FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404727063
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCGC59941581.4810371106811802No Hit
GGCTACGG56105861.3862641055955283No Hit
TCTGATAT49097291.213096293489027No Hit
GTGTAGAT49026001.2113348595124709Illumina Single End PCR Primer 1 (100% over 8bp)
GCCTGGTC47690671.1783415135745445No Hit
GTATATCA47333991.1695286608496451No Hit
GTAGTGAC44152581.0909223532699617RNA PCR Primer, Index 43 (100% over 8bp)
AACAGAAT43885531.0843240794105238No Hit
GCGCTCAT43346741.0710116511284544No Hit
TGTAGCAC43124921.0655309205255692No Hit
GAGAATTC40972521.012349401502711Illumina Small RNA Adapter 2 (100% over 8bp)
GGTCTCGC40778611.0075582714368672No Hit
CATCAGTG39174270.9679182239414515No Hit
GACAAGGC39015600.9639978041201558No Hit
CACGCCGT38808900.9588906586165206No Hit
TCCGGCGA37748490.9326900385705119No Hit
GTAATCTT37441540.9251059151436088No Hit
GCACTCTC37311150.9218842378227621No Hit
TATCACAT37214480.9194957145724648No Hit
TGGCACAG37146910.9178261943901687No Hit
CATACGTT36962460.913268802091448No Hit
AAGTGGAG36172070.89373983869223No Hit
CCATTGAC36164470.8935520578222367No Hit
GTGGTCGT35811090.8848207415277292No Hit
GCCATCGC34998060.8647323888000047No Hit
CACACCAC34635990.8557863599054656No Hit
AATCAGCT34400080.8499574934528161No Hit
CCACATAT34286840.847159558489915No Hit
AACAATAG34166400.8441837258607042No Hit
ACGCCTCG33770610.8344045428956155No Hit
GAGACTGA33402120.8252998885819504TruSeq Adapter, Index 15 (100% over 8bp)
TGCGGATT32883120.8124764318021402No Hit
ATGGCGCT32849980.8116576083769323No Hit
CCTGTGCG32770340.8096898625234754No Hit
AGGCGGTT32405890.8006850285670173No Hit
GTCCATCC32188620.7953167194060359No Hit
CGAGTTCT31758220.7846823922422009No Hit
GTTCTAAT30968330.7651657828475878No Hit
AAGGTGTT30845150.7621222502731427No Hit
CTCTAGTT30481230.7531305115615656No Hit
GTAAGAGA30436030.7520137095452892No Hit
CACTTGCT30388490.7508390907874624No Hit
GTATCTAG30209450.746415368818566No Hit
TCTTAGTA29980470.7407577288697396No Hit
TGCTCGAG29879720.738268396941867No Hit
GCTGTAGC29751040.7350889703167688No Hit
AGAAGAGC29394480.7262790825529747No Hit
CGGACATC29210050.7217221844144384No Hit
TACGAGCG28983920.7161349622918595No Hit
GACGCAAG28796850.7115128350090095No Hit
TACCTGCA28608990.7068711883988841No Hit
TGTGTCGA28156290.6956858726296739No Hit
CGGAGCTG27832440.6876841838471276No Hit
CAGTACCG27774310.6862479072717704No Hit
CTTGCTTG27754450.6857572062088668No Hit
TTGTGTAC27502010.6795199163639818No Hit
ATACATGT27471630.6787692870441926No Hit
CTGTCATT27192590.6718747641543309No Hit
GAGTCCTA27146070.6707253475658977No Hit
AGCCACGC26937020.6655601382406197No Hit
GGTTCAAT26902920.6647175951265705No Hit
CTAGTCCG26765260.6613162905787696No Hit
CATTGTGA26597260.6571653450315479No Hit
GTGCCACG26531600.655543017146842No Hit
ACCGAACT26529620.6554940952886069No Hit
TCTCTCAG26517340.6551906809355124ABI Solid3 Adapter A (100% over 8bp)
