FastQCFastQC Report
Thu 9 May 2024
EGAF00001836234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27965087
Total Bases167.7 Mbp
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA3706031.325234568374488No Hit
CCTGCT3224961.1532093570815638No Hit
GCACAT3178751.1366851817768349No Hit
GAATAC2538860.9078677280710765No Hit
TTGAAT2423930.8667700551047812No Hit
TTAGCG2398020.8575049310592168No Hit
GGCGTT2373620.84877976599894No Hit
AGCCTA2334810.8349017473108523No Hit
GGTGGT2212610.7912044042630727No Hit
CGTCTC2198860.7862875591983676No Hit
AAGCAT2167520.7750807283381597No Hit
CGTGAA2155040.7706180209630673No Hit
GACTAA2122250.7588926864414904No Hit
ACCTGC2121860.7587532268360188No Hit
CCGACT2120940.7584242452025985No Hit
CACCTA2110010.7545158003620729No Hit
CACATT2087930.7466202411599864No Hit
TTACGT2060410.7367793992559366No Hit
TCAAGC2042670.730435775150637No Hit
CCTGAC1997930.7144372552819164No Hit
ATGCCG1992340.7124383342701562No Hit
ACGCTA1986050.7101891011460111No Hit
TAAGTC1984170.7095168343298914No Hit
GATCCT1975260.7063307187279625No Hit
TAATGA1942280.6945374423473096No Hit
CAGCCT1932330.6909794344641231No Hit
AGATGC1926640.688944754579165No Hit
AAGGCG1908350.6824044566712772No Hit
AAGTTG1896550.6781849096339303No Hit
CTTCAC1894190.6773410002264609No Hit
CCGCTC1870050.6687088082364986No Hit
TATTCT1835420.6563255104480813No Hit
TCGTGG1816450.649542052202448No Hit
TACTCG1815210.6490986421747946No Hit
ATCCGG1814150.6487195981188973No Hit
GGTACC1776390.6352170475993871No Hit
GAGCCA1774950.634702119825338No Hit
AGGCTT1766500.6316804950401191No Hit
AGACGG1765580.6313515134066989No Hit
TGCGAC1759020.6290057313249195No Hit
GGCTAC1756640.6281546701428106No Hit
GGATCG1729880.6185855956750644No Hit
CTCGTC1725880.6171552407471502No Hit
CCTTAT1716510.6138046343285112No Hit
CATTAA1707590.6106149428392624No Hit
GTACGC1693280.6054978480846492No Hit
TGTGTG1688300.6037170561993961No Hit
AGTCAA1686440.6030519411579159No Hit
GTTACA1686080.6029232092144037No Hit
CAGGAC1675610.5991792551905881No Hit
TTAGGC1675180.5990254920358374No Hit
ATCCAC1665620.5956069437581224No Hit
CAGTAG1650290.5901251084968911No Hit
AGTTCC1649960.5900071042153382No Hit
CATAAC1635640.5848864335734053No Hit
GAGAAG1617410.5783675909894362No Hit
AGGACT1610430.5758716216402259No Hit
TTCACA1608680.5752458413592634No Hit
CGTCAG1607760.5749168597258432No Hit
TTCTGT1607530.5748346143174881No Hit
CTGTAT1607370.5747774001203715No Hit
GCCAAT1595720.5706114913928213No Hit
TCCTCC1575570.5634060784434535No Hit
GTAAGA1566070.5600089854896572No Hit
GGATTC1564960.559612061997161No Hit
TATTGG1564100.5593045356876594No Hit
AAGAGG1562810.5588432462234071No Hit
TGACCA1554750.5559610810436599No Hit
CCATGA1541600.551258789218142No Hit
AGGTGT1531150.5475219869689659No Hit
TCTCTG1522040.5442643536206413No Hit
AATCGG1516060.5421259730034096No Hit
GAAGAT1501770.537016030023436No Hit
TGCGGA1497880.5356250098560394No Hit
ACTAGC1493270.5339765258016183No Hit
TTAGTA1492570.5337262136892333No Hit
TTCCAT1488200.532163550930487No Hit
ATGTGA1487570.5319382700293405No Hit
GCATAA1485780.5312981861990989No Hit
GCTTCT1482310.5300573532991333No Hit
CTGGAG1477940.528494690540387No Hit
AACATA1474690.5273325271614567No Hit
AGGTCA1466420.524375268347994No Hit
GACCGA1465530.5240570143765331No Hit
CTACCG1458080.5213929783232929No Hit
AACAAT1456790.5209316888590405No Hit
AATGTA1454720.5201914801838449No Hit
CGAATT1452270.5193153877904975No Hit
CAAGCT1419690.5076651469026362No Hit
AACCTT1409640.5040713801462516No Hit
AACTAG1405980.5027626053872102No Hit
ACACGA1402580.501546803698483No Hit
GCGTAT1398060.49993050262994No Hit
CGATGT1395230.49891852651844065No Hit
