FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27040116
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGACCG3787681.4007632215778956No Hit
TAATACCA2887351.0678023718537302No Hit
CAGGCGCA2713741.0035977656308872No Hit
CAGCTAAC2610410.9653841721684921No Hit
CTATCCTG2592750.9588531350974974No Hit
TCAACTTA2546060.9415861973373191No Hit
AACGCGCC2469970.9134465251554394No Hit
TCACGCCG2466000.911978336187611No Hit
CATGACTA2379510.8799925266592792No Hit
TTGACCGA2215380.8192938225560867No Hit
CTCGGCCT1998330.7390241964938317No Hit
GAAGCGAT1962850.7259029510080504No Hit
CGCGGTCG1930870.7140760786677098No Hit
TCGAGCAA1826170.6753558305740995No Hit
GGACGTTG1812050.6701339594844934No Hit
TAAGCAAC1797350.6646975922736426No Hit
GGTGAGAT1718790.6356444624719806No Hit
ACACGAAT1707870.6316060182582057No Hit
CATTAAGT1664400.6155299037918328No Hit
ATGCTAGG1633020.6039249239907107No Hit
GCCTGTGA1544410.5711550941571404No Hit
ATGCAACT1442020.533289132339521No Hit
CTGTAGCA1414300.523037697027631No Hit
GCAACAGC1403270.5189585725149995No Hit
CATCTCCG1367450.5057115879236612No Hit
TGCATCAA1360460.5031265398417669No Hit
TTCCTTAT1341870.49625156933498366No Hit
CTCTCGAA1328640.491358838845218No Hit
ACCGACAC1300640.4810038536816928No Hit
TTAAGGAC1291150.4774942533530551No Hit
GAGGTATA1277070.47228717509939677No Hit
GTTACCAA1258590.46545288489147013No Hit
CTCTATAG1243580.45990187320202325No Hit
TTGGAACG1237250.4575609069132691No Hit
CAAGCACT1234530.4565549940688124No Hit
CGCAAGTG1229990.45487600718872656No Hit
CTTGCCGA1194100.44160313513447946No Hit
CTGCGCTT1184320.4379862867452196No Hit
TCCGGTCT1168290.43205805773910144No Hit
TATACGGA1107460.40956185247134297No Hit
CTATGGAG1088720.402631408829755No Hit
TTGTGTAC1082190.4002164783612615No Hit
ATATTCGG1071130.396126259221669No Hit
TTCCGGTT1058410.39142213739023896No Hit
ATCATGGA1045640.3866995245138741No Hit
CCGCTGTA1007090.37244292886909214No Hit
AACCTTCT1002740.37083420795975874No Hit
ATACATGT990830.36642964105627357No Hit
TGCTTCTT987030.3650243216412237No Hit
GGCCAATA967860.35793485501319594No Hit
GTCTCCAC964380.35664787828572925No Hit
CCGGTCTT960620.3552573517066273No Hit
TTGAGAGT951240.35178843167684637No Hit
CTAACATA944950.34946225822404015No Hit
CGACTCTG889920.3291110141687262No Hit
GAGCGTTA883640.326788538924907No Hit
CAACAGCA875590.3238114806903935No Hit
CCTTACTG872880.3228092660549237No Hit
CGGACATC868070.3210304275321896No Hit
ACTGGTTA859750.31795351765502783No Hit
GATCGATT858700.3175652057113956RNA PCR Primer, Index 42 (100% over 8bp)
AGATGACT855780.3164853286871994No Hit
CGTTCATA846620.3130977692551319No Hit
AGCCACGC843010.31176271581083453No Hit
AATTGCTA840480.3108270689371303No Hit
CACACATG839790.31057189251702916ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
AATTATTG838200.3099838772881004No Hit
GTGTAGAT834120.3084750080214153Illumina Single End PCR Primer 1 (100% over 8bp)
CCAGCCAA826170.30553493187677155No Hit
CCTGGTGC814370.30117104527214306No Hit
