAT42310631.0407796305825543No Hit
TCACCGAG41311101.0161926541145558No Hit
ACTACATA40671981.0004712366481197No Hit
TAATAGGC36605830.900449892250902No Hit
ATTAATCA36382860.894965156281927No Hit
GCGAGATA35484980.8728786211793426No Hit
CGTGTGGT35367770.8699954265660602No Hit
TACTCCGG35090900.8631848294107024No Hit
TGATCCAT34853100.857335297126439No Hit
ACCTCGCA34391410.8459783982184421No Hit
CGCCTCAT34096250.8387178938070743No Hit
TCTGACCG33060960.8132512736280363No Hit
GTTGCAAC31748260.780960773083239No Hit
AATTAACA31216500.7678802546329445No Hit
TCTAGTGA31167860.7666837817552886No Hit
AGGTTATC30183100.7424601256903122No Hit
GGACACAT30169760.7421319811963168No Hit
TGGCTGTG30098160.7403707258912148No Hit
GACTCGGT29641110.7291279641985207No Hit
TGCAGTGT29097150.7157473435873011No Hit
TACTGTTG28911010.7111685717647913No Hit
ATAGGTAT28865900.7100589317255014No Hit
GACGCCTC28693700.7058230635196554No Hit
GGCGTTGA28134300.6920626414851009No Hit
TCGGATTG27412910.6743175023154419No Hit
GGAATATC26906620.6618635086224234RNA PCR Primer, Index 37 (100% over 8bp)
CAGGCGCA26714300.6571327178364286No Hit
TGTCCTGC26549390.6530761729710042No Hit
GGCACTCC26424470.6500033236314323No Hit
GTTGCCTT26209320.6447109482279028No Hit
ACCTAGGT26092980.6418491543424897No Hit
GGTTGGCC25295570.6222340343307378No Hit
GGTCCGTG24936270.6133957797061124No Hit
AAGCAGGA24539440.6036343419585752No Hit
TAACCTCT24481050.602198033337557No Hit
GCACCACT24164750.5944175158375041No Hit
TTACAGCG24141320.593841172097301No Hit
AAGTGAGA24124970.5934389860045857No Hit
TTCACGGT23519970.5785568706472287No Hit
AGCGAAGA23478770.5775434108906616No Hit
GCGTACGA23410780.5758709563069482No Hit
ATGTGCAT23392400.5754188351825378No Hit
CGATGCTC23350450.5743869265226351No Hit
TCCGACTT23075570.5676252804574612No Hit
CGGCACTC22875150.5626952415154423No Hit
TGAGCGTA22756260.5597707213586882No Hit
GATATTGT22723850.558973482749214No Hit
GGCTGAGT22654810.5572751997008306No Hit
TCACGCCG22644590.5570238026447113No Hit
TTAGAGAT22544310.5545570612760572No Hit
TCTATGAA22519600.5539492314074946No Hit
AACGGATA22414740.5513698287358048No Hit
CCGAAGGT22391880.5508075061621366No Hit
GCATGTAT22377530.5504545171449828No Hit
ATTCTGGC22362900.5500946405372503No Hit
CTTATGTA22101390.543661876922205No Hit
ATAATATG21984630.5407897516509241No Hit
TAGGATCA21975950.5405762363429872No Hit
TAATACCA21956350.5400941050024843No Hit
AACCAACG21811150.5365223973167187No Hit
ACTCTTGT21766880.5354334200491646No Hit
TACTGCAC21763340.5353463412254208No Hit
ATAGTTGC21722860.5343505924160558No Hit
TTAATACT21653760.5326508334554056No Hit
TACTCAAT21562300.5304010511899777No Hit
CGCGTCGG21260650.522980902268413No Hit
ACACTAGC21209680.5217271147977278No Hit
GCCTTAGG21147580.5201995456015429No Hit
TTACAATT21125000.5196441106184535No Hit
CTGGATGC21034590.5174201568176954No Hit
TTCTTGCC21009030.5167914181920193No Hit
CTATCCTG21000000.5165692933958591No Hit
ATCGCTAA20610630.5069913607401664No Hit
AGTGGATG20534600.5051211339126956No Hit
ATCGGCGC20522990.504835544889061No Hit
GATTACGC20500940.5042931471309954No Hit
TTATGCTA20383390.5014015890148676No Hit
TTCGCATA20353190.5006587131738889No Hit
AGTTACAT20240810.4978943294980876No Hit
CGTAATGT20227610.4975696287993816No Hit
GGACCGGC19959620.490977466659517No Hit
TGTCTTAA19764110.4861682065380016No Hit
GCCATGCG19673530.48394007098582076No Hit
TGGAGTTA19586120.48178991279840494No Hit
GATGGCGT19345350.47586732285693256No Hit
ACAGGCTG19331240.4755202374888461No Hit
GTTCAGGA19307650.474939957982598No Hit
