FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410631272
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCGG66482101.6190218459542944No Hit
TAAGTCCT54702851.3321647358606434No Hit
TTCACGGT53773391.3095298304509064No Hit
GAGTTCCT52294451.2735135769201718RNA PCR Primer, Index 1 (100% over 8bp)
CACCACGG52134051.2696073960971974No Hit
TCGTGAAG49537751.2063803557562465No Hit
TGGAGTTA48871881.1901645912637653No Hit
GATGGCGT48017941.1693688054035982No Hit
CGTAATGT47703381.1617084049068722No Hit
CATCTCCG47677521.1610786428365347No Hit
TGTGAGTC47577441.1586414197893822No Hit
CATACGTT45063151.0974115483342923No Hit
GCCTTAGG44949371.094640692635801No Hit
ACCTAGGT44938421.0943740300422127No Hit
CACTTGCT44681801.088124627780419No Hit
TATCACAT44009751.0717583633036112No Hit
TACTCAAT43600921.0618022292271982No Hit
TCGGAGCT43547701.0605061759641141No Hit
AACGGCCG41742891.016554092353687No Hit
GGTGTACG41107651.001084252540805No Hit
GACCTGTG39494210.9617925543673643No Hit
ATAAGCCA38981790.9493137190973608No Hit
TTCTATGA38832450.9456768796702849No Hit
TGCTATAC38272210.9320334959778709No Hit
GGTTGGCC38259030.9317125267556339No Hit
CGCAAGTG38228010.9309571045042084No Hit
TGTAGCAC38145730.9289533603763134No Hit
GTAGCTTC38029720.9261281980491735No Hit
CGATGCTC38001100.9254312223936029No Hit
CTGGATGC37491470.9130203312912807No Hit
GTGGACAC36994260.9009119013225082No Hit
CTTCGACG36563480.890421224421505No Hit
ACGTGGTA36277940.8834675406796588No Hit
TGCTTCTT35984660.8763253666661802No Hit
AGCAAGCT35753300.8706911148257603No Hit
TCGGATTG35602490.8670184768587229No Hit
GCGATCCA35123770.8553603292055165No Hit
CCTTGGTT34898890.849883883173905No Hit
GCACCACT34347080.8364457931494316No Hit
GGTCGTAT33903160.8256351211361224No Hit
CCGACACA32993200.8034750943177069No Hit
CACACCAC32959110.8026449091290836No Hit
CAGATAGT32501430.7914991432995391No Hit
CCTGGTGC32205500.7842924345031375No Hit
CTCTGAGA31848980.7756101926888802No Hit
CCACTACA31475390.7665122494616046No Hit
ACAGCGGA31426970.7653330893902304No Hit
TACATGTC31133680.7581906718492691No Hit
CTAGTCCG30889070.7522337460942332No Hit
TCTATGAA30668780.7468690791771944No Hit
CAAGACGC30264100.7370140090061139No Hit
TGAGATAA30080130.732533833906347No Hit
CTTCCTGT29362560.7150590323281565No Hit
CGCGCGCT29051060.7074731512411456No Hit
TACTCCGG28964320.7053607938559535No Hit
GCGCCTAA28705630.699060981405235No Hit
GCCATGCG28647900.69765509724744No Hit
AACCAACG28277170.6886268028802249No Hit
TTCCGGTT27940300.6804230925695304No Hit
TGGCTGTG27915260.6798132997527767No Hit
AAGCAGGA27850370.6782330499173478No Hit
AGACTCAA27605280.6722644348431408No Hit
GAGTGAAC27449840.6684790436515999No Hit
TAACCTCT26943180.6561404802116483No Hit
AGAGATCG26699130.6501971919956452No Hit
GCTGCATG26198890.6380149731996058No Hit
CATTCTTC26193100.6378739707871056No Hit
TGAGCTCC25888320.6304517401684887No Hit
CAGAGGAT25524240.6215853915772883No Hit
CTGTTCGC25319180.6165916170164458No Hit
GTTATCCG25187340.6133809506841457No Hit
CTCCAATG24295260.5916563510048499No Hit
CAACAGCA23969700.583728070277122No Hit
ATGCAACT23596940.5746503398309127No Hit
TATGGTGG23401990.5699027715551094No Hit
AAGACACT23203310.5650643675282481No Hit
GCGACAAG22955060.5590187977694987No Hit
GGAGAACC22363730.54461828713328No Hit
AGTTCCGG22341330.5440727855719669No Hit
GGCCTGCT21936700.5342189330383001No Hit
CTCTATAG21629670.5267419087360692No Hit
CAGGAATC21099820.513838605063669No Hit
CTCTAGTT20908650.5091830901763371No Hit
GGTGAGAT20836940.5074367545976869No Hit
GTGGCGAA20832570.5073303330877342No Hit
TAGCTGGT20440460.49778137696244434No Hit
TCAACTTA20235070.49277956599467176No Hit
CCTAACCT20092070.48929712299164585No Hit
TCCTGGAA20028950.4877599775206599No Hit
GGTTCAAT19716990.4801628941694436No Hit
TGGCCGCA19366820.4716352923067194No Hit
CATTGTGA19314450.4703599388796672No Hit
TGCTGCCG19006880.4628697640933689No Hit
TGCGGATT18589160.4526971340848098No Hit
CCGGCTGA18512250.4508241642151405No Hit
TAAGCAAC18430190.4488257776918656No Hit
CCACATAT18366960.44728595341856964No Hit
GTGCCACG17454800.42507235055395387No Hit
CGTTCGCG16925340.41217854445338004No Hit
CCGCTGTA16853020.4104173536982833No Hit
CGACTTAC16192210.39432481411206305No Hit
CATCCTCA15860060.38623604877321666No Hit
TAAGACTG15795930.3846743070264751No Hit
GATACGAG15717090.382754336352639No Hit
CGTACACT15542560.3785040511965684No Hit
ATCTGCTG13992510.3407560737361474No Hit
TCCGTATG13530840.329513140440994No Hit
AGCAACTA13273870.32325521471730484No Hit
TATGAGGT12972750.3159221151573668No Hit
GAAGGCAG12928630.31484767190356605No Hit
AGAAGAGC12758300.3106996682902417No Hit
GCCACTGT12697100.30920928009593973No Hit
CTAGCGGC11853800.2886726074773964No Hit
CGTGTTAG11850990.28860417625474954No Hit
ATACGTTA11633750.28331378521994305No Hit
TCTTAGTA11591640.2822882909901709No Hit
CACCGGAG11157820.27172358173441796No Hit
CAAGAGAG11110210.27056414738914475No Hit
GAGCGTTA11033090.26868606344233814No Hit
GATCTTCC11011880.2681695416514697No Hit
TACCTGCA10947650.26660536462990087No Hit
AGGCGGTT10889290.265184138240694No Hit
CCTCCATC10780600.26253723803091156No Hit
CGCGGTCG10719050.2610383263747141No Hit
TTGTGGCT10347420.25198811453405334No Hit
AGCCACGC10166590.2475844070638634No Hit
TGAGTAAG9989130.24326276835535313No Hit
ACGCATTA9866870.2402854013515074No Hit
GTTCACTG9789460.23840025510770158No Hit
CGTATGAC9626480.23443124419418307No Hit
TCGTGTCA9246550.2251789045428571No Hit
CTCGAACA6711900.16345321113293096No Hit
CGTGCAGG6217070.15140274070504792No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)