CGACAGTT809270.2992849586887867No Hit
CGTCCGAA799340.29561263716472225No Hit
TACATGTC788960.291773896236244No Hit
GATACGAG788320.29153721086107764No Hit
ATAGGAGG785040.29032419831335043No Hit
GCCATCGC779720.28835675113228065No Hit
GTCCGCGG775530.28680720156673883No Hit
TCTACCGT755850.2795291262803754No Hit
CTGCTCAG746780.2761748507291906No Hit
CAGGAATC736500.2723730918905821No Hit
GGCTACGG734860.2717665856167185No Hit
AGAGATCG726110.2685306527531169No Hit
CTACAAGC724400.26789825901634445No Hit
TAATGATT723270.2674803614008165No Hit
TACGTACC722320.26712903154705403No Hit
GTCAGATG721740.26691453542580956No Hit
TCGTCTGG706750.2613709201543366No Hit
CTCCAATG700580.2590891252093741No Hit
TTAAGTGG692880.2562415042894047No Hit
CCGCGTAC691820.2558494941367855No Hit
ACGCATTA687480.2542444714364391No Hit
GGCGCCAT669570.24762097914076994No Hit
GCGGAGTA661790.24474377254890475No Hit
ACACTAGC656150.24265798268025182No Hit
GTAGAATG650210.2404612465419897No Hit
TGGTTCCA640500.2368702856156386No Hit
TCACCGAG638240.23603449038458266No Hit
AACTTGAA636460.23537620918490143No Hit
CCATTGAC636210.23528375396022708No Hit
CTGTAAGG632780.23401526827769525No Hit
AAGACACT629350.2327467825951634No Hit
AGCTGTGG626320.23162622527211052No Hit
TATGCCGC621410.22981040465950664No Hit
ATTGACCT620130.22933703390917407No Hit
TCAATAAC618740.22882298285998476No Hit
CAACTCGA606040.2241262574465287No Hit
CAATATGG605990.22410776640159383No Hit
TTATCGTC596130.22046133234043816No Hit
CCGGTAGG594030.2196847084531738No Hit
CCACATAT590940.21854196187619906No Hit
CAGCGTCT589950.21817583918648872No Hit
AGTCATGA574850.21259154361615903No Hit
AGATGCGA574410.21242882242073222No Hit
TGGCACAG573340.2120331140591261No Hit
CGTTCGCG561940.20781715581397656No Hit
GTCCGACT549420.20318699816228597No Hit
ACGGACGG544420.20133789366879934No Hit
CATATCTC538890.19929278409900314No Hit
TCTCTCAG538100.19900062558903223ABI Solid3 Adapter A (100% over 8bp)
TGATCCAT531780.1966633575092651No Hit
GGTCAACT526850.19484014047868728No Hit
GGTCGTAT525770.1944407339080942No Hit
ATGGCGCT523330.1935383709152727No Hit
TGCCTAGA521460.1928468058347087No Hit
GCAGATCT517740.19147107209155464No Hit
AAGAACGT512730.18961826938908102No Hit
CCAAGACG509940.18858646908171547No Hit
GTCATACA509680.18849031564805416No Hit
AATCGCGG508840.18817966609314843No Hit
ATTACGAC505480.1869370678735254No Hit
CGCTTACC504570.18660053085571082No Hit
TGAGCTCC504390.18653396309394532No Hit
CGCCTCAT503960.18637494010750544No Hit
GAGTCCTA502310.18576473562465487No Hit
AGACCATC502150.1857055642808633No Hit
TTGATGAG502030.1856611857730196No Hit
CGCTGAAG500550.18511385084294757No Hit
GCTCACGT499210.18461829083869313No Hit
CCGACCGC497450.18396740605698586No Hit
TGCAATCG496820.18373441889080652No Hit
TACCTGCA495580.18327584097642186No Hit
AGTCTGAT493180.18238827081954825No Hit
GCAGTTAA492700.18221075678817356No Hit
TAGGCATT490390.1813564705121827No Hit
CGCGCGCT489330.18096446035956354No Hit
CGACCTCG488830.1807795499102149No Hit
CGATCTAA488540.18067230184959265No Hit
