FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001836216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325240798
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCC39419361.2120053893115832No Hit
GCGTCGCT32009040.9841643544362476No Hit
CTTGCGAT32000550.983903317074016No Hit
CTTGCCGA31385350.9649881009085459No Hit
GTGGTACC30576470.9401179122675747No Hit
GATGGCGT29267850.8998824926016816No Hit
ATTAGTGC28986340.8912270594047675No Hit
TCTCGAGT28947890.8900448583944256No Hit
AGAGCCGC28113050.86437649190616No Hit
GCGGCTCC27976560.8601799089178228No Hit
CGTGTGGT27241100.8375671246508256No Hit
CCGGTAGG26905780.8272572249684371No Hit
TTCGACAA26836640.825131415401336No Hit
CGATGCTC26505990.8149651016413999No Hit
GGAATATC26269800.807703097567729RNA PCR Primer, Index 37 (100% over 8bp)
GCATGTAT26251510.8071407449934986No Hit
CACTTGCT26194490.8053875824028695No Hit
AGTTACAT26109340.802769522168003No Hit
CATCTCCG25798880.7932239792376847No Hit
TATCACAT25720970.7908285233022949No Hit
CCAGCGTG25709400.7904727868734353No Hit
ATTCTGGC25220940.7754543758068138No Hit
GGTCTCGC25195510.7746724935781273No Hit
ACTGGTTA25194480.7746408247344173No Hit
ATATACAA25120470.7723652799548228No Hit
TTATGCTA25062080.7705699947274143No Hit
CTCTAGTT24980840.7680721531128454No Hit
TATTGACC24809580.7628065160509169No Hit
ATTCGATC24733150.7604565648618289No Hit
TGCGCCTG24702550.759515723485588No Hit
ATAGTTGC24458350.7520074403457835No Hit
TCGGAGCT24212390.7444450434536198No Hit
GGTAGCGA24145420.7423859536834613No Hit
GTCGACCG23962710.7367682697666976No Hit
TGGCACAG23824460.7325175730260014No Hit
CCACATAT23536170.7236536788967047No Hit
AGCAAGCT23492710.722317438170841No Hit
GCAGATCT23385880.719032794895553No Hit
AGTGCTCA23284300.7159095704838357No Hit
AAGATGGC23127710.7110949838463992No Hit
GCGTACGA23111800.7106058078236545No Hit
GCCTGGTC23071040.7093525825133413No Hit
TGCTCAGC23037870.7083327227600763No Hit
CCACTACA22968780.7062084505154854No Hit
GATATTGT22828480.7018947235518712No Hit
GCAGTTAA22537680.6929536558325625No Hit
CGTTGTCT22340120.6868793871302702No Hit
TCAACTTA22139250.6807033476778027No Hit
CCGGCTGA22011780.6767840976703051No Hit
CGGATGCG21986520.6760074423381535No Hit
CTCCAATG21975860.6756796851789793No Hit
ATAAGCCA21920110.6739655705801091No Hit
GCTTATAC21905210.6735074484720702No Hit
CTTGCTTG21597660.6640513777118453No Hit
CTTACAAG21396280.6578596575697738No Hit
CTGTCATT21392630.6577474330265295No Hit
CGGCTTGA21316050.655392869869911No Hit
TCGCAACA21239010.6530241633461987No Hit
TCCGCGAT20960670.6444661963964312No Hit
TCTCCACG20901050.6426330930352717No Hit
GACATCTA20779150.6388851007554102No Hit
CATTGTGA20686250.6360287555314632No Hit
GGTCGTAT20664160.6353495664464579No Hit
GCTAGTCT20554630.6319819077556192No Hit
ATCGGCGC20514910.6307606587535184No Hit
ACCGACAC20476460.6295784577431766No Hit
ACACTGTG20023820.6156613845228605No Hit
GCAGTGGT20008470.6151894265122299Clontech Universal Primer Mix Long (100% over 8bp)
AACAATAG19993950.6147429880552685No Hit
AGCTCACG19964610.6138408872062846No Hit
TCCGGCGA19909840.6121569041286142No Hit
GATACACC19898750.6118159259958524No Hit
GCGGAGTA19778040.6081045219917336No Hit
GATTGAGG19704210.605834511573176No Hit
CAGGCTAG19481300.5989808203582134No Hit
ATGGAGAG19477800.5988732077824996No Hit
CGTAGTTC19468240.5985792717185499No Hit
CAATCAGC19460120.5983296105428938No Hit
AAGTGAGA19051830.585776142389123No Hit
TCTTAGTA18985430.5837345780955807No Hit
AGGCCAGA18932500.5821071684862856No Hit
CCATGCGG18538300.5699869178158885No Hit
TGCTAACT18418390.5663001109719328No Hit
GTTCTAAT18356700.5644033624588511No Hit
ATCATCTC18209820.559887323852895Illumina PCR Primer Index 7 (100% over 8bp)
AGAAGTAG17864530.549270882061973No Hit
CAACTCGA17862690.5492143085935978No Hit
TGTGTCGA17828770.5481713890026798No Hit
TATACGGA17739440.5454248086059609No Hit
AGCGTTAT17692400.5439784955883671No Hit
AGATGACT17541930.5393520772261787No Hit
TAGCGCTG17284480.5314364036211718No Hit
GAACGCGC16971610.5218167617458619No Hit
GCTACCTC16746170.5148852820118833No Hit
TCATTGCG16682880.5129393391784755No Hit
AAGCAGGA16674880.5126933675768438No Hit
ACTCATAG16394040.5040585345015665No Hit
CCATCTCC16209880.498396268232007No Hit
ATGAGACG16186000.4976620430011366No Hit
CTCAATTC15991380.4916781688624439No Hit
CTCTCGAA15988730.49159669076940343No Hit
TACGAGCG15873980.4880685356085001No Hit
AGACTCAA15836070.4869029376812684No Hit
GTAAGAGA15791630.48553656543420487No Hit
ACCTCGCA15246290.4687692962799827No Hit
AACCAACG15090980.4639940650988072No Hit
CTGATTAC14844070.4564024590789499No Hit
TCGTGTCA14579100.44825557216840917No Hit
GGCAACAC14193390.43639635886024364No Hit
CGACTTAC14072000.4326640472699861No Hit
AGCCACGC13901990.4274368432708125No Hit
ATACTTAG13678690.42057116094027047No Hit
AACACGTA13555890.4167954968552254No Hit
AACGTCGA10740350.3302276364479957No Hit
ATCTCAGT10644450.32727905187343687No Hit
GATCGGCG9904830.3045383623735913No Hit
GCGAGATA9556700.2938346006640901No Hit
CGTATGAC9311020.2862808127779837No Hit
TACGTACC6818280.209637906496589No Hit
CTTGCGGT3375100.10377234408335205No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)