FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24510462
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTA3402751.3882847251104447No Hit
TTGAAT3166171.291762676688836No Hit
GTACGC3104671.2666713503809108No Hit
CGTGAA2901931.1839556512643459No Hit
TTCTGT2839951.158668490214505No Hit
AATTGC2836811.1573874046111412No Hit
TTAGCG2820141.1505862272200336No Hit
CGTCAG2811581.147093840989207No Hit
CTTCAC2781451.134801131043552No Hit
CTCGTC2762001.1268657441055172No Hit
TTACGT2724991.1117660695257396No Hit
AAGTTG2640411.0772583560440436No Hit
TCTCTG2628851.0725420026762449No Hit
CAGCCT2596891.0595026727770371No Hit
CATTAA2558201.0437175766005553No Hit
CCTGAC2542491.0373080686932787No Hit
AAGGCG2515311.026218926432313No Hit
AGTTCC2458611.0030859475435427No Hit
ATTGCT2450660.9998424346305672No Hit
TGTGTG2381670.9716952703706686No Hit
ACCTGC2356260.9613282687205161No Hit
CTAGTT2325410.9487418066619879No Hit
ATACCT2313620.943931615813688No Hit
CACCTA2309280.9421609433555352No Hit
CGCTTG2286840.933005669170985No Hit
GATTCA2274150.9278282881815937No Hit
GACCGA2243270.9152295864516956No Hit
TATAAG2238820.9134140351985206No Hit
ATTCGA2228140.9090567121909002No Hit
GACCAT2216910.9044749952081688No Hit
GGAGGC2191800.8942303902717134No Hit
GAGAAG2181160.8898893868259196No Hit
ATCAGC2171520.885956372425783No Hit
AATGTA2148830.8766991009798183No Hit
CTGTAT2142410.8740798113066984No Hit
AGATGC2087860.8518240088660916No Hit
GCACAT2059880.8404084753685999No Hit
CCTGCT2057830.8395720978250022No Hit
TTCGTT1987350.8108170298870744No Hit
TGACAC1954180.797284033242621No Hit
TGTGAT1911930.7800464960635992No Hit
AGGCTT1894150.7727924508318121No Hit
GCCTGG1843520.7521359654501821No Hit
CATAAC1836720.7493616399397123No Hit
GGCAGG1819940.7425155837535825No Hit
ATTGAA1754480.7158086208248543No Hit
GTCTGC1731340.706367754308344No Hit
GCCAAT1728180.7050785089240669No Hit
GACTAA1724380.7035281505505689No Hit
AGGACT1720640.7020022715198106No Hit
ATTCAT1715500.699905207825132No Hit
CAAGCT1679800.6853399988951656No Hit
GCTTCT1625200.663063797002276No Hit
ACTAGC1603360.6541533162451202No Hit
ACTAAT1588530.6481028386980221No Hit
CTATTA1580580.6448593257850463No Hit
TTAGGC1577020.6434068847825063No Hit
ACAGTG1570520.6407549559857337No Hit
TGATAA1563060.6377113577051302No Hit
TGACCA1551150.6328522081713515No Hit
CCGCTC1532290.6251575347702545No Hit
CGATGT1501210.6124772352312249No Hit
CGTTGC1487330.6068143472775014No Hit
TCAATG1439930.5874756665133444No Hit
GTGGTG1407110.5740854660348712No Hit
TATTCT1404730.5731144521062067No Hit
GAGCAC1362950.5560686697786439No Hit
TTCTAC1348280.550083470478851No Hit
GTCTCA1345820.549079817426534No Hit
TCCTCC1334830.5445960178147601No Hit
TGCGGA1330490.5428253453566073No Hit
GTTACA1312000.5352816279024034No Hit
TCGTGG1309590.5342983743023694No Hit
AGTCAA1303870.5319646769612094No Hit
AGCCTA1292410.5272891224979765No Hit
CCACTT1287880.525440932121149No Hit
TAGAGC1283880.5238089759385197No Hit
GCGTAT1279100.5218587883002777No Hit
ATCCAC1270450.5183296830553418No Hit
GGCGTT1269570.5179706526951634No Hit
GGATTC1243680.5074078163030954No Hit
GTACTT1230030.501838765829873No Hit
GCATAA1216100.49615547842386654No Hit
GGCTAC1201360.4901417198908776No Hit
GGTGGT1199340.48931758201864983No Hit
AATCGG1163860.47484213067872816No Hit
CCATGA1160630.473524326061255No Hit
CCGACT1149680.46905684601130737No Hit
CACATT1118080.45616439216853605No Hit
TATGGT1109150.45252104999081616No Hit
CAAGGA1106280.4513501214297797No Hit
CTGAGG1100220.44887770781309627No Hit
