FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28371319
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA3941871.3893855269823725No Hit
GGTACC3690671.300845406588252No Hit
AATCGG2944801.0379496279323495No Hit
CATAAC2694090.9495822171679787No Hit
TAATGA2583150.9104793471181231No Hit
TCTCTG2551000.8991474805947512No Hit
ATTGCT2492720.8786056087135039No Hit
GCCAAT2406980.8483849481936317No Hit
TAAGTC2343270.8259291716398522No Hit
ACCGCT2330040.821266011636611No Hit
AAGCAT2308220.8135751460832681No Hit
GAGCCA2296820.809557003676847No Hit
TGGCAT2223390.7836752320186453No Hit
TCAAGC2222750.7834496520940744No Hit
TGTGTG2164370.7628725333496127No Hit
TTACGT2121010.7475894934599269No Hit
CAGTCC2096580.7389786847766929No Hit
AGTCAA2092360.7374912671490529No Hit
TATTGG2069060.7292787480201396No Hit
CGAGAG2068720.7291589086852113No Hit
TGCGAC2067220.728630205736998No Hit
GACTAA2062580.7269947512838582No Hit
GGCTAC2032790.716494710732342No Hit
TATTCT2013050.709536979933855No Hit
GATCCT1991520.7019483302838335No Hit
GGATCG1964770.6925197943740297No Hit
CGATGT1961210.6912650060436034No Hit
ATCCAC1892190.6669376210531488No Hit
ACAGTG1875250.66096680242466No Hit
CGTACT1873120.660216044238197No Hit
AGTGCA1848380.651495970278999No Hit
TGACCA1843350.6497230530593238No Hit
GAAGAT1836290.6472346245163998No Hit
GAGATA1809230.6376968233306319No Hit
TTCACA1808660.6374959162103109No Hit
GTAAGA1807630.6371328735192043No Hit
AACTAG1795250.632769311853284No Hit
CAGATC1770460.6240316144624788No Hit
AATTGC1763560.6215995809006977No Hit
TACGGC1754050.6182476042090254No Hit
CTATGC1718040.6055552087655848No Hit
TCTTCA1717540.6053789744495136No Hit
TTCTGT1704990.600955493116129No Hit
ACACGA1692590.5965848820775658No Hit
CCGTCG1684190.5936241455675713No Hit
GCATAA1677170.5911498157699331No Hit
AGACGG1667480.5877343947244751No Hit
CAAGCT1666570.5874136482692257No Hit
CCAGCG1621280.5714503439195054No Hit
GTCTCA1616330.5697056241904016No Hit
ATCAGC1615320.5693496308719379No Hit
AGATGC1582110.5576441475984956No Hit
TTCTAC1573680.5546728370295368No Hit
GGATTC1571640.5539538010199667No Hit
TTAGGC1566390.5521033407012201No Hit
TTCCAT1562020.5505630527787587No Hit
CGAATT1552240.547115909556408No Hit
TGCATA1545490.5447367462894481No Hit
CGTCAG1542000.5435066307632719No Hit
ACTAAT1533070.540359085878242No Hit
AGAACG1514650.5338666136741826No Hit
TCGGAC1513530.5334718488061835No Hit
AAGTTG1505650.5306943959849029No Hit
GTACGC1499470.5285161398382641No Hit
ACTTGA1498930.5283258067769073No Hit
AGGTCA1492940.5262145196703756No Hit
ACCTGC1486480.523937572306737No Hit
ATGTGA1483330.522827296115489No Hit
TATAAG1474700.5197854918201019No Hit
ATACCT1465470.5165322063454293No Hit
GGACTA1459480.5144209192388975No Hit
TAGCTT1454960.5128277610216148No Hit
TACTCG1444530.5091515131883717No Hit
AACCTT1440050.5075724537163746No Hit
AGGCTT1421390.5009953890406013No Hit
GAACCG1417520.4996313354342109No Hit
GAGAAG1415400.4988841019340694No Hit
AGTTCC1407440.4960784516222175No Hit
GATCAG1396940.4923775309847244No Hit
ACGCTA1394550.49153513095390455No Hit
GTTACA1386590.4887294806420526No Hit
CACATT1384070.48784125968905423No Hit
ACAGCC1379540.48624457678545013No Hit
CATACG1364970.4811091088151383No Hit
AGTTAG1358190.4787193714892142No Hit
AGCCTA1353150.4769429295832175No Hit
CTGGAG1350760.4761005295523976No Hit
CACCTA1350440.4759877395901121No Hit
GCTGTC1348570.4753286232480062No Hit
CGCTTG1342360.4731397930424031No Hit
GGAGGC1322800.46624550659770175No Hit
CCGACT1318450.4647122680478831No Hit
AGTAGG1316240.46393331237084884No Hit
CTAGTT1304490.45979180594317803No Hit
CAACGG1296290.456901563159612No Hit