CGAGCC1387930.4963081287749972No Hit
TACGAA1371070.49027918275383875No Hit
CGAGAG1360900.48664250534961684No Hit
GGCCAG1357020.48525506106954003No Hit
ACAGTG1354390.48431460270443644No Hit
TACGGC1353660.4840535629300921No Hit
GGACTA1333620.4768874847412418No Hit
CGTTGC1328470.4750459027715523No Hit
TGGCAT1326810.47445230547646783No Hit
ACAGCC1322780.47301122288659425No Hit
TGCATA1310000.46844123889190836No Hit
TCAATG1307320.4674829010902058No Hit
CTCCGC1305610.46687142435852247No Hit
TTCGTT1296790.4637174917424716No Hit
GAGATA1288030.46058501445033945No Hit
TCTCGC1277680.4568839710743614No Hit
TATGGT1265650.45258217862865935No Hit
CTAGTT1265470.4525178126569033No Hit
ATTGAA1260330.4506798065745335No Hit
GTCTGC1259720.4504616774480265No Hit
TATAAG1244780.44511930179226694No Hit
AATTGC1244400.4449834180741151No Hit
ATTCAT1239130.443098925456588No Hit
AGAGGT1237480.4425089040488234No Hit
ATACCT1228230.43920120827802184No Hit
GAGCAC1228160.43917617706678336No Hit
TGACAC1213770.4340304752136119No Hit
CTATGC1209400.43246781245486565No Hit
CAGATC1209100.432360535835272No Hit
ACGAAC1202720.43007911972524887No Hit
CAACGG1202310.42993250834513763No Hit
GACCAT1197740.42829832783999566No Hit
GGAGGC1194270.4270574949400301No Hit
AGTAGG1190000.4255305910544816No Hit
ACGTAA1182200.42274139894504886No Hit
ATCAGC1173510.41963395286415517No Hit
TGTGAT1171970.4190832662169083No Hit
GCGGCT1165010.4165944486423375No Hit
GTCTCA1155130.4130614719703894No Hit
AGTGCA1153420.412449995238706No Hit
GAACCG1151680.4118277908450633No Hit
ACTAAT1141840.40830911772239437No Hit
GCTGTC1130250.4041646643187629No Hit
ATTGCT1128770.40363543299543464No Hit
ACCGCT1126530.4028344342358027No Hit
GGCAGG1113450.3981571736215232No Hit
CGCTTG1111530.3974706032561243No Hit
AGAACG1087980.38904938861802935No Hit
TGATAA1085230.3880660196050883No Hit
CTTAGC1082940.3872471414088574No Hit
TAGAGC1080520.3863817766774693No Hit
ATGGCC1072940.3836712540890718No Hit
GAGTGA1071100.3830132908222313No Hit
GATTCA1064020.38048156259982313No Hit
CATACG1063480.3802884646845547No Hit
CCTCGG1036410.3706085377098952No Hit
ACCAGG1035530.37029385962575406No Hit
AGTTAG1031000.3686739826698912No Hit
TCGGAC1030380.36845227765606453No Hit
CTGTTC1028320.3677156448681887No Hit
ATTCGA1019500.36456171225213785No Hit
GTACTT1017190.36373568228126735No Hit
CGACAA1005310.3594875281453621No Hit
CATGAG993840.35538598538956806No Hit
TCCACG988750.3535658587437972No Hit
GAGTCG985780.3525038202098209No Hit
CGTACT953640.3410109183640301No Hit
GCCATA948000.3389941179156711No Hit
CAAGGA927310.33159560705103475No Hit
AACTCT916170.32761206857679365No Hit
TCTTCA910230.325487991508841No Hit
CGAAGC898000.32111468131674326No Hit
GACACG895240.32012773641648246No Hit
GCGCAG872950.31215708358068045No Hit
GTGGTG858810.3071007789105037No Hit
GCCTGG836650.29917661260985884No Hit
TACAGT825180.29507506985406484No Hit
AAGCGC824450.29481403007972046No Hit
GATCAG820920.2935517418558362No Hit
GGCCGT811780.29028338084555216No Hit
TCCAGA811490.2901796801132784No Hit
TTCTAC788300.28188719741869567No Hit
CAGTCC783960.28033526232190875No Hit
GGTAAT765640.2737842367520616No Hit
CCACTT755590.2701904699956771No Hit
GTTGGC739070.26428310414339135No Hit
CCAGCG715710.2559298313643723No Hit
ACTTGA701820.25096292387719016No Hit
CCGGAA701480.25084134370831745No Hit
GCTCAA687600.2458780121084551No Hit
TAGCTT655290.23432432017822794No Hit
CTGAGG652490.233323071728688No Hit
CCGTCG606480.21687041417035466No Hit
GTCGAG600960.21489652436983298No Hit
TCATGT573580.20510574488826014No Hit
GACTTG551620.19725309633401106No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)