FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411171732
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGT65988091.6048790533100168No Hit
TCGATTGC61605371.4982880681106745No Hit
TCTGATAT58530131.4234959615365776No Hit
ACTCCGTT57853531.407040550151439No Hit
GGACCGGC52980761.2885311872558398No Hit
CTTAACAC52664821.2808472932667463No Hit
TCTCTGCC51037201.2412623735524697No Hit
TACTGCAC50089981.2182252840280372No Hit
AGTGGATG49284141.198626660453399No Hit
GACAAGGC48478141.179024145560668No Hit
GGTAGCGA48006511.1675537558598508No Hit
AAGATGGC47103961.1456030737054657No Hit
ATAGTTGC46569651.1326082601417744No Hit
TTATGCTA46290891.1258286111945068No Hit
ATGGAGAG45361531.1032258900521887No Hit
GAGGTATA43087791.0479268550494616No Hit
TGTGGAGC43079661.0477291274488685No Hit
TTAAGGAC42080011.0234169016268853No Hit
TCCAACCA41339671.0054112863965075No Hit
AAGAACGT41099260.9995643377546197No Hit
GCATGTAT40935600.9955840057603961No Hit
TCGCACGC40904900.9948373591013304No Hit
TGTCTTAA39136260.9518227289029685No Hit
AATCAGCT39082930.9505257039411454No Hit
TACAAGAA38897240.9460095860870124No Hit
GGCCTAAG38830420.944384474368486No Hit
GGAGTGCA38545370.9374518479786933No Hit
TTGACTCT37945140.9228538113607478No Hit
GTATATCA37554580.9133551038961015No Hit
AGGTTATC37550590.9132580641511611No Hit
AGTAACCG37244420.9058117837731121No Hit
TCGCAACA36979040.8993575463013591No Hit
AGCGAAGA36816210.8953974005197419No Hit
TTCCGAGC36148200.8791509042747132No Hit
GAACAAGT36131870.8787537466218616No Hit
GTGCGGTG36084380.8775987547704277No Hit
TCATTCAA36084060.877590972134242No Hit
ATCGCGTG35683920.8678592719987862No Hit
GATACACC34870180.8480685145933136No Hit
GGCACTCC34767660.8455751525253199No Hit
AATATTCG34629650.8422186474628562No Hit
TAGATCCG34575830.8409097053393738No Hit
TCTCATTC33705740.8197484743430757No Hit
AGAATGTA33650640.8184084016748505No Hit
AACAATAG33535670.8156122464177571No Hit
TAACGGTC33228170.8081336194580614No Hit
CCGTTACT33028700.8032823618331816No Hit
GGCGGAAC32945840.8012671454758471No Hit
TTCGTTCG32427910.7886707056019114No Hit
CGCGATTA32091770.7804955327035955No Hit
CACAGGCA30679620.7461510024234838No Hit
TAATACCA30622840.7447700709152836No Hit
GATTGAGG30328250.7376054246842046No Hit
CTTACAAG29851830.7260185386479827No Hit
ATAACTGA29542030.7184839739907023No Hit
TCCACAGG29184800.7097958767262726No Hit
GCGCTGGC28867990.7020908236950492No Hit
GGTCAACT28692570.6978244798210009No Hit
AGAACACA28647160.696720075104774No Hit
TTCGCATA28417710.6911396817522465No Hit
TTCTTGCC26371560.6413758035292173No Hit
TACGTACC25284780.6149445117982965No Hit
CTGCTCAG24770950.6024477869504901No Hit
ACCAGCGG24674190.6000945123338391No Hit
ACTCATAG23227730.564915537530192No Hit
TAGTCGTG23102740.5618756884775338No Hit
GAACGCGC22996360.5592884483605502No Hit
TGTACGCC22967930.5585970097769271No Hit
CGGCCAAG22463190.5463213604382706No Hit
TATACTAT21957750.534028686582958No Hit
GTTAAGCC21692390.5275749355259665No Hit
ATATTACA21665210.5269138978649437No Hit
GGCCAATA21288650.5177556807334217No Hit
CTCAGAAC20421690.4966705736473148No Hit
CACTCTCG20394050.495998348446775No Hit
ACATTCCT20151440.49009789418111066TruSeq Adapter, Index 27 (100% over 8bp)
AGCGTTAT19625460.47730567236562843No Hit
ACTGAGGC19253770.4682658972285575No Hit
CTGTAGCA19242590.4679939913768197No Hit
TTGTCACA18837740.45814774056500557No Hit
GCGGAGTA18726970.4554537324078495No Hit
AGGATCTT18685730.4544507451694174No Hit
GAACAGAC18615110.4527332146461859No Hit
ACGAGTTC18606400.45252138101750633No Hit
CGAAGCAA18136290.4410879588385711No Hit
GCCATCGC17986250.43743887529700115No Hit
CATAATAA17618210.4284878708539234No Hit
ACATCCAG17327220.4214107792799336No Hit
TTGCTCTA17054210.4147709745766277No Hit
TCGTTCTG16689510.40590120139873814No Hit
GTAGCACA16550390.40251770031700523No Hit
ATCTCAGT16256200.39536278238115846No Hit
TTATACGT16019350.38960241556683667No Hit
GTCGAAGC15833100.3850726780993787No Hit
GCAAGCAC15792960.38409644367283496No Hit
TTACCTAC15626470.38004728398984394No Hit
GCACGTCC15467820.3761887988934025No Hit
TTATGACG14822630.3604973018913664No Hit
ACGACGAT14674410.35689248209310265No Hit
ACTTAAGG13913230.3383800226811312No Hit
CGGAGCTG13635710.33163053144908317No Hit
GCCTGGTC12982340.3157400908095501No Hit
ATTCCACA12981540.31572063421908586No Hit
ACCGACAC12793130.3111383639573744No Hit
ATACTTAG12673500.3082288740608267No Hit
GCTGTAGC12664860.30801874288381287No Hit
TGTTGCGT12472200.30333310948526004No Hit
GGAACCTG12295030.2990242043195713No Hit
CGAGTTCT12176920.29615168194490565No Hit
TATACGGA11969770.29111364105156917No Hit
TGAATTGA11933370.29022836618544584No Hit
CAGTACCG11568680.28135883621493707No Hit
GCAGTGGT11380300.276777295575368Clontech Universal Primer Mix Long (100% over 8bp)
AGTCATGA11049640.26873540032173227No Hit
CTTCGCAA10926360.2657371397311914No Hit
CACACATG10905970.26524123988173387ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CGCCAGCC10800380.2626732131478338No Hit
GCCAACTG10751000.2614722551014281No Hit
ATACATGT10715060.26059816777482164No Hit
GAGACTGA10572410.2571288144876652TruSeq Adapter, Index 15 (100% over 8bp)
TGTGTCGA9961430.24226932993535655No Hit
CGAATCGT9887050.24046035343694297No Hit
TAGCATGG9769020.23758977672132384No Hit
GTTCTAAT9758300.23732905840910287No Hit
CACATTAT9560630.23252157811276772No Hit
GACAGTCG9043020.21993292087501776No Hit
ATTAGGCT8982420.21845908414735085No Hit
CATGACTA8314160.20220650771780196No Hit
CTTCTAGA8229140.20013875856621388No Hit
CATTCCAG8066600.19618566579864008No Hit
CGACCTCG7077000.17211786339436388No Hit
AATTCATT6766930.16457673213780172No Hit
GTGTTAAG5632930.1369970151547286No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)