TAGCATGG26117570.6453131600937692No Hit
CGATCTAA26087300.644565248654993No Hit
CAATCGTA26048520.6436070720578426No Hit
AATGGTCT25856330.6388584397678393No Hit
TTAAGTGG25596600.632441028535816No Hit
AAGGCCTG25063820.6192770953890968No Hit
ATCTAACC25051920.6189830700795019No Hit
AATTATTG24627440.6084950143301882No Hit
TCGTGTCA24575800.6072190927346017No Hit
TTAGCAGT24519820.6058359383790454No Hit
TCCGTATG24492450.6051596801669772No Hit
CTTCGACG24336030.6012948533664031No Hit
CATGACTA23846200.5891921292152386No Hit
ATGTCAAC23749020.5867910048802444No Hit
GTGTTAAG23394780.5780384396978168No Hit
GGTTAATG23311150.5759721088876134No Hit
AGGACCAG22206720.5486838422761959No Hit
AACTTGAA21563980.5328030164367832No Hit
TGAATTGA21490510.53098771900015No Hit
CCGACACA21274750.5256567189330752No Hit
AACGGATA20442830.5051016319113802No Hit
ATTCTGGC20423480.5046235319331734No Hit
GAGAAGCT19716010.4871433566576199No Hit
AGTGGCAA19484880.4814325944889927No Hit
AATGCGAA19374700.4787102660342731No Hit
GCTAGTCT19225430.4750221014995481No Hit
CGACCTCG19150960.4731820960537052No Hit
GACCACTT19118940.4723909455988122ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CGCAAGTG18726650.46269824066595716No Hit
TATAGGTG18283260.4517429564624889RNA PCR Primer, Index 39 (100% over 8bp)
TTATGCTA18097840.44716159739483496No Hit
AGTTACAT16975450.41942957493801203No Hit
CTTAACAC16518160.40813084940652955No Hit
TGGCTGTG15625320.3860705504637825No Hit
ATCGGCGC15136850.3740014292051431No Hit
CGATGCTC15056510.3720163877452396No Hit
CCAGCGTG14837580.36660706328897014No Hit
CCGAAGGT14474020.3576242194607085No Hit
TCGTGAAG13952340.3447345452162165No Hit
ATAGGTAT13927330.34411659790588306No Hit
GGACCGGC13792190.34077755754129047No Hit
TCGGCAGC13182780.32572024964883556No Hit
GTTACCAA12830600.3170185829653798No Hit
TGTCTTAA12777960.31571795336058367No Hit
CGAGAGGA12364570.305503909433405No Hit
CATCTCCG12254610.3027870167407115No Hit
GCACCACT12194360.30129835918582987No Hit
AGCAAGCT12164600.30056304883175056No Hit
GATGGCGT11548980.2853523042021037No Hit
TACTGCAC11272220.27851411557324No Hit
TGTCCTGC11236750.27763772248657365No Hit
ACAGGCTG11193680.27657354852991384No Hit
ATCGCGTG10962070.270850926516866No Hit
TGAGCGTA10747740.26555525890296094No Hit
CGGCACTC9811310.24241793783876517No Hit
GGCCGTCA9790870.24191290613051986No Hit
CGTAATGT9162380.22638416942234477No Hit
GCATGTAT8934850.2207623560868723No Hit
TCATTCAA8620370.2129921813506205No Hit
TAATAGGC8408300.20775235383752927No Hit
TCCTTACA8241260.20362512797914875No Hit
TAGATCCG8032340.19846313069506794No Hit
ACTACATA7129910.17616588194400037No Hit
CACTGCTT6871530.1697818265244101Clontech Universal Primer Mix Long (100% over 8bp)
CCTACTCG6762440.167086429799729No Hit
GTACTATA6154890.15207507880440405No Hit
ATCGCTAA5610350.13862057946937933No Hit
TTACAGCG5375070.13280727906253206No Hit
AGATGGAC5237190.12940053875270505RNA PCR Primer, Index 16 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)