GTAGCTTC19007440.46755523095543755No Hit
AATCGCGG18861130.4639562188927353No Hit
TAGATCCG18822130.4629968759192858No Hit
CACTGCTT18792350.46226433146417495Clontech Universal Primer Mix Long (100% over 8bp)
TAATTAGA18740110.4609793038505083No Hit
TAGGCATT18654360.4588699792358085No Hit
ATCATCTC18636220.4584237617604656Illumina PCR Primer Index 7 (100% over 8bp)
CTGACTGG18531500.4558478028840649No Hit
GTACTATA18313520.4504858136186159No Hit
CCATCTCC18294250.4500117997955807No Hit
GCATCCGT18195520.44758318616048676No Hit
TTAACCAG18169100.44693329279231925No Hit
ATAACTGA18152690.44652963078733704No Hit
CCACTACA18060140.44425303611572814No Hit
AGATGGAC18018720.44323416468084925RNA PCR Primer, Index 16 (100% over 8bp)
GCAGCTGG17989330.44251121365547286No Hit
GAGTGAAC17952400.44160278965522953No Hit
GGAGTGCA17943450.44138263274209183No Hit
CGAGGAGT17821680.4383872687965293No Hit
GGCTCTTA17788100.4375612498978515No Hit
TGTGGAGC17654200.43426750568900835No Hit
GCGCGAGG17336690.4264572239582408No Hit
GGTGTACG17222570.42365003882670105No Hit
ATTCGATC17054900.41952560199700173No Hit
CGCTTACC16720380.4112968991385835No Hit
GGATTGTG16392420.40322956268800714No Hit
GTGTCGGC16108400.39624308598751695No Hit
GTGGTACC15710200.38644794824322026No Hit
AGCAAGCT14867800.3657261400167121No Hit
CCAGCGTG14826930.3647207977776126No Hit
GACCTGTG14817900.36449867298145244No Hit
AGTGTCTC14674670.3609754244151149No Hit
TCCTTACA14549320.3578919977042972No Hit
CAGGCTAG14399510.35420689075936224No Hit
ATGGTCAA14396900.3541426885757545No Hit
GTCTTGTA14381770.3537705126991317No Hit
TCGATTGC14175570.34869829420873993No Hit
CAGTGGTC14085750.3464888535452725No Hit
TGACGGAA14060090.34585765506581834No Hit
AGCTTCAC14038740.3453324762841992No Hit
ACTCCGTT13939860.3429001728684381No Hit
GTGCGGTG13716970.3374174047824856No Hit
CTTAACAC13665800.3361586976042444No Hit
AGAGCCGC13613460.3348712101367996No Hit
CTGAACTA13605600.33467786562984286No Hit
ACTCATAG13508070.33227877024008584No Hit
CGAGCCTT13354060.3284903494364717No Hit
TACACACA13257530.32611585258449544No Hit
CCGACACA13197730.32464486002520626No Hit
GTTATCCG13171530.3240003783353505No Hit
GGTAATTA13126640.3228961499743724No Hit
TCGCAACA13029920.3205169794002177No Hit
TCGGCAGC12966010.3189448868506497No Hit
TCGTGAAG12963710.3188883102137539No Hit
TTAAGGAC12679360.3118937159957962No Hit
TAACGGTC12590500.3097078899285983No Hit
CGGAGAGA12572770.3092717578537455No Hit
CTTGCCGA12506510.3076418587403927No Hit
GGCCGTCA12008750.295397690574644No Hit
TCCAACCA11862340.29179621865816363No Hit
CCTACTCG11738380.2887469839148612No Hit
CATCTCCG11661800.28686322789161095No Hit
GAGGTATA11349820.27918897607477094No Hit
GAACAAGT11286950.2776424660044948No Hit
TCTCCACG11265280.2771094156907859No Hit
GGTAGCGA11135570.27391873935524413No Hit
GTTACCAA11078890.2725244942338309No Hit
GCGGAAGT11028270.2712793162513691No Hit
CGAGAGGA11012800.2708987768719008No Hit
GGCGGAAC10974130.26994755141591903No Hit
CGCAAGTG10902630.268188755964595No Hit
CTTGCGAT10762470.26474102490924434No Hit
ATATACAA10060830.2474817068607553No Hit
GATACACC10059270.24744333314181732No Hit
TCGCACGC9943300.24459064071538314No Hit
GGCCGCTC8932620.21972939055112947No Hit
ATAAGCCA8824610.21707250248543009No Hit
TCATTCAA7241370.17812711352942726No Hit
CTTACAAG6832580.16807147727003233No Hit
CGCGATTA6630690.16310527847747566No Hit
GACTTATC6090330.1498132126022668No Hit
GGCCTAAG4839690.11904929731214312No Hit
AATCTGTA4281820.10532651104039321No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)