CATCCTCA488450.1806390179687099No Hit
TTGTACTC485350.1794925731827482No Hit
ATTAATCA484130.17904139168633743No Hit
CGCGTCGG483570.17883429198306694No Hit
TCTCGAGT481280.17798740212505007No Hit
TAGCTGGT481210.17796151466214125No Hit
CCTTGGTT480000.17751403137471747No Hit
TAGCCACC479510.17733281913435578No Hit
GGCGTTGA474450.17546152538694731No Hit
AGGTTATC473040.17494007791978408No Hit
CACTGCTT470520.1740081292550668Clontech Universal Primer Mix Long (100% over 8bp)
ATCTAACC470410.1739674489562101No Hit
CTATTGGA467010.17271005790063917No Hit
CATACGTT461050.1705059253444031No Hit
ACGATAGA459890.17007693310191419No Hit
GTGTTAAG458540.1695776748886728No Hit
CGGTCGGA450620.16664869337098998No Hit
CCATAAGA449170.16611245306787886No Hit
GGCAACAC446900.16527295962783592No Hit
AGTGGCAA446710.16520269365708343No Hit
ATCTGTCT439550.16255477602241056No Hit
GCGCCTAA438640.162218239004596No Hit
TACTCCGG437110.16165241302958908No Hit
CGTAGTTC434620.16073155899183272No Hit
AACGCTGG434110.16054295033349708No Hit
CAGAGGAT433830.16043940048186184No Hit
CCAGGATA433790.16042460764591396No Hit
CTGTTCGC432510.15995123689558136No Hit
CACATTAT430630.1592559736060304No Hit
ATTGCCAG430060.1590451756937729No Hit
TGCTATAC425380.15731441388786943No Hit
GTCACAAT421850.15600894611546784No Hit
TACTGTTG421020.15570199476954907No Hit
CCTATACC420960.1556798055156272No Hit
TGGCTGTG419660.1551990383473207No Hit
AATCCAAG418850.15489948341937587No Hit
AACACGTA416890.1541746344579291No Hit
CAGTACCG414030.15311694668765474No Hit
CTGATTAC413510.15292463982033214No Hit
TGTCCTGC410820.15192982160283633No Hit
GTGTGTGT410810.15192612339384934No Hit
GAACAGAC410710.15188914130397962No Hit
ACCTATAA406710.1504098577091903No Hit
GTGTCGGC405820.15008071710934967No Hit
TACGAGCG401990.1486643030673389No Hit
AAGTGAGA401850.1486125281415213No Hit
GGACACAT401730.14856814963367762No Hit
AATTAACA400680.14817983769004542No Hit
GGAATATC400210.14800602186765766RNA PCR Primer, Index 37 (100% over 8bp)
TAACCTCT398540.14738842096683313No Hit
TGGAAGAT397290.14692614484346148No Hit
CAGATAGT391030.1446110660176162No Hit
TGGCCTAT390060.14425233974587978No Hit
TTGCTCTA389230.14394538839996102No Hit
GAGACTGA389150.14391580272806523TruSeq Adapter, Index 15 (100% over 8bp)
GCATCGAA389020.14386772601123457No Hit
GCACACCA381310.14101640688227818No Hit
GCGAGATA381170.14096463195646053No Hit
AAGCTCCA379750.14043948628031033No Hit
TGTAGCAC372510.13776198297374168No Hit
CACGATAC372260.13766952774906735No Hit
ATAATATG369430.1366229346057539No Hit
TGATAAGG367720.1359905408689815No Hit
AGACTCAA366590.1355726432534535No Hit
CCGTTACT365050.1350031190694596No Hit
ACTGTCAT363440.13440770742255692No Hit
GCTAGTCT362110.13391584562728948No Hit
GCTGGAAT361000.13350534442973544No Hit
TGCCGTAG360820.1334387766679699No Hit
TCCGACTT359150.13282117576714536No Hit
TTACAGCG358900.13272872054247103No Hit
CGGTTGTT356440.1318189611316756No Hit
CTTCTAGA350090.1294705984249476No Hit
TAGGATCA348920.1290379079734717No Hit
