FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20699307
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGA3859981.8647870675090716No Hit
GGTACC3740541.8070846526407864No Hit
GATCCT3654201.7653731112833873No Hit
GAAGAT3595121.7368310929443194No Hit
CGTACT3428841.6564999011802666No Hit
TGCGAC3401091.6430936552610191No Hit
CTATTA3165601.5293265615124216No Hit
ACCAGG3116141.5054320417586926No Hit
CCTTAT3080621.4882720469820558No Hit
AGCCTA3055131.4759576250547906No Hit
AGAACG3021451.4596865489264932No Hit
TTCACA2985511.4423236488062137No Hit
CCGGAA2976651.4380433122712757No Hit
TCGTGG2964581.4322121991813543No Hit
AACCTT2941851.4212311552265977No Hit
TCTTCA2937391.4190764937202969No Hit
CGAGAG2896301.3992255876005897No Hit
CACCTA2893691.397964675822239No Hit
ACGAAC2889771.3960708926149072No Hit
ATGTGA2858741.3810800525834028No Hit
TCCTCC2812981.3589730322855735No Hit
AGGTGT2769301.3378708765467366No Hit
CCATGA2754451.3306967233250853No Hit
CGAGCC2739131.3232955093617387No Hit
AACATA2723171.3155851063033175No Hit
GGCCGT2697671.3032658532964414No Hit
CTCCGC2650751.2805984277637894No Hit
CCGCTC2649641.2800621779270196No Hit
GGATCG2649171.2798351171853242No Hit
GCGTAT2638151.2745112674545094No Hit
ACCTGC2610621.261211305286694No Hit
GCACAT2597521.254882590996887No Hit
AAGCAT2575591.2442880334109736No Hit
GACACG2539031.2266256063548409No Hit
TGCATA2495491.2055910857305512No Hit
GCTGTC2483761.1999242293473882No Hit
CGTCTC2481711.1989338580272277No Hit
TAATGA2457551.187261969688164No Hit
TCTCGC2371601.145738840435576No Hit
CCAGCG2347301.1339993169819647No Hit
AACAAT2294341.108413919364547No Hit
GGTGGT2275171.0991527397511425No Hit
AGGTCA2267401.0953989908937531No Hit
CATACG2265121.09429750474255No Hit
TACTCG2253481.0886741280758818No Hit
GGCCAG2249161.0865871016841289No Hit
AGAGGT2237271.0808429480271973No Hit
GCGGCT2218201.071630079209898No Hit
TCCAGA2170861.0487597483336035No Hit
ATGCCG2166801.0467983300117245No Hit
TCCACG2142241.0349331984882393No Hit
GCGCAG2063720.9969995613862822No Hit
AGGCTT2057880.994178210893727No Hit
CATGAG2051090.9908979078381706No Hit
CCGACT2048620.9897046311743674No Hit
TTAGTA2027310.9794096005243074No Hit
AGACGG2003780.9680420702007076No Hit
TCAAGC2001350.9668681178553465No Hit
GACTAA2001100.9667473408650831No Hit
CAGGAC1981310.9571866343158252No Hit
GTCGAG1962180.9479447790208628No Hit
CTACCG1961830.9477756912344939No Hit
CTGTTC1946860.9405435650575161No Hit
TATTGG1911070.923253131131395No Hit
GAATAC1897940.9169099236027564No Hit
TAAGTC1836190.8870780070076743No Hit
TGGCAT1833400.8857301357963338No Hit
ACACGA1826580.8824353395019456No Hit
AAGCGC1805610.8723045655586441No Hit
ATGGCC1804850.871937403508243No Hit
GGCGTT1776920.8584441981560059No Hit
ATCCGG1752190.8464969382791414No Hit
CAGTAG1680390.8118097866754669No Hit
AAGAGG1675470.8094328955070815No Hit
GTTGGC1649270.7967754669274676No Hit
AGATGC1573180.7600157821708717No Hit
GGTAAT1498890.724125691744173No Hit
CTATGC1461770.7061927242298498No Hit
GCTCAA1403210.6779019220305298No Hit
GAGCCA1396250.6745394906215942No Hit
CTTAGC1345510.6500265926777162No Hit
CAAGCT1316490.6360067996479303No Hit
CCTGCT1262570.6099576183879006No Hit
CATAAC1250980.6043583971192852No Hit
TGACCA307010.14831897512317682No Hit
CGATGT280620.13556975603096277No Hit
ACAAGT267540.12925070390037696No Hit
GCCAAT260500.12584962385455706No Hit
ACAGTG237180.11458354620277866No Hit
TTAGGC231330.11175736463061299No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)