FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399024297
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTGTA43480471.0896697350738016No Hit
CTGTGTTG41882481.049622299065162No Hit
GCTGCATG41433611.0383731093948898No Hit
GTCACAAT40000831.0024660227645235No Hit
TCTCTGCC38410590.9626128105176512No Hit
TCCGCGAT38367360.9615294178439464No Hit
CCGTTACT38294440.9597019602041928No Hit
GCGGCTCC38196860.95725649508506No Hit
GCGTCGCT38102440.9548902231384672No Hit
CGACGCCT37142010.9308207615237023No Hit
TCTGTGTT36334510.9105838986040492No Hit
AGACTCAA36052630.9035196671244308No Hit
CTCGGCCT35985040.9018257853105122No Hit
GAGTTCCT34194470.8569520767804272RNA PCR Primer, Index 1 (100% over 8bp)
TGTTGCGT33293630.834376007935176No Hit
TTCTATGA33165760.8311714411716637No Hit
ACTTGTCC32967530.8262035732626075No Hit
CGTACACT32325270.8101078115551444No Hit
AGTGCTCA31686860.7941085352003014No Hit
GATTGAGG31407150.787098686373978No Hit
CCATAAGA30993300.7767271374955896No Hit
TACAGCCT30755850.7707763720463368No Hit
AAGATGGC30647460.7680599961059514No Hit
GTCTCCAC30641380.7679076244322035No Hit
GCAGTGGT30546650.7655335835351399Clontech Universal Primer Mix Long (100% over 8bp)
GAACGCGC30507230.7645456737688332No Hit
CCTCCATC30119080.7548181959455968No Hit
GCGATCCA29952630.7506467707654404No Hit
GCCACTGT29698940.7442890125560448No Hit
GCACACCA29458290.73825805148903No Hit
TTATCGTC29453010.7381257287197226No Hit
TACGTACC29399960.7367962357440103No Hit
GGTCGTAT29225590.7324263264098928No Hit
CCGCGTAC28501680.7142843233929688No Hit
TTGACCGA28482610.7138064076333678No Hit
TTAGGATC28407950.7119353436264559No Hit
AAGAACGT28085680.7038588930838966No Hit
TGTCGGCT27875240.6985850287708169No Hit
TTATGACG27873310.6985366607888541No Hit
TCAACTTA27715610.6945845205010159No Hit
GTGATGTC27477250.6886109494229621No Hit
CACAGGCA27402610.6867403866386613No Hit
GCAAGCAC27327830.6848663152960833No Hit
TTATACGT27095630.6790471207822214No Hit
AGCGTTAT27002370.6767099197470675No Hit
AGGATCTT26786850.6713087448907904No Hit
CTCCAATG26517170.6645502592038901No Hit
TATACGGA26325810.6597545612617168No Hit
TTGCTCTA26160790.6556189734982479No Hit
CAAGAGAG26143660.6551896763319152No Hit
GTCGAAGC26083190.6536742297675172No Hit
CTAGCGGC26068580.6533080866501721No Hit
GAAGGCAG25365310.6356833453678135No Hit
ACACTGTG25190780.6313094262528078No Hit
ACGATAGA24802770.6215854569878485No Hit
CGACTCTG24737810.6199574859472781No Hit
AACGCGCC24491780.6137916959979006No Hit
ACGCATTA24183740.6060718653430771No Hit
TGCTAACT24141660.6050172929695056No Hit
TGATTCGC23657280.5928781825533797No Hit
CGTGTGGT23655540.5928345761862216No Hit
ATGAATTG23545070.5900660730942908No Hit
GCTGACAA23539740.5899324972684558No Hit
CTTCGCAA23220070.5819212056653281No Hit
AGTCTGAT23075040.5782865899015668No Hit
AATATTCG23074150.5782642854953768No Hit
ATTACGAC23038120.5773613329616367No Hit
CGAATCGT23005620.5765468462187404No Hit
