FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401960520
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTTACT43011221.0700359328821647No Hit
TTCTTGCC39699330.9876425177278605No Hit
GGCGGAAC38353650.9541646030311635No Hit
GCAGTGGT38258440.9517959624492475Clontech Universal Primer Mix Long (100% over 8bp)
GATGGCGT35437800.8816238967946404No Hit
CTTGCGAT35244580.8768169570484186No Hit
GGCCGCTC35170340.8749700094924746No Hit
GTGCGGTG35072330.8725317103281685No Hit
GTGGCGAA34759210.8647418905717408No Hit
GCAGATCT34447690.8569918757195358No Hit
GATATTGT33477950.8328666208313195No Hit
TAGATCCG32661870.8125641294324129No Hit
GCATGTAT32361510.805091753787163No Hit
TAGTCGTG32311730.8038533237045269No Hit
TTATCGTC32283180.803143054944799No Hit
ATAGTTGC31921610.7941478929323705No Hit
TATCACAT31379490.7806609962590355No Hit
ACAGCGGA31329820.7794253027635649No Hit
TAGGTGAA31314160.779035712263483No Hit
AGTGTCTC31304580.7787973803994482No Hit
AGTGCTCA30650030.762513442862498No Hit
TCGATTGC30537860.7597228702958191No Hit
CTCACGCC30425850.7569362782195624No Hit
GCTGTAGC30197650.7512591037547668No Hit
GCGAGATA29952700.7451652216988872No Hit
ACTCCGTT29886920.743528742574022No Hit
CACAGGCA29780140.740872262778444No Hit
GACAAGGC29592250.7361979231194148No Hit
ATTGACCT29392000.7312160905752634No Hit
GTGGTCGT29102070.7240031931494167No Hit
GGTAGCGA28751930.7152923874215309No Hit
GCCTGGTC28538480.7099821644175404No Hit
AGAATGTA28470520.7082914511106713No Hit
ACTATTAC28245360.7026899059638991No Hit
GGAAGAAT27931290.694876452045589No Hit
GACAGTCG27928660.6948110227342725No Hit
CATTCTTC27410620.6819231898694927No Hit
CAAGCACT27385610.681300989460358No Hit
CGCTGAAG27380720.6811793357218267No Hit
TCTCATTC26855830.6681210881108424No Hit
TCGTCTGG26833120.6675561072515281No Hit
TGCTTCTT26600330.6617647424677429No Hit
GATACACC26501190.6592983310898294No Hit
GCCGGTTG26455190.6581539400934202No Hit
TGCTAACT26428150.6574812372120525No Hit
CAGCTAAC26345210.6554178504893964No Hit
CCTACTCG26216580.652217784970524No Hit
AGATGGAC26182830.6513781502720715RNA PCR Primer, Index 16 (100% over 8bp)
GGTCAACT25823560.6424402078094635No Hit
AACAATAG25804350.6419623001781368No Hit
CCAGGATA25529500.6351245639745914No Hit
ATTAGTGC25357460.6308445416480205No Hit
GCTCACGT25318110.6298655897847878No Hit
AACAGAAT25201480.6269640610475875No Hit
TTGTCACA25077980.6238916200028799No Hit
TTATGCTA25034130.6228007168465202No Hit
CTACAAGC24982750.6215224818596612No Hit
CAGAGGAT24747960.6156813609455973No Hit
TATACTAT24707610.6146775310172252No Hit
GGAACCTG24576320.6114112898450823No Hit
CCACATAT24488120.6092170444997932No Hit
GGTTCAAT24423770.6076161410080771No Hit
TGTATATT24190110.6018031323076206No Hit
TACGTACC24059630.5985570423682405No Hit
ATCATCTC24023150.5976494905519577Illumina PCR Primer Index 7 (100% over 8bp)
CCATAAGA23738230.590561232232459No Hit
TCCGTATG23434470.5830042711657354No Hit
TTGGAACG23397890.5820942315429386No Hit
