FastQCFastQC Report
Mon 4 Sep 2023
EGAF00001836190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399027540
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCGTG41277361.0344489004443154No Hit
TCCGGTCT38088300.9545281009927284No Hit
CACACCAC36819450.9227295439307271No Hit
GTCAGATG36091500.9044864422139886No Hit
TTCGACAA35763560.896267961855465No Hit
TATGCCGC34933500.875465888895789No Hit
CCAGCGTG34908520.8748398669425173No Hit
CCACTACA34812560.8724350203998451No Hit
TCCGCGAT34416500.862509389702776No Hit
GCAGCTGG34076900.8539986989369206No Hit
ATCGGCGC34015780.8524669750864815No Hit
CACGCCGT33650350.8433089605795129No Hit
GGTCTCGC33154010.8308702201356828No Hit
GGCACTCC32970700.8262763016307095No Hit
GCACCACT32885860.824150132594858No Hit
ACCTCGCA32742950.8205686755355281No Hit
TCAATAAC32469590.8137180205656983No Hit
CGTGATCC32444790.8130965095792636No Hit
GCTTCTCA32339880.8104673677410837No Hit
TAGGATCA32141710.805501043862787No Hit
GCGTCGCT31974670.801314866638027No Hit
CTGTCATT31243970.7830028473723895No Hit
TGTGGAGC31117000.7798208614874051No Hit
GTACTATA31107470.7795820308543114No Hit
ACTGAGGC30476770.7637761042759104No Hit
GATTACGC30376330.76125898478085No Hit
TTACAGCG29709990.7445598867687178No Hit
CTGACTGG29636180.7427101397562684No Hit
GGCTCTTA29536880.7402215897178426No Hit
TGATCCAT29028020.7274690864695705No Hit
GCCTACAT29017970.7272172241545033No Hit
GTAGCTTC28852270.7230646285717522No Hit
GCACACCA28848740.7229761634998928No Hit
ATGAATTG28835160.722635836112966No Hit
GTTGCCTT28237150.7076491512340226No Hit
TTGACCGA28179530.706205140627637No Hit
TGAGCTCC28168010.7059164387500672No Hit
TTCAGTAA28077310.7036434126827437No Hit
CGGTCGGA27690470.6939488437314377No Hit
CCTTGTAG27588350.6913896218792317No Hit
CGTGTGGT27546810.6903485909769537No Hit
TGAGATAA27487660.6888662371524532No Hit
ACCGAACT27185170.6812855573828313No Hit
TCCAACCA27146950.6803277287577694No Hit
ACTTGTCC27115620.6795425699188582No Hit
TGGTTCCA26722380.6696876110355692No Hit
CTGTGTTG26597080.6665474768984616No Hit
GGTAATTA26535460.6650032225845864No Hit
AATCTGTA26344750.6602238532207576No Hit
CATACGTT26267950.6582991740369599No Hit
CTAGTCCG26227640.6572889680747349No Hit
TTAGAGAT26181830.6561409270147118No Hit
GTATCTAG26178290.6560522113335836No Hit
TCGAGCAA25893600.6489176160622898No Hit
AATCAGCT25863450.6481620291170881No Hit
CGAATCGT25670680.6433310342439021No Hit
TCACGCCG25663390.6431483400870025No Hit
CTTACAAG25542090.6401084496573846No Hit
ACCTATAA25391180.6363265051830759No Hit
AACGGATA25330440.6348043044848484No Hit
TCGGAGCT25199780.631529843779705No Hit
ACAGGCTG25147770.6302264249730732No Hit
AGCTGTGG25110640.6292959127583024No Hit
TACCTCGC25010130.6267770389983609No Hit
CCATTGAC24841340.6225470051515742No Hit
ACTCATAG24719850.6195023531458506No Hit
GCACTCTC24700290.6190121614162271No Hit
TTGACTCT24587280.616180026070381No Hit