CCTTGTAG22980930.5759280869054447No Hit
ATCTCAGT22797310.5713263621137337No Hit
GTTCACTG22592180.566185572403878No Hit
CATATCTC22298280.5588201061350406No Hit
CACATTAT22297710.5588058212906268No Hit
CGGTATAT22141540.5548920245325312No Hit
TTGCGTCG21995740.5512381116982458No Hit
AGAACACA21281750.5333447150963841No Hit
TGAGTAAG21245070.5324254728277862No Hit
CACGATAC21199610.5312861938329535No Hit
CTCAATTC21111930.5290888339062721No Hit
CTTCTAGA21067770.5279821343811553No Hit
AGCTCACG20832730.5220917662565294No Hit
TCGGATTG20680670.518280970744997No Hit
CATTCCAG20440570.5122637932997849No Hit
AGGTAGCC20385080.5108731511655291No Hit
ATACGTTA20344040.5098446423677303No Hit
TCCACAGG20334710.5096108220196927No Hit
GACTAAGA20092760.503547281482962No Hit
GACGATGT20080310.5032352704076063No Hit
GATCGATT19559300.49017817077940995RNA PCR Primer, Index 42 (100% over 8bp)
GTAGAATG19439820.4871838669012178No Hit
ACGGACGG19101870.47871445783162425No Hit
ACGATTCT18143570.4546983764249324No Hit
ACTTAAGG18105660.4537483089657571No Hit
TAAGTCCT18013270.45143291111418204No Hit
TCTAGTGA17604280.4411831593302701No Hit
TGTGGAGC17170440.43031063845217427No Hit
ACCTAGGT16210710.40625871962879495No Hit
CAAGACGC16119380.4039698865756037No Hit
AAGTGAGA16067640.40267322368091285No Hit
ATAGTTGC15753210.39479325240187063No Hit
GGCCTAAG15440760.3869629021613188No Hit
TCACGCCG14405500.36101811614744855No Hit
AGAGCCGC14249100.3570985553293262No Hit
GCGCGAGG14222900.3564419537088991No Hit
TTAGAGAT14007910.35105406125181393No Hit
TACTCAAT13691010.34311218898031165No Hit
GAGGTATA13501770.33836962063490583No Hit
GACTTATC13483350.3379079946101628No Hit
GGTAGCGA13453090.3371496447997No Hit
AATCTGTA13342350.33437437520252056No Hit
TTCACGGT13308560.333527559601214No Hit
ATTGACCT13260360.3323196131086724No Hit
AAGCAGGA12722110.31883045958978284No Hit
TAACGGTC12236200.30665300564391446No Hit
CGGAGAGA12232230.3065535129556284No Hit
GGTAATTA11955910.29962862136187157No Hit
AGTGGATG11830720.2964912184282352No Hit
TGACGGAA11189090.28041124523301897No Hit
TGTGAACA10853750.2720072457141626No Hit
TTCTTGCC10815070.2710378811844633No Hit
TCTGACCG10387860.26033151560191836No Hit
TCACCGAG10338080.2590839725231068No Hit
GGTGTACG10201020.2556490939698341No Hit
GCGAGATA10157810.25456620251874035No Hit
TCGCACGC10041920.25166186809922503No Hit
TCGCAACA9872150.2474072399656405No Hit
TAATACCA9749950.24434476981235057No Hit
GGTTGGCC9521430.23861780025891507No Hit
TTAATACT9408180.2357796272240535No Hit
TCCAACCA8789550.22027606003150227No Hit
GACCTGTG8705700.2181746842348299No Hit
AATCGCGG8684950.21765466577590387No Hit
GATACACC8466460.21217905936189146No Hit
GAACAAGT8216060.20590375227200766No Hit
CGCACCGA7369410.1846857460912963No Hit
TTAACCAG6916910.17334558451712528No Hit
TGATCCAT6268400.15709319074372055No Hit
AACCAACG5900000.14786067024885957No Hit
TCCGCGGT4016540.10065903330192447No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)