FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001836214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314234259
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGGTT34556721.0997120463558367No Hit
TACTGCAC34092291.0849323084151687No Hit
AACAGAAT33401131.0629372528092171No Hit
TTCCGGTT31979181.0176859805728566No Hit
TAACGGTC31707861.0090516578588589No Hit
TACTGTTG31607911.0058709098297267No Hit
TATGCTTA31310620.9964101336258182No Hit
TCTGATAT31098190.9896498904659533No Hit
CTGTGTTG30162100.9598603314605489No Hit
GTAGTGAC30148700.959433898007919RNA PCR Primer, Index 43 (100% over 8bp)
GGTTCAAT30017840.9552694889324592No Hit
TAAGTCCT29978970.9540325136859124No Hit
TGTGAACA29932090.952540633069547No Hit
TCGTTCTG29530340.9397555853386438No Hit
TCGGATTG29428870.936526465753691No Hit
CTTAACAC28971020.9219561257322996No Hit
GTGGTCGT28770920.9155882649956381No Hit
GAGTATAA27873020.8870140413302294No Hit
TGTTGCGT27823310.88543210051454No Hit
CGAGGAGT27778040.8839914555592743No Hit
GCCACTGT27380620.8713442031156762No Hit
CCTGGTGC27230720.8665738766567779No Hit
TGCGGATT27208790.8658759896704961No Hit
TGAGCGTA27117200.862961285198378No Hit
GTCACAAT26804540.8530113834596247No Hit
ACTACATA26533300.8443796066169857No Hit
GTCGAAGC26459380.8420272214812835No Hit
CGCGTCGG26346320.8384292687831979No Hit
TACATGTC26271680.8360539708052648No Hit
CCGCTGTA25664610.8167349442315264No Hit
GAGGTATA25657520.8165093163823363No Hit
TGGCTGTG25545840.8129552799651931No Hit
TTCGCATA25421000.8089824477094968No Hit
AATATTCG25328420.8060362380793114No Hit
TGCTCGAG25219530.8025709889258128No Hit
CTTCGACG24548760.7812248122824825No Hit
GCTGCATG24446680.7779762804284176No Hit
GTCCGACT24417200.7770381268326316No Hit
CATACGTT24363130.7753174360278775No Hit
AAGGTGTT24321500.7739926282194456No Hit
CGCAAGTG24316860.7738449676806245No Hit
TACAGCCT24141740.7682720552758062No Hit
GTGGACAC23837850.7586012446847815No Hit
GTCTCCAC23740000.7554873257788228No Hit
AGGTTATC23700710.7542369847076413No Hit
AGTGGATG23583780.7505158754825648No Hit
TCGTGAAG23531110.7488397374265929No Hit
AAGAACGT23461930.7466381951689106No Hit
TAACCTCT23441010.745972449808536No Hit
TACAAGAA23404920.7448239435917139No Hit
AATTAACA23315840.7419891158334839No Hit
CACCACGG22928870.7296744178361532No Hit
CGTAATGT22766600.724510436018372No Hit
TTAGGTCA22703230.7224937876681359No Hit
AGTCTACC22524390.7168024922451247No Hit
GCACCACT22518650.7166198259751175No Hit
TCGCACGC22455920.7146235445957533No Hit
TGTAGCAC22424400.7136204712803131No Hit
AGCGAAGA22288700.7093020369876347No Hit
CTCTGAGA22246740.7079667274598471No Hit
CGCCGGTA22051060.7017395261157695No Hit
GTTATCCG21929890.6978834857086668No Hit
TGATCCAT21841460.6950693431552287No Hit
TATGAGGT21833820.6948262124404456No Hit
CGAGTTCT21649420.688957978957985No Hit
ATCTGCTG21632460.6884182542298801No Hit
CACCGGAG21100200.6714799356107126No Hit
CGCACCGA20927310.665977989370026No Hit
TCTATGAA20877440.6643909568116186No Hit
ATCTAACC20749180.6603092885553259No Hit
AATCGCGG20448910.6507536786432953No Hit
GTTACCAA20428200.650094616195238No Hit
TCCGTATG20247270.6443368098829733No Hit
ATTCCACA20164850.6417139259153789No Hit
TCATTCAA20074070.6388249983907707No Hit
CCTACTCG20053170.6381598894982358No Hit
CCGACACA19816890.6306406584394734No Hit
CAAGACGC19392800.6171446761315735No Hit
CACATTAT19338270.6154093465665054No Hit
ATAGCCTA19105770.6080104079294549No Hit
TTGCTCTA19095690.6076896281382228No Hit
AGAAGAGC19043940.6060427676028793No Hit
TACCTGCA18946980.6029571715157894No Hit
GTATATCA18828990.5992023294952062No Hit
AGGCGGTT18411540.5859176545101024No Hit
CACACCAC18365840.5844633254962821No Hit
AACGCGCC18046370.5742967064580949No Hit
GAGACTGA17397670.5536528720759247TruSeq Adapter, Index 15 (100% over 8bp)
GTGCCACG17019670.5416236299047202No Hit
ACTTAAGG16669940.530494035024997No Hit
TGAATTGA16130490.5133269062174408No Hit
GATCTTCC15751110.5012537477652939No Hit
CAGATAGT15574100.49562068914961943No Hit
GCGCCTAA15419150.4906896545611852No Hit
CTAGTCCG15396160.48995803477939687No Hit
CTCATAGG15318050.48747230963126786No Hit
GCTGACAA15047590.478865355034379No Hit
TATAGGTG14038510.44675300664782064RNA PCR Primer, Index 39 (100% over 8bp)
CGGAGCTG14006670.44573974984694464No Hit
CTTCTAGA13872680.44147573355456443No Hit
TAGCATGG13019180.4143144684933924No Hit
CCGCGTAC12661730.4029391970275272No Hit
AATTATTG11891310.3784218193726611No Hit
CACACATG11366890.3617329961466741ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CGATGGCA11191690.3561575378704968No Hit
AGGTAGCC10766450.34262495866181164No Hit
CGACCTCG9969810.3172731716690382No Hit
CGTGCAGG8946280.28470097526826316No Hit
TCGTGGAG4252690.13533502087052832No Hit
TCGGGTTG3351920.10666946407011592No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)