FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400863926
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGATC44212581.1029323701230227No Hit
GTGTGTGT39665550.9895016095811026No Hit
CTGCCTTA35635810.8889752279680063No Hit
CCACCAGG34881370.8701548764455298No Hit
TATGCCGC34563570.8622269991937364No Hit
CGGTTGTT33837700.8441193583480494No Hit
CCTGGTGC32948870.8219464976252316No Hit
CGTAGTTC30804800.7684602679862992No Hit
TCTGTGTT30634020.7641999694429974No Hit
TCTCTCAG30507350.7610400442967273ABI Solid3 Adapter A (100% over 8bp)
CACGCCGT30412980.7586858788585531No Hit
GGCTACGG30215360.7537560264277808No Hit
CCAAGACG29211710.7287188520924679No Hit
TTGACTCT28930510.7217040028690433No Hit
ACTGTCAT28913130.7212704392861732No Hit
TGGCCGCA28840890.7194683315055892No Hit
CCTGTGCG28499440.7109504784922951No Hit
AATGTAGG27497340.6859519706445224No Hit
AACGCGCC27116140.6764425093217292No Hit
CTTCGCAA26863510.6701403707750944No Hit
ACGACGAT26858090.6700051627993086No Hit
ACTGGTTA26758600.6675232732216467No Hit
CTCATAGG26538150.6620239008485888No Hit
CACACCAC26443570.6596644967250059No Hit
AACCGTTC26159300.6525730629101308No Hit
GAGCGTTA26140850.6521128069777973No Hit
GTGCCACG26125150.6517211528781963No Hit
CGACGCCT25842860.6446791123828888No Hit
ACAGTGCC25702130.6411684447754473No Hit
CATTCTTC25695260.6409970649242207No Hit
GGTTAATG25665380.6402516748289294No Hit
TGCTATAC24862690.6202276729685074No Hit
GCGTCGCT24678500.6156328469426805No Hit
AGCGTTAT24678210.6156256125675923No Hit
CTTCTGCT24612320.6139819126553183Illumina Single End Adapter 1 (100% over 8bp)
CTGCGCTT24502120.6112328501218142No Hit
CCAGGATA24373590.6080265251904957No Hit
CGGTCGGA24197220.6036267778308393No Hit
ACCAGGCC24034080.5995570676519293No Hit
TTAAGTGG24017000.5991309879053572No Hit
TTAGGTCA24013580.5990456721715588No Hit
TAGGTGAA23686220.5908793100030657No Hit
GCTGACAA22926320.5719227526599637No Hit
GTTCACTG22829870.5695166992901227No Hit
CACTTGCT22824400.5693802440082872No Hit
GCATCGAA22722560.5668397310462903No Hit
CTCAATTC22498650.5612540450945941No Hit
ACGCCTCG22270600.5555650822019839No Hit
TTGATGAG22177680.5532470886392507No Hit
TGGTTAGT21882840.5458919743254722No Hit
TGTAGCAC21537460.537276083056673No Hit
GACGCAAG21406150.5340004079089921No Hit
TTATACGT21361940.5328975398998612No Hit
AGTGGCAA21346550.5325136190977684No Hit
ACAAGTGT21307090.5315292451633575No Hit
CGGTATAT21274900.5307262295285707No Hit
ATACGTTA21239340.5298391454660353No Hit
CATCAGTG21229110.5295839466482699No Hit
CACGATAC21200780.5288772230405188No Hit
CTATCGCT21188330.5285666438341474No Hit
CCATAAGA21160270.5278666556790645No Hit
GCCACTGT20720920.5169065774204885No Hit
GCACGTCC20600560.5139040622976886No Hit
GTCACAAT20558570.5128565746771636No Hit
GCGCTCAT20547370.5125771781220344No Hit
ACAACCTT20546080.5125449976259525No Hit
TCTCTGCC20529650.5121351328580263No Hit
CGACTTAC20516140.5117981107634015No Hit
TTGCAGAA20435910.5097966834760781No Hit
TGCGGATT20401670.5089425282932543No Hit
AATATTCG20329640.5071456591980791No Hit
GTGATGTC20179560.5034017453593467No Hit
TCAACTTA20062130.5004723223710581No Hit
GTGTAGAT20035140.49979902656543856Illumina Single End PCR Primer 1 (100% over 8bp)
TGGTTCCA19771340.49321823984730423No Hit
GCTAGTCT19721590.49197717032786836No Hit
CATGTATT19712320.4917459197862568No Hit
AGGTCTTG19432990.4847777198090905No Hit
GCGGCTCC19387980.4836548949031647No Hit
CTCTAGTT19274670.48082824993337014No Hit
ATGGAGAG19040050.47497539102583153No Hit
TAAGTCCT18979840.4734733850808017No Hit
TCTTAGTA18888290.47118956770382975No Hit
GATTGAGG18783130.46856623362013367No Hit
CTCGGCCT18643790.46509024112087355No Hit
TCATCATG18513880.46184949054258373No Hit
TTGCTCTA18497210.46143363870561904No Hit
AGCAGGAA18339560.45750088273096445Illumina Paired End Adapter 2 (100% over 8bp)
TTCGACAA18209740.45426237730356406No Hit
ACGATAGA18183390.45360504701538046No Hit
ACCTATAA18118040.4519748180084431No Hit
ATAGCCTA17975090.44840877001239565No Hit
TGTGTCGA17950840.44780382657829876No Hit
