FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349387436
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGTAT34263810.980682373478364No Hit
ACTCTTGT33840030.9685531451108047No Hit
ATGTGCAT33766910.9664603394610903No Hit
ACTCCGTT32875180.9409376701227459No Hit
GCTGGAAT32134010.9197242570565703No Hit
TCCGGTCT31857860.9118204239032797No Hit
GACCACTT31133190.8910792659413201ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CGAGCCTT30285260.8668102192432586No Hit
TATACTAT30063800.8604716970990337No Hit
TGCAGTGT29699600.8500477389805167No Hit
CAGTGGTC28777720.8236621307699227No Hit
GCATCCGT28674680.8207129691978964No Hit
ACCTAGGT28667100.8204960180651717No Hit
CAGGCGCA28292020.8097606577930868No Hit
TCCTCCTA28137710.8053440708154141No Hit
TTAAGGAC27810420.7959765330542682No Hit
GGCTGAGT27614380.7903655699857508No Hit
TCCAACCA27028930.773609100242517No Hit
GTGCGGTG26673880.763447029045429No Hit
TGTGAGTC26483810.7580069364600736No Hit
GGTGTACG26449290.7570189215390104No Hit
ATCGCTAA26400970.7556359296216937No Hit
AGAACGGT26381410.7550760926617865No Hit
GTAATCTT26203030.7499705856623876No Hit
GAACAAGT26181880.749365240483347No Hit
CTTATGTA26075910.7463322178534204No Hit
TCTCTGCC25927660.742089077295842No Hit
ATTAATCA25856610.7400555181955656No Hit
CTGCCTTA25610340.7330068961037283No Hit
GTTAAGCC25609810.7329917266973505No Hit
GGACACAT25262770.7230589138872183No Hit
ATACCGAA25130980.7192868835729972No Hit
AATGGTCT24878280.7120542250981229No Hit
TGTGGAGC24819290.7103658415467464No Hit
CGTGTTAG24632720.7050259242865277No Hit
TGGAGTTA24610390.7043868057121551No Hit
TCACGCCG24502620.7013022643435868No Hit
ACAAGTGT24471160.7004018312782145No Hit
CGCCTCAT24469500.7003543195525783No Hit
TCGGCAGC24267410.6945701962791816No Hit
GAGTCCTA23897340.6839782298296496No Hit
GTGATGTC23862170.6829716109196325No Hit
GTGTAGAT23472130.6718080726863915Illumina Single End PCR Primer 1 (100% over 8bp)
GAACAGAC23353250.6684055462143178No Hit
ACGCCTCG23065880.6601805795901602No Hit
AGGATCTT22870790.654596806967037No Hit
TTCTATGA22709210.6499721415282946No Hit
TAGGTCTC22660680.6485831390914698No Hit
ACACTAGC22414540.6415382377974232No Hit
AGTGTCTC22253330.636924162321624No Hit
ATGAATTG22156850.6341627579304254No Hit
TTCCGAGC21820580.6245381989064999No Hit
GTTGCAAC21820120.6245250330066248No Hit
ATGGCGCT21744050.6223477938685809No Hit
ACCTATAA21620170.6188021597891689No Hit
CGGTCGGA21334250.6106186943711394No Hit
GATGGTAC21325890.6103794184516698No Hit
CAGAGTGC21325010.6103542315127782No Hit
GGAGTGCA21292620.6094271804324412No Hit
GGTCCGTG21189370.6064720083409068No Hit
ACGACGAT21181760.6062541985625379No Hit
GGCCTAAG21132370.6048405816172508No Hit
AACCGTTC21008160.6012855024357545No Hit
TTGATGAG21003640.6011561331587206No Hit
ACAGTGCC20925250.5989124920908718No Hit
TTATGACG20915150.5986234147240487No Hit
TGACGACC20861740.5970947392624617No Hit
GCGCAATC20839150.5964481790925075No Hit
CGCGATTA20735250.5934744030120189No Hit
AGACCATC20650730.5910553120175736No Hit
GCACACCA20438200.5849723800600546No Hit
TAGGATCA20412910.5842485417821378No Hit
GGTAATTA20296840.5809264417853881No Hit
GAGAATTC20240870.5793244952288439Illumina Small RNA Adapter 2 (100% over 8bp)
GCATAACG20168440.5772514384289422No Hit
AAGCATCC19830380.5675756468815897No Hit
TCCTTACA19806270.5668855819990046No Hit
TCTCATTC19732210.5647658721191108No Hit
GAAGGCAG19720500.5644307141027247No Hit
ACACGAAT19639370.5621086500660544No Hit
ACTTGTCC19165450.5485443386121074No Hit
TTGTGGCT19051260.5452760470757169No Hit
TCTCTCAG18802050.5381432777107645ABI Solid3 Adapter A (100% over 8bp)
CTATTGGA18659810.5340721524972065No Hit
AGCAGGAA18302650.523849689889822Illumina Paired End Adapter 2 (100% over 8bp)
CCGAAGGT18106380.5182321438713668No Hit
GCAAGCAC18079390.5174596490069551No Hit
GTAATTCC17737560.5076759543236695No Hit
TTAAGTGG17603770.503846680966513No Hit
GGACGTTG17573050.502967427827027No Hit
GTAGCACA17536850.5019313287498981No Hit
TCATCATG17502750.5009553348678514No Hit
AGAGATCG17343710.49640336809363683No Hit
CGCCAGCC16870460.48285823305907316No Hit
CTGACTGG16527830.47305164115861337No Hit
TCCACAGG16512090.4726011384107126No Hit
ACTGTCAT16330590.46740633226433476No Hit
GTTCACTG16299850.4665265066944193No Hit
GCCAACTG16141080.4619822677310011No Hit
CGGACATC16009130.45820565797334506No Hit
AGTCATGA15904500.4552109881821852No Hit
TGTATATT15593760.44631713660132877No Hit
AGCAACTA15357540.43955616080024124No Hit
ACCATAAG14966500.42836400104553274No Hit
CTTCGCAA14900380.4264715460460919No Hit
CGAATCGT14624460.41857429584273886No Hit
AGTAACCG14604870.4180136002371877No Hit
TGAGTAAG14162730.4053588807354824No Hit
AGGACCAG13680640.39156073145114473No Hit
GAAGGTGA13317680.3811722640192477No Hit
CCACCAGG13097580.37487266714421863No Hit
TCAATAAC12945760.37052734775500057No Hit
CATTCCAG12868610.3683191973737716No Hit
ACATACTC12842150.3675618719157377No Hit
GACAGTCG12520740.3583626287008214No Hit
CGTATCCA12281840.3515249472222006No Hit
AACTTGAA12263110.3509888661251116No Hit
TGGCGCGA12185140.3487572460962792No Hit
CCGAATAG11787770.3373839121106805No Hit
AGCGTGCG10730700.3071289604128753No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)