FastQCFastQC Report
Sat 16 Sep 2023
EGAF00001836202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396689624
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGATA43761731.1031730439211085No Hit
GTTAAGCC42811261.079213002052204No Hit
TACAAGAA42007991.05896366979339No Hit
CAGAGTGC41961311.05778693117519No Hit
ACTGGTTA41028411.03426980484874No Hit
TGCTTCTT38126480.9611161395035631No Hit
GCGGAGTA35420290.8928968104293044No Hit
CATTCTTC34723830.875340011414062No Hit
TCGTCGAC34212250.8624437830014934No Hit
CTGCCTTA33548410.8457092893359873No Hit
CCTGGCCA33218240.8373861575971041ABI Solid3 GAPDH Forward Primer (100% over 8bp)
CTATCGCT33210000.8371784385265393No Hit
CTCGTATC32985270.831513304214884No Hit
TGCGCCTG32981920.8314288553209044No Hit
TCATCATG32884460.8289720227217237No Hit
GGAACCTG32840930.8278746912724896No Hit
TCCTCCTA32667640.8235062886343606No Hit
TGCCGTAG32101410.8092324088617956No Hit
TCTCGAGT31904430.8042668138957928No Hit
CATGTATT31825860.8022861722241568No Hit
GATGGTAC31134020.7848458370567312No Hit
GAACAGAC31050510.7827406647772567No Hit
AGGTCTTG31011320.7817527387608202No Hit
CGTTCATA30980580.780977825626213No Hit
GCCGGTTG30482090.7684115781157916No Hit
ACATACTC30428780.7670677063133872No Hit
ACTGTCAT30402500.7664052236465857No Hit
TTGACTCT30202830.7613718174791484No Hit
TCTCATTC29803740.7513113073005409No Hit
TGCTCAGC29738570.7496684612048234No Hit
GGATATGC29675240.7480719989792323No Hit
CGTGTTAG29674460.7480523362516787No Hit
TTCAGTAA29611090.7464548656810848No Hit
CTAGGCAC29465010.742772389731071No Hit
GCTTCTCA29377880.7405759622288483No Hit
ACAGTGCC29238610.7370651570155513No Hit
GCAGTTAA29218000.7365456072528885No Hit
GAGCGTTA29096060.7334716675120295No Hit
ACTGAGGC28723010.7240675899302069No Hit
CTGCGCTT28714470.7238523082721215No Hit
TAGGTGAA28702240.7235440067875332No Hit
TCGTTCTG28612370.7212785076526227No Hit
TCAATCGG28521830.7189961187389161No Hit
TCCAGATC28340600.7144275596177428No Hit
GCTACCTC28190890.7106535763587303No Hit
ACCGACAC27894410.7031797231984066No Hit
TTGATGAG27829070.7015325916364276No Hit
GTCATACA27801480.7008370856707863No Hit
ATACTTAG27511570.693528853177163No Hit
AGAGATCG27344670.6893215336532221No Hit
AGAATGTA27176150.6850733761566701No Hit
GCGACAAG27004080.6807357280411246No Hit
CTTCTGCT26653970.6719099363183747Illumina Single End Adapter 1 (100% over 8bp)
ATGCGGCA26437460.666452016904783No Hit
ATACCGAA26273440.6623172982210395No Hit
ACTATTAC26219740.6609635950548584No Hit
TTGTGGCT25736860.648790854181757No Hit
ATGGAGAG25618910.6458174968549215No Hit
ACCAGGCC25563980.6444327870799061No Hit
GTAGCACA25469520.6420515803559308No Hit
CAACTCGA25331810.6385801005977408No Hit
TATCAAGA25251400.6365530750559787No Hit
ATTAGGCT25165890.6343974855263671No Hit
ATACAGTC25120570.6332550306382604No Hit
AACACGTA24655430.6215294907738751No Hit
CTACAAGC24442470.6161610619792768No Hit
AGCAACTA24323960.6131735878224029No Hit
