FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402195810
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCC47884041.1905653616829075No Hit
ACAAGTGT46272291.1504915976126155No Hit
CTATTGGA42997971.0690805058361996No Hit
AACGGCCG42030791.0450330151375768No Hit
TCGGAGCT41544891.032951835077546No Hit
GTCGACCG41374451.02871409823986No Hit
CCTTGGTT41323881.0274567504818113No Hit
GACATCTA40863081.015999644551245No Hit
TTCGACAA40241171.0005367783418728No Hit
GTCCGCGG39321980.9776824875425728No Hit
CCTGGTGC39178450.9741138277895038No Hit
CCGGCTGA37578530.9343341990559275No Hit
GCCTACAT37512640.9326959423073055No Hit
TATGCCGC37281430.9269472498980037No Hit
TATGCTTA37057840.9213880174435432No Hit
CGGTTGTT37037650.9208860231537469No Hit
TAGGTCTC35909710.8928414744052158No Hit
GCAGATCT35898890.8925724512147454No Hit
TCCGGTCT35839420.8910938182076038No Hit
ACCTATAA35737680.8885642045848265No Hit
CGGTCGGA35031490.8710058416570774No Hit
TTAGGTCA34240520.8513395502553842No Hit
TATACTAT33676360.8373125518140033No Hit
TGACGACC33456840.8318545138498584No Hit
AGTAACCG33349260.8291796973220581No Hit
CGTTGTCT33306560.8281180253966345No Hit
TACATGTC32748380.8142397107518351No Hit
TCAATAAC31673340.7875104417422946No Hit
GGCGTGTC31648830.7869010370843992No Hit
GGACGTTG30742010.7643543079178274No Hit
TGGTTCCA30380170.7553576950490857No Hit
GTCCGACT30329050.7540866723599134No Hit
TGAGCTCC30065310.7475291699334212No Hit
GCATAACG29686330.738106396483842No Hit
AGACCATC29653420.7372881383324207No Hit
CAGATAGT29147700.724714163481713No Hit
CCATGCGG29103260.7236092290469163No Hit
ATTAGTGC29095710.7234215095378542No Hit
TATGAGGT28868010.7177600880526329No Hit
CACCGGAG28642710.7121583389941333No Hit
ATCTGCTG28053870.697517709097964No Hit
AGATGACT27873130.6930238780955973No Hit
CACCACGG27741880.6897605422592543No Hit
GAAGGTGA27524910.6843659062485012No Hit
TGAGATAA27502540.6838097095044327No Hit
GGCAACAC27037220.6722402205035404No Hit
CTCTGAGA26931000.6695992183508824No Hit
CGTAGTTC26844840.6674569782315734No Hit
CCTATACC26714660.6642202463521437No Hit
AGAACGGT26705560.663993988400824No Hit
ACACGAAT26300450.6539215314053122No Hit
GCGCCTAA25999020.646426923244178No Hit
AGAAGTAG25799760.6414726200156088No Hit
CGCCGGTA25772360.640791359810536No Hit
TAGCGCTG25440650.6325438845322631No Hit
CGGCTTGA25354470.6304011471427313No Hit
TGTATATT25241020.6275803818045742No Hit
CGCGGTCG25002610.6216526721151073No Hit
GACAGTCG24851580.617897536028533No Hit
AACCGTTC24771520.6159069633271416No Hit
CTCGAACA24711600.6144171417399898No Hit
CAGCACAA24494340.6090152953110078No Hit
TGCTATAC24378050.6061239176012302No Hit
CGACTTAC24271200.6034672514365578No Hit
GATACGAG24084990.5988374170282877No Hit
TCATTGCG23964370.5958383803152997No Hit
GCTTATAC23710890.5895359775130427No Hit
CCACCAGG23538730.585255475436206No Hit
AACAGTGA23288390.57903114405891No Hit
AAGCATCC22834690.5677505690573953No Hit
AGTCTACC22550510.5606848564633232No Hit
GTGTGTGT22491520.5592181579415261No Hit
CGCCAGCC22420990.5574645345012421No Hit
GTAATTCC22379540.556433941964736No Hit
AGCAGGAA22339170.5554302020202547Illumina Paired End Adapter 2 (100% over 8bp)
CAATACAT22289500.5541952314222269No Hit
CCTAACCT21825590.5426607999720335No Hit
TCAAGGTT21711150.5398154197578537No Hit
