FastQCFastQC Report
Thu 23 Mar 2023
EGAF00001836186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406264159
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCCGT54407561.3392163397805417No Hit
TTAGAGAT41848241.0300746219653603No Hit
AGATGGAC38316770.9431491592641329RNA PCR Primer, Index 16 (100% over 8bp)
CACTGCTT33114050.8150866687701092Clontech Universal Primer Mix Long (100% over 8bp)
CAAGACGC32262210.7941190303228299No Hit
TGATCCAT32179080.7920728247159996No Hit
GTTGCCTT31193160.7678048705251403No Hit
CTGACTGG31016950.7634675447705442No Hit
CCATCTCC30895470.7604773720637267No Hit
ACAGCGGA29950170.7372092599485253No Hit
GATTACGC28830200.7096417284498877No Hit
ATCGGCGC28608690.7041893646345505No Hit
GTGCGGTG28310740.6968554663961878No Hit
ATCATCTC28275810.6959956809776074Illumina PCR Primer Index 7 (100% over 8bp)
TTCCGGTT28126620.6923234397351797No Hit
ATTGACCT27974020.6885672629566124No Hit
TCGCAACA27184180.6691257251664182No Hit
GGCGTTGA25980020.6394858966626195No Hit
CTAATGAT25854760.6364026810447732No Hit
AATTAACA25849880.6362825621543445No Hit
TCTAGTGA25698470.6325556766625825No Hit
ATCGCGTG25644870.6312363380300058No Hit
TTAATACT25515830.6280600795011306No Hit
CGCACCGA25385840.6248604371718649No Hit
TCTATGAA24908490.6131106928386464No Hit
GCGAGATA24779020.6099238500632786No Hit
TCGGCAGC24572280.6048350428077018No Hit
AGTGTCTC24539410.6040259633141796No Hit
ATAATATG24494080.6029101868176365No Hit
TAGGATCA24402330.600651803990418No Hit
TGTGAACA23622020.5814448426399337No Hit
ATCGCTAA23581250.5804413083852666No Hit
CAGGCGCA23525080.579058710419001No Hit
CCGAAGGT23412000.5762752997366918No Hit
TTCTTGCC23405510.5761155514582323No Hit
TGACGGAA23400160.575983863740242No Hit
TGTCCTGC23340070.5745047768292058No Hit
GGAAGAAT23240390.5720512008050407No Hit
ACAGGCTG22719770.5592363859003373No Hit
CGCTTACC22711890.5590424234297272No Hit
GCGCGAGG22209300.5466714084419148No Hit
TACTGTTG22178210.5459061428059668No Hit
GGCCGCTC22140230.5449712830808685No Hit
GGCTCTTA22099570.5439704564241415No Hit
AGAGCCGC21975450.5409153013667641No Hit
CGTGTGGT21904960.5391802233777654No Hit
AAGTGAGA21869580.5383093614221578No Hit
ATAACTGA21862140.5381262293433076No Hit
TTACAATT21748400.5353265730733584No Hit
CTGGATGC21611320.5319524137495969No Hit
GCCATGCG21506600.5293747805107268No Hit
TCCGACTT21439130.527714038392444No Hit
TTACAGCG21366670.5259304697857927No Hit
TTAACCAG21365960.5259129934718164No Hit
CGAGAGGA21254230.5231628124005888No Hit
CTATCCTG21066990.518553988416192No Hit
GGACACAT20863630.5135483782609531No Hit
CGCCTCAT20819580.5124641083586209No Hit
CTTATGTA20797630.5119238194969593No Hit
GCGTACGA20735970.5104060877789616No Hit
AACGGATA20699410.509506180681816No Hit
TGCAGTGT20590500.5068254125759589No Hit
GACCACTT20585400.5066998784896504ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
ACCTCGCA20534260.5054410915928226No Hit
AATCGCGG20494250.504456264378468No Hit
GAGTGAAC20392170.5019436134901577No Hit
ACTCTTGT20280590.49919712459793925No Hit
TCACGCCG20203010.4972875296144447No Hit
ATGTGCAT20200930.4972363313988522No Hit
ATTAATCA20197110.49714230390675446No Hit
CAGGCTAG20144060.4958365032638776No Hit
TCTGACCG20019000.49275821055137675No Hit
TAATACCA19916130.49022611418695194No Hit
TCCTTACA19804900.487488240379088No Hit
ACACTAGC19795460.4872558792467834No Hit
TAACCTCT19780990.4868997070450411No Hit
GACGCCTC19779360.4868595853664758No Hit
TAATAGGC19775930.48677515753980155No Hit
TCCAACCA19696040.48480870299956735No Hit
TAGGCATT19067360.4693340423367251No Hit
GGCACTCC19066840.4693212427828269No Hit
TACTGCAC18964920.466812530218793No Hit
GGTCCGTG18909620.46545134689077994No Hit
CGGCACTC18796900.46267679743809254No Hit
CCGGTAGG18778060.46221305975455246No Hit
AATCTGTA18763660.4618586105696811No Hit
GGTTGGCC18560310.4568532465597094No Hit
TCGATTGC18500510.4553812978614242No Hit
GTTATCCG18464330.45449074428443487No Hit
GTGTCGGC18312990.4507655818095438No Hit
CTGAACTA18296620.45036264200701986No Hit
