FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21883067
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGA4064021.8571528387679843No Hit
GGTACC4020791.8373978382463483No Hit
GAAGAT3893801.7793666673871629No Hit
GATCCT3891261.778205952575112No Hit
CGTACT3654711.6701086735236885No Hit
TGCGAC3580671.636274293726743No Hit
CTATTA3382031.5455009117323453No Hit
ACCAGG3342741.5275463900923942No Hit
CCTTAT3321721.5179407895611707No Hit
AGCCTA3278661.4982634746765617No Hit
AGAACG3221161.47198745038801No Hit
CCGGAA3178981.4527122729185997No Hit
AACCTT3171941.44949517359701No Hit
TCGTGG3164031.4458805066035763No Hit
TTCACA3159681.4438926682443554No Hit
TCTTCA3139271.4345658220577582No Hit
ACGAAC3081581.4082029726454706No Hit
CGAGAG3074821.4051138261378078No Hit
ATGTGA3062861.3996484130857891No Hit
CACCTA3048541.3931045406021012No Hit
AGGTGT2966651.3556829122718492No Hit
TCCTCC2946331.3463971937754429No Hit
CCATGA2938511.3428236544722No Hit
AACATA2937821.3425083421807373No Hit
CGAGCC2932611.3401275058930269No Hit
GGCCGT2909651.3296353751510244No Hit
GGATCG2822441.289782643356162No Hit
CTCCGC2811201.2846462518256696No Hit
GCGTAT2809811.2840110574993897No Hit
CCGCTC2802981.2808899227882453No Hit
GCACAT2797741.2784953772704712No Hit
ACCTGC2749351.2563823891778971No Hit
GACACG2741181.2526489088572457No Hit
AAGCAT2724091.2448392174643526No Hit
TGCATA2668491.219431444413162No Hit
GCTGTC2666171.218371263954911No Hit
CGTCTC2620741.197610919895278No Hit
TAATGA2613521.1943115651933067No Hit
TCTCGC2515041.1493087326378886No Hit
CCAGCG2507631.1459225528121812No Hit
AACAAT2456171.122406653509766No Hit
GGTGGT2445071.1173342383862372No Hit
AGGTCA2417951.10494109440875No Hit
GGCCAG2415531.1038352165169536No Hit
TACTCG2407211.1000331900459839No Hit
AGAGGT2366161.0812743935756355No Hit
ATGCCG2333971.066564389717401No Hit
TCCAGA2322451.061300045372982No Hit
CATACG2309231.0552588446582922No Hit
TCCACG2307511.0544728488013129No Hit
GCGGCT2291801.047293781991345No Hit
GCGCAG2240121.0236773483351305No Hit
CATGAG2201591.0060701271901238No Hit
TTAGTA2178440.9954911713243851No Hit
CCGACT2173840.9933890893813011No Hit
AGGCTT2160780.987421004560284No Hit
GACTAA2140440.9781261465771687No Hit
TCAAGC2120520.9690232178149435No Hit
CAGGAC2118370.9680407229937192No Hit
GTCGAG2116320.9671039256060405No Hit
AGACGG2104840.9618578602350393No Hit
CTGTTC2092970.9564335748732113No Hit
CTACCG2078500.9498211562392054No Hit
GAATAC2043100.9336442647641668No Hit
TATTGG2019850.9230196114648828No Hit
ACACGA1964670.8978037676345825No Hit
TAAGTC1939740.8864113974517375No Hit
ATGGCC1917960.8764584964255697No Hit
AAGCGC1899540.868041029166524No Hit
TGGCAT1897480.8670996620354907No Hit
GGCGTT1893610.8653311713572873No Hit
ATCCGG1880480.8593310983327886No Hit
CAGTAG1818230.8308844459508349No Hit
AAGAGG1805310.8249803375367813No Hit
GTTGGC1735120.7929053089313304No Hit
AGATGC1656200.7568408943773741No Hit
GGTAAT1617930.7393524865595851No Hit
CTATGC1539140.7033474786692377No Hit
GCTCAA1500620.6857448272675855No Hit
GAGCCA1488130.6800372178177767No Hit
CTTAGC1442760.6593042922182709No Hit
CAAGCT1385190.6329962797262376No Hit
CCTGCT1341320.6129488156299114No Hit
CATAAC1318050.6023150228439186No Hit
TGACCA304570.13918067334894144No Hit
CGATGT278500.12726735242367992No Hit
ACAAGT263930.12060923635612869No Hit
GCCAAT258660.11820098160829101No Hit
ACAGTG236640.10813840674161442No Hit
TTAGGC221740.10132948914336369No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)