ATAGCCTA21485250.5341987525926737No Hit
CAACAGCA21483010.5341430583277335No Hit
TGGAAGAT21055070.5235029673730316No Hit
GATCTTCC20871140.5189298217701472No Hit
CGTATGAC20756950.5160906574337509No Hit
CTCTCGAA20668090.51388128583438No Hit
TTCCTTAT20594500.5120515800500259No Hit
TGGCCGCA20469790.5089508515764996No Hit
CTGATTAC20422540.507776050675416No Hit
CTCATAGG20106590.4999204243326155No Hit
CGTATCCA20084700.49937616207389135No Hit
GATATTGT19915440.49516776417934344No Hit
CGTGCAGG19544520.4859453906295046No Hit
ATGAGACG19450940.48361866325758096No Hit
CAATCAGC19305310.47999779013113036No Hit
CAGGAATC19111390.47517625805201696No Hit
TATTGACC19009300.4726379422003426No Hit
TAAGACTG18847910.4686252201384197No Hit
CGGATGCG18682120.4645030986275068No Hit
CTGTAAGG18505190.46010399760256077No Hit
AAGCCTGT18320250.4555057398534311No Hit
TAGCCACC18080160.44953625946525894No Hit
GATCGGCG17793370.4424056531071271No Hit
CGATGGCA17703350.4401674398348407No Hit
GCGCAATC17418480.4330845714180861No Hit
ATGTGCAT17067890.4243676730496024No Hit
CAGTGGTC16388630.4074788844766931No Hit
TGGCGCGA16305880.4054214289303511No Hit
AACGTCGA16278790.4047478764137299No Hit
CTTATGTA15149710.3766749832625059No Hit
GTTATCCG14758740.36695409631442955No Hit
AGCGTGCG14225500.353695877637313No Hit
GGAATATC14146670.3517358870546165RNA PCR Primer, Index 37 (100% over 8bp)
GCCTTAGG13924410.34620972306996434No Hit
ATTAATCA13888550.3453181175606976No Hit
GACTCGGT13451530.33445226592489863No Hit
TCTATGAA13391460.33295871481107675No Hit
TTCCGGTT13064310.3248246171435749No Hit
TGCAGTGT12841870.31929397772691864No Hit
GCGTACGA12679420.31525490034319353No Hit
GCTGGAAT12580420.31279341274092337No Hit
CGAGGAGT12498940.31076753385372163No Hit
CTGGATGC11874990.29525394608163624No Hit
CTGACTGG11814100.2937400068886844No Hit
CGCGTCGG11621720.2889567646167174No Hit
ATAAGCCA11540090.2869271562028456No Hit
CTTGCGAT11442900.28451067155572807No Hit
CTTGCCGA11344910.28207429609970325No Hit
CCGGTAGG10970450.27276390572044995No Hit
CTAATGAT10934110.2718603657258389No Hit
AGTGTCTC10929540.27174673947995626No Hit
GCCATGCG10892060.2708148550826524No Hit
CGAGCCTT10815590.2689135423862322No Hit
TACTGTTG10656040.26494656918479587No Hit
ACTCCGTT10495230.26094826795933057No Hit
GTTGCCTT10404180.25868444527057605No Hit
TAACCTCT10367310.25776772761506395No Hit
CTATCCTG9920320.24665398677325853No Hit
GCGGAAGT9561630.23773569396458905No Hit
AGGCCAGA9054450.22512541838762565No Hit
CCACTACA8527140.21201464033153403No Hit
GTAGCTTC8223310.2044603597436781No Hit
ACGTGGTA8161610.2029262811067077No Hit
GGCTCTTA7948770.19763433139693823No Hit
GACGCCTC7442360.18504320072354807No Hit
GAGTATAA7415140.18436641595047945No Hit
ACCTCGCA7316660.18191785737399901No Hit
TAGGATCA7175860.1784170750063259No Hit
GTGGTACC7105260.17666171112026255No Hit
GCATCCGT6826810.16973846644498858No Hit
ACTCTTGT6774270.16843213757000602No Hit
ATCATCTC6606960.16427222352216947Illumina PCR Primer Index 7 (100% over 8bp)
GAGTGAAC6486300.16127219226873596No Hit
AATTAACA6466080.16076945207360566No Hit
GTGGACAC6441010.1601461238494752No Hit
CTGAACTA5619440.1397190090070804No Hit
TCTCCACG5611020.13950965824333178No Hit
CGCGATTA5433090.13508569370725168No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)