FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22311695
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGA4122901.8478649873978645No Hit
GGTACC4105531.8400798325721106No Hit
GAAGAT3998651.7921767037421406No Hit
GATCCT3968531.7786770570321977No Hit
CGTACT3748721.6801592169487796No Hit
TGCGAC3631041.62741557734632No Hit
CTATTA3443081.5431727620873268No Hit
ACCAGG3416741.5313672941477552No Hit
CCTTAT3394541.5214173553376378No Hit
AGCCTA3335391.49490659494942No Hit
AGAACG3304171.4809139332533903No Hit
AACCTT3240221.4522518347440658No Hit
CCGGAA3220671.4434896138549762No Hit
TCTTCA3220371.4433551552224069No Hit
TCGTGG3218911.442700789877237No Hit
TTCACA3209491.4384787888145656No Hit
ACGAAC3160781.4166471888397543No Hit
CGAGAG3134371.4048103472192497No Hit
CACCTA3109471.39365028071601No Hit
ATGTGA3105461.3918530169940024No Hit
AGGTGT3026511.3564679868562204No Hit
CCATGA3010021.3490772440193362No Hit
TCCTCC3006461.347481668246182No Hit
AACATA3004921.3467914472656604No Hit
CGAGCC2991771.3408976772047125No Hit
GGCCGT2900421.2999550235874058No Hit
GGATCG2887501.294164338478094No Hit
GCGTAT2865071.2841113147163403No Hit
GCACAT2859471.2816014202417163No Hit
CTCCGC2849421.2770970560506496No Hit
CCGCTC2839021.2724358234549191No Hit
GACACG2805711.2575064332853243No Hit
AAGCAT2781891.2468304178593335No Hit
ACCTGC2768951.2410307688411841No Hit
TGCATA2723081.2204720439213605No Hit
GCTGTC2716611.2175722194122858No Hit
CGTCTC2672341.1977306071994978No Hit
TAATGA2662121.1931500497833087No Hit
CCAGCG2552331.143942672217418No Hit
TCTCGC2551571.143602043681576No Hit
AACAAT2506371.1233436097078235No Hit
GGTGGT2481021.1119818552557301No Hit
AGGTCA2462511.1036857576262136No Hit
GGCCAG2460001.1025607870670517No Hit
TACTCG2440921.0940092180356535No Hit
AGAGGT2417431.0834811071054888No Hit
ATGCCG2374261.0641325098787877No Hit
TCCAGA2369131.0618332672618553No Hit
TCCACG2352401.0543349575189156No Hit
GCGGCT2305441.0332876995674243No Hit
GCGCAG2272341.0184524304406277No Hit
CATGAG2257381.0117474266298458No Hit
CATACG2254821.0106000462985891No Hit
TTAGTA2230990.9999195489181795No Hit
CCGACT2228580.9988393979032072No Hit
AGGCTT2198950.9855593669597938No Hit
GACTAA2177750.9760576235915739No Hit
TCAAGC2162220.9690971483789107No Hit
GTCGAG2161980.9689895814728554No Hit
CAGGAC2160710.968420373261646No Hit
CTGTTC2133320.9561443001080824No Hit
CTACCG2113430.9472296927687476No Hit
AGACGG2109990.9456879004486213No Hit
GAATAC2078570.931605599664212No Hit
TATTGG2064050.9250978018478649No Hit
ACACGA1993010.8932579976554897No Hit
TAAGTC1979830.8873507817312849No Hit
ATGGCC1959830.8783868728933414No Hit
TGGCAT1927160.8637443278065606No Hit
AAGCGC1924990.8627717436976438No Hit
GGCGTT1922210.8615257603691697No Hit
ATCCGG1913000.8573978803492966No Hit
CAGTAG1858710.8330653498086991No Hit
AAGAGG1841630.8254101716610953No Hit
GTTGGC1765940.791486258663898No Hit
AGATGC1679260.7526366777602508No Hit
GGTAAT1661140.744515376353074No Hit
CTATGC1555540.6971859376887323No Hit
GCTCAA1527590.6846588750877063No Hit
GAGCCA1510930.6771919390256993No Hit
CTTAGC1465400.6567856005561209No Hit
CAAGCT1413500.6335242571216575No Hit
CCTGCT1369170.6136557531823558No Hit
CATAAC1339230.6002367816519543No Hit
TGACCA308720.138366896822496No Hit
CGATGT275480.12346888033383388No Hit
ACAAGT268440.12031358442287778No Hit
GCCAAT262390.11760200199939987No Hit
ACAGTG240150.10763413537160668No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)