GTTCGGAC348630.1289306599128495No Hit
TTAATACT348320.1288160154342533No Hit
ACACTGTG347370.12846468558049084No Hit
CAGCACAA346460.12812814856267626No Hit
GAAGGCAG345680.12783968826169237No Hit
GGTCCGTG344940.12756602079665635No Hit
GGCGTGTC344290.1273256372125031No Hit
CAATCGTA343070.12687445571609232No Hit
ATATTACA340940.12608673720186703No Hit
TCGGATTG340890.12606824615693218No Hit
ACTGAGGC340390.1258833357075835No Hit
TCTAGTGA339170.12543215421117276No Hit
GTTCAGGA338530.12519546883600646No Hit
AAGCAGGA338000.12499946375969688No Hit
ATGCGGCA335700.12414887569269305No Hit
TGCAGTGT333540.12335006255150681No Hit
CGCCGGTA332070.12280642583042173No Hit
CAGTGGTC332060.12280272762143477No Hit
CGAGTTCT331880.12273615985966924No Hit
AGCAACTA330630.12227388373629758No Hit
GGCCGTCA329390.1218153058219129No Hit
GTAATTCC329010.12167477388040791No Hit
ATCATCTC327540.12113113715932283Illumina PCR Primer Index 7 (100% over 8bp)
CGTGTGGT327200.12100539805376574No Hit
AATCAGCT322620.11931161833773198No Hit
AGGACCAG320930.11868662101893351No Hit
GGAGAACC319150.11802833981925226No Hit
GCTGCATG318470.11777686160813806No Hit
AGAACGGT318020.11761044220372427No Hit
AAGCCTGT315790.11678574159962923No Hit
AACGGATA315390.1166378132401503No Hit
TGGTTAGT314520.11631606905828362No Hit
GACGCCTC313310.11586858577085986No Hit
AATGCGAA312660.1156282021867066No Hit
TATACTAT311760.115295363377879No Hit
TTCTTGCC310870.11496622277803838No Hit
AGCGAAGA308410.11405646336724296No Hit
TCTCATTC308380.11404536874028202No Hit
AACCAACG307850.11384936366397244No Hit
CAAGGTCC307490.1137162281404414No Hit
ATTCTGGC306220.1132465555990958No Hit
ACCAGGCC300940.11129390125397391No Hit
GACATCTA300870.1112680137910651No Hit
AGAACACA299960.11093147677325053No Hit
GAGTGAAC296890.10979612661424973No Hit
GATCTTCC296540.10966668929970567No Hit
TGATTCGC296000.10946698601440912No Hit
CCAGATTC293160.1084166946621087No Hit
AGCGTTAT291000.10761788152092247No Hit
TACTCAAT289790.1071703982334987No Hit
CCATCTCC289520.10707054659085041No Hit
ATTCCACA289260.10697439315718912No Hit
AGATGGAC289180.10694480748529332RNA PCR Primer, Index 16 (100% over 8bp)
GCAGTGGT288930.106852352260619Clontech Universal Primer Mix Long (100% over 8bp)
GATTACGC288920.10684865405163203No Hit
CTCTTAAT288430.10666744181127034No Hit
GATATTGT286970.10612750329917224No Hit
CGTAATGT286680.10602025523855No Hit
CTGGATGC285910.10573549314655308No Hit
GGAACCTG285670.10564673613086573No Hit
ATAGGTAT282880.10461493582350016No Hit
ACGCTATT278770.10309497192985415No Hit
GGCTCTTA278570.10302100775011468No Hit
CTGTCATT278480.10298772386923191No Hit
TTAGCAGT278160.10286938118164878No Hit
TGAGTAAG277090.10247367282004265No Hit
AGGCGGTT275850.10201509490565795No Hit
AGAAGTAG275340.10182648624732231No Hit
GGTAGCGA273950.10131243519813302No Hit
AAGGCCTG273750.10123847101839356No Hit
ATAACTGA271900.10055430235580351No Hit
GCGACAAG271550.10042486504125943No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)