FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410813438
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCGCA178197914.3376845428313375No Hit
GCGTACGA167889724.0867631014543395No Hit
TCGGCAGC159656793.8863575343900996No Hit
CAGGCTAG153297573.7315617216981107No Hit
AGAGCCGC94540532.3013008157732173No Hit
ATAGGTAT77853551.8951071897506915No Hit
GACTCGGT76970101.8736022943825905No Hit
ATGTGCAT73370631.7859841770804001No Hit
CGAGAGGA71028991.7289840942350088No Hit
CTTGCGAT67595041.6453950564294832No Hit
CGAGGAGT67264301.6373442000210323No Hit
ATTAGTGC62185811.5137238524315264No Hit
CGAGCCTT61235141.490582691211771No Hit
TGAGCGTA57433881.3980526119011716No Hit
GTCCGCGG56853851.383933550878635No Hit
TATGCTTA55826081.358915625345245No Hit
GTCGACCG54360001.3232283798856648No Hit
AACAGAAT52953641.2889948356557899No Hit
GGAATATC51880481.2628720290303648RNA PCR Primer, Index 37 (100% over 8bp)
GGTCTCGC51846761.2620512184900825No Hit
GACGCAAG51831721.2616851155682012No Hit
CCAGCGTG50415521.227212046554329No Hit
ATTCTGGC50137101.220434760948594No Hit
TGACGACC49091371.19497965400051No Hit
GAGTATAA48954171.1916399385163248No Hit
GTAGTGAC47122511.1470537631244673RNA PCR Primer, Index 43 (100% over 8bp)
TGCGCCTG46335691.1279010303455554No Hit
ATTCGATC45456001.1064876607079246No Hit
ATCGCTAA44744681.0891727451232986No Hit
ACTACATA44324981.0789564288790379No Hit
CCTACTCG43505581.05901063538238No Hit
TCTGACCG43455811.057799136551127No Hit
ACGATAGA40710960.9909841362102668No Hit
GAGAATTC40511240.9861225620375154Illumina Small RNA Adapter 2 (100% over 8bp)
AGGCCAGA40443990.9844855659273736No Hit
GCGGAAGT39594610.963810000781912No Hit
ATATACAA38989240.9490741147566842No Hit
TTAATACT35953200.8751709821137836No Hit
TAATAGGC35518080.864579313006796No Hit
TCTCCACG34018240.8280702833289499No Hit
GTTACCAA32488680.7908378109091942No Hit
ATCTGTCT30234070.7359562079368981No Hit
CGGTATAT28892520.7033002654601576No Hit
TGCTCGAG28738640.699554526256758No Hit
GTGGTCGT26853640.6536699512735997No Hit
CGTTGTCT26359090.6416316401022889No Hit
TATATAGC26350510.6414227861747794No Hit
CTTGCTTG24277990.5909736088039068No Hit
ATAGCCTA23337560.5680817091479855No Hit
TCTCGAGT22532880.5484942291493395No Hit
GTCCGACT22015520.5359006781078082No Hit
ACCTGAAC21710870.5284849031642437No Hit
CCTATACC20534060.4998390534634848No Hit
AATGGTCT20524800.49961364701025185No Hit
GTAAGAGA20512850.4993227607126133No Hit
AGCAGGAA20361410.495636415866221Illumina Paired End Adapter 2 (100% over 8bp)
TTGCGTCG20184820.49133787098756004No Hit
CTGATTAC20002120.48689059679688473No Hit
AATTGCTA19737010.48043730254023487No Hit
TCTACCGT19591000.47688313448013353No Hit
GCGCAATC19426810.47288642977642814No Hit
TAGCGCTG19247130.4685126682735242No Hit
GACGATGT19231050.4681212497240657No Hit
GCAACAGC18592350.452574046518897No Hit
GCTACCTC18477110.4497688802477781No Hit
GTCCTCAA17908960.43593900158640864No Hit
AAGGTGTT17197060.41860996767101855No Hit
TATAGGTG16866610.41056617042794985RNA PCR Primer, Index 39 (100% over 8bp)
ATGCTAGG16799000.40892041121595446No Hit
CAATACAT16754730.407842793107464No Hit
CACGGCAA16662130.4055887285751349No Hit
CCATGCGG16266230.395951750731192No Hit
AACGCTGG15727420.3828360648708867No Hit
CAATCGTA15373200.3742136594859879No Hit
AACACGTA14500310.3529658151055906No Hit
CGCTGAAG14434430.3513621674663914No Hit
ATCTAACC14094680.343091990092106No Hit
AATTATTG13343960.3248180016935084No Hit
CGATGGCA13023480.31701689368788366No Hit
CTGTCATT12633980.3075357043213372No Hit
AGGACCAG12170220.2962468817780007No Hit
TGGCGCGA12064360.29367004299406585No Hit
GATCGGCG9132790.22230991382516557No Hit
TCGGCCGC8291850.2018397947342706No Hit
GCGTACGG7978260.19420640276134296No Hit
TCGGCGGC7396520.18004571700500216No Hit
AAGCCTGT7135680.17369636287311518No Hit
AACGTCGA6652450.16193360257119926No Hit
GCCTGTGA6318140.15379584540270078No Hit
CAGGCGCG6150770.149721733299289No Hit
AACTTGAA5319840.12949527712382183No Hit
CGGGCGCA4981470.12125869163997502No Hit
CAGGCGCC4824170.11742970296896667No Hit
CTTGCGGT4706540.11456635943832003No Hit
CAGGCTGG4420130.10759458165533523No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)