GGCCAATA23323880.5802530059419766No Hit
ATGGTCAA23231260.5779487995487717No Hit
CATCAGTG23175410.5765593596107399No Hit
AAGCATCC23101650.5747243535260628No Hit
GTTCTAAT23011680.5724860740054769No Hit
AGTAACCG22971210.5714792587092882No Hit
CGTTGTCT22916050.5701069846362026No Hit
GTTACCAA22847300.5683966176578734No Hit
CGCGATTA22800310.5672275973769761No Hit
ACCAGCGG22535760.5606461052443658No Hit
AGTCATGA22520000.5602540269377699No Hit
CGCAAGTG22463400.5588459284508838No Hit
GTCCTCAA22371550.556560878167836No Hit
TATCAAGA22269580.5540240618655782No Hit
GTCTGGCG22251330.5535700371767854No Hit
GGCTGAGT22213930.5526395975405743No Hit
ACACTGTG22185040.5519208702386991No Hit
TGCAATCG22082320.5493653953875868No Hit
TTCGTTCG21981110.5468474864148349No Hit
CCGACACA21801120.5423696834704065No Hit
ATATTACA21687250.5395368181929907No Hit
TAATGATT21551690.5361643476827027No Hit
GAACGCGC21479290.5343631757666151No Hit
GCGACAAG21192020.527216453994039No Hit
ATATTCGG21173050.5267445170983459No Hit
TATAGGTG21170250.5266748585159557RNA PCR Primer, Index 39 (100% over 8bp)
AGCCACGC21146700.5260889800819245No Hit
CGATGCTC21088100.5246311254647595No Hit
ATCTCAGT21080100.5244321009436449No Hit
AAGATGGC21015010.5228127876837257No Hit
CTTCGACG20983650.5220326115609563No Hit
CGGATGCG20934910.5208200546660652No Hit
CGACTCTG20866800.5191256096494252No Hit
AATTCATT20843640.5185494336607983No Hit
GACATCTA20708930.5151981095058789No Hit
GGATTGTG20675030.5143547430976555No Hit
AGAACACA20661950.514029338005633No Hit
GGCAACAC20634600.5133489229240723No Hit
TCGCAACA20571860.5117880731172305No Hit
CCGACCGC20557440.5114293314179214No Hit
GGACCGGC20503180.5100794476034611No Hit
GATTGAGG20233280.5033648578223553No Hit
ATGCAACT19835510.49346910985188297No Hit
TCTAGTGA19631970.4884054284734232No Hit
GTTCGGAC19443450.48371541563335624No Hit
CAGTGGTC19416050.48303375664853854No Hit
TATTGACC19391230.48241628307178025No Hit
AGAGCCGC19359750.48163312158119415No Hit
CGTTCATA19349720.4813835945878466No Hit
AGATGACT19127860.47586414705603425No Hit
GAACAGAC18903730.47028822631635564No Hit
GCATAACG18743730.46630773589406244No Hit
CGTCGTGG18685720.4648645593353298No Hit
AGGTCTTG18580360.46224340639224964No Hit
TACACACA18542470.4613007765041204No Hit
TTACAATT18517480.4606790736562884No Hit
GAGCTTAG18426780.458422633148151No Hit
GTCCATCC18152970.4516107701323503No Hit
ACATCCAG18056060.44919983683969755No Hit
ATTGCCAG17883410.4449046388933918No Hit
GCGTACGA17706430.4405017189250327No Hit
GATCGGCG17588230.4375611316255636No Hit
ATGTCAAC17343480.431472225182712No Hit
CACTGCTT17010420.42318633680740586Clontech Universal Primer Mix Long (100% over 8bp)
TCTCCACG16963700.42202403360409624No Hit
CTATGGAG16857610.41938471967346447No Hit
CAGTACCG15894670.39542863562819547No Hit
TACTCCGG15670270.38984599781092927No Hit
CAATCAGC15659350.3895743293396078No Hit
TAATACCA15340590.38164419729579413No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)