GCGCAG1296080.45682754474686216No Hit
GACCGA1295500.4566231129402197No Hit
GCACAT1293230.45582300914525686No Hit
GGCGTT1292150.4554423430225433No Hit
TAGAGC1286710.4535249136636897No Hit
CTTAGC1276940.45008129512766043No Hit
CTGTTC1271320.44810042141502127No Hit
TACGAA1269230.4473637619738441No Hit
ATCCGG1262840.44511148741445544No Hit
CTGTAT1256760.44296847813103085No Hit
CGACAA1253250.44173131323221176No Hit
CTACCG1252600.4415022086213193No Hit
GACCAT1232680.43448103346904665No Hit
TTGAAT1228520.4330147639593352No Hit
CCTGCT1209260.42622621810427636No Hit
GATTCA1197340.42202479200914134No Hit
CGAGCC1186200.4180982914470772No Hit
GTGGTG1167760.4115987698703751No Hit
ATTCGA1165070.4106506292499126No Hit
CAGGAC1163110.4099597907309138No Hit
CTCGTC1154080.4067769989826698No Hit
TCCACG1146680.40416873110481755No Hit
ACGAAC1141980.4025121285337492No Hit
ATTCAT1133950.3996818054176473No Hit
GAGCAC1129570.3981379928088645No Hit
ACGTAA1128790.39786306727579357No Hit
ACTAGC1124830.3964672914925104No Hit
GCGTAT1113970.3926394821474462No Hit
TATGGT1110910.39156092813309107No Hit
ACCAGG1109640.3911132929702704No Hit
CCTTAT1107970.390524670354593No Hit
TCCAGA1100060.38773664347434816No Hit
CTCCGC1080570.3808670298338967No Hit
GAGTGA1078840.3802572591002907No Hit
CGAAGC1075020.3789108289255075No Hit
CCTCGG1073380.37833278036879425No Hit
TCCTCC1063990.375023099912979No Hit
TTAGTA1054740.3717627650656637No Hit
GTCGAG1049470.36990525537427427No Hit
GAATAC1046560.36887957165474045No Hit
GGTGGT1041290.3670220619633511No Hit
TTCGTT1033830.3643926459675703No Hit
CTTCAC1033670.3643362509864275No Hit
CATTAA1033200.3641705907293207No Hit
TGATAA1014340.3575230323271188No Hit
GCTCAA1012280.3567969469449059No Hit
TCATGT1009700.355887577873979No Hit
GAGTCG1003310.35363530331459037No Hit
AAGCGC1002790.3534520196258764No Hit
CCGGAA986790.3478125215116012No Hit
AACAAT974820.34359347198485907No Hit
AGGTGT968660.3414222652108631No Hit
ATGGCC966720.34073847606450725No Hit
TGACAC964160.3398361563662232No Hit
AGAGGT960130.3384157077786902No Hit
GGCCGT954980.33660049432315786No Hit
CCTGAC954850.33655467340097933No Hit
GACTTG952640.3357757177239451No Hit
AACATA952210.33562415621212394No Hit
CGTCTC945230.3331639251597714No Hit
GGTAAT943380.3325118581903083No Hit
ATGCCG939860.33127116860516775No Hit
AATGTA938300.33072131753902595No Hit
CGTGAA935990.32990711499877745No Hit
CCATGA931580.32835272833103035No Hit
TCGTGG923040.325342646212536No Hit
CAGCCT914780.32243125531104144No Hit
CCGCTC913450.32196247203029227No Hit
GCCATA906590.3195445372137968No Hit
NNNNNN902260.3180183480366211No Hit
GCCTGG883530.3114166105565977No Hit
TTAGCG870590.3068556664566776No Hit
TGCGGA854090.30103993402633134No Hit
GGCAGG851250.30003892311104746No Hit
GTCTGC849870.29955251639869124No Hit
CAAGGA835450.2944699187232007No Hit
AAGGCG833290.29370858647777354No Hit
GGCCAG825110.29082539306685035No Hit
TGTGAT809480.2853163083464678No Hit
CATGAG805760.28400512503489883No Hit
AACTCT794150.27991296421572787No Hit
GACACG792580.2793595884632646No Hit
ATTGAA790110.2784889909418734No Hit
TACAGT778880.27453076820291644No Hit
CAGTAG762480.26875028263578443No Hit
TCTCGC737190.25983635092890817No Hit
CCACTT699030.24638614792636182No Hit
TCAATG691380.24368976289047398No Hit
CGTTGC688060.2425195670317619No Hit
AGGACT683780.2410110012861933No Hit
AAGAGG648830.22869222259282343No Hit
GCTTCT641980.22627781246264936No Hit
GTACTT636460.2243321856132244No Hit
CTGAGG609180.2147168413283852No Hit
GCGGCT515190.18158831459333985No Hit
GTTGGC507590.17890955298905914No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)