CCAAGACG24304920.6126936156010977No Hit
ATGTAATA24211060.6103275340521637No Hit
GCCAACTG24156310.6089473618296606No Hit
AATGTAGG24114260.6078873391455281No Hit
CAGCTAAC24016760.6054294982013444No Hit
GAGCTTAG24007290.6051907725219453No Hit
GCATCGAA22969380.5790264884770467No Hit
ACGCTATT22491470.5669790344705361No Hit
TAACTATG22265420.5612806247737904No Hit
TTGAAGGC22248070.560843255128851No Hit
CACACATG22143200.5581996266179122ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
AGTCATGA22134640.5579838407873254No Hit
AATTCATT22026590.5552600488486686No Hit
ACCATAAG21929930.5528233831495426No Hit
GCACGTCC21710340.5472878211707398No Hit
ATTCCACA21445740.5406176189776015No Hit
CAGGCGCA21348440.5381648197584317No Hit
CGTCGTGG21308710.5371632810844581No Hit
GGAAGAAT21187890.534117575003676No Hit
GTGAGTCA20750670.5230958599512046No Hit
TTGCAGAA20615510.5196886621869394No Hit
ATATTCGG19909980.5019032209423254No Hit
GCAGCTGG19404700.48916580686768857No Hit
GGAGTGCA19271000.4857954136960235No Hit
ACGACGAT19267820.4857152502683055No Hit
TTACCTAC19134070.482343596665387No Hit
AAGCTCCA19079350.4809641807016359No Hit
CGTGATCC18569210.46810425270916595No Hit
ATTGCCAG18472510.46566657866503713No Hit
GTGTCGGC18408110.4640431432106225No Hit
TCGATTGC17944910.45236650807887024No Hit
ACTCATAG17867050.4504037645310329No Hit
CCGAATAG17707850.4463905514201198No Hit
CTTACAAG17488090.4408507039750553No Hit
TGCAATCG16639720.4194644627256497No Hit
TCCGACTT16481070.4154651143585243No Hit
TAATTAGA16188260.40808377685220226No Hit
CGCCTCAT16091800.40565215287808987No Hit
GGCTGAGT15940780.4018451463202375No Hit
GTTGCAAC15529140.3914682678970197No Hit
ATTCGATC15358260.3871606180453059No Hit
CAGGCTAG15071500.37993179272064853No Hit
TTCCGAGC14417310.3634405622870539No Hit
TGGAGTTA13788630.347592403878958No Hit
ATGGTCAA13775690.347266204270571No Hit
TGTGAGTC13394670.33766121394695214No Hit
TTAAGGAC12989340.3274434019479168No Hit
GTCTTGTA12594090.3174796928895725No Hit
TACTCCGG12529510.3158517198826456No Hit
TAGGCATT12492830.31492706751513116No Hit
TTACAATT12288770.3097829954836429No Hit
ATAATATG12183490.307129031436426No Hit
GGCGGAAC12069390.3042527273161044No Hit
AGCTTCAC11902280.3000401139808991No Hit
ACACTAGC11525270.2905362102438051No Hit
GGTCCGTG10507900.2648897113578146No Hit
CCATCTCC10490620.2644541063166301No Hit
ATAACTGA10432650.2629927623214062No Hit
AGCGAAGA10274860.25901509337183976No Hit
ATATACAA9905000.2496914313039859No Hit
GTGCGGTG9886030.2492132236864355No Hit
TATCCGAC9831610.24784137031020506No Hit
GGACACAT9797340.24697747072910584No Hit
ACAGCGGA9288400.2341477930867181No Hit
GGATTGTG9227050.23260124393876258No Hit
GGCCGCTC8353210.210572939008861No Hit
GTTCAGGA7443140.18763132559272586No Hit
TACACACA7332760.18484879755765932No Hit
GGCACTCC7238230.18246582622992932No Hit
TTCGCATA6873460.1732704760636744No Hit
CGCTTACC6007390.15143804215055548No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)