TTATGACG24566200.6156517417319115No Hit
TTGAGAGT24561620.6155369626868361No Hit
TTGTACTC24554520.6153590301060423No Hit
TGATTCGC24177370.6059073015361296No Hit
GCGCGAGG24138410.6049309278251822No Hit
TCGTCGAC24119090.604446750718008No Hit
AGAAGTAG23672010.5932425115319109No Hit
CATATCTC23581890.5909840208021733No Hit
GCCATCGC23392720.5862432452657278No Hit
CTGAACTA23379830.5859202099183429No Hit
GCGGAGTA23294420.5837797561541742No Hit
CATAATAA23241520.582454033122626No Hit
AGTTACAT23191160.5811919648453338No Hit
GGCGTGTC23142950.579983777560817No Hit
TTAGCAGT23109740.5791515041793858No Hit
GTAATTCC23064530.5780184996754861No Hit
AGACCATC23039830.5773994947817387No Hit
CGTATGAC23011030.5766777400878145No Hit
CTTGCTTG22781020.5709134762979016No Hit
CCGGCTGA22571120.5656531877473921No Hit
AGCTCACG22539850.5648695325640932No Hit
CGGAGAGA22534090.5647251816253084No Hit
TGGCACAG22480020.5633701373093196No Hit
CAATTAAG22418420.5618263842139818No Hit
ATAGGAGG22267050.5580329117133118No Hit
TGTCGGCT22226520.5570171923471748No Hit
CATTAAGT22164370.5554596557420574No Hit
ATGGAGAG22150360.5551085521565755No Hit
ATTCTGGC22069950.5530934030267686No Hit
TCCTGGAA21951380.5501219289275121No Hit
AACAGTGA21782120.5458801164450955No Hit
ATACTTAG21696200.5437268816082219No Hit
TCCACAGG21600340.5413245411582368No Hit
TGATAAGG21561190.5403434058711837No Hit
GTGTTAAG21553070.5401399111449801No Hit
CCGGTAGG21151140.5300671728071702No Hit
CACTCTCG21053110.5276104501458722No Hit
TGCTGCCG20947880.5249732888110931No Hit
CTAACATA20859770.522765170544369No Hit
AAGTGAGA20768130.5204685872057854No Hit
TAGCCACC20211000.5065063930173842No Hit
TGTACGCC20156050.5051292950857478No Hit
GGCCGTCA19907950.49891167912871376No Hit
TATAATCC19859210.4976902095529547No Hit
CTGGATGC19854440.4975706689317735No Hit
CTTATGTA19750730.49497160020584047No Hit
TCCGGCGA19749220.4949337582062631No Hit
CAGCGTCT19719820.49419696695621557No Hit
TACGAGCG19679220.49317949332519756No Hit
ATTAATCA19578890.49066513053209304No Hit
CTCTCGAA19116740.4790832231780293No Hit
AAGTGGAG19002640.4762237714218924No Hit
TACAGCCT18867450.47283578471801724No Hit
ATAACTGA18609330.46636705827372216No Hit
AATCGCGG18330120.45936979688168894No Hit
AGCCATTG18213260.4564411769673843No Hit
ATCATGGA18150520.4548688544154121No Hit
CCTTACTG17912660.4489078623495511No Hit
ACGGACGG17895290.44847255404977815No Hit
CGTTCGCG17421390.436596180804964No Hit
ATAAGCCA17120410.4290533430349194No Hit
TGGCTGTG17039730.4270314274548569No Hit
TAAGACTG16921090.4240581990907194No Hit
TTACCTAC16313980.4088434597772374No Hit
TGTCTTAA15724680.39407505557135225No Hit
AGGTAGCC15172810.3802446818582998No Hit
CGGCCAAG14675140.36777261038172954No Hit
CTATCCTG14294190.3582256502897018No Hit
CACGGCAA14166510.35502587114663814No Hit
GGCTACGG8636110.2164289211717066No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)