CCTTGTAG17886850.4462075242959128No Hit
TATGAGGT17836590.4449537322547702No Hit
CCTAACCT17801270.44407263526127316No Hit
TATCACAT17761460.44307953018451457No Hit
GATGGTAC17714320.44190357004087216No Hit
GCCAACTG17588360.4387613566405075No Hit
ATTACGAC17561210.43808406945552886No Hit
AATCAGCT17494850.4364286448663879No Hit
AACGGCCG17272420.43087987917376236No Hit
AGTGCTCA17167520.4282630310815247No Hit
ACTATTAC17120190.42708233117489347No Hit
TGCGCCTG16985440.4237208413709943No Hit
CACCGGAG16922730.42215647012348023No Hit
TATGCTTA16666760.41577101153272644No Hit
CAACTCGA16629780.4148485039783799No Hit
CATATCTC16513890.4119574980164216No Hit
TTCCTTAT16364800.408238280837473No Hit
TCTGATAT16315900.40701841552088175No Hit
ACGATTCT16205460.40426336591833906No Hit
CCTTGGTT16183430.40371380287284814No Hit
TACCTGCA16131510.40241860027085596No Hit
CGGCTTGA15842870.39521815190723847No Hit
AGCAACTA15715930.39205149130829997No Hit
GACATCTA15613560.3894977569021763No Hit
GTAAGAGA15575960.3885597827528137No Hit
ATTAGGCT15519880.3871608042874878No Hit
TATTGACC15445810.38531304510548553No Hit
GAGAAGCT15437810.385113476137536No Hit
AATCCAAG15236950.38010279827474425No Hit
CTAGGCAC15027070.3748671064005894No Hit
ATGTAATA14848310.3704077378117581No Hit
CAGCACAA14729730.3674496267843268No Hit
GCTACCTC14691750.36650217310898664No Hit
CTGTGTTG14635440.3650974570358322No Hit
GAGTTCCT14486360.3613784893180934RNA PCR Primer, Index 1 (100% over 8bp)
ATGAGACG14399620.3592146627831011No Hit
TTCCGAGC14306560.3568931767634287No Hit
CGCGGTCG14123390.3523237957810152No Hit
AGAGATCG14078360.35120047195266957No Hit
AACAGAAT13895580.3466408199574436No Hit
TGGCACAG13690070.34151414263203117No Hit
TGCCGTAG13689500.3414999233430648No Hit
CTTCGACG13668480.3409755558797775No Hit
AGTCTGAT13652550.3405781641723481No Hit
ACTTAAGG13441250.33530704880638224No Hit
GCTTATAC13316860.3322040008159776No Hit
ACACGAAT13233760.3301309781614023No Hit
GTAGTGAC13179650.32878114355443394RNA PCR Primer, Index 43 (100% over 8bp)
GCACACCA13146550.327955426949543No Hit
CAAGGTCC13096870.3267161036585766No Hit
CTGATTAC12964410.32341174047175303No Hit
TGACGACC12875500.32119378085420436No Hit
GGTCTCGC12777670.3187533018373921No Hit
ACCGACAC12577140.3137508561945282No Hit
CCGTTACT12404520.3094446567885981No Hit
TGAGATAA12173620.303684597451156No Hit
TGAATTGA12082490.3014112574450014No Hit
CATGACTA12039430.3003370774750133No Hit
CCATGCGG11931430.2976428964076952No Hit
CCTATACC11751200.2931468570210032No Hit
GCGATCCA11729730.29261126380326874No Hit
GAGAATTC11669900.2911187373842165Illumina Small RNA Adapter 2 (100% over 8bp)
TCAATCGG11636880.29029501646900496No Hit
AAGAACGT11561000.2884021048080041No Hit
AACAATAG11532630.28769438335541325No Hit
ATTAGTGC11507340.2870634959554829No Hit
AGAATGTA11503960.28697917806652423No Hit
TACCTCGC11447030.2855589953983537No Hit
TTCAGTAA11420910.28490740271799864No Hit
CGTATCCA11412820.28470558859915973No Hit
GACAAGGC11404850.28450676801484004No Hit
GCAAGCAC11329350.28262333587981675No Hit
AGACTCAA11102880.2769737878583767No Hit
TCTCATTC10800940.2694415560855431No Hit
CGAGTTCT10628350.26513610506324287No Hit
CGATCTAA10624840.265048544178555No Hit
AAGATGGC10502250.26199039920593903No Hit
TTCTATGA10325480.25758067339788515No Hit
ATCTAACC10223400.2550341733668497No Hit
GTCCGCGG9997530.24939959301800582No Hit
TACAAGAA9925960.24761419913848773No Hit
TCCACAGG9859020.24594430579917037No Hit
AGTAACCG9761030.24349983540299905No Hit
GTATATCA9740310.24298295177600987No Hit
CCTCCATC9441300.23552381213768786No Hit
AAGCATCC9413030.23481858529719635No Hit
TCGGAGCT9224990.23012771670554363No Hit
CACCACGG9115250.22739012938769648No Hit
ATTCCACA8879460.2215080835185953No Hit
AAGGTGTT8553170.21336841369956547No Hit
GTCGACCG7236270.18051686696298033No Hit
TATACGGA7228960.1803345108185165No Hit
ATACATGT6980920.17414687496724263No Hit
AGGACCAG6896160.17203244175181778No Hit
GGACGTTG6753870.16848285819562622No Hit
CGCCAGCC6431650.1604447190890407No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)