AACTCT1099320.4485105176720047No Hit
GAATAC1071570.43718882165501405No Hit
AAGAGG1057860.4315952918390522No Hit
TTAGTA1050500.42859249246301434No Hit
GCGGCT1039960.42429228792178625No Hit
ACGTAA1032740.42134660701214033No Hit
AACATA1007040.41086128853874726No Hit
TCATGT1006760.4107470516059632No Hit
GATCCT995370.4061000563759264No Hit
TACAGT992100.40476593219662693No Hit
AGTAGG960350.39181227999700696No Hit
AGACGG944430.38531709439014245No Hit
GAGTGA927660.3784751180944692No Hit
CGTCTC917320.37425651136237253No Hit
GAGCCA894640.3650033198068645No Hit
TACTCG887980.36228611276278677No Hit
GGATCG884120.36071127504654954No Hit
GTAAGA883530.3604705615096117No Hit
CAGATC883290.36037264413865394No Hit
ACCGCT869060.3545669600189503No Hit
TTCCAT858920.35042995109598507No Hit
ATGCCG853510.348222730358979No Hit
AACTAG833620.34010782824085484No Hit
TACGAA825060.3366154420100282No Hit
AACAAT821430.33513444177429214No Hit
TACGGC820130.33460405601493765No Hit
CGAATT811000.3308791160280863No Hit
CTGGAG809580.3302997715832529No Hit
ACAGCC803800.3279415948993536No Hit
TATTGG801210.32688490327110115No Hit
GCTGTC767260.3130336751710351No Hit
AGGTGT745350.30409463518068325No Hit
GAGATA736410.30044721311250683No Hit
TGCGAC735900.3002391386992216No Hit
CCTTAT731460.2984276673365031No Hit
CAACGG728640.2972771382277494No Hit
AAGCAT726350.2963428433131942No Hit
CTCCGC722890.2949312012152199No Hit
GGACTA721410.29432737742764703No Hit
ACCAGG714000.2913041785993263No Hit
GATCAG713100.2909369884582347No Hit
CAGTAG706910.2884115362656159No Hit
GGTACC699490.28538425754683855No Hit
TAAGTC693450.28292000371106835No Hit
CGAGAG690470.28170419635500954No Hit
AGTGCA689350.28124724862387335No Hit
CATGAG671760.2740707213107611No Hit
TAATGA668510.2727447569123748No Hit
ACTTGA666730.2720185364111048No Hit
TCAAGC659670.2691381337487641No Hit
ATGTGA657460.26823647795786143No Hit
CAGGAC652850.2663556484573812No Hit
TTCACA652690.266290370210076No Hit
TCGGAC647430.2641443478299185No Hit
CCTCGG645030.26316517412034096No Hit
GAACCG635380.2592280798297478No Hit
GAGTCG619860.2528960898411462No Hit
AGGTCA618780.2524554616718363No Hit
GACACG615480.25110909782116714No Hit
TCTCGC610610.24912219116881598No Hit
AGAGGT606580.247477995314817No Hit
TAGCTT606450.24742495673888157No Hit
CGACAA605210.24691905032226646No Hit
GGCCAG595300.24287587887980244No Hit
GAAGAT590990.24111744609301936No Hit
ACACGA569830.23248439788691047No Hit
CTACCG555490.22663383497218453No Hit
CGAGCC553290.22573625907173844No Hit
GTTGGC508070.2072869944271144No Hit
AGAACG489250.19960864058784367No Hit
ATCCGG487960.19908233471894574No Hit
AGTTAG471720.19245659261747086No Hit
ATGGCC455800.18596140701060632No Hit
GCCATA439820.17944174206100236No Hit
CTATGC434300.17718964252897396No Hit
TGGCAT432990.17665517687916285No Hit
CGAAGC402430.16418703164387516No Hit
CTTAGC401100.16364440621315096No Hit
TCCACG397070.16200021035915194No Hit
AACCTT384470.1568595483838697No Hit
CAGTCC365710.1492056738873384No Hit
TGCATA359190.14654558530965267No Hit
ACGAAC331900.13541156425366443No Hit
TCCAGA324860.13253932137223687No Hit
GGCCGT320580.13079312825682354No Hit
AAGCGC315740.12881846127584212No Hit
CATACG307780.12557086847240986No Hit
CGTACT299300.12211112136523579No Hit
GGTAAT298130.12163377418181673No Hit
CCGTCG293130.11959382895353013No Hit
TCTTCA287390.11725197183145711No Hit
CTGTTC282090.11508962988947331No Hit
GCGCAG270100.11019784123204206No Hit
GACTTG256620.10469814889658138No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)