ATCCTCCG18101100.44555000974132203No Hit
TGGCTGTG17887180.4402844701838441No Hit
GCACCACT17764240.43725836026800485No Hit
GGTAATTA17695450.4355651269744422No Hit
AGTTACAT17665640.43483136793270505No Hit
GACTTATC17489000.4304834579316163No Hit
GTACTATA17458140.42972385363681564No Hit
ATGGTCAA17447990.42947401619053477No Hit
CGCGTCGG17444120.42937875797210057No Hit
TCGGATTG17410770.42855786350574926No Hit
CGTCGTGG17243550.4244418223464305No Hit
TTATGCTA17006190.41859931828246755No Hit
GCTGGAAT16989260.4181825943449764No Hit
GATGGCGT16923200.4165565587093791No Hit
ATTCGATC16910920.4162542923211693No Hit
GTTGCAAC16595890.4084999779663064No Hit
GGAGTGCA16219470.399234577815662No Hit
CCACTACA16130090.3970345314167869No Hit
TTCACGGT16090550.3960612730299943No Hit
TACTCCGG15968120.3930477165227858No Hit
TGGAGTTA15624120.3845803193286366No Hit
GGCTGAGT15619870.3844757075900461No Hit
TATCAAGA15567020.3831748298525148No Hit
CTTGCCGA15550000.3827558906076182No Hit
TCACCGAG15484170.38113551631316805No Hit
ACCTAGGT15446900.38021813290204615No Hit
TGTGGAGC15441770.3800918603799357No Hit
AGCTTCAC15346140.37773797318901564No Hit
GAGGTATA15140420.3726742727507006No Hit
GACTCGGT15132820.37248720234757404No Hit
GTAGCTTC14997730.36916202593199954No Hit
CGAGGAGT14903130.3668334917036085No Hit
GTGGTACC14870850.36603893478085525No Hit
AAGCAGGA14863470.36585727957360864No Hit
ATATACAA14770780.36357575909126655No Hit
ATAGGTAT14757360.3632454321425878No Hit
TCTCCACG14602130.359424519158728No Hit
CGGAGAGA14548240.3580980423134003No Hit
GATACACC14472470.35623299962328203No Hit
TTAAGGAC14305100.35211326628495426No Hit
TACTCAAT14213070.3498479913902521No Hit
ATAGTTGC14096190.3469710455063795No Hit
GGATTGTG14093390.3469021248315434No Hit
GGTAGCGA14059730.3460735998619066No Hit
TAATTAGA13992640.3444222112637802No Hit
GATATTGT13986830.3442792008634953No Hit
CGCAAGTG13853160.34098897707587345No Hit
ACTCCGTT13837720.3406089287832058No Hit
CTTACAAG13814930.3400479637190934No Hit
ATAAGCCA13784460.33929795908971633No Hit
AACCAACG13648360.33594792200214735No Hit
CGTGATCC13600530.3347706091887865No Hit
CTTGCGAT13598480.33472014940899575No Hit
CGATGCTC13467520.3314966309888045No Hit
ACGTGGTA13354180.32870682052954614No Hit
GCATGTAT13286520.3270414016511853No Hit
GCAGCTGG13063610.32155457749843No Hit
GTTACCAA13059610.3214561193915213No Hit
TCGTGAAG13057220.3213972906726434No Hit
GGCCGTCA12932680.3183317975140406No Hit
TGAGCGTA12894340.3173880765593206No Hit
GGTGTACG12818380.3155183571091242No Hit
AGGTTATC12802750.31513363205637834No Hit
TAGATCCG12798910.31503911227374604No Hit
GGAATATC12738360.31354870268041535RNA PCR Primer, Index 37 (100% over 8bp)
TGTGAGTC12710300.31285801906045074No Hit
TTCGCATA12680140.3121156449343591No Hit
AGGCCAGA12475690.30708320494498753No Hit
CCAGCGTG12390320.3049818627982883No Hit
TAACGGTC12359720.30422865828043666No Hit
GTCTTGTA12256440.3016864699600537No Hit
GAACAAGT12229100.3010135087993327No Hit
CATCTCCG12146670.2989845333612114No Hit
CTTAACAC12127730.2985183342249987No Hit
GCCTTAGG11887440.2926037095977251No Hit
AGCAAGCT11869350.29215843280923043No Hit
ACTCATAG11859680.29192041033577865No Hit
CCTACTCG11607060.2857022885939589No Hit
ACTACATA11598350.28548789606616515No Hit
ATTCTGGC11561530.28458158919207044No Hit
TCGCACGC11399290.2805881283758531No Hit
GCGGAAGT11303000.27821799559729316No Hit
TGTCTTAA11121340.273746520672034No Hit
CCGACACA10688530.26309310735924407No Hit
GAGTATAA10596590.2608300477719473No Hit
GGACCGGC10538680.25940462052917646No Hit
CGAGCCTT10419410.25646884592642594No Hit
AGCGAAGA10242870.2521233973780099No Hit
TACACACA10111160.2488814180627733No Hit
AGTGGATG9813680.24155908865197234No Hit
CGTAATGT9750730.24000960419449652No Hit
GGCGGAAC9096940.2239168727655348No Hit
GGCCTAAG8967050.22071969188894167No Hit
TCATTCAA8797310.21654162212227046No Hit
CAGTGGTC8030950.1976780334196303No Hit
CGCGATTA7413750.18248594752361605No Hit
GACCTGTG7044860.17340589475922735No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)