FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001836218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331326657
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAAGT35865761.0824894176866668No Hit
CACTGCTT33824961.0208946151893838Clontech Universal Primer Mix Long (100% over 8bp)
TCTGTGTT32911400.9933218262000573No Hit
TTCACGGT32193060.9716411076456188No Hit
GACTCGGT31537430.951853083164389No Hit
TCTAGTGA30962570.9345028341622388No Hit
GCAGCTGG30850670.9311255025278573No Hit
CTATCGCT30506700.9207439050097318No Hit
TCGATTGC30359690.9163068940752328No Hit
GTCTTGTA30192280.9112541765693184No Hit
TGATTCGC28657550.8649334242973393No Hit
GTCCGCGG28192920.8509101034994597No Hit
TTACAATT28136740.8492144958924932No Hit
GCCATCGC27835070.8401095840592143No Hit
CATCAGTG27706790.8362378762660199No Hit
GAGTTCCT27544530.8313405944876932RNA PCR Primer, Index 1 (100% over 8bp)
GGCTACGG27490550.8297113866090164No Hit
GCCTACAT27403200.8270750155789607No Hit
GCACTCTC27068370.8169692787501851No Hit
TACTCAAT27049330.8163946192835309No Hit
TACCTCGC26905690.812059320660094No Hit
ACAGGCTG26881680.8113346581708938No Hit
CTATCCTG26637070.8039519138358976No Hit
GGTTGGCC26618340.8033866106945932No Hit
TGCCGTAG26328210.7946299956178895No Hit
ACGTGGTA26279680.7931652779752039No Hit
TATGCCGC26273080.7929660787903341No Hit
CACGCCGT25277420.7629153726680072No Hit
GCCTTAGG25267180.7626063121145124No Hit
GTACTATA24973420.7537401374861306No Hit
GACCTGTG24898310.751473190398924No Hit
GGCCGCTC24694100.7453097865288878No Hit
GGACCGGC24430830.7373638517712144No Hit
CGTACACT23882080.7208016468170866No Hit
CCTTGTAG23862870.7202218564623372No Hit
GATTACGC23815870.7188033168125074No Hit
TTCAGTAA23801470.7183687004091555No Hit
GCCGGTTG23603280.712386990341076No Hit
TGTCTTAA23568520.7113378746340956No Hit
CGACTCTG23262070.7020886943002597No Hit
TCGTCGAC23258450.7019794365655282No Hit
AGAATGTA23090180.6969007628021914No Hit
GTGTCGGC22978100.6935179984627678No Hit
TACACACA22904360.6912924002972691No Hit
GGCCGTCA22808820.688408841187807No Hit
ATTACGAC22800490.6881574276711457No Hit
GGCGGAAC22702100.6851878507318534No Hit
TTGACCGA22564000.6810197586969285No Hit
GCGATCCA22557530.6808244831323669No Hit
GCTGTAGC22409540.6763578941370841No Hit
CAGTACCG22372300.6752339278273043No Hit
CGTGATCC22347140.6744745563892253No Hit
TTAGAGAT22308720.6733149756797263No Hit
ACCAGGCC22295280.6729093337032643No Hit
TAATTAGA21736710.6560507445074062No Hit
CCAGGATA21696510.6548374403813817No Hit
TAGATCCG21641970.6531913307536857No Hit
CCTCCATC21608920.6521938257446035No Hit
GGCTCTTA21541350.6501544486352633No Hit
GACAAGGC21466380.6478917269853116No Hit
ATACATGT21404020.6460095965052398No Hit
CTCGGCCT21218820.6404199466510176No Hit
AATCTGTA21192520.6396261680810066No Hit
AACGGCCG21171620.6389953706622524No Hit
AGATGGAC21018370.6343700259529677RNA PCR Primer, Index 16 (100% over 8bp)
TTACAGCG20957890.632544637058889No Hit
ATTAGGCT20815320.6282416328487569No Hit
CGTTCATA20766990.6267829515450065No Hit
CAATACAT20681940.6242159984127085No Hit
AATCAGCT20445240.617071991282609No Hit
GATACGAG20412660.616088671670025No Hit
TTAGGATC20153970.6082809690739734No Hit
CGCGGTCG20149680.6081514896038082No Hit
CCATAAGA19941520.6018688680397968No Hit
GGATATGC19891400.6003561615025741No Hit
ATCGCGTG19822550.5982781518240472No Hit
AGGTCTTG19817220.5981172833914176No Hit
GGAACCTG19216880.5799980048088916No Hit
ATTGACCT18987730.5730818694736053No Hit
GTCCATCC18949030.5719138378895967No Hit
TATCAAGA18809200.5676935315228802No Hit
AACGGATA18599790.5613731828405222No Hit
GCTTCTCA18476080.5576394053920025No Hit
TTATCGTC18475260.5576146564023673No Hit
GAGCTTAG18401440.5553866437012944No Hit
CGACGCCT18331800.5532847904839724No Hit
CTCGTATC18004160.5433960600399261No Hit
CAGCACAA17990170.5429738181313917No Hit
CCTGTGCG17660740.5330310624538731No Hit
GAGAAGCT17509220.5284579320763798No Hit
CATGTATT17498250.5281268388857707No Hit
ATAATATG17366060.5241371206663881No Hit
GGCGTGTC17194890.5189709199884874No Hit
ACGCATTA17109290.5163873669241168No Hit
TCAATCGG17082470.5155778938728736No Hit
CCATTGAC16762600.5059236751964693No Hit
CAAGAGAG16652670.5026058015006019No Hit
CCTATACC16553520.4996132864733549No Hit
TGACGGAA16547510.4994318944883448No Hit
TTAACCAG16374760.49421800673285393No Hit
CATGACTA16289620.491648337248035No Hit
GTATCTAG16026810.4837162860699132No Hit
ATACGTTA15956800.4816032656255606No Hit
AAGTGGAG15817580.47740137009259714No Hit
CTACAAGC15803030.4769622264350435No Hit
TTGCAGAA15099530.455729404229615No Hit
CTAGGCAC14834140.447719484279226No Hit
GTGTTAAG14797400.4466106088167847No Hit
GTCATACA13995010.4223931188247253No Hit
CGATCTAA13903870.4196423591718429No Hit
GACTAAGA13808320.41675849824543393No Hit
ACCGAACT13791470.4162499366901227No Hit
CTCGAACA13745170.4148525242265671No Hit
CTAGCGGC12280890.3706580723445986No Hit
TTAGCAGT12280090.3706339269888568No Hit
AAGCCTGT11857310.35787371011321917No Hit
AATTCATT10704210.32307119798090983No Hit
ATGTCAAC10469830.3159972123824616No Hit
ATGCGGCA9274830.2799300872431765No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)