FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408775677
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCC57089971.3966087811041654No Hit
GCTGGAAT54491591.3330438444849055No Hit
CTTGCCGA54456561.3321868952589369No Hit
CAGTGGTC52587561.2864649967908928No Hit
TACCTCGC49083231.2007375380115877No Hit
AGTTACAT47154371.1535512667012229No Hit
ATCGGCGC46723721.1430161486834256No Hit
TAATTAGA45874391.1222387382897052No Hit
CTATTGGA44735691.0943823842043323No Hit
TCCAGATC43168301.0560388601594806No Hit
GGCCGTCA42691151.0443661989213708No Hit
TGATCCAT39118940.9569781716733601No Hit
CACGATAC38764110.9482978606870487No Hit
TGCCGTAG38720380.9472280807940537No Hit
CAGCTAAC38145010.9331526347151031No Hit
TTCAGTAA37778600.9241890387719912No Hit
GTTGCCTT37642160.920851266793939No Hit
TAGGCATT37508980.9175932451577837No Hit
GCAGCTGG37432760.9157286528082736No Hit
TATCAAGA35588180.8706041480056066No Hit
ACTCTTGT34867830.852982013408787No Hit
AAGCATCC34433310.8423522224391057No Hit
ATATTCGG34327180.8397559329343366No Hit
ACTATTAC34161610.8357055451711722No Hit
TGTCGGCT34083880.8338040132461209No Hit
TGGCACAG34037920.832679680205141No Hit
TACACACA34019390.8322263753476703No Hit
CTGTGTTG33899700.8292983635618809No Hit
GTGGTACC33065450.8088898596576722No Hit
CGTGATCC32674150.7993173723005051No Hit
CGACGCCT32579270.7969962948651663No Hit
GATTACGC32219400.7881926888717502No Hit
TATCCGAC32022670.7833800248345011No Hit
TCAGGTAC31890180.7801388828719377No Hit
ATGTAATA31811580.7782160678801836No Hit
AGGTCTTG31779760.777437645831359No Hit
TAGGTGAA31464690.7697299954566523No Hit
GTCTTGTA31323650.7662796923213218No Hit
TCCGACTT31208280.7634573619701938No Hit
AATGTAGG30922820.7564740697622281No Hit
TTAGGATC30424800.744290859556206No Hit
GCGCGAGG30026630.7345503093619732No Hit
TATTGACC29862690.7305397967697574No Hit
TACAGCCT29856400.730385922643827No Hit
AACAGTGA29082400.7114513322670125No Hit
GACTAAGA29063880.7109982720424923No Hit
AGTGTCTC28879030.706476231950562No Hit
AGCTTCAC28224380.6904613358392163No Hit
AGATGACT28065210.6865675131644391No Hit
CCTGGCCA27903960.6826228068359361ABI Solid3 GAPDH Forward Primer (100% over 8bp)
CGGATGCG27878700.6820048640027083No Hit
GTAGAATG27878200.681992632355178No Hit
CGCTTACC27620120.6756791451659684No Hit
CGTCGTGG27602780.6752549516296196No Hit
GATATTGT27489680.6724881529582788No Hit
TCACCGAG27482500.6723125064997446No Hit
GGAAGAAT27184570.6650241570023746No Hit
GATTGGAT26562090.6497962450931247No Hit
CTGAACTA26555650.6496387014729352No Hit
AGGTAGCC26364420.6449605855585189No Hit
TGGTTCCA26356360.6447634114003314No Hit
TGCAATCG26303880.6434795776755572No Hit
CATATCTC26245820.6420592387643456No Hit
CAATCAGC26240680.6419334974277346No Hit
GTGTGTGT26169880.6402014961374525No Hit
ATGGTCAA26074550.6378694102193365No Hit
ATTGCCAG25719200.6291763783195936No Hit
ACGCTATT25647710.6274274973557197No Hit
AATCGCGG25538360.6247524360408557No Hit
ACGGACGG25262970.6180154892141491No Hit
GACTTATC25100080.6140306630817469No Hit
TTAACCAG25034290.6124212228997177No Hit
GTGAGTCA24575530.6011984416577701No Hit
GGATTGTG24483100.5989372992953297No Hit
TCCGATGC24159060.5910102131639305No Hit
AAGCTCCA24117450.5899922954564638No Hit
GTTCAGGA23761730.5812902121375485No Hit
TAACTATG23752560.5810658837218439No Hit
TATAATCC23744860.580877516349878No Hit
TGACGGAA23732850.5805837121762017No Hit
GAGAGCGG23211240.5678234128397028No Hit
TCGTCGAC23044920.5637546776052432No Hit
TAGCCACC22984270.5622709787598248No Hit
CGGAGAGA22648490.5540566935444156No Hit
TGATAAGG22244050.5441627584901535No Hit
TTGTACTC22044770.5392877130505004No Hit
CTCACGCC21968830.5374299704236072No Hit
TCTGTGTT21888440.5354633661336949No Hit
CCAAGACG21147490.5173372876586295No Hit
CAAGCACT20785480.5084813302137837No Hit
TGGCCTAT20747870.5075612656865589No Hit
TAATGATT20533510.5023173137573936No Hit
AAGTGGAG20514360.5018488416569854No Hit
CTGCGCTT19330190.47288014154521235No Hit
ATACAGTC18756910.4588558237529382No Hit
TCCGGCGA18641270.4560268883121439No Hit
GCTGACAA18226250.44587413159614186No Hit
GGATATGC17974700.4397203897236772No Hit
TTGGAACG17844230.4365286636171359No Hit
GCTTCTCA17685140.43263679800596355No Hit
CGAACTGC17589280.43029174654146557No Hit
CCTTACTG17468120.4273277737119374No Hit
GATCGATT17379840.4251681540239979RNA PCR Primer, Index 42 (100% over 8bp)
GGCGCCAT17248130.4219460934315815No Hit
AGCTGTGG16750040.4097611707949052No Hit
CTTCTGCT16425500.4018218530159758Illumina Single End Adapter 1 (100% over 8bp)
CCTGTGCG16418220.4016437602279355No Hit
GCATCGAA16299000.3987272461908246No Hit
GAGCTTAG16258710.39774162003283775No Hit
GAGTAATT16161840.39537186064032864No Hit
AGTCTGAT16116410.39426049314573086No Hit
ACGATTCT16054370.3927427903201785No Hit
CTAGGCAC15713530.3844047208317632No Hit
GCTCACGT15612900.3819429794498267No Hit
AGAACGGT15476390.37860349504112006No Hit
TTGAAGGC15314820.37465096045819773No Hit
GCCGGTTG14718960.36007426146345783No Hit
GTCATACA14569120.356408681331595No Hit
GCTTATAC14562340.3562428201910849No Hit
GCATAACG14378940.35175625187699217No Hit
GGACGTTG14296860.3497483046184277No Hit
CGGCTTGA13631720.33347678854189755No Hit
ATCATGGA13336310.32625008654808No Hit
GCTAGTCT12721030.31119831036326556No Hit
TCATTGCG12678850.3101664485776144No Hit
TCAATCGG12148060.2971815761924602No Hit
CCACCAGG11404760.2789980089740026No Hit
TACGAGCG10730930.2625139068633969No Hit
TTGCAGAA10730090.26249335769554605No Hit
TGTATATT9499750.23239518725083047No Hit
TGGAAGAT9499430.2323873589964111No Hit
TTAGCAGT8335520.20391428524256347No Hit
AGCGTGCG